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SMC proteins Chromosomal organizers from bacteria to human

June 13-16, 2017 Nanyo city, Yamagata, Japan

Venue: Nanyo City Cultural Hall

430-2 Mitsumadori, Nanyo city, Yamagata, 999-2232, JAPAN

Organizers: Tatsuya Hirano (RIKEN)

Tomoko Nishiyama (Nagoya Univ.) Katsuhiko Shirahige (The Univ. Tokyo)

Yuri Nakagawa (The Univ. Tokyo)[administrative]

Sponsors Grant-in-Aid Scientific Research on Innovative Areas The Molecular Biology Society of Japan RIKEN Centennial RIKEN Symposium Series The Uehara Memorial Foundation Nanyo City

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Meeting Program Tuesday, June 13 13:00-14:20 Arrival & registration

14:20-14:30 Welcome address

KEYNOTE SESSION (Chair: Tatsuya Hirano)

14:30-15:15 William C. Earnshaw (Univ. of Edinburgh, UK) T01

Condensin – from non-histone scaffold to chromosomal

nano-machine

SESSION I: Prokaryotic SMC (I) (Chair: Tatsuya Hirano) 15:15-15:40 David Z. Rudner (Harvard Medical School, USA) T02

Bacillus subtilis SMC complexes juxtapose chromosome

arms as they travel from origin to terminus

15:40-16:05 Byung-Ha Oh (KAIST, Korea) T03

Structure and conformational change of full-length Smc

16:05-16:30 Stephan Gruber (Univ. of Lausanne, Switzerland) T04

DNA chambers in Smc-ScpAB

16:30-17:00 Afternoon coffee/tea

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SESSION II: Chromosome biophysics and modeling (Chair: Christian Haering) 17:00-17:25 Leonid Mirny (MIT, USA) T05

Chromosome folding by loop extrusion

17:25-17:50 Kerry Bloom (Univ. of North Carolina, USA) T06

Partitioning nuclear sub-domains

17:50-18:05 Yuji Sakai (RIKEN, Japan) T07

Modeling the functions of condensin in chromosome

shaping and segregation

18:05-18:30 John F. Marko (Northwestern Univ., USA) T08

Condensin in eukaryote mitotic chromosomes and

on single DNA molecules

18:30-18:45 Je-Kyung Ryu (TU Delft, The Netherlands) T09

High-speed atomic force microscopy imaging on condensin

19:30-21:00 Dinner (served at Takinami for all participants)

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Wednesday, June 14 7:00- Breakfast (served at each inn)

7:45- The first shuttle leaves for the Cultural Hall

SESSION III: Sister chromatid cohesion (I) (Chair: Jan-Michael Peters)

8:30-8:55 Douglas Koshland (UC Berkeley, USA) T10

Novel functions for the coiled coils of cohesin

8:55-9:20 Mitsuhiro Yanagida (OIST, Japan) T11

Cohesin clip model

9:20-9:35 Madhusudhan Srinivasan (Univ. of Oxford, UK) T12

The topology of cohesin’s association with chromosomes

and its regulation by HAWKs

9:35-10:00 Yasuto Murayama (Tokyo Inst. of Tech., Japan) T13

Cohesin mediates DNA-DNA tethering in vitro

10:00-10:30 Morning coffee/tea

SESSION IV: Condensin (Chair: John Marko) 10:30-10:55 Christian H. Haering (EMBL, Germany) T14

Structural basis for a safety-belt mechanism that anchors

condensin complexes to chromosomes

10:55-11:20 Frank Uhlmann (The Francis Crick Institute, UK) T15

Condensin-mediated remodeling of the mitotic chromatin

landscape

11:20-11:45 Toru Hirota (JFCR, Japan) T16

Cooperative acts of condensins and topoisomerases in

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shaping mitotic chromosomes

11:45-12:10 Giovanni Bosco (Geisel Sch. Med., Dartmouth, USA) T17

Human condensin II subunit NCAPH2 associates with

shelterin protein TRF1 and is required for telomere stability

12:10-13:00 Lunch

13:00-15:30 Poster Session 1 (Odd Number) SESSION V: Sister chromatid cohesion (II) (Chair: Frank Uhlmann) 15:30-15:55 Hongtao Yu (Univ. of Texas, USA) T18

Structural and mechanistic insight into cohesin loading

and release

15:55-16:10 Benjamin D. Rowland (NKI, The Netherlands) T19

The cohesin release factor WAPL restricts chromatin loop

extension

16:10-16:35 Dale Dorsett (Saint Louis Univ., USA) T20

Roles of Pds5, Wapl and Brca2 in cohesin localization

and sister chromatid cohesion

16:35-16:50 Tomoko Nishiyama (Nagoya Univ., Japan) T21

Cohesin dynamics on single DNA in Xenopus egg

extracts

16:50-17:15 Jan-Michael Peters (IMP, Austria) T22

How cohesin is positioned in mammalian genomes to

control chromatin architecture

17:15-17:45 Afternoon coffee/tea

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SESSION VI: Prokaryotic SMC (II) (Chair: Stephan Gruber) 17:45-18:10 David Sherratt (Univ. of Oxford, UK) T23

How does MukBEF work?

