Study on application of Chinese Biological Pesticide

Study on application of Chinese Biological Pesticide

Speaker: Kelley ZhouBeijing Multigrass Formulation Co.,Ltd

Overview of biological pesticides

Introduction of popular biological pesticides

Problem and suggestion of biological pesticides

3Rs

Food securityenvironment

security

3

BIO-PESTICIDES BACKGROUND

ADVANTAGES OF BIO-PESTICIDE

选择性强 Selective

4

对生态环境影响小 Environmental friendly

产品寿命长 Long life of products

原材料来源广泛 Rich Raw Material

DEVELOPMENT TREND OF GLOBAL MARKET

1. 应用会更加广泛 Extensive Application

2. 市场份额会逐年增加 More Market Share

3. 和化学农药共同发展，相辅相促Coexist with Conventional Pesticides

5

Advantages of Chinese Bio-pesticides

6

1. 几千年的历史经验Historical experience of

thousands years

2. 雄厚的理论和技术基础Deep theoretical and technical

foundation

3. 广泛的原材料来源Extensive Source of Raw

Material

7

Types of active ingredients

• More than 80

Numbers of products

• More than 2500

Yearly capacity of formulation

• More than 100 thousand MT

Application area

• 0.4-0.5 billion acres

7

DEVELOPMENT OF CHINESE BIO-PESTICIDE

General situation of Chinese bio-pesticide

Euphorbia Stemona Tripterygium Melia toosendan

Croton Sophora flavescens

Main products in the market

井岗霉素 Validamycin 阿维菌素 Abamectin 赤霉素 Gibberellin 苏云杆菌 BT

9

Main products in the market

Main bio pesticides in china

Introduction of some bio pesticide

• Matrine• Oligo sacchrin• Bioflavonoid Complex

Matrine

Matrine

• Matrine is a kind of alkaloid extracted from Sophora flavescens Ait. It can act as pestidide, fungicide and also stimulating plant growth and used for controlling the pests of Lepidoptera , mites ,etc.

Application of popular Chinese Bio pesticide

app:ds:alkaloid

app:ds:Lepidoptera

Activities and features of Sophora flavescens Ait extract

1. Insecticidal activity contact poison firstly, stomach

poison secondly. It can control and kill pests.

2. Bactericidal activityIt can inhabit fungus, bacterium and

virus.

3. Stimulate plant growth Amino acids, fatty acids and other

substances in it can stimulate and regulate the growth of crops.


Field trial collection of Matrine product• To control mites on citrus(0.5% matrine)• To control aphides on rose(0.3% matrine)


1. To control mites on citrusTest Condition

• Test place: Rio Grande do Sul in Brazil • Temperature: 17,9°C - 23,8°• Humidity: 73%---87%• Test period: October 2008

Test design• Treatment: 0.5% matrine SL 1000 times , 40% omite EC 1000

times and water control• Dosage: 1kg/ha for each concentration• Application times: one application for each treatment

Efficacy calculation We check the pests alive and dead number after 1 day,

2days, 3days and 5 days.


The mites observed on the leaf

Mites zoomed in

1. To control mites on citrus


Three days after application of 40% Omite 1000times

Three days after application of 0.5% matrine SL 1000times

Application of popular products in China



controlled by 0.5% matrine SL Control by omite 40% EC


Time Control 1day 2 days 3 days 5days

0.5% matrine1000 times 26% 72% 98% 100%

40% Omite EC 1000 times 49% 90% 75% 63%

Water comparision 0 4% 4% 10%



2.To control aphides on rosesTest Condition

• Test place: an indoor laboratory of Bogota City in Colombia • Test period: August 2006

Test design• Treatment: 0.3% matrine 1000 times(1kg/ha), 20% methomyl

1500 times(0.75kg/ha) and water control• Application times: one application for each treatment Efficacy calculation We check the pests alive and dead number after 1 days, 3

days, 7 days and 10 days. For each check, we chose 10 plants randomly and for each plant we checked 10 leaves.


