Supplementary Information Supplementary tables · PDF fileqGW8/qgw8 165 2.46 ± 0.01 8.53 ± 0.02 3.47 ± 0.01 ... Nature Genetics: doi:10.1038/ng.2327. 2 Supplementary Table 3 Yield

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Supplementary Information

Supplementary tables

Supplementary Table 1 Effects of the QTL on grain length and width detected in SSSLs derived from Basmati385 and HJX74

QTL Chromosome Marker interval Additive

effect

Percentage of phenotypic

variance (%) qGW1 1 RM580-RM562 -7.10 ± 3.48 17.84 ± 8.73 qGW4 4 RM401-RM471 -6.60 ± 5.08 16.58 ± 12.75qGW8 8 RM80-RM447 -17.71 ± 0.63 44.49 ± 1.58 qGW10 10 RM258-PSM168 -5.11 ± 2.36 12.85 ± 5.94 qGL1 1 RM294b-RM488 14.85 ± 1.23 13.88 ± 1.14

qGL3.1 3 RM569-RM545 16.67 ± 3.69 12.73 ± 2.81 qGL3.2 3 RM282-PSM127 29.49 ± 5.46 26.21 ± 5.94 qGL7 7 RM336-RM18 12.93 ± 0.49 12.09 ± 0.46

Supplementary Table 2 Effects of natural variation in the GW8 locus on grain size and

shape

Genotype No. of plants GW* (mm) GL* (mm) LWR* qGW8/qGW8 79 2.64 ± 0.01 8.14 ± 0.02 3.08 ± 0.01qGW8/qgw8 165 2.46 ± 0.01 8.53 ± 0.02 3.47 ± 0.01qgw8/qgw8 83 2.24 ± 0.01 8.81 ± 0.02 3.95 ± 0.02

*GW, grain width; GL, grain length; LWR, length/width ratio.

Nature Genetics: doi:10.1038/ng.2327

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Supplementary Table 3 Yield test in paddies among NIL-GW8, NIL-gw8 and NIL-gw8Amol plants

Actual yield per plot ( Kg) in 2008ψ



Hainan NIL-GW8 1.10 ± 0.02 bB¶ 1.09 ± 0.02 bB 1.19 ± 0.02 bB NIL-gw8§ 1.01 ± 0.01 aA 0.99 ± 0.01 aA 1.03 ± 0.02 aA NIL-gw8Amol* 1.09 ± 0.02 bB 1.06 ± 0.02 bAB 1.12 ± 0.04 bB Beijing NIL-GW8 1.17 ± 0.02 bB 1.21 ± 0.01 cB 1.28 ± 0.02 cB NIL-gw8§ 1.03 ± 0.01 aA 1.03 ± 0.01 aA 1.06 ± 0.02 aA NIL-gw8Amol* 1.17 ± 0.02 bB 1.07 ± 0.01 bA 1.24 ± 0.01 bB

ψData given as mean ± SE (n=40). Plants were grown in randomized complete block (each block contain 10 plots, and each plots contains 40 plants) design with 4 replications, respectively. §Multiway ANOVA revealed a significant difference in grain yield between NIL-GW8 and NIL-gw8 plants (P <0.05), and this effect was consistent across 3 years and across 2 locations. *Multiway ANOVA showed no significant differences in grain yield between NIL-GW8 and NIL-gw8Amol plants (P <0.01), except Beijing in 2009. ¶Values in the same column with the same letters do not differ significantly, the values with different subscript letter differ significantly (with small letter, P <0.05) or very significantly (with capital letter, P <0.01).


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Supplementary Table 4 Effect of OsSPL16 on the physicochemical characteristics of milled rice Physicochemical Characteristics*

NIL-GW8 NIL-GW8

pActin::RNAi-OsSPL16NIL-gw8

NIL-gw8 pOsSPL16::OsSPL16HJX74

NIL-gw8 pOsSPL16::OsSPL16Basmati385 Basmati385

Basmati385 pOsSPL16::OsSPL16Basmati385

GW (mm) 2.12 ± 0.01 1.83 ± 0.01 1.76 ± 0.01 1.87 ± 0.03 1.87 ± 0.01 1.67 ± 0.01 1.79 ± 0.01

GL (mm) 5.43 ± 0.01 5.70 ± 0.01 5.90 ± 0.01 5.63 ± 0.02 5.70 ± 0.01 6.62 ± 0.01 5.82 ± 0.01

LWR 2.56 ± 0.01 3.14 ± 0.01 3.35 ± 0.01 3.01 ± 0.01 3.05 ± 0.02 3.96 ± 0.01 3.25 ± 0.01

GET 0.54 ± 0.01 0.59 ± 0.01 0.69 ± 0.01 0.52 ± 0.01 0.52 ± 0.01 0.74 ± 0.03 0.57 ± 0.01

PGWC (%) 57.00 ± 0.57 49.67 ± 0.88 36.00 ± 0.57 65.67 ± 3.18 57.00 ± 0.57 35.61 ± 1.42 71.67 ± 0.88

SGE (%) 9.15 ± 0.11 7.35 ± 0.04 5.87 ± 0.11 15.55 ± 0.48 11.36 ± 0.09 7.36 ± 0.13 14.73 ± 0.19

AC (%) 27.27 ± 0.22 26.01 ± 0.25 25.94 ± 0.09 24.26 ± 0.16 24.67 ± 0.44 21.69 ± 0.23 25.47 ± 0.38

GC (mm) 38.50 ± 0.50 34.50 ± 0.50 37.25 ± 0.94 39.67 ± 2.60 47.00 ± 1.73 61.25 ± 1.65 32.50 ± 2.50

GT (ASV) 6.89 ± 0.04 6.83 ± 0.11 7.00 ± 0.00 6.87 ± 0.05 6.91 ± 0.07 6.93 ± 0.01 6.96 ± 0.02

*GW, grain width; GL, grain length; LWR, length/width ratio; GET, grain endosperm transparency; PGWC, percentage of grains with chalkiness;

SGE, square of chalky endosperm; AC, amylose content; GC, gel consistency; GT, gelatinization temperature


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Supplementary Table 5 Sequence and haplotype analysis on the GW8 locus in cultivated varieties and wild rice accessions

Genotype Accession Origin Variety group1 Haplotype -33_-23 36 236 327-328 1007-1008

Oryza rufipogon DX-06 Jiangxi, China Basmati type ---------- C C GCG --

P25 Guangdong, China HJX74 type GAGCTGAGCT T T --- --

P61 Guangxi, China Basmati type ---------- C C GCG --

P46 Hainan, China HJX74 type GAGCTGAGCT T T --- --

80506 Madhya Pradesh, India HJX74 type GAGCTGAGCT T T --- --

105426 Akuressa, Sri Lanka Basmati type ---------- C C GCG --

81982 Medinipur, India HJX74 type GAGCTGAGCT T T --- --

81991 Ayeyawady, Myanmar HJX74 type GAGCTGAGCT T T --- --

105958 Kromat Watu, Indonesia Basmati type ---------- C C GCG --

106161 Vientiane, Laos Basmati type ---------- C C GCG --

VOC4 Nepal Basmati type ---------- C C GCG --

Oryza nivara 80470 Madhya Pradesh, India HJX74 type GAGCTGAGCT T T --- --

105705 Kohalpul Panchayat, Nepal HJX74 type GAGCTGAGCT T T --- --

105879 Avoynagar, Bangladesh Basmati type ---------- C C GCG --

89215 Sopoi Tep, Cambodia Basmati type ---------- C C GCG --

105784 Thailand HJX74 type GAGCTGAGCT T T --- --

Landraces 8555 Bangladesh AUS Basmati type ---------- C C GCG --

12883 Iran AUS Basmati type ---------- C C GCG --

45975 India AUS Basmati type ---------- C C GCG --

32399 Bhutan AUS Basmati type ---------- C C GCG --

6307 India AUS Basmati type ---------- C C GCG --

30416 Brazil IND Basmati type ---------- C C GCG --

9177 India IND Basmati type ---------- C C GCG --

8231 Vietnam IND HJX74 type GAGCTGAGCT T T --- --

2540 Japan IND Basmati type ---------- C C GCG --

9148 Thailand IND HJX74 type GAGCTGAGCT T T --- --

9060 India ARO Basmati type ---------- C C GCG --

9062 India ARO Basmati type ---------- C C GCG --

38994 Brazil ARO Basmati type ---------- C C GCG --

55471 South Korea TEJ Basmati type ---------- C C GCG --

27630 Nepal TEJ Basmati type ---------- C C GCG --

66756 TX, USA TRJ Basmati type ---------- C C GCG --

50448 Brazil TRJ Basmati type ---------- C C GCG --

38698 Pakistan TRJ Basmati type ---------- C C GCG --

Modern cultivars Basmati385 Pakistan indica Basmati type ---------- C C GCG --

Basmati Dhan Pakistan indica Basmati type ---------- C C GCG --

Basmati Kamon India indica Basmati type ---------- C C GCG --

Basmati Hunar India indica Basmati type ---------- C C GCG --


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Basmati-D India indica Basmati type ---------- C C GCG --

Basmati T3 India indica Basmati type ---------- C C GCG --

Basmati Bahar Pakistan indica Basmati type ---------- C C GCG --

Basmati 1 India indica Basmati type ---------- C C GCG --


Basmati 122 Pakistan indica Basmati type ---------- C C GCG --




Basmati 372A Pakistan indica Basmati type ---------- C C GCG --

Basmati 372B Pakistan indica Basmati type ---------- C C GCG --


Basmati 375A India indica Basmati type ---------- C C GCG --








Super Basmati Pakistan Indica Basmati type ---------- C C GCG --

HJX74 Guangdong, China Indica HJX74 type GAGCTGAGCT T T --- --

Dee-geo-woo-gen Taiwan, China indica HJX74 type GAGCTGAGCT T T --- --

Guangchangai Guangdong, China indica HJX74 type GAGCTGAGCT T T --- --

Nantehao Guangdong, China indica HJX74 type GAGCTGAGCT T T --- --

Lucaihao Guangdong, China indica HJX74 type GAGCTGAGCT T T --- --

Aizizhan Guangdong, China indica HJX74 type GAGCTGAGCT T T --- --

Lianjian33 (LJ33) Guangdong, China indica HJX74 type GAGCTGAGCT T T --- --

Qingerai Guangdong, China indica HJX74 type GAGCTGAGCT T T --- --

Teqing Guangdong, China indica HJX74 type GAGCTGAGCT T T --- --

Guichao2 Guangdong, China indica HJX74 type GAGCTGAGCT T T --- --

Guangluai4 Guangdong, China indica HJX74 type GAGCTGAGCT T T --- --

Fengaizhan Guangdong, China indica HJX74 type GAGCTGAGCT T T --- --

Fenghuazhan Guangdong, China indica HJX74 type GAGCTGAGCT T T --- --

Zhaiyeqing Guangdong, China indica HJX74 type GAGCTGAGCT T T --- --

Huanghuazhan Guangdong, China indica HJX74 type GAGCTGAGCT T T --- --

9311 Jiangsu, China indica HJX74 type GAGCTGAGCT T T --- --

Nanjing11 Jiangsu, China indica HJX74 type GAGCTGAGCT T T --- --

Yangdao2 Jiangsu, China indica HJX74 type GAGCTGAGCT T T --- --

Minghui63 (MH63) Fujiang, China indica HJX74 type GAGCTGAGCT T T --- --

Zhefu802 Zhejiang, China indica HJX74 type GAGCTGAGCT T T --- --

Zhenshan97B Jiangxi, China indica HJX74 type GAGCTGAGCT T T --- --

Wang3 Anhui, China indica HJX74 type GAGCTGAGCT T T --- --

Chaoyang1 Hunan, China indica HJX74 type GAGCTGAGCT T T --- --


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Peiai64 Hubei, China indica HJX74 type GAGCTGAGCT T T --- --

Shuhui527 Sichuan, China indica HJX74 type GAGCTGAGCT T T --- --

Gang46B Sichuan, China indica HJX74 type GAGCTGAGCT T T --- --

IR8 IRRI, Philippine indica HJX74 type GAGCTGAGCT T T --- --






IR837-36-1 IRRI, Philippine indica HJX74 type GAGCTGAGCT T T --- --

IR1544-28-2-3 IRRI, Philippine indica HJX74 type GAGCTGAGCT T T --- --

RD23 Thailand indica HJX74 type GAGCTGAGCT T T --- --

TN-1 Taiwan, China indica TN1 type GAGCTGAGCT C T --- --

Jiangxisimiao Jiangxi, China indica TN1 type GAGCTGAGCT C T --- --

Huanan15 Guangdong, China indica TN1 type GAGCTGAGCT C T --- --

Wolf-tooth D15 Vietnam indica TN1 type GAGCTGAGCT C T --- --

Amol 3 Iran indica TN1 type GAGCTGAGCT C T --- CT

19283 Egypt indica TN1 type GAGCTGAGCT C T --- --

Aus116 Bangladesh indica TN1 type GAGCTGAGCT C T --- --

Aus143 India indica TN1 type GAGCTGAGCT C T --- --

BG367-4 India indica TN1 type GAGCTGAGCT C T --- --

Wuyunjing7 Jiangsu, China japonica Basmati type ---------- C C GCG --

Xiushui11 Zhejiang, China japonica Basmati type ---------- C C GCG --

Zhonghua11 Tianjing, China japonica Basmati type ---------- C C GCG --

Dianjinyou1 Yunnan, China japonica Basmati type ---------- C C GCG --

Daohuaxiang2 Heilongjiang, China japonica Basmati type ---------- C C GCG --

Taizhong65 Taiwan, China japonica Basmati type ---------- C C GCG --

Katy USA japonica Basmati type ---------- C C GCG --

Lemont USA japonica Basmati type ---------- C C GCG --

Nipponbare Japan japonica Basmati type ---------- C C GCG --

Koshihikari Japan japonica Basmati type ---------- C C GCG --

19282 Egypt japonica Basmati type ---------- C C GCG --

Balila Italy japonica Basmati type ---------- C C GCG --

1On the basis of Garris et al (2005), five distinct variety groups were recognized for

cultivated rice: aus (AUS), indica (IND), aromatic (ARO), temperate japonica (TEJ)

and tropical japonica (TRJ).


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Supplementary Table 6 Comparisons of the physicochemical characteristics of milled rice between HJX74 and Huabiao1

Physicochemical characteristics*

HJX74 Huabiao1

GW (mm) 2.12 ± 0.01 2.00 ± 0.01

GL (mm) 5.43 ± 0.01 6.77 ± 0.02

LWR 2.56 ± 0.01 3.40 ± 0.01

GET 0.54 ± 0.01 0.72 ± 0.01

PGWC (%) 57.00 ± 0.57 39.00 ± 1.52

SGE (%) 9.15 ± 0.11 6.65 ± 0.15

AC (%) 27.27 ± 0.22 24.82 ± 0.46

GC (mm) 38.50 ± 0.50 89.00 ± 1.00

GT (ASV) 6.89 ± 0.04 6.14 ± 0.19

*GW, grain width; GL, grain length; LWR, length/width ratio; GET, grain endosperm transparency; PGWC, percentage of grains with chalkiness; SGE, square of chalky endosperm; AC, amylose content; GC, gel consistency; GT, gelatinization temperature.


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Supplementary Table 7 Primers used for the genotyping of SSSL population

Primers Chromosome Forward sequence (5'-3') Reverse sequence (5'-3')

OSR23 1 TgATACgTggTACgTgACgC TAATCgCTTCCCTACCCCTg

OSR3 1 AgCTAAggTCTgggAgAAACC AAgTAggATggggCACAAgCTC

PSM1 1 CTACTACTCCATCCgTTTC TAgCACTTCCTCCATTTC

PSM12 1 gTCAggAgACTTggTTTTgAA AggTgATgCTggAAgAATAgA

PSM13 1 CgTTggCgTAgTggACgAT gAgggTTCTTgCTTTgCTTA

PSM24 1 TgCAAgCAACAACTCCACA TCACCggCCTgAACATTTA

PSM27 1 CCCTgCACCACCgTCAACAAAg CCCACCAgCgACAACAACATCA

PSM331 1 CAgTgggCTgCgAgACAT CTgACAACgCTggTgggT

PSM354 1 ACAAgCTAAggTAgTgTCCATg CATTTTACCTCAggCTCTTCA

PSM367 1 gCACCgACAAACTAgCACAA CTgCTggTCAAATgAAggAA

PSM368 1 AgAgCAggAgAAggAAggC gCggTAATgTATTATCgCAAg

PSM369 1 gAgCCACTgATgCACCgAA CgCCACCTTCACCTTCTTgT

PSM370 1 CTCCAATCACgCCCATTT AAgCAgCCAACCATCACC

PSM371 1 TgCCTggCTAgAAggAgCT CggAgACggAggAgAACAA

PSM41 1 gAgACAAgACgAggTAACACgC gggCAgCAACAgAAgATgAATg

PSM423 1 TgCCTATTgCCTACTCACTCA TTggAggTATgTCCTTTTgCT

PSM43 1 CgTAgTggTCCATCggAggC TgAgCTgAgCTgCggCAAg

RM1 1 gCgAAAACACAATgCAAAAA gCgTTggTTggACCTgAC

RM5 1 TgCAACTTCTAgCTgCTCgA gCATCCgATCTTgATggg

RM9 1 ggTgCCATTgTCgTCCTC ACggCCCTCATCACCTTC

RM104 1 ggAAgAggAgAgAAAgATgTgTgTCg TCAACAgACACACCgCCACCgC

RM128 1 AgCTTgggTgATTTCTTggAAgCg ACgACgAggAgTCgCCgTgCAg

RM129 1 TCTCTCCggAgCCAAggCgAgg CgACgCCACgACgCgATgTACCC

RM14 1 CCgAggAgAggAgTTCgAC gTgCCAATTTCCTCgAAAAA

RM140 1 TgCCTCTTCCCTggCTCCCCTg ggCATgCCgAATgAAATgCATg

RM151 1 ggCTgCTCATCAgCTgCATgCg TCggCAgTggTAgAgTTTgATCTgC

RM212 1 CCACTTTCAgCTACTACCAg CACCCATTTgTCTCTCATTATg

RM220 1 ggAAggTAACTgTTTCCAAC gAAATgCTTCCCACATgTCT

RM226 1 AgCTAAggTCTgggAgAAACC AAgTAggATggggCACAAgCTC

RM23 1 CATTggAgTggAggCTgg gTCAggCTTCTgCCATTCTC

RM237 1 CAAATCCCgACTgCTgTCC TgggAAgAgAgCACTACAgC

RM24 1 gAAgTgTgATCACTgTAACC TACAgTggACggCgAAgTCg

RM243 1 gATCTgCAgACTgCAgTTgC AgCTgCAACgATgTTgTCC

RM246 1 gAgCTCCATCAgCCATTCAg CTgAgTgCTgCTgCgACT

RM259 1 TggAgTTTgAgAggAggg CTTgTTgCATggTgCCATgT

RM265 1 CgAgTTCgTCCAAgTgAgC CATCCACCATTCCACCAATC

RM272 1 AATTggTAgAgAggggAgAg ACATgCCATTAgAgTCAggC

RM283 1 gTCTACATgTACCCTTgTTggg CggCATgAgAgTCTgTgATg

RM294B 1 ttggcctagtgcctccaatc gagggtacaacttaggacgca


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RM297 1 TCTTTggAggCgAgCTgAg CgAAgggTACATCTgCTTAg

RM312 1 gTATgCATATTTgATAAgAg AAgTCACCgAgTTTACCTTC

RM315 1 gAggTACTTCCTCCgTTTCAC AgTCAgCTCACTgTgCAgTg

RM323 1 CAACgAgCAAATCAggTCAg gTTTTgATCCTAAggCTgCTg

RM34 1 gAAATggCAATgTgTgCg gCCggAgAACCCTAgCTC

RM35 1 TggTTAATCgATCggTCgCC CgACggCAgATATACACgg

RM403 1 gCTgTgCATgCAAgTTCATg ATggTCCTCATgTTCATggC

RM414 1 ATTgCAgTCATgCAgCAgTC ATATCTCCAATgTggCAggg

RM428 1 AACAgATggCATCgTCTTCC CgCTgCATCCACTACTgTTg

RM431 1 TCCTgCgAACTgAAgAgTTg AgAgCAAAACCCTggTTCAC

RM443 1 gATggTTTTCATCggCTACg AgTCCCAgAATgTCgTTTCg

RM446 1 ACAgCgAATACTCCAgACgg TATCTCCCCCCAAATTCCTC

RM449 1 TTgggAggTgTTgATAAggC ACCACCAgCgTCTCTCTCTC

RM462 1 ACggCCCATATAAAAgCCTC AAgATggCggAgTAgCTCAg

RM472 1 CCATggCCTgAgAgAgAgAg AgCTAAATggCCATACggTg

RM488 1 CAgCTAgggTTTTgAggCTg TAgCAACAACCAgCgTATgC

RM490 1 ATCTgCACACTgCAAACACC AgCAAgCAgTgCTTTCAgAg

RM493 1 TAgCTCCAACAggATCgACC gTACgTAAACgCggAAggTg

RM495 1 AATCCAAggTgCAgAgATgg CAACgATgACgAACACAACC

RM499 1 TACCAAACACCAACACTgCg ACCTgCAgTATCCAAgTgTACg

RM522 1 ACCAgAgAAgCCCTCCTAgC gTTCTgTggTggTCACgTTg

RM529 1 CCCTCCCTTCTgTAAgCTCC gAAgAACAATggggTTCTgg

RM543 1 CTgCTgCAgACTCTACTgCg AAATATTACCCATCCCCCCC

RM562 1 ATCAgTCggTCATAAACgCC ACCTTCCTCTTCTgCTgCTg

RM572 1 CggTTAATgTCATCTgATTgg TTCgAgATCCAAgACTgACC

RM579 1 TCCgAgTggTTATgCAAATg AATTgTgTCCAATgggCTgT

RM580 1 gATgAACTCgAATTTgCATCC CACTCCCATgTTTggCTCC

RM581 1 ACATgCgTgATCAACAATCg AATTggATgTggATgCACg

RM583 1 AgATCCATCCCTgTggAgAg gCgAACTCgCgTTgTAATC

RM84 1 TAAgggTCCATCCACAAgATg TTgCAAATgCAgCTAgAgTAC

RM86 1 TACACCTCATCgATCAATCg CTTTCgAATCTgAAgATC

OSR8 2 TCTTCTTTCTCTACTCCCTg TgCTTCCTgCCTTCCTCCA

PSM116 2 gTCCCCTCTCCTCCATTCTC CCCCCTCTCCTCACAATTC

PSM117 2 ggTTgTgCCATggCTAgTTT CgCgAgCgTTTTAgCATAAT

PSM118 2 TTCTTCCggTACTCCgTCAC gCTCgTgCgCTACTCTTCTT

PSM119 2 CAgAgAgAgAgCCCAgAggA TCTgTgCTAgCAgCCTCTCA

PSM120 2 CgAggTTAAATCggTCCTTg CgCAAggAggAgTAggAgg

PSM122 2 ACggCCCAgAgAgAgATAgC gATTAgCTATggCggAAAAgg

PSM123 2 AgggTggggAAgAAgAAgAA CTCCCTCCCTggCTTTTT

PSM124 2 gTACgTgCATgTCgTCCAAC CTCTCATggCCATCCCTAAA

PSM125 2 CCACACAAACTTggAACACg TgggTTCAAAAggACAgAgC

PSM372 2 TgAgggACTTTTggTggAA gATTAAAACCCggCCTCTAC

PSM374 2 CgTCCCAAgAgCCATAAATT TCCCCAggAAAAgAAggTg

PSM375 2 gAggAAgCAAAgCAAAgATg ggCgAgACgTggAAATAAA


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PSM376 2 gCCgCTCTTTTCCTTTTCT ACATCTTgACCATCCATCCC

RM6 2 gTCCCCTCCACCCAATTC TCgTCTACTgTTggCTgCAC

RM8 2 CACgTggCgTAAATACACgT ggCCAAACCCTAACCCTg

RM106 2 CgTCTTCATCATCgTCgCCCCg ggCCCATCCCgTCgTggATCTC

RM109 2 gCCgCCggAgAgggAgAgAgAg CCCCgACgggATCTCCATCgTC

RM110 2 TCgAAgCCATCCACCAACgAAg TCCgTACgCCgACgAggTCgAg

RM112 2 gggAggAgAggCAAgCggAgAg AgCCggTgCAgTggACggTgAC

RM138 2 AgCgCAACAACCAATCCATCCg AAgAAgCTgCCTTTgACgCTATgg

RM145 2 CCggTAggCgCCCTgCAgTTTC CAAggACCCCATCCTCggCgTC

RM154 2 ACCCTCTCCgCCTCgCCTCCTC CTCCTCCTCCTgCgACCgCTCC

RM166 2 ggTCCTgggTCAATAATTgggTTACC TTgCTgCATgATCCTAAACCgg

RM174 2 AgCgACgCCAAgACAAgTCggg TCCACgTCgATCgACACgACgg

RM183 2 ggAgCgggAgAgAgAgCCCACg TgCCgATgAAggACTgCgACgC

RM207 2 CCATTCgTgAgAAgATCTgA CACCTCATCCTCgTAACgCC

RM208 2 TCTgCAAgCCTTgTCTgATg TAAgTCgATCATTgTgTggACC

RM211 2 CCgATCTCATCAACCAACTg CTTCACgAggATCTCAAAgg

RM213 2 ATCTgTTTgCAggggACAAg AggTCTAgACgATgTCgTgA

RM221 2 ACATgTCAgCATgCCACATC TgCAAgAATCTgACCCgg

RM233A 2 CCAAATgAACCTACATgTTg gCATTgCAgACAgCTATTgA

RM236 2 gCgCTggTggAAAATgAg ggCATCCCTCTTTgATTCCTC

RM240 2 CCTTAATgggTAgTgTgCAC TgTAACCATTCCTTCCATCC

RM250 2 ggTTCAAACCAAgCTgATCA gATgAAggCCTTCCACgCAg

RM262 2 CATTCCgTCTCggCTCAACT CAgAgCAAggTggCTTgC

RM263 2 CCCAggCTAgCTCATgAACC gCTACgTTTgAgCTACCACg

RM27 2 TTTTCCTTCTCACCCACTTCA TCTTTgACAAgAggAAAgAggC

RM279 2 gCgggAgAgggATCTCCT ggCTAggAgTTAACCTCgCg

RM29 2 CAgggACCCACCTgTCATAC AACgTTggTCATATCggTgg

RM322 2 CAAgCgAAAATCCCAgCAg gATgAAACTggCATTgCCTg

RM324 2 CTgATTCCACACACTTgTgC gATTCCACgTCAggATCTTC

RM341 2 CAAgAAACCTCAATCCgAgC CTCCTCCCgATCCCAATC

RM423 2 AgCACCCATgCCTTATgTTg CCTTTTTCAgTAgCCCTCCC

RM425 2 CCAACgAAgATTCgAAgCTC CAgCACCATgAAgTCgCC

rm452 2 CTgATCgAgAgCgTTAAggg gggATCAAACCACgTTTCTg

RM475 2 CCTCACgATTTTCCTCCAAC ACggTgggATTAgACTgTgC

RM482 2 TCTgAAAgCCTgACTCATCg gTCAATTgCAgTgCCCTTTC

RM485 2 CACACTTTCCAgTCCTCTCC CATCTTCCTCTCTTCggCAC

RM492 2 CCAAAAATAgCgCgAgAgAg AAgACgTACATgggTCAggC

RM497 2 TCCTCTTCACCTATgggTgg gCCAgTgCTAggAgAgTTgg

RM498 2 AATCTgggCCTgCTCTTTTC TCCTAgggTgAAgAAAgggg

RM521 2 TTCCCTTATTCCTgCTCTCC gggATTTgCAgTgAgCTAgC

RM525 2 ggCCCgTCCAAgAAATATTg CggTgAgACAgAATCCTTACg

RM526 2 CCCAAgCAATACgTCCCTAg ACCTggTCATgACAAggAgg

RM53 2 ACgTCTCgACgCATCAATgg CACAAgAACTTCCTCggTAC

RM530 2 gCACTgACCACgACTgTTTg ACCgTAACCCggATCTATCC


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RM555 2 TTggATCAgCCAAAggAgAC CAgCATTgTggCATggATAC

RM561 2 gAgCTgTTTTggACTACggC gAgTAgCTTTCTCCCACCCC

RM599 2 CTgACCgTTgTTggTCATTg TTCCCAgAgAACCAAggATg

OSR13 3 CATTTgTgCgTCACggAgTA AgCCACAgCgCCCATCTCTC

OSR16 3 AAAACTAgCTTgCAAAggggA TgCCggCTgATCTTgTTCTC

PSM126 3 gCgAAggTAgAgCCgTAgAg ACCTCCCACCACCCTAAATC

PSM127 3 gAgggTTTAAgggggAggAg CgAgACAAAAgCCCAACAg

PSM128 3 TTgAATgCCCTCTACCAAgg TgTTggTTTTgTTgCCgATA

PSM129 3 TCCTATTCgCgTgTgACTTg ATACCgACCATCCTTgggA

PSM130 3 CTggAAACggAATTCAAACC CTCCCCTgCACCTACTACCA

PSM131 3 ggCAAggACAAAAAgAACCA AATgAggTAgCTCCTTgCCA

PSM132 3 AgTTTAgAACggggCAAgTg gCTCATCAAgACCTCAAgCA

PSM17 3 ggggATCAgAggAAgCAA AgACggAggCAAggCAAA

PSM377 3 CTCACAAgAACgCCACAAAA TgACCAgAAAggAgAAggAAA

PSM378 3 TgCTTATTggTTTgTCTTggC AAgAATAgCCTTTgCCTCCA

PSM379 3 gCATCTCCTTTTgCTgTTTCC TgCCTCgTCTCCATCAAgTTTT

PSM380 3 ACCAgTgAggCAgTgACTTgAA gCgTgCgTgTAgAAAACgAgA

PSM381 3 AACCCACTATCAgTTCACAAgC TCCCAAATTgAgTCCAgTCC

PSM428 3 gATAgAgCAgggCTggAACA ATgCTTgCCTTAgTgTCCg

PSM429 3 TATggggTggAAAggTgAA gCAggCACAAAAgCAAAgA

PSM47 3 CAgCATTATTCTggTTggCATTT ATACATCggCggAggAgCA

PSM52 3 TgCTgCgTAggAggAgAAgAgg CAAACgACCAgACATCCAACCA

RM007 3 TTCgCCATgAAgTCTCTCg CCTCCCATCATTTCgTTgTT

RM060 3 AgTCCCATgTTCCACTTCCg ATggCTACTgCCTgTACTAC

RM114 3 CAgggACgAATCgTCgCCggAg TTggCCCCCTTgAggTTgTCgg

RM130 3 TgTTgCTTgCCCTCACgCgAAg ggTCgCgTgCTTggTTTggTTC

RM132 3 ATCTTgTTgTTTCggCggCggC CATggCgAgAATgCCCACgTCC

RM135 3 CTCTgTCTCCTCCCCCgCgTCg TCAgCTTCTggCCggCCTCCTC

RM143 3 gTCCCgAACCCTAgCCCgAggg AgAggCCCTCCACATggCgACC

RM148 3 ATACAACATTAgggATgAggCTgg TCCTTAAAggTggTgCAATgCgAg

RM156 3 gCCgCACCCTCACTCCCTCCTC TCTTgCCggAgCgCTTgAggTg

RM16 3 CgCTAgggCAgCATCTAAAA AACACAgCAggTACgCgC

RM168 3 TgCTgCTTgCCTgCTTCCTTT gAAACgAATCAATCCACggC

RM175 3 CTTCggCgCCgTCATCAAggTg CgTTgAgCAgCgCgACgTTgAC

RM186 3 TCCTCCATCTCCTCCgCTCCCg gggCgTggTggCCTTCTTCgTC

RM218 3 TggTCAAACCAAggTCCTTC gACATACATTCTACCCCCgg

RM22 3 ggTTTgggAgCCCATAATCT CTgggCTTCTTTCACTCgTC

RM227 3 ACCTTTCgTCATAAAgACgAg gATTggAgAgAAAAgAAgCC

RM231 3 CCAgATTATTTCCTgAggTC CACTTgCATAgTTCTgCATTg

RM232 3 CCggTATCCTTCgATATTgC CCgACTTTTCCTCCTgACg

RM251 3 gAATggCAATggCgCTAg ATgCggTTCAAgATTCgATC

RM282 3 CTgTgTCgAAAggCTgCAC CAgTCCTgTgTTgCAgCAAg

RM293 3 TCgTTgggAggTATggTACC CTTTATCTgATCCTTgggAAgg

RM338 3 CACAggAgCAggAgAAgAgC ggCAAACCgATCACTCAgTC


12

RM347 3 CACCTCAAACTTTTAACCgCAC TCCggCAAgggATACggCgg

RM36 3 CAACTATgCACCATTgTCgC gTACTCCACAAgACCgTACC

RM3646 3 ACTAgAgCACCCTCgCTgAg CTCAgCCACCCCATCAAC

RM411 3 ACACCAACTCTTgCCTgCAT TgAAgCAAAAACATggCTAgg

RM442 3 CTTAAgCCgATgCATgAAgg ATCCTATCgACgAATgCACC

RM468 3 CCCTTCCTTgTTgTggCTAC TgATTTCTgAgAgCCAACCC

RM473D 3 TATCCTCgTCTCCATCgCTC AAggATgTggCggTAgAATg

RM489 3 ACTTgAgACgATCggACACC TCACCCATggATgTTgTCAg

RM49 3 TTCggAAgTTggTTACTgATCA TTggAgCggATTCggAgg

RM503 3 CACCTTTCACACACACACAC gCCCCACTAACAAAACCAAg

RM504 3 TCTATAATgTAgCCCCCCCC TTTCAggggCTTCTACCAAC

RM520 3 AggAgCAAgAAAAgTTCCCC gCCAATgTgTgACgCAATAg

RM545 3 CAATggCAgAgACCCAAAAg CTggCATgTAACgACAgTgg

RM546 3 gAgATgTAgACgTAgACggCg gATCATCgTCCTTCCTCTgC

RM55 3 CCgTCgCCgTAgTAgAgAAg TCCCggTTATTTTAAggCg

RM5626 3 CACAACCCACAAACAgCAAg CTTCCCCCAAAgTTTTAgCC

RM563 3 CgACCCTAgggTTTCTCC CTCgACgTCgTggAAAgC

RM565 3 AgTAACgAgCATAgCAggCg gCAAAgCCTTCAggAATCAg

RM569 3 gACATTCTCgCTTgCTCCTC TgTCCCCTCTAAAACCCTCC

RM571 3 ggAggTgAAAgCgAATCATg CCTgCTgCTCTTTCATCAgC

RM6146 3 ATCTTCTCCggTTCTCCCTC AAggAgggAggAggCAgTAg

RM85 3 CCAAAgATgAAACCTggATTg gCACAAggTgAgCAgTCC

OSR15 4 CAACCAACgCCAAAAgCTAC gTggTACTCgCCCTgCATgA

PSM103 4 CCTCCCATAAAAATCTTgTCTCTg CAACATAgTATTggATTggACACg

PSM107 4 CgAgATTAACgTCgTCCTCC CggAgATAAgACACgAgggA

PSM108 4 TAgCCATgTTgTgCTTTTgC AgAAgAggAggAgCAggAgg

PSM109 4 gTggTTgggATCggAATTg ATATCTCCTCggCTTCTCCC

PSM110 4 CCATCgCCCTTgTTgTACTT ACACAACAgTgCAACCCAAC

PSM115 4 TAgACgggATgggAAATCAC TTCgATCgATTCCAATAggC

PSM133 4 ACCgCACAAAgCATgAATTA TTggAATggAgggAgTAACg

PSM194 4 gATTCTACgggCCACAAgTC CTTgCCAAAggTCAACgAAT

PSM196 4 ggAgCCTgATgATgTgAggT TTgCCAACCCgTAAAgCTAC

PSM197 4 ggAgAAgAgCgAgTTgTTgg AgCAggAgTTgAgCTTCTCg

PSM321 4 CATgAgCCTTgCTTgCTgTA TCggTTCggTCTTATTCTgg

PSM322 4 TgTTgAgCAAATTCCAACCA AgCAgggATTggAAAggATT

PSM323 4 ACTCgTTCTgTCggTgCTCT AAAgCgggAgACAgTTgAAA

PSM326 4 AggTgCTTCCAACCATgTAAA AggTCCATTTTAACACCCCC

PSM355 4 CAAACgTACCCATCgACAAC TACgAgCACCTCCgAAgAC

PSM356 4 TCTTCATTTTgCACTCCAgAC AAACAAgAAACCAggAAgTAgC

PSM357 4 AAATgCAAATgCAgCAACg AAggAAAATggCCTCggT

PSM358 4 CTCCgTTAAgCgTgggAggA ACCgTggCCCTATCAgATgC

PSM359 4 TACgACggATCTCgCCTCT TggTTgCTTCCTCCCATAC

PSM361 4 TCCCCTgTACCTATCTgTCCC gAAgCACTTgATggCTgAAC

PSM382 4 gCAAgggACCAACCAACCT ACACgCACTCgCACTACTCg


13

PSM430 4 ggTCTAACACCCAACACCACA TgCAAAACAACCTTgCCTCT

RM119 4 CATCCCCCTgCTgCTgCTgCTg CgCCggATgTgTgggACTAgCg

RM124 4 ATCgTCTgCgTTgCggCTgCTg CATggATCACCgAgCTCCCCCC

RM127 4 gTgggATAgCTgCgTCgCgTCg AggCCAgggTgTTggCATgCTg

RM131 4 TCCTCCCTCCCTTCgCCCACTg CgATgTTCgCCATggCTgCTCC

RM142 4 CTCgCTATCgCCATCgCCATCg TCgAgCCATCgCTggATggAgg

RM177 4 CCCTCTTAgACAgAggCCAgAggg gTAgCCgAAgATgAggCCgCCg

RM185 4 AgTTgTTgggAgggAgAAAggCC AggAggCgACggCgATgTCCTC

RM241 4 gAgCCAAATAAgATCgCTgA TgCAAgCAgCAgATTTAgTg

RM252 4 TTCgCTgACgTgATAggTTg ATgACTTgATCCCgAgAACg

RM255 4 TgTTgCgTgTggAgATgTg CgAAACCgCTCAgTTCAAC

RM261 4 CTACTTCTCCCCTTgTgTCg TgTACCATCgCCAAATCTCC

RM273 4 gAAgCCgTCgTgAAgTTACC gTTTCCTACCTgATCgCgAC

RM280 4 ACACgATCCACTTTgCgC TgTgTCTTgAgCAgCCAgg

RM303 4 gCATggCCAAATATTAAAgg ggTTggAAATAgAAgTTCggT

RM317 4 CATACTTACCAgTTCACCgCC CTggAgAgTgTCAgCTAgTTgA

RM335 4 gTACACACCCACATCgAgAAg gCTCTATgCgAgTATCCATgg

RM348 4 CCgCTACTAATAgCAgAgAg ggAgCTTTgTTCTTgCgAAC

RM401 4 TggAACAgATAgggTgTAAggg CCgTTCACAACACTATACAAgC

RM417 4 CggATCCAAgAAACAgCAg TTCggTATCCTCCACACCTC

RM451 4 gATCCCCTCCgTCAAACAC CCCTTCTCCTTTCCTCAACC

RM470 4 TCCTCATCggCTTCTTCTTC AgAACCCgTTCTACgTCACg

RM471 4 ACgCACAAgCAgATgATgAg gggAgAAgACgAATgTTTgC

RM518 4 CTCTTCACTCACTCACCATgg ATCCATCTggAgCAAgCAAC

RM551 4 AgCCCAgACTAgCATgATTg gAAggCgAgAAggATCACAg

RM559 4 ACgTACACTTggCCCTATgC ATgggTgTCAgTTTgCTTCC

RM564 4 CATggCCTTgTgTATgCATC ATgCAgAggATTggCTTgAg

RM567 4 ATCAgggAAATCCTgAAggg ggAAggAgCAATCACCACTg

PSM202 5 CggAATCAATggAAggTTT CggCTAggCTATAAgTggg

PSM206 5 TggTTgggTTTggTgAT gCAgCgACTACTCCTCC

PSM208 5 CTgAggTggAgCAgggAgggT TgTTTCAgCCAgAggAgATg

PSM341 5 gACTTCgTCAACCCTCAC TTCTCCCAATCTTTCCAg

PSM362 5 ACTgCTgCTATTCCTCAAgACA ATTTgATggCAgCggTCT

PSM363 5 gACCAgTCCgTCgTAATCCA CTCCAAATgTTAATACCCTgCA

PSM383 5 gAgCgACgATgCACAATCC gCggCAAAgTTCACTgTAAg

PSM384 5 AgATgCCATgTCCAATATgCT ACTCgTgCTCTTgCTTCCA

PSM385 5 ATggAgCACgTAgTgTCgg CATggCgAggAAgATggT

PSM386 5 TgCATCCACgCAgATAgACC TgCTTggCTTggCTTgTC

PSM7 5 ggTggTgggTTAgCATCT TTggCAACTTgTAgTCTTgT

PSM8 5 CCAggTCACCACCACAAT AAATCCgAATCgCATCAg

PSM9 5 gTTgAAACCgAAACTgCC gATACAgATAATCACggAAA

RM122 5 gAgTCgATgTAATgTCATCAgTgC gAAggAggTATCgCTTTgTTggAC

RM13 5 TCCAACATggCAAgAgAgAg ggTggCATTCgATTCCAg

RM146 5 CTATTATTCCCTAACCCCCATACCCTCC AgAgCCACTgCCTgCAAggCCC


14

RM153 5 gCCTCgAgCATCATCATCAg ATCAACCTgCACTTgCCTgg

RM159 5 ggggCACTggCAAgggTgAAgg gCTTgTgCTTCTCTCTCTCTCTCTCTCTC

RM161 5 TgCAgATgAgAAgCggCgCCTC TgTgTCATCAgACggCgCTCCg

RM164 5 TCTTgCCCgTCACTgCAgATATCC gCAgCCCTAATgCTACAATTCTTC