18:10-18:35 Hironori Niki (NIG, Japan) T24

Multiple cis-acting rDNAs near replication origin are

contributed for nucleoid separation as loading sites

for the Smc-ScpAB complex

18:35-18:50 Jan Palecek (Masaryk Univ., Czech Republic) T25

KITE proteins: new insights to evolution and dynamics

of SMC complexes

19:30-21:00 Dinner (BBQ at Hygeia Park for all participants)

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Thursday, June 15 7:00- Breakfast (served at each inn)

7:45- The first shuttle leaves for the Cultural Hall

SESSION VII: Chromosome organization in meiosis/zygotes (Chair: Kei-ichiro Ishiguro)

8:30-8:55 Franz Klein (MFPL, Austria) T26

The meiotic DSB machinery takes the Rec8-bus to work

8:55-9:10 Stefan Galander (Univ. of Edinburgh, UK) T27

Spo13 establishes meiosis I-specific chromosome

segregation by regulating cohesin protection and

mono-orientation

9:10-9:35 Yasushi Hiraoka (Osaka Univ., Japan) T28

A cohesin-based platform of the chromosome for

homologous chromosome pairing in fission yeast meiosis

9:35-10:00 Tomoya Kitajima (RIKEN CDB, Japan) T29

The causes of chromosome segregation errors in oocytes

10:00-10:25 Kikuë Tachibana-Konwalski (IMBA, Austria) T30

Single-nucleus Hi-C reveals unique chromatin

reorganization during the oocyte-to-zygote transition

10:25-12:55 Poster Session 2 (Even Number) 12:55-17:30 Lunch and excursion

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SESSION VIII: SMC and genome stability (Chair: Lena Ström) 17:30-17:55 Luis Aragón (Imperial College London, UK) T31

A phosphorylation-code at separase sites regulates

cohesin cleavage during DNA damage

17:55-18:20 Camilla Sjögren (Karolinska Institute, Sweden) T32

The Smc5/6 complex - connecting DNA supercoiling

and sister chromatid entanglement?

18:20-18:35 Johanne M. Murray (Univ. of Sussex, UK) T33

The Smc5/6 complex and replication stress

19:30-21:00 Dinner (served at Takinami for all participants)

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Friday, June 16 7:00- Breakfast (served at each inn)

7:45- The first shuttle leaves for the Cultural Hall

SESSION IX: SMC and gene regulation (Chair: Katsuhiko Shirahige)

8:30-8:45 Clémence Hocquet (CNRS, LBMC ENS de Lyon, France) T34

Role of condensin in the regulation of gene expression

In fission yeast

8:45-9:10 Barbara J. Meyer (UC-Berkeley, USA) T35

Dynamic control of X-chromosome conformation and

repression by condensin

9:10-9:35 Jennifer L. Gerton (Stowers Inst. for Med. Res., USA) T36

Condensin II is anchored by TFIIIC and H3K4me3 in the

mammalian genome and supports expression of active

dense gene clusters

9:35-10:00 Matthias Merkenschlager (MRC London Inst. Med. Sci., UK) T37

Cohesin regulates inducible and developmental genes

10:00-10:25 Ana Losada (CNIO, Spain) T38

Distinct contributions of cohesin-SA1 and cohesin-SA2

to genome architecture and gene regulation

10:25-10:55 Morning coffee/tea

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SESSION X: SMC and disease (Chair: Kerstin Wendt) 10:55-11:20 Andrew Wood (Univ. of Edinburgh, UK) T39

T cell lymphoma and tissue-specific chromosomal

instability in Caph2nes/nes mutant mice

11:20-11:35 Julia Horsfield (Univ. of Otago, New Zealand) T40

Cohesin mutations in myeloid leukaemia: the search for

mechanism

11:35-12:00 Ayana Kon (Kyoto Univ., Japan) T41

The genetic and biological characterization of pathway

mutations of cohesin complex in myeloid neoplasms

12:00-12:25 Matt Deardorff (Children's Hospital of Philadelphia, USA) T42

SMC1A mutations cause mechanistically separable allelic

disorders: Atypical Cornelia de Lange Syndrome and a

Rett-like Epileptic Encephalopathy

12:25-12:30 Closing remarks

12:30-13:30 Lunch and departure

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