It is in threshold before application

The bud covered by aphides

2.To control aphides on roses




3 days after application of 0.3% matrine1000 times



TreatmentPest density before application

1 day after application

3 days after application



Density Efficacy Density Efficacy Density Efficacy Density Efficacy

0.3% Matrine1000 times

13.8 0.1 99.1 0 100 0.1 99.8 0.3 99.4

20% methomyl EC 1500 times

8.9 0.2 98.5 0.1 99.7 0.3 99.1 0.6 98.1

Water control

12.2 18.1 0 42.1 0 46.4 0 43.0 0



Oligo SaccharinBased on halobios ， advanc

ed technology extract the low-

molecular carbohydrate

The animals & plants self immune

formulation

Supply nutrition Prevent and cure disease of crop

Human medicine, plants care, plants

nutrition


Improve solid environment

Induce plants to generate disease-resistant hormone

and active cells

Provide plants nutrition

Function

Target diseases: Downy Mildew, Fusarium Wilt, Verticillium Wilt, Ring Spot, Anthracnose, Angular leaf spot, soft rot, Root knot nematode, etc.


To control downy mildew on roseProduct: 3% Oligo sacchrin

Disease ： downy mildew

Level of disease ： moderate

Crop ： rose

Date ： 06/12/2007-21/12/2007

Method ： foliar spray, 7 days interval, 3 times

Dosage ： 1.5L/ha.,dilution rate is 600

Person in charge: Hernando

Location ： Colombia

Contrast: metalaxyl


Rose leaves infected by downy mildew


Rose leaves infected by downy mildew


The disease was controlled after applied with 3% Oligo saccharin


The disease was still alive after applied with metalaxyl.


Test result （ curative ）

Suggestion: When crops infected by downy mildew, dilute Baiclean with 600 time, foliar spray. 7 days interval, 3 times.


Use of Oligo saccharin at the beet crop

Objective: To evaluate performance in the control of nematode

• Products used: 3% Oligo saccharin x Nemix(nematocide) x Control • Technical Responsible: Ricardo Martins• Property: Farm Canada• City: Uberaba, State of Minas Gerais• Producer: Claudio Carvalho Ottoni• Date of installation of test: 18/12/2008 Pontecial of the producer:

• Carrot 650 ha to 700 ha / year• Beet 120 ha / year

Culture: • Variety: Beet Ferry Morse ： Ferry Morse• Destination of output: Fresh Consumption

Date of sowing / transplanting: 18 / 12 / 08 Date of collection: 08 / 03 / 08• Planting spacing: plots has a width of 1.75 m with 4 double rows, where we have

20 plants in double line, obtaining a final stand of 457,000 plants / ha.

Technique of cultivation: Irrigation Central Pivot. Methodology: The trial was to test an area of 1,000 m2 and the control is

in the central area.

The applications were as follows:– 1st application 03 days after sowing; – 2nd application 07 days after germination; – 3rd application 20 days after germination; – 4th application 40 days after germination; – 5th application 60 days after germination.

• All applications were isolated, because we do not know the compatibility, and used for all applications a flow of 400lts of water / ha with a dosage of 800ml/ha.

• Valuation:

• - 1st Rating: 19/01 - after thinning - 2nd Rating: 17/02

Control

(without nematicide)

3% oligo saccharin

Control (without nematicide)

NEMIX (Standard)

One Application

3% oligo saccharin

Four Application

15% Bioflavonoid

Complex


Test informationProduct

Contrast

Disease

15% Bioflavonoid ComplexKresoxim-methylPowdery mildew

Disease level

Crop

Date

Moderate Rose

23/03/2009-14/04/2009Method

Dosage

Person in charge

Foliar spray0.75-1L/ha., dilution rate1000-1500

MuradLocation Ethiopia

Overview of rose plantation Application of popular Chinese Bio pesticide

When the buds of rose infected by powdery mildew, they would not open or even they will not come into flower.


New leaves sprouted after applied with 15% Bioflavonoid Complex. After recovery, the white powder was removed, there were yellow spot on the leaves.


Test resultProduct Dosage （ fungicide/water

） /dilution rate

Disease Crop % efficacy

15% Bioflavonoid Complex

1.25 cc/L800 times

Powdery mildew

Rose 72%

15% Bioflavonoid Complex

1.5 cc/L600 times

Powdery mildew

Rose 85%

Kresoxim-methyl

1.5 cc/L600 times

Powdery mildew

Rose 76%


46

1. 认知的误区 (acknowledge)

2. 生产污染 (Pollution)

3. 使用方法 (Application)

Problems

Problems of bio pesticides

47

1. 发掘历史沉淀 (Histrorical resource)

2. 重视制剂的研发 (Formulation)

3. 加大登记的投入 (registration)

Suggestion

THANKS FOR YOUR ATTENTION!

BEIJING MULTIGRASS FORMULATION CO.,LTD

Documents

Study on application of Chinese Biological Pesticide