RM169 5 TggCTggCTCCgTgggTAgCTg CAgggACgAATCgTCgCCggAg

RM173 5 CCTACCTCgCgATCCCCCCCTC CCATgAggAggAggCggCgATC

rm178 5 TCgCgTgAAAgATAAgCggCgC gATCACCgTTCCCTCCgCCTgC

RM188 5 TCCgCCTCTCCTCTCgCTTCCC gCAACgCACAACCgAACCgAgC

RM194 5 CCCTgCTTCTTgCCCACCACC TCCAgggAgggCAAggCTgAgC

RM249 5 ggCgTAAAggTTTTgCATgT ATgATgCCATgAAggTCAgC

RM26 5 gAgTCgACgAgCggCAgA CTgCgAgCgACggTAACA

RM267 5 TgCAgACATAgAgAAggAAgTg AgCAACAgCACAACTTgATg

RM274 5 CCTCgCTTATgAgAgCTTCg CTTCTCCATCACTCCCATgg

RM289 5 TTCCATggCACACAAgCC CTgTgCACgAACTTCCAAAg

RM291 5 gTTgCACTACgTATTCTgAg gATCCAgATAAATgAggCAC

RM31 5 gATCACgATCCACTggAgCT AAgTCCATTACTCTCCTCCC

RM334 5 gTTCAgTgTTCAgTgCCACC gACTTTgATCTTTggTggACg

RM405 5 TCACACACTgACAgTCTgAC AATgTggCACgTgAggTAAg

RM413 5 ggCgATTCTTggATgAAgAg TCCCCACCAATCTTgTCTTC

RM421 5 AgCTCAggTgAAACATCCAC ATCCAgAATCCATTgACCCC

RM430 5 AAACAACgACgTCCCTgATC gTgCCTCCgTggTTATgAAC

RM437 5 ACACCAACCAgATCAgggAg TgCTCgTCAATggTgAgTTC

RM440 5 CATgCAACAACgTCACCTTC ATggTTggTAggCACCAAAg

RM507 5 CTTAAgCTCCAgCCgAAATg CTCACCCTCATCATCgCC

RM509 5 TAgTgAgggAgTggAAACgg ATCgTCCCCACAATCTCATC

RM516 5 gTTTCCTgCATgCTTggAAC ATgTgATTgTATCAggCTCg

RM538 5 ggAgATgCTATAgCAgCAgTg ATTgCTCCTTACCACCTTgC

RM538 5 ggTCgTTgAAgCTTACCAgC ACAAgCTCTCAAAACTCgCC

RM548 5 TCggTgAgAAACTgAgAgTACg AAggAggCCATCTCAATgTg

RM574 5 ggCgAATTCTTTgCACTTgg ACggTTTggTAgggTgTCAC

RM592 5 TCTTTggTATgAggAACACC AgAgATCCggTTTgTTgTAA

RM593 5 TCCCgTATgTAACgTgCCA gACAAgAgAACATCgCTAgg

RM598 5 gAATCgCACACgTgATgAAC ATgCgACTgATCggTACTCC

OSR21 6 ATTTCTTTggCCACAggCgA CCCAgATTCggAACAAgAAgAAC

PMS135 6 gATgCAgCAgTgAgTTAgCg TTggggCAAAgAAgAATCTg

PMS137 6 gCCTTgCTgCCgTTTTATTA AAgCTCAACCTCAAgCATgg

PSM136 6 gATggTggCCAgggTACTTA ACTTAggggATCAggggATg

PSM138 6 ggTAggACCgTgCTTCATTg CTggCCTTTCTTCAATCTgC

PSM350 6 ACTTgAAACgAgggAggATAg gTTTCgTTgACTTgTTTTACCC

PSM387 6 TgTCgTTgAggAggTgTATgg TTgCCTTCgCACTCCAgTC

PSM388 6 AgCAAgCTCCTCCTATTCATC TTgTCTCCTCCCATCTTATCC

PSM389 6 CACCgCCTAATAATCCCC TCATTCCTCAATCCCAACAC

PSM431 6 ATgggCggCTCTTTTgTA TCACCgTCTCCACTTTTCC

RM3 6 ACACTgTAgCggCCACTg CCTCCACTgCTCCACATCTT


15

RM103 6 CTTCCAATTCAggCCggCTggC CgCCACAgCTgACCATgCATgC

RM111 6 CACAACCTTTgAgCACCgggTC ACgCCTgCAgCTTgATCACCgg

RM115 6 TTgCCgCAgTggCCgTTACCAC AggAggCggCggAAATggAAgg

RM133 6 TTggATTgTTTTgCTggCTCgC ggAACACggggTCggAAgCgAC

RM136 6 gAgAgCTCAgCTgCTgCCTCTAgC gAggAgCgCCACggTgTACgCC

RM162 6 gCCAgCAAAACCAgggATCCgg CAAggTCTTgTgCggCTTgCgg

RM170 6 TCgCgCTTCTTCCTCgTCgACg CCCgCTTgCAgAggAAgCAgCC

RM190 6 CTTTgTCTATCTCAAgACAC TTgCAgATgTTCTTCCTgATg

RM204 6 gTgAgACTgACTTggCTCATAggg gCTAgCCATgCTCTCgTACC

RM217 6 ATCgCAgCAATgCCTCgT gggTgTgAACAAAgACAC

RM225 6 TgCCCATATggTCTggATg gAAAgTggATCAggAAggC

RM253 6 TCCTTCAAgAgTgCAAAACC gCATTgTCATgTCgAAgCC

RM275 6 gCATTgATgTgCCAATCg CATTgCAACATCTTCAACATCC

RM30 6 ggTTAggCATCgTCACgg TCACCTCACCACACgACACg

RM314 6 CTAgCAggAACTCCTTTCAgg AACATTCCACACACACACgC

RM340 6 ggTAAATggACAATCCTATggC gACAAATATAAgggCAgTgTgC

RM343 6 CCACgAACCCTTTgCATC gTgATgATgCgTCggTTg

RM400 6 ACACCAggCTACCCAAACTC CggAgAgATCTgACATgTgg

RM402 6 gAgCCATggAAAgATgCATg TCAgCTggCCTATgACAATg

RM412 6 CACTTgAgAAAgTTAgTgCAgC CCCAAACACACCCAAATAC

RM439 6 TCATAACAgTCCACTCCCCC TggTACTCCATCATCCCATg

RM454 6 CTCAAgCTTAgCTgCTgCTg gTgATCAgTgCACCATAgCg

RM50 6 ACTgTACCggTCgAAgACg AAATTCCACgTCAgCCTCC

RM508 6 ggATAgATCATgTgTggggg ACCCgTgAACCACAAAgAAC

RM510 6 AACCggATTAgTTTCTCgCC TgAggACgACgAgCAgATTC

RM527 6 ggCTCgATCTAgAAAATCCg TTgCACAggTTgCgATAgAg

RM528 6 ggCATCCAATTTTACCCCTC AAATggAgCATggAggTCAC

RM539 6 gAgCgTCCTTgTTAAAACCg AgTAgggTATCACgCATCCg

RM541 6 TATAACCgACCTCAgTgCCC CCTTACTCCCATgCCATgAg

RM549 6 ACgAACTgATCATATCCgCC CTgTggTTgATCCCTgAACC

RM557 6 gTggCgAgATCTATgTggTg gCTTTgTgTgTgTgTgTgTg

RM587 6 ACgCgAACAAATTAACAgCC CTTTgCTACCAgTAgATCCAgC

RM589 6 ATCATggTCggTggCTTAAC CAggTTCCAACCAgACACTg

OSR22 7 CTgAgTCTCCTgTCCTCATC CTTgAATCTCTgCACTgCAC

PSM139 7 CgAATATggCCgACgAgT AAgCCAAgCggTgTTgATTA

PSM140 7 CCACCAACACTTAgCCgTTT CgATCgAAgTCggTCgTC

PSM141 7 gATTTgAATTgAACTggggC CAACTTCAAAgCTTCTCgCC

PSM142 7 TTTTCAgATTTCggTgACCC TTTCgggAgggATTATgACC

PSM143 7 ATTgggACACTgCATTAggC CCAgCCATTTgCCATTATgT

PSM144 7 TTTTgCTgTCCCTTTgTTCC AAgTCTTAggggATgggTgg

PSM145 7 TgTgCCTAAAATCACATggC ACCgCAgAAgTgTCTTgCTT

PSM146 7 TgACCACATCgTgCTTCTTC AgAgggTgTTCAACgAgTgg

PSM147 7 AATCATggCggATAggTgAg CgTggAACAgAAAgAAAggg

PSM148 7 ATgCgCACTATTTCAggCTT ATCCCggAgACTTCACACAT


16

PSM149 7 CTCAgATCggATTTggCATT AgAAAATTgTgCTTCCgCTg

PSM353 7 TCTgACAACTCAgTTCCAATCCCTA ggTTTgCTTCTgTTATTTgATTTCg

PSM390 7 gCATACgCATgTgCCTCTT gTgCAgTCATggCCTgATC

PSM432 7 CACTAAgCTgCTggTggCA AAAggATgggATTgggTTgT

RM2 7 ACgTgTCACCgCTTCCTC ATgTCCgggATCTCATCg

RM010 7 TTgTCAAgAggAggCATCg CAgAATgggAAATgggTCC

RM11 7 TCTCCTCTTCCCCCgATC ATAgCgggCgAggCTTAg

RM118 7 CCAATCggAgCCACCggAgAgC CACATCCTCCAgCgACgCCgAg

RM125 7 ATCAgCAgCCATggCAgCgACC AggggATCATgTgCCgAAggCC

RM172 7 TgCAgCTgCgCCACAgCCATAg CAACCACgACACCgCCgTgTTg

RM18 7 TTCCCTCTCATgAgCTCCAT gAgTgCCTggCgCTgTAC

RM182 7 TgggATgCAgAgTgCAgTTggC CgCAggCACggTgCCTTgTAAg

RM214 7 CTgATgATAgAAACCTCTTCTC AAgAACAgCTgACTTCACAA

RM234 7 ACAgTATCCAAggCCCTgg CACgTgAgACAAAgACggAg

RM248 7 TCCTTgTgAAATCTggTCCC gTAgCCTAgCATggTgCATg

RM298 7 CTgATCACTggATCgATCATg CATgCCAAgATgCAACAg

RM336 7 CTTACAgAgAAACggCATCg gCTggTTTgTTTCAggTTCg

RM418 7 TCgCgTATCgTCATgCATAg gAgCACATATgCCACgTACg

RM436 7 ATTCCTgCAgTAAAgCACgg CTTCgTgTACCTCCCCAAAC

RM47 7 ACTCCACTCCACTCCCCAC gTCAgCAggTCggACgTC

RM478 7 CAgCTggggAAgAgAgAgAg TCAgAAACTAAACgCACCCC

RM481 7 TAgCTAgCCgATTgAATggC CTCCACCTCCTATgTTgTTg

RM500 7 gAgCTTgCCAgAgTggAAAg gTTACACCgAgAgCCAgCTC

RM505 7 AgAgTTATgAgCCgggTgTg gATTTggCgATCTTAgCAgC

RM51 7 TCTCgATTCAATgTCCTCgg CTACgTCATCATCgTCTTCCC

RM533 7 gCAACTgCTCTACgCCTCTC CCTgAggCTTCACCTACTCg

RM560 7 gCAggAggAACAgAATCAgC AgCCCgTgATACggTgATAg

RM70 7 gTggACTTCATTTCAACTCg gATgTATAAgATAgTCCC

RM82 7 TgCTTCTTgTCAATTCgCC CgACTCgTggAggTACgg

OSR30 8 TCACCgTCgAATCgAATCCA AgTCgAggAAggAgAAgTTC

OSR7 8 AACTCATTTgTCACACgCACA AAgCCTTTCCTCgTAACACg

PSM150 8 CTgAgTCCCCATgATCACCT CAACTAACCCAgTCgCCATT

PSM151 8 gCCgCCATAgTATTCCTTCC ACACCCTACCAAACgAgCTg

PSM152 8 CCCCgTATACCCggATTATT TggTCgTggACAgTgCTCTA

PSM153 8 TggCCAATTCTTCTTCTgCT gCggACgCgTAAAAAgATTA

PSM154 8 CATTgggTTTgTgCATTCAg CAACgACCCATATTCCAACC

PSM155 8 ggggTgACTTgAggTAgTgg gCgACTTCAgTTCTCCACCT

PSM352 8 gCAAgCTCgCAACTACCA TAATCAAgCATTCgCTgTCT

PSM393 8 TCCCAgCCCATCATCTTCC AAAATCAAATggCgACAgC

PSM394 8 TgTTggATTTgTTCTTTACCgC CACAACAAgCTCgACgTgATC

PSM395 8 gACAAAgAAgAgCAAgAAAgCC TggAAgAATCCTCCCCAAgA

PSM396 8 CCCTggACCTTgAgCgTTAg CCgCTTCTTCCTCCAAACC

RM38 8 ACgAgCTCTCgATCAgCCTA TCggTCTCCATgTCCCAC

RM137 8 gACATCgCCACCAgCCCACCAC CgggTggTCCCCgAggATCTTg


17

RM149 8 gCTgACCAACgAACCTAggCCg gTTggAAgCCTTTCCTCgTAACACg

RM152 8 gAAACCACCACACCTCACCg CCgTAgACCTTCTTgAAgTAg

RM195 8 AgAAAgAgAggCCgTCggCggC gggCTCACCCCCAAACCTgCAg

RM210 8 TCACATTCggTggCATTg CgAggATggTTgTTCACTTg

RM223 8 gAgTgAgCTTgggCTgAAAC gAggCAAgTCTTggCACTg

RM230 8 gCCAgACCgTggATgTTC CACCgCAgTCACTTTTCAAg

RM25 8 ggAAAgAATgATCTTTTCATgg CTACCATCAAAACCAATgTTC

RM256 8 gACAgggAgTgATTgAAggC gTTgATTTCgCCAAgggC

RM264 8 gTTgCgTCCTACTgCTACTTC gATCCgTgTCgATgATTAgC

RM281 8 ACCAAgCATCCAgTgACCAg gTTCTTCATACAgTCCACATg

RM284 8 ATCTCTgATACTCCATCCATCC CCTgTACgTTgATCCgAAgC

RM308 8 ggCTgCACACgCACACTATA TTACgCATATggTgAgTAggC

RM337 8 gTAggAAAggAAgggCAgAg CgATAgATAgCTAgATgTggCC

RM339 8 gTAATCgATgCTgTgggAAg gAgTCATgTgATAgCCgATATg

RM344 8 CAgAgACAATAgTCCCTgCAC gTAggAggAgATggATgATgg

RM404 8 CCAATCATTAACCCCTgAgC gCCTTCATgCTTCAgAAgAC

RM407 8 gATTgAggAgACgAgCCATC CTTTTTCAgATCTgCgCTCC

RM42 8 ATCCTACCgCTgACCATgAg TTTggTCTACgTggCgTACA

RM433 8 TgCgCTgAACTAAACACAgC AgACAAACCTggCCATTCAC

RM44 8 ACgggCAATCCgAACAACC TCgggAAAACCTACCCTACC

RM447 8 CCCTTgTgCTgTCTCCTCTC ACgggCTTCTTCTCCTTCTC

RM502 8 gCgATCgATggCTACgAC ACAACCCAACAAgAAggACg

RM506 8 CgAgCTAACTTCCgTTCTgg gCTACTTgggTAgCTgACCg

RM515 8 TAggACgACCAAAgggTgAg TggCCTgCTCTCTCTCTCTC

RM547 8 TAggTTggCAgACCTTTTCg gTCAAgATCATCCTCgTAgCg

RM556 8 ACTCCAAACCTCACTgCACC TAgCACACTgAACAgCTggC

RM72 8 CCggCgATAAAACAATgAg gCATCggTCCTAACTAAggg

RM80 8 TTgAAggCgCTgAAggAg CATCAACCTCgTCTTCACCg

OSR28 9 AgCAgCTATAgCTTAgCTgg ACTgCACATgAgCAgAgACA

PSM156 9 CTCACgCgAgggTTTACTgT ggAggAAACAgCAgCAACTC

PSM157 9 gCggTTTAggAgCgTTTgTA ggAgggAgTACgTgACATgg

PSM158 9 AgTgAgTgACCggCCTATTg TgCAAgAACTTTggggTAgC

PSM159 9 TgCAgATggAggACgAggA CTgCATgCTTCgTTCAgCTA

PSM160 9 gATggATgATgggAgAATgg TCATgCCAACTTTTgACCAC

PSM161 9 CAATAgggCATgTCAggTCA TgCCCATTgTCCTCTAgCTT

PSM337 9 AAgAAACAgCgCCCAATg ggAgCCgAAAgCgTAgAg

PSM340 9 CTCTgTATCCgCgAgTCCAC CCgggTTATAgAACAAgCACA

PSM397 9 AAAgCCATgTAACCATTCAgCC ggAAggAAATTCAgCTCCgTTT

PSM399 9 TAATgCCTTACCATAACTTCgC TACCATgCAACATgCTCCC

PSM400 9 CTgCTTgTggTCgTCTCgT CTTCACCAACAATCTACATCgA

PSM404 9 ACAAgAACgTAggCgAgAAAA AgTgAgTggggTggAggAgA

RM105 9 gTCgTCgACCCATCggAgCCAC TgTCgAggTggggATCgggTC

RM160 9 AgCTAgCAgCTATAgCTTAgCTggAgATC TCTCATCgCCATgCgAggCCTC

RM201 9 CTCgTTTATTACCTACAgTACC CTACCTCCTTTCTAgACCgATA


18

RM205 9 CTggTTCTgTATgggAgCAg CTggCCCTTCACgTTTCAgTg

RM215 9 CAAAATggAgCAgCAAgAgC TgAgCACCTCCTTCTCTgTAg

RM219 9 CgTCggATgATgTAAAgCCT CATATCggCATTCgCCTg

RM242 9 ggCCAACgTgTgTATgTCTC TATATgCCAAgACggATggg

RM245 9 ATgCCgCCAgTgAATAgC CTgAgAATCCAATTATCTgggg

RM257 9 CAgTTCCgAgCAAgAgTACTC ggATCggACgTggCATATg

RM278 9 gTAgTgAgCCTAACAATAATC TCAACTCAgCATCTCTgTCC

RM285 9 CTgTgggCCCAATATgTCAC ggCggTgACATggAgAAAg

RM288 9 CCggTCAgTTCAAgCTCTg ACgTACggACgTgACgAC

RM321 9 CCAACACTgCCACTCTgTTC gAggATggACACCTTgATCg

RM328 9 CATAgTggAgTATgCAgCTgC CCTTCTCCCAgTCgTATCTg

RM409 9 CCgTCTCTTgCTAgggATTC ggggTgTTTTgCTTTCTCTg

RM410 9 gCTCAACgTTTCgTTCCTg gAAgATgCgTAAAgTgAACgg

RM434 9 gCCTCATCCCTCTAACCCTC CAAgAAAgATCAgTgCgTgg

RM444 9 gCTCCACCTgCTTAAgCATC TgAAgACCATgTTCTgCAgg

RM460 9 TgATCgACAgCgTTCTTgAC gCCTggCCCACATAATTAAg

RM464 9 AACgggCACATTCTgTCTTC TggAAgACCTgATCgTTTCC

RM524 9 TgAAgAgCAggAACCgTAgg TCTgATATCggTTCCTTCgg

RM566 9 ACCCAACTACgATCAgCTCg CTCCAggAACACgCTCTTTC

PSM162 10 gCCACATCTCTTgggTgAgA CATCTgCAggAggCgTAgTC

PSM163 10 gCgCACTTggAgAAAgAAAg CCCACCTCTCAgTgCgATTA

PSM164 10 ACCAAATTggCTgCTCCTTA CCAgCCACTggTAgTCACAA

PSM165 10 TTggCTTgTCgAAAggATCT ATTgCCgAAgATggAggAg

PSM166 10 gCCATgAgAAgATgAAACTgC CTCCATTTTCTCCCCAAACA

PSM167 10 ACgAAATCCAACgCATCAAg gCAACACTgCCAAACCCTAT

PSM168 10 AgCTAgCCATTggCATCATC CCACCgAAAAgTCgACgATA

PSM169 10 CCCCCTgAACTACAAAACCA gCCgCCgTCTAAAggAgA

PSM170 10 gACCAACTCCTCCTCCACAg AgATCAgAgAggCCggATg

PSM405 10 CgCTCATCCCTACCCTCTgT gATTTgCgATgCgACAgC

PSM406 10 gACCgTTCgATCTgCCATCA ggCgACTTAggAgCgTTTgT

PSM407 10 CggCCACTAATAgTgACCgA TgCgATgAgAATACACCCAAA

RM147 10 TACggCTTCggCggCTgATTCC CCCCCgAATCCCATCgAAACCC

RM184 10 ATCCCATTCgCCAAAACCggCC TgACACTTggAgAgCggTgTgg

RM216 10 gCATggCCgATggTAAAg TgTATAAAACCACACggCCA

RM222 10 CTTAAATgggCCACATgCg CAAAgCTTCCggCCAAAAg

RM228 10 CTggCCATTAgTCCTTgg gCTTgCggCTCTgCTTAC

RM239 10 TACAAAATgCTgggTACCCC ACATATgggACCCACCTgTC

RM244 10 CCgACTgTTCgTCCTTATCA CTgCTCTCgggTgAACgT

RM258 10 TgCTgTATgTAgCTCgCACC TggCCTTTAAAgCTgTCgC

RM269 10 gAAAgCgATCgAACCAgC gCAAATgCgCCTCgTgTC

RM271 10 TCAgATCTACAATTCCATCC TCggTgAgACCTAgAgAgCC

RM304 10 TCAAACCggCACATATAAgAC gATAgggAgCTgAAggAgATg

RM311 10 TggTAgTATAggTACTAAACAT TCCTATACACATACAAACATAC

RM333 10 gTACgACTACgAgTgTCACCAA gTCTTCgCgATCACTCgC


19

RM467 10 ggTCTCTCTCTCTCTCTCTCTCTC CTCCTgACAATTCAACTgCg

RM474 10 AAgATgTACgggTggCATTC TATgAgCTggTgAgCAATgg

RM496 10 gACATgCgAACAACgACATC gCTgCggCgCTgTTATAC

RM590 10 CATCTCCgCTCTCCATgC ggAgTTggggTCTTgTTCg

RM591 10 CTAgCTAgCTggCACCAgTg TggAgTCCgTgTTgTAgTCg

RM596 10 ATCTACACggACgAATTgCC AgAAgCTTCAgCCTCTgCAg

RM330A 11 CAATgAAgTggATCTCggAg CATCAATCAgCgAAggTCC

OSR1 11 ACCATggATggTACCAACTC TTCgCTTgCATCTACTATgC

PMS171 11 CCTTTCCCTCAACCAAATCC CATTCTCggTgCACTCCTC

PSM172 11 CgCCTgCAAAAATCATACCT ggTCggATTAggAgggAgAg

PSM173 11 TCCTTgCCTTCTgCAAACTT AAAgTgCATggTgCAgATCA

PSM174 11 gAgATggCTAAAgCgAgCAg gCgACCACCAgTAggAACAg

PSM175 11 ggTgAATgTgTTgCTgTTgg CCATCACCCAAATCCAAgAA

PSM176 11 ggTgATgCATgCTTgATCTg gACgAACACACAAggggAAT

PSM177 11 gCCAgAggAAACAACAAggA AggCCgAgAAgTCgAAgTTT

PSM346 11 TAAAgCTCTgCACTgAgCCA ggCTTgAgAgCgTTTgTAgg

PSM365 11 ggTgggAACTAATCCATCgT TTCggCAgCCAACTTTTC

PSM366 11 AAAAgCAAAgAAAgggTgAgTC ggTggAAATCgAAAATgAggT

PSM408 11 CTTgCTTCgCTTgCATCTACT CCTTCATCCTCgTCCCCTA

PSM409 11 TATgACggCTTAggAgTgTTTg CggggATTTTCAgAATAgCg

PSM410 11 TACCCAACAAgTAggCATTgAA CgTggAgCTTTACCCATTTTAT

PSM412 11 gggTgAgTATggCAAgAgCAC AgCCCCAACAATCCgAAAA

PSM413 11 ATTCCTTTAgCCCACCTCTTTA AgTggATgggATCTTggTTTAT

PSM414 11 CTgTAggCTgTACCTCCACg TTgCgAgTTAgAgggATTAgTT

PSM415 11 CTCCCTCCTgCTCgTTTTCTC CACCTAgTTAggTAgCgCCCAT

PSM416 11 CCAgAgCCCCgAATCATCA TCTCCgCCCCAAACCCTAA

PSM417 11 gCTCAATACTTgTCCACCCg TTCgTTTCgCTTCCCTCAC

PSM418 11 gTCCgTCggggAACCAgATA TggAAAggggAAAgggAgg

RM120 11 CACACAAgCCCTgTCTCACgACC CgCTgCgTCATgAgTATgTA

RM144 11 TgCCCTggCgCAAATTTgATCC gCTAgAggAgATCAgATggTAgTgCATg

RM167 11 gATCCAgCgTgAggAACACgT AgTCCgACCACAAggTgCgTTgTC

RM202 11 CAgATTggAgATgAAgTCCTCC CCAgCAAgCATgTCAATgTA

RM206 11 CCCATgCgTTTAACTATTCT CgTTCCATCgATCCgTATgg

RM209 11 ATATgAgTTgCTgTCgTgCg CAACTTgCATCCTCCCCTCC

RM20B 11 ATCTTgTCCCTgCAggTCAT gAAACAgAggCACATTTCATTg

RM21 11 ACAgTATTCCgTAggCACgg gCTCCATgAgggTggTAgAg

RM224 11 ATCgATCgATCTTCACgAgg TgCTATAAAAggCATTCggg

RM229 11 CACTCACACgAACgACTgAC CgCAggTTCTTgTgAAATgT

RM254 11 AgCCCCgAATAAATCCACCT CTggAggAgCATTTggTAgC

RM286 11 ggCTTCATCTTTggCgAC CCggATTCACgAgATAAACTC

RM287 11 TTCCCTgTTAAgAgAgAAATC gTgTATTTggTgAAAgCAAC

RM332 11 gCgAAggCgAAggTgAAg CATgAgTgATCTCACTCACCC

RM441 11 ACACCAgAgAgAgAgAgAgAgAg TCTgCAACggCTgATAgATg

RM457 11 CTCCAgCATggCCTTTCTAC ACCTgATggTCAAAgATggg


20

RM479 11 CCCCTTgCTAgCTTTTggTC CCATACCTCTTCTCCTCCCC

RM536 11 TCTCTCCTCTTgTTTggCTC ACACACCAACACgACCACAC

OSR20 12 TggTCAAgTgACTTAggTgg AgAgCTCCAACTCTTTACAAg

PSM178 12 gCgTTgTggAgTgACAgAgA gTgTTgCCCAAggAATTgAg

PSM181 12 CgAAgTCggTCATCAATggT TCgATCCCATCTTCATCTCC

PSM182 12 CCTTgATCTTgATCCCTCCA TgTCTTCCTCCTTggTTTgg

PSM183 12 gACAAAACCggCAACAgATT TAgCTTTATTgTggCAgggg

PSM185 12 TTACAggAgggAATggATgC gAAgTgggCgATACggAAg

PSM186 12 ggCCTCACCTgATgTgTCTT CTCCgAAATTTCCAACCTgA

PSM187 12 AATACCgATgAACAgCggAg TCAACTAggAAggTAgCCCg

PSM188 12 CCTTCTTCTTCACggCAgAg gCgAggTTAgCAAgACTggA

PSM189 12 TgATAgTTggACCCgATCCT ATCACCCCTCTgCTCgTCT

PSM190 12 CACgTCTCTAgTTTTTCCAgAgC TCggCAgTgTATCAAggTgA

PSM191 12 gCgAggAgAAgCTTAAgACg AgACggAAAACgATgAgCAC

PSM192 12 CTgCAgCCCTgTgATAggAT TgTgCggTTgCTTTCATAgA

PSM193 12 CATCgTCCTCggTATCATCA gTgAAACggAgggAgTAgCA

PSM402 12 gATACCTggATgTTgCg gAggAggTCTTAgTTTggA

PSM419 12 gAggCAATAggAggggAgA ggCAgCgAAAgCATAAAgTA

PSM420 12 ACCTATggCTTgCgACgAAC CTgAgTTgACAATATCATCACggA

PSM421 12 TTggCgTgCTgCTgTATTTT gTCCCggATTCATggTTCTg

RM004A 12 TTgACgAggTCAgCACTgAC AgggTgTATCCgACTCATCg

RM101 12 gTgAATggTCAAgTgACTTAggTggC ACACAACATgTTCCCTCCCATgC

RM17 12 TgCCCTgTTATTTTCTTCTCTC ggTgATCCTTTCCCATTTCA

RM179 12 CCCCATTAgTCCACTCCACCACC CCAATCAgCCTCATgCCTCCCC

RM19 12 CAAAAACAgAgCAgATgAC CTCAAgATggACgCCAAgA

RM20A 12 ATCTTgTCCCTgCAggTCAT gAAACAgAggCACATTTCATTg

RM235 12 AgAAgCTAgggCTAACgAAC TCACCTggTCAgCCTCTTTC

RM247 12 TAgTgCCgATCgATgTAACg CATATggTTTTgACAAAgCg

RM260 12 ACTCCACTATgACCCAgAg gAACAATCCCTTCTACgATCg

RM270 12 ggCCgTTggTTCTAAAATC TgCgCAgTATCATCggCgAg

RM277 12 CggTCAAATCATCACCTgAC CAAggCTTgCAAgggAAg

RM309 12 gTAgATCACgCACCTTTCTgg AgAAggCCTCCggTgAAg

RM313 12 TgCTACAAgTgTTCTTCAggAC gCTCACCTTTTgTgTTCCAC

RM415 12 CTTCgATCCATCATCCATgg ATTgCTgTACgCAgTTTCgg

RM453 12 CgCATCTCTCTCCCTTATCg CTCTCCTCCTCgTTgTCgTC

RM463 12 TTCCCCTCCTTTTATggTgC TgTTCTCCTCAgTCACTgCg

RM491 12 ACATgATgCgTAgCgAgTTg CTCTCCCTTCCCAATTCCTC

RM511 12 CTTCgATCCggTgACgAC AACgAAAgCgAAgCTgTCTC

RM512 12 CTgCCTTTCTTACCCCCTTC AACCCCTCgCTggATTCTAg

RM519 12 AgAgAgCCCCTAAATTTCCg AggTACgCTCACCTgTggAC


21

Supplementary Table 8 Primers used for fine mapping and sequencing

Primers Forward sequence (5'-3') Reverse sequence (5'-3')

OSR7 AACTCATTTgTCACACgCACA AAgCCTTTCCTCgTAACACg

PSM127 gAgggTTTAAgggggAggAg CgAgACAAAAgCCCAACAg

PSM143 ATTgggACACTgCATTAggC CCAgCCATTTgCCATTATgT

PSM168 AgCTAgCCATTggCATCATC CCACCgAAAAgTCgACgATA

PSM695 CATCgCTTCCAATgCCCTCC CCCTCTTCTCCgTTCTgACg

PSM699 CTCAgATggAACTgAAggC CAACTggCTAATATggTgCT

PSM709 TCACTgATTCCCTTTTACgTTT gCTCAgTTgCTTCTTCATTAgA

PSM710 gCCAgCCAAgAAAAgCgACA TCTTgAgATCCCACTCCATg

PSM711 ATgACCgTCTgCTTCCTCTAA AACATCgACAgggAgAAgTgC

PSM712 TTCAgATTgggTATgCTCAT CATTCAgACTTTCAgAggCA

PSM733 gCCATAgTTATCgTCgTTT ggAgTATTTgTTTgTTTCg

PSM734 gAgACgATgTgACTgAAAAg ATgAATCTgAACAAAggTATg

PSM736 AAAggCgAgTCgTggTAAAgA gATggAgATggTgAgTgggTg

RM14 CCgAggAgAggAgTTCgAC gTgCCAATTTCCTCgAAAAA

RM147 TACggCTTCggCggCTgATTCC CCCCCgAATCCCATCgAAACCC

RM172 TgCAgCTgCgCCACAgCCATAg CAACCACgACACCgCCgTgTTg

RM18 TTCCCTCTCATgAgCTCCAT gAgTgCCTggCgCTgTAC

RM212 CCACTTTCAgCTACTACCAg CACCCATTTgTCTCTCATTATg

RM239 TACAAAATgCTgggTACCCC ACATATgggACCCACCTgTC

RM251 gAATggCAATggCgCTAg ATgCggTTCAAgATTCgATC

RM258 TgCTgTATgTAgCTCgCACC TggCCTTTAAAgCTgTCgC

RM273 gAAgCCgTCgTgAAgTTACC gTTTCCTACCTgATCgCgAC

RM280 ACACgATCCACTTTgCgC TgTgTCTTgAgCAgCCAgg

RM281 ACCAAgCATCCAgTgACCAg gTTCTTCATACAgTCCACATg

RM282 CTgTgTCgAAAggCTgCAC CAgTCCTgTgTTgCAgCAAg

RM293 TCgTTgggAggTATggTACC CTTTATCTgATCCTTgggAAgg

RM294b TTggCCTAgTgCCTCCAATC gagggTaCaaCTTaggaCgCa

RM323 CAACgAgCAAATCAggTCAg gTTTTgATCCTAAggCTgCTg

RM335 gTACACACCCACATCgAgAAg gCTCTATgCgAgTATCCATgg

RM336 CTTACAgAgAAACggCATCg gCTggTTTgTTTCAggTTCg

RM3754 TCgTAggTggggCTAACAAg CACCCTCTTCTTCCTCCgAC

RM401 TggAACAgATAgggTgTAAggg CCgTTCACAACACTATACAAgC

RM447 CCCTTgTgCTgTCTCCTCTC ACgggCTTCTTCTCCTTCTC

RM462 ACggCCCATATAAAAgCCTC AAgATggCggAgTAgCTCAg

RM467 ggTCTCTCTCTCTCTCTCTCTCTC CTCCTgACAATTCAACTgCg

RM470 TCCTCATCggCTTCTTCTTC AgAACCCgTTCTACgTCACg

RM471 ACgCACAAgCAgATgATgAg gggAgAAgACgAATgTTTgC

RM474 AAgATgTACgggTggCATTC TATgAgCTggTgAgCAATgg

RM481 TAgCTAgCCgATTgAATggC CTCCACCTCCTATgTTgTTg

RM488 CAgCTAgggTTTTgAggCTg TAgCAACAACCAgCgTATgC


22

RM502 gCgATCgATggCTACgAC ACAACCCAACAAgAAggACg

RM505 AgAgTTATgAgCCgggTgTg gATTTggCgATCTTAgCAgC

RM51 TCTCgATTCAATgTCCTCgg CTACgTCATCATCgTCTTCCC

RM545 CAATggCAgAgACCCAAAAg CTggCATgTAACgACAgTgg

RM5493 gACAAAACACAAAgCAggAC TAACAAACCAACCAACCAAg

RM55 CCgTCgCCgTAgTAgAgAAg TCCCggTTATTTTAAggCg

RM551 AgCCCAgACTAgCATgATTg gAAggCgAgAAggATCACAg

RM562 CACAACCCACAAACAgCAAg CTTCCCCCAAAgTTTTAgCC

RM569 gACATTCTCgCTTgCTCCTC TgTCCCCTCTAAAACCCTCC

RM580 gATgAACTCgAATTTgCATCC CACTCCCATgTTTggCTCC

RM590 CATCTCCgCTCTCCATgC ggAgTTggggTCTTgTTCg

RM80 TTgAAggCgCTgAAggAg CATCAACCTCgTCTTCACCg

reg9 gATggCTACgACgTCACT TTACATTTgCTgggTTTg

reg8 TTgggTTgTTggTAgTATgg CAgggATgATTTCAgTAggA

reg7-3 TgTAggCTCCAAgCATCATC CCATATCCCACAgCATCTCC

reg7-2 gAATTggATAgCgATTggAC ATgAgTgCTTCCTCCgTTTC

reg6-2 ACACCTCACgTACCCTACAC gCCCTTCCATgCgATCTATC

reg5 gCTAgTggTACAgTTTggTCg AgCTCAgTTgCTTCTTCATTAgA

reg4 TggTCTgATTTTTCACTgATTCC gTgAAggTCATCTTTCCTgCTg

reg3 ggAAAgTTTgCTgATgTCggT TTgTTTAAgCTATgAgTTgggAg

regf2 CgCTTgTTTggCTggTCgTT CTTCTTCTTCTgATgAATgCgT

regf1 TCTCAggCAgAAAggTggTTAg ggAAAgTAggAgTAgTggCAAAgAT

pro-2 CAAgggAAgCAgAAACCCAgCAA gCAgCCgATgACgACgATACCg

enin01 TggTggCAggCAggAgT gCgggAAggAAggAgACAAT

exon1-3 gACAgCCACggAATCgAgAgT gTCTTggggTgTTTTgggCTA

exon2 CTggATTgggTTTCAAgCAT CTgCgATATCATAgAgTggT

exon3-1 CACAACCgTCgCCgCAggAAgC CgCCgTCAgAggTggAgCCAAC

exon3-2 ggTCgTCTAgCCCATCACCTTT gCAgTTCTCACTTggCACCATCA

intr01 TTTgCTCgCCTTTAgATT TgTTCAgggCTCCATTTT

intr02 CAgATAgCCAgggTgCTCT ACggTTgTgCCCATCTAgTC

link gATgCCCAAACCTAgAAAgAT TCACCCTATgATACTCAAACgA

poly1 AATgAgATgAACTACAATgggATg gTTCCAgCgAggCTCAAgAT

3-utr1 ACTACAATgggATgTTTCACg TCTgTTCCAAACgATgCCTAAg

3-utr2 CAACATgATggTgCCAAgTgAg TAggAAAAAgTTgCTCCACAT

3-utr2-2 CCAAAgTAgATgTTgggATAgAAg gCTgAAgCCTgAAAgTATgTg

3-utr3 AAAATACggACgACgAAATTAC AAAAggATggAgATggTgAgTg


23

Supplementary Table 9 Primers used for DNA constructs and transcripts analysis

Primer Sequence (5'-3')

pGW8-EcoRIF CggAATTCgTCgTTgTTCAATTgTCTTgT pGW8-KpnIR ggggTACCgCTCgCTgTAgCTCAgCTTA pGW8-BamHI ggCCggATCCgCTTgTTTggCTggTCgTT pGW8-XbaI TCCCCCCggggCTCgCTgTAgCTCAgCTTA GW8-KpnIF ggggTACCATggAgTgggATCTCAAgATgC GW8-BamHIR CgggATCCCTACTgCCATgAgAACggCAgA GW8-F-EcoRI CCggAATTCATggAgTgggATCTCAAgATg GW8-R2-EcoRI CCggAATTCTACTgCCATgAgAACggCAg GW8-RNAiR1 gCTCTAgA AAgCTgATCTCgCCTTCCTgg GW8-RNAiF CgggATCCATggAgTgggATCTCAAgATg GW8-RNAiR2 gCTCTAgACggCgCCgACgACgCTgCTgCT N1-R-BamHI CgCggATCCTTACTgCTgCTgCCCCgCC C1-F-EcoRI CCggAATTCATgTgCCCgTCgTgCgCgg N2-R-BamHI CgCggATCCCTACTgTggCTTCCTgCgg C2-F-EcoRI CCggAATTCATggATCCCATgAACTCTgC Zinc-F-EcoRI CCggAATTCATgTgCCCgTCgTgCgCggTg Zinc-R-BamHI CgCggATCCCTAATCTggCTgTggCTTCC qrt-Actin3F CCACTATgTTCCCTggCATT qrt-ActinR gTACTCAgCCTTggCAATCC gw8-qrt-2f AggAgTTTgATgAggCCAAg gw8-qrt-2r gCgTgTAgTATgggCTCTCC RNAi-realtimeF gCTCCAATgACAATgAgATgA RNAi-realtimeR CTgAAACTAAggCAgCAACA GW2-RT-F CAgCAgCgCATTCCCAgTTTTC GW2-RT-R gTggTCAgCCgAgCACTCTC GS3-RT-F CATCggAgAAgCgAAgTCAT GS3-RT-R TTgAggTTgAAggAggAggA GS5-RT-F CATTCCATgCAAATgCCAgTggAC GS5-RT-R CAgCCCTgCTTTgATgAgCTTg DEP1F CCgTTTCTCgTTCTggAT DEP1R ATCTgTgCCTCCTTCTCT SPL14-1F CAgCTACCACATgAAgTC SPL14-1R CTACAgAgACCAATCCATC FZPF CTCCAgCATgTCgTCgTC FZPR CACCACgCTgCTCAggTA GN1AF gATAgCCTACAAgCAgTA GN1AR gCCTTTggATCATACTTg LAX1F gATgACgACgCTggAgAT LAX1R gACATTgCACACCgAgTAg OsCLV1F ACATgCTCTCCAACCCAAACTCg


24

OsCLV1R TAgCCgCTAATTAAgCgCACAC OsCLV2F CgAACAACCgAATATCTg OsCLV2R gAACgAgAACATCCAATC OsCLV3F AgACgAATggCTAgTATT OsCLV3R CTCTCATCCgATTCCTAC RCN1F gACCTgCgATCTTTCTTCAC RCN1R gACAATTggAgCTgCATTTC RCN2F CgATCTTgCATggACAAAAC RCN2R CTAggACTCTCCTgCCATgg Atactin2-F catcctccgtcttgaccttgc Atactin2-R acgattcctggacctgcctc AGL24F gAggCTTTggAgACAgAgTCggTgA AGL24R AgATggAAgCCAAgCTTCAgggAA AGL42-F TCATgAAACCAgCAATCACgACTCA AGL42-R AgCCTTTCTTTCTCggACCTTTCC AP1-F AgggAAAAAATTCTTAgggCTCAACAg AP1-R gCggCgAAgCAgCCAAggTTgCAgTTg CAL-F AgCTCAggAgATCTCTgTTCTTTgTgATg CAL-R ggCTTgATgATgTTgATATgATATAAggAC CO-F TCCATggAAACTggTgTTgTgC CO-R TTgCAgggTCAggTTgTTgCTC FT-F gAAgAACCTTTggCAATgAgA FT-R AgCCACTCTCCCTCTgACAA FUL-RT-F gAgAgggAgAAgAAAACgggTCAg FUL-RT-R CTCTCCCCCAACTCTCTCCAC LFY-RT-F gAgAACgTTggCTCATggCgTC LFY-RT-R CTCCAAATggCAAAgCTgACgCAg SOC1F TgAggCATACTAAggATCgAg SOC1R gCgTCTCTACTTCAgAACTTgggC


25

Supplementary Figure 1. Semidominant effect of GW8 on grain width and shape

(a) Segregation of the F2 population derived from the cross between the selected

SSSL (W09-38-60-7-7) and HJX74 plant. Comparisons of (b) grain length, (c) grain

width, (d) length/width ratio among homozygotes of the Basmati385 qgw8 allele,

heterozygotes of the Basmati385 qgw8 and the HJX74 qGW8 allele, and homozygotes

of the HJX74 qGW8 allele. Data represent mean ± SE (n=160).


26

Supplementary Figure 2. Homology analysis of OsSPL16 protein sequence

The GW8/OsSPL16 protein sequence aligned with its homologous SPL proteins

among maize, rice, sorghum, barley and Arabidopsis. The numbers on the right

indicate the position of the residues in the full protein. Identical residues indicated by

dark boxes, conserved residues indicated by grey boxes and variant residues by light

boxes. The sequences of HvGW8 (BAK05794.1) and SbGW8 (XP_002445815.1) are

from NCBI (http://www.ncbi.nlm.nih.gov/).


27

Supplementary Figure 3. The expression of OsSPL16 in the transgenic plants

The transcriptional levels of OsSPL16 were determined by qRT-PCR using young

panicles of length 6cm. (a) The independent transgenic NIL-GW8 lines carrying a

pActin::RNAi-OsSPL16 construct. The Transgenic line R6 formed grain which was

shown in Fig. 2a. (b) The independent transgenic NIL-gw8 lines expressing HJX74

OsSPL16 cDNA. The Transgenic line H48 formed grain which was shown in Fig. 2b.

(c) The independent transgenic NIL-gw8 lines expressing Basmati385 OsSPL16

cDNA. The Transgenic line T36 formed grain which was shown in Fig. 2c. (d) The

independent transgenic Basmati385 lines expressing Basmati385 OsSPL16 cDNA.

The Transgenic line B68 formed grain which was shown in Fig. 2a. The values were

expressed relative to the level of transcripts in NIL-GW8, NIL-gw8 and Basmati385,

respectively, set to be one. All data given as mean ± SE (n=4).


28

Supplementary Figure 4. The effect of OsSPL16 on grain size and shape

(a) Comparisons of grain length. (b) Comparisons of grain width. (c) Comparisons of

the ratio for the length to width. Data given as mean ± SE (n=60). A Student’s t-test

was used to generate the P values.


29

Supplementary Figure 5. GUS expression driven by the native OsSPL16

promoter

(a) In transgenic plants carrying the pOsSPL16::GUS construct showed no evidence of

any GUS expression in the root, culm, leaf blade and shoot meristem and young

panicles in the early stage of inflorescence development, whereas GUS activity could

be detected in panicles of length 3-16cm. (b) There was strong GUS expression in the

stamen and spikelet hulls during spikelet development. Scale bar: 2 mm.


30

Supplementary Figure 6. Histological analysis of spikelet hulls

(a) Close-up view of longitudinal sections of spikelet hull. Scale bar, 25μm. (b)

Comparison of cell length of inner parenchyma cell layer between NIL-GW8 and

NIL-gw8 plants. Data given as mean ± SE (n=12). A Student’s t-test was used to

generate the P values.


31

Supplementary Figure 7. Subcellular localization and transcription activity

analysis of OsSPL16 protein

(a) Scheme of OsSPL16 showing the site of the SBP-domain and Gly rich region.

Numbers represent amino acid positions in OsSPL16 (1 indicates the start Met, and

454 indicates the final Trp). (b) The OsSPL16-GFP fusion protein is present in the

nucleus. Scale bar, 10μm. (c) Transcription activity assay. The full-length cDNA of

OsSPL16 from HJX74 and DNA fragments responsible for different truncated

deletions were introduced into the pGBKT7 vector. DNA-BD represents GAL4 DNA

binding domain. The empty pGBKT7was the negative control.


32

Supplementary Figure 8. Comparisons of transcripts of the genes for the

determination of grain size between NIL-GW8 and NIL-gw8 plants

The analysis of relative expression ratios was determined by qRT-PCR using young

panicles during the inflorescence development. (a) Young panicle (<0.2cm in length).

(b) Panicle of length 3cm. (c) Panicle of length 6cm. (d) Panicle of length 9cm. The

values were expressed relative to the level of transcripts in NIL-GW8 set to be one.


33

Supplementary Figure 9. Effect of OsSPL16 on the expression of genes involved

in cell cycle

The analysis of relative expression ratios was determined by qRT-PCR using 7-8 cm

young panicles. The values were expressed relative to the level of transcript in

NIL-GW8 set to be one.


34

Supplementary Figure 10. OsSPL16 promotes cell proliferation and plant growth

in Arabidopsis

(a) The contrasting phenotype of 7-d-old seedlings. Scale bar: 5mm. (b) Cotyledon

area. Scale bar: 0.5mm. (c) SEM-images of cotyledon pavement cells. Scale bar:

50μm. (d) Size of cotyledon pavement cells. Data given as mean ± SE (n=15). (e, f)

Root meristem size. Scale bar: 50μm. White arrowheads indicate cortex transition

zone. Data given as mean ± SE (n=40). A Student’s t-test was used to generate the P

values.


35

Supplementary Figure 11. Effect of OsSPL16 on flowering time

(a) Phenotypic characterization of transgenic Arabidopsis plants carrying a

pUbi::OsSPL16 construct. Scale bar: 1cm. (b-c) Effect of increased OsSPL16 activity

on flowering time. Comparison of rosette leaf number in b and chronological time in c.

Data given as mean ± SE (n=40). A Student’s t-test was used to generate the P values.

(d) The analysis of expression ratios was determined by qRT-PCR using 10-day-old

seedlings grown in long day condition. Arabidopsis actin2 was used as a control. The

values were expressed relative to the level of transcripts in wild type set to be one.


36

Supplementary Figure 12. Phenotype of transgenic rice plants constitutively

expressing OsSPL16

(a) Transgenic plants overexpressing OsSPL16 were dwarfed, and produced fewer

panicle branches. Scale bar: 10cm. (b) The abnormal glume architectures in transgenic

plants. Scale bar: 2.5mm. The contrast between transgenic HJX74 plants and

non-transgenic control plants with respect to (c) the number of primary branches per

panicle, (d) the number of secondary branches per panicle, (e) the number of grains

per panicle, (f) the length of the uppermost internode. (g) Cross-sections of the

uppermost internodes. Scale bar: 5mm. (h) Longitudinal sections of the uppermost

internodes of a mature plant. Scale bar: 50μm. (i) Comparison of cell length of

longitudinal sections of the uppermost internodes. Data given as mean ± SE (n=12).


37

Supplementary Figure 13. Effect of OsSPL16 on endosperm size and grain filling

(a) Representative endosperms after fertilization at 3 days, 6 days, 9 days, 12 days and

21 days, respectively. Scale bar: 2mm. (b-c) Time-course of endosperm fresh and dry

weight increase. Data given as mean ± SE (n=20).


38

Supplementary Figure 14. Effect of OsSPL16 on starch granule in endosperm

Comparison of scanning election microscopy images of the transverse sections of

starch granule between NIL-GW8 and NIL-gw8 plants. NIL-gw8 endosperm was

filled with compactly arranged polygonal granules with sharp edges.


39

9311 ATGGAGTGGGATCTCAAGATGCCGCCGGCGGCGAGTTGGGAGCTAGCCGACGAGCTGGAG

HJX74 ATGGAGTGGGATCTCAAGATGCCGCCGGCGGCGAGTTGGGAGCTAGCCGACGAGCTGGAG

MH63 ATGGAGTGGGATCTCAAGATGCCGCCGGCGGCGAGTTGGGAGCTAGCCGACGAGCTGGAG

Basmati385 ATGGAGTGGGATCTCAAGATGCCGCCGGCGGCGAGCTGGGAGCTAGCCGACGAGCTGGAG

Basmati370 ATGGAGTGGGATCTCAAGATGCCGCCGGCGGCGAGCTGGGAGCTAGCCGACGAGCTGGAG

Amol ATGGAGTGGGATCTCAAGATGCCGCCGGCGGCGAGCTGGGAGCTAGCCGACGAGCTGGAG

JXSM ATGGAGTGGGATCTCAAGATGCCGCCGGCGGCGAGCTGGGAGCTAGCCGACGAGCTGGAG

LJ33 ATGGAGTGGGATCTCAAGATGCCGCCGGCGGCGAGTTGGGAGCTAGCCGACGAGCTGGAG

9311 AACAGCGGCGGCGGGGGTGTACCGGCGGCGGTATCGTCGTCATCGGCTGCGGTTGGTGGC

HJX74 AACAGCGGCGGCGGGGGTGTACCGGCGGCGGTATCGTCGTCATCGGCTGCGGTTGGTGGC

MH63 AACAGCGGCGGCGGGGGTGTACCGGCGGCGGTATCGTCGTCATCGGCTGCGGTTGGTGGC

Basmati385 AACAGCGGCGGCGGGGGTGTACCGGCGGCGGTATCGTCGTCATCGGCTGCGGTTGGTGGC

Basmati370 AACAGCGGCGGCGGGGGTGTACCGGCGGCGGTATCGTCGTCATCGGCTGCGGTTGGTGGC

Amol AACAGCGGCGGCGGGGGTGTACCGGCGGCGGTATCGTCGTCATCGGCTGCGGTTGGTGGC

JXSM AACAGCGGCGGCGGGGGTGTACCGGCGGCGGTATCGTCGTCATCGGCTGCGGTTGGTGGC

LJ33 AACAGCGGCGGCGGGGGTGTACCGGCGGCGGTATCGTCGTCATCGGCTGCGGTTGGTGGC

9311 GGCGTCAATGCGGGGGGTGGTGGCAGGCAGGAGTGCTCGGTCGACCTCAAGCTCGGCGGG

HJX74 GGCGTCAATGCGGGGGGTGGTGGCAGGCAGGAGTGCTCGGTCGACCTCAAGCTCGGCGGG

MH63 GGCGTCAATGCGGGGGGTGGTGGCAGGCAGGAGTGCTCGGTCGACCTCAAGCTCGGCGGG

Basmati385 GGCGTCAATGCGGGGGGTGGTGGCAGGCAGGAGTGCTCGGTCGACCTCAAGCTCGGCGGG

Basmati370 GGCGTCAATGCGGGGGGTGGTGGCAGGCAGGAGTGCTCGGTCGACCTCAAGCTCGGCGGG

Amol GGCGTCAATGCGGGGGGTGGTGGCAGGCAGGAGTGCTCGGTCGACCTCAAGCTCGGCGGG

JXSM GGCGTCAATGCGGGGGGTGGTGGCAGGCAGGAGTGCTCGGTCGACCTCAAGCTCGGCGGG

LJ33 GGCGTCAATGCGGGGGGTGGTGGCAGGCAGGAGTGCTCGGTCGACCTCAAGCTCGGCGGG

9311 TTGGGGGAGTTCGGCGGCGGCGGCGCGCAGCCGCGGGTCGCCGTGGCGGGCGAGCTGGCC

HJX74 TTGGGGGAGTTCGGCGGCGGCGGCGCGCAGCCGCGGGTCGCCGTGGCGGGCGAGCTGGCC

MH63 TTGGGGGAGTTCGGCGGCGGCGGCGCGCAGCCGCGGGTCGCCGTGGCGGGCGAGCTGGCC

Basmati385 TTGGGGGAGTTCGGCGGCGGCGGCGCGCAGCCGCGGGTCGCCGTGGCGGGCGAGCCGGCC

Basmati370 TTGGGGGAGTTCGGCGGCGGCGGCGCGCAGCCGCGGGTCGCCGTGGCGGGCGAGCCGGCC

Amol TTGGGGGAGTTCGGCGGCGGCGGCGCGCAGCCGCGGGTCGCCGTGGCGGGCGAGCTGGCC

JXSM TTGGGGGAGTTCGGCGGCGGCGGCGCGCAGCCGCGGGTCGCCGTGGCGGGCGAGCTGGCC

LJ33 TTGGGGGAGTTCGGCGGCGGCGGCGCGCAGCCGCGGGTCGCCGTGGCGGGCGAGCTGGCC

9311 AAGGGGAAGGGGCCAGCGGCCGCCGCCACGGGAGCAGCAGCAGCAGCGTCGTCGGCGCCG

HJX74 AAGGGGAAGGGGCCAGCGGCCGCCGCCACGGGAGCAGCAGCAGCAGCGTCGTCGGCGCCG

MH63 AAGGGGAAGGGGCCAGCGGCCGCCGCCACGGGAGCAGCAGCAGCAGCGTCGTCGGCGCCG

Basmati385 AAGGGGAAGGGGCCAGCGGCCGCCGCCACGGGAGCAGCAGCAGCAGCGTCGTCGGCGCCG

Basmati370 AAGGGGAAGGGGCCAGCGGCCGCCGCCACGGGAGCAGCAGCAGCAGCGTCGTCGGCGCCG

Amol AAGGGGAAGGGGCCAGCGGCCGCCGCCACGGGAGCAGCAGCAGCAGCGTCGTCGGCGCCG

JXSM AAGGGGAAGGGGCCAGCGGCCGCCGCCACGGGAGCAGCAGCAGCAGCGTCGTCGGCGCCG

LJ33 AAGGGGAAGGGGCCAGCGGCCGCCGCCACGGGAGCAGCAGCAGCAGCGTCGTCGGCGCCG


40

9311 GCGAAGCGGCCGCGCGGTGCGGCGGCGG---GGCAGCAGCAGTGCCCGTCGTGCGCGGTG

HJX74 GCGAAGCGGCCGCGCGGTGCGGCGGCGG---GGCAGCAGCAGTGCCCGTCGTGCGCGGTG

MH63 GCGAAGCGGCCGCGCGGTGCGGCGGCGG---GGCAGCAGCAGTGCCCGTCGTGCGCGGTG

Basmati385 GCGAAGCGGCCGCGCGGTGCGGCGGCGGCGGGGCAGCAGCAGTGCCCGTCGTGCGCGGTG

Basmati370 GCGAAGCGGCCGCGCGGTGCGGCGGCGGCGGGGCAGCAGCAGTGCCCGTCGTGCGCGGTG

Amol GCGAAGCGGCCGCGCGGTGCGGCGGCGG---GGCAGCAGCAGTGCCCGTCGTGCGCGGTG

JXSM GCGAAGCGGCCGCGCGGTGCGGCGGCGG---GGCAGCAGCAGTGCCCGTCGTGCGCGGTG

LJ33 GCGAAGCGGCCGCGCGGTGCGGCGGCGG---GGCAGCAGCAGTGCCCGTCGTGCGCGGTG

9311 GACGGGTGCAAGGAGGACCTGAGCAAGTGCCGCGACTACCATCGCCGGCACAAGGTGTGC

HJX74 GACGGGTGCAAGGAGGACCTGAGCAAGTGCCGCGACTACCATCGCCGGCACAAGGTGTGC

MH63 GACGGGTGCAAGGAGGACCTGAGCAAGTGCCGCGACTACCATCGCCGGCACAAGGTGTGC

Basmati385 GACGGGTGCAAGGAGGACCTGAGCAAGTGCCGCGACTACCATCGCCGGCACAAGGTGTGC

Basmati370 GACGGGTGCAAGGAGGACCTGAGCAAGTGCCGCGACTACCATCGCCGGCACAAGGTGTGC

Amol GACGGGTGCAAGGAGGACCTGAGCAAGTGCCGCGACTACCATCGCCGGCACAAGGTGTGC

JXSM GACGGGTGCAAGGAGGACCTGAGCAAGTGCCGCGACTACCATCGCCGGCACAAGGTGTGC

LJ33 GACGGGTGCAAGGAGGACCTGAGCAAGTGCCGCGACTACCATCGCCGGCACAAGGTGTGC

9311 GAGGCCCACTCCAAGACCCCCCTCGTCGTCGTCTCCGGCCGCGAGATGCGCTTCTGCCAG

HJX74 GAGGCCCACTCCAAGACCCCCCTCGTCGTCGTCTCCGGCCGCGAGATGCGCTTCTGCCAG

MH63 GAGGCCCACTCCAAGACCCCCCTCGTCGTCGTCTCCGGCCGCGAGATGCGCTTCTGCCAG

Basmati385 GAGGCCCACTCCAAGACCCCCCTCGTCGTCGTCTCCGGCCGCGAGATGCGCTTCTGCCAG

Basmati370 GAGGCCCACTCCAAGACCCCCCTCGTCGTCGTCTCCGGCCGCGAGATGCGCTTCTGCCAG

Amol GAGGCCCACTCCAAGACCCCCCTCGTCGTCGTCTCCGGCCGCGAGATGCGCTTCTGCCAG

JXSM GAGGCCCACTCCAAGACCCCCCTCGTCGTCGTCTCCGGCCGCGAGATGCGCTTCTGCCAG

LJ33 GAGGCCCACTCCAAGACCCCCCTCGTCGTCGTCTCCGGCCGCGAGATGCGCTTCTGCCAG

9311 CAGTGCAGCAGGTTTCACTTGCTTCAGGAGTTTGATGAGGCCAAGCGCAGCTGTAGAAAG

HJX74 CAGTGCAGCAGGTTTCACTTGCTTCAGGAGTTTGATGAGGCCAAGCGCAGCTGTAGAAAG

MH63 CAGTGCAGCAGGTTTCACTTGCTTCAGGAGTTTGATGAGGCCAAGCGCAGCTGTAGAAAG

Basmati385 CAGTGCAGCAGGTTTCACTTGCTTCAGGAGTTTGATGAGGCCAAGCGCAGCTGTAGAAAG

Basmati370 CAGTGCAGCAGGTTTCACTTGCTTCAGGAGTTTGATGAGGCCAAGCGCAGCTGTAGAAAG

Amol CAGTGCAGCAGGTTTCACTTGCTTCAGGAGTTTGATGAGGCCAAGCGCAGCTGTAGAAAG

JXSM CAGTGCAGCAGGTTTCACTTGCTTCAGGAGTTTGATGAGGCCAAGCGCAGCTGTAGAAAG

LJ33 CAGTGCAGCAGGTTTCACTTGCTTCAGGAGTTTGATGAGGCCAAGCGCAGCTGTAGAAAG

9311 CGACTAGATGGGCACAACCGTCGCCGCAGGAAGCCACAGCCAGATCCCATGAACTCTGCA

HJX74 CGACTAGATGGGCACAACCGTCGCCGCAGGAAGCCACAGCCAGATCCCATGAACTCTGCA

MH63 CGACTAGATGGGCACAACCGTCGCCGCAGGAAGCCACAGCCAGATCCCATGAACTCTGCA

Basmati385 CGACTAGATGGGCACAACCGTCGCCGCAGGAAGCCACAGCCAGATCCCATGAACTCTGCA

Basmati370 CGACTAGATGGGCACAACCGTCGCCGCAGGAAGCCACAGCCAGATCCCATGAACTCTGCA

Amol CGACTAGATGGGCACAACCGTCGCCGCAGGAAGCCACAGCCAGATCCCATGAACTCTGCA

JXSM CGACTAGATGGGCACAACCGTCGCCGCAGGAAGCCACAGCCAGATCCCATGAACTCTGCA


41

LJ33 CGACTAGATGGGCACAACCGTCGCCGCAGGAAGCCACAGCCAGATCCCATGAACTCTGCA

9311 AGTTATCTTGCAAGCCAACAAGGGGCAAGATTCTCACCGTTCGCGACGCCGAGACCGGAG

HJX74 AGTTATCTTGCAAGCCAACAAGGGGCAAGATTCTCACCGTTCGCGACGCCGAGACCGGAG

MH63 AGTTATCTTGCAAGCCAACAAGGGGCAAGATTCTCACCGTTCGCGACGCCGAGACCGGAG

Basmati385 AGTTATCTTGCAAGCCAACAAGGGGCAAGATTCTCACCGTTCGCGACGCCGAGACCGGAG

Basmati370 AGTTATCTTGCAAGCCAACAAGGGGCAAGATTCTCACCGTTCGCGACGCCGAGACCGGAG

Amol AGTTATCTTGCAAGCCAACAAGGGGCAAGATTCTCACCGTTCGCGACGCCGAGACCGGAG

JXSM AGTTATCTTGCAAGCCAACAAGGGGCAAGATTCTCACCGTTCGCGACGCCGAGACCGGAG

LJ33 AGTTATCTTGCAAGCCAACAAGGGGCAAGATTCTCACCGTTCGCGACGCCGAGACCGGAG

9311 GCAAGCTGGACAGGGATGATCAAAACCGAGGAGAGCCCATACTACACGCACCACCAAATC

HJX74 GCAAGCTGGACAGGGATGATCAAAACCGAGGAGAGCCCATACTACACGCACCACCAAATC

MH63 GCAAGCTGGACAGGGATGATCAAAACCGAGGAGAGCCCATACTACACGCACCACCAAATC

Basmati385 GCAAGCTGGACAGGGATGATCAAAACCGAGGAGAGCCCATACTACACGCACCACCAAATC

Basmati370 GCAAGCTGGACAGGGATGATCAAAACCGAGGAGAGCCCATACTACACGCACCACCAAATC

Amol GCAAGCTGGACAGGGATGATCAAAACCGAGGAGAGCCCATACTACACGCACCACCAAATC

JXSM GCAAGCTGGACAGGGATGATCAAAACCGAGGAGAGCCCATACTACACGCACCACCAAATC

LJ33 GCAAGCTGGACAGGGATGATCAAAACCGAGGAGAGCCCATACTACACGCACCACCAAATC

9311 CCTCTTGGCATCAGCAGCAGGCAGCAGCATTTCGTTGGCTCCACCTCTGACGGCGGCCGC

HJX74 CCTCTTGGCATCAGCAGCAGGCAGCAGCATTTCGTTGGCTCCACCTCTGACGGCGGCCGC

MH63 CCTCTTGGCATCAGCAGCAGGCAGCAGCATTTCGTTGGCTCCACCTCTGACGGCGGCCGC

Basmati385 CCTCTTGGCATCAGCAGCAGGCAGCAGCATTTCGTTGGCTCCACCTCTGACGGCGGCCGC

Basmati370 CCTCTTGGCATCAGCAGCAGGCAGCAGCATTTCGTTGGCTCCACCTCTGACGGCGGCCGC

Amol CCTCTTGGCATCAGCAGCAGGCAGCAGCATTTCGTTGGCTCCACCTCTGACGGCGGCCGC

JXSM CCTCTTGGCATCAGCAGCAGGCAGCAGCATTTCGTTGGCTCCACCTCTGACGGCGGCCGC

LJ33 CCTCTTGGCATCAGCAGCAGGCAGCAGCATTTCGTTGGCTCCACCTCTGACGGCGGCCGC

9311 CGCTTCCCTTTCCTCCAGGAAGGCGAGATCAGCTTCGGCAACGGCGCCGGCGCCGGCGGC

HJX74 CGCTTCCCTTTCCTCCAGGAAGGCGAGATCAGCTTCGGCAACGGCGCCGGCGCCGGCGGC

MH63 CGCTTCCCTTTCCTCCAGGAAGGCGAGATCAGCTTCGGCACCGGCGCCGGCGCCGGCGGC

Basmati385 CGCTTCCCTTTCCTCCAGGAAGGCGAGATCAGCTTCGGCACCGGCGCCGGCGCCGGCGGC

Basmati370 CGCTTCCCTTTCCTCCAGGAAGGCGAGATCAGCTTCGGCACCGGCGCCGGCGCCGGCGGC

Amol CGCTTCCCTTTCCTCCAGGAAGGCGAGATCAGCTTCGGCACCGGCGCCGGCGCCGGCGGC

JXSM CGCTTCCCTTTCCTCCAGGAAGGCGAGATCAGCTTCGGCACCGGCGCCGGCGCCGGCGGC

LJ33 CGCTTCCCTTTCCTCCAGGAAGGCGAGATCAGCTTCGGCACCGGCGCCGGCGCCGGCGGC

9311 GTGCCAATGGATCAGGCAGCAGCTGCTGCTGCTGCTTCAGTGTGCCAGCCACTTCTGAAG

HJX74 GTGCCAATGGATCAGGCAGCAGCTGCTGCTGCTGCTTCAGTGTGCCAGCCACTTCTGAAG

MH63 GTGCCAATGGATCAGGCAGCAGCTGCTGCTGCTGCTTCAGTGTGCCAGCCACTTCTGAAG

Basmati385 GTGCCAATGGATCAGGCAGCAGCTGCTGCTGCTGCTTCAGTGTGCCAGCCACTTCTGAAG

Basmati370 GTGCCAATGGATCAGGCAGCAGCTGCTGCTGCTGCTTCAGTGTGCCAGCCACTTCTGAAG


42

Amol GTGCCAATGGATCAGGCAGCAGCTGCTGCTGCTGCTTCAGTGTGCCAGCCACTTCTGAAG

JXSM GTGCCAATGGATCAGGCAGCAGCTGCTGCTGCTGCTTCAGTGTGCCAGCCACTTCTGAAG

LJ33 GTGCCAATGGATCAGGCAGCAGCTGCTGCTGCTGCTTCAGTGTGCCAGCCACTTCTGAAG

9311 ACGGTAGCTCCTCCTCCTCCTCCTCATGGCGGCGGCGGCAGCGGCGGCGGCAAGATGTTC

HJX74 ACGGTAGCTCCTCCTCCTCCTCCTCATGGCGGCGGCGGCAGCGGCGGCGGCAAGATGTTC

MH63 ACGGTAGCTCCTCCTCCTCCTCCTCATGGCGGCGGCGGCAGCAGCGGCGGCAAGATGTTC

Basmati385 ACGGTAGCTCCTCCTCCTCCTCCTCATGGCGGCGGCGGCAGCGGCGGCGGCAAGATGTTC

Basmati370 ACGGTAGCTCCTCCTCCTCCTCCTCATGGCGGCGGCGGCAