Prosedur PembuatanReagent Untuk Pewarnaan .www.AlatAlatLaboratorium.com/Blog

Daftar IsiAcid Fast Stain / Pewarnaan Bakteri Tahan Asam.Capsule Stain / Pewarnaan Kapsul.Fungal Stain / Pewarnaan Jamur.Gram Stain / Pewarnaan Gram.

Daftar IsiNegative Stain / Pewarnaan Negatif.Spore Stain / Pewarnaan Spora.

Acid-fast Stain

Solution ABasic Fuchsin (90% dye content) = 0,3 gEthyl alcohol (95%) = 10,0 mlSolution BPhenol = 5,0 gDistilled water = 95,0 mlNote : Mix solutions A and B. Add 2 drops of trition X per 100 ml of stain for use in heatless method

Ethyl alcohol (95%) = 97,0 mlHydrochloric acid = 3,0 ml

Methylene Blue = 0,3 gDidtilled water = 100,0 ml

Capsule Stain

Crystal Violet (85% dye Content) = 1,0 gDistilles Water = 100,0 ml

Copper Sulfate (CuSO . 5HO) = 20,0 gDistilled water = 80,0 ml

Fungal Stains

Lactic acid = 20,0 mlPhenol = 20,0 gGlycerol = 40,0 mlDistilled water = 20,0 mlAniline Blue = 0,05 gNote : Heat Gently in hot water (double boiler) to dissolve; then add aniline blue dye

Gram`s iodine (as in Gram`s stain) = 10,0 mlDistilled water = 30,0 ml

Gram Stain

Solution ACrystal Violet (90% dye content) = 2,0 gEthyl alcohol (95%) = 20,0 mlSolution BAmmonium oxalate = 0,8 gDestilled water = 80,0 mlNote : mix solutions Aand B

Iodine = 1,0 gPotassium iodide = 2,0 gDistilled water = 300,0 ml

Ethyl Alcohol (100%) = 95,0 mlDistilled water = 5,0 ml

Safranin O = 0,25 mlEthyl alcohol (95%) = 10,0 mlDistilled water = 100,0 ml

Negative Stain

Nigrosin, water-soluble = 10,0 gDisrilled water = 100,0 mlNote : Immerse in boiling water bath for 30 minutes.Formalin = 0,5 mlNote : Filter twice through double filter paper

Spore Stain

Malacite green = 5,0 gDistilled water = 100,0 ml

Safranin O = 0,25 mlEthyl alcohol (95%) = 10,0 mlDistilled water = 100,0 ml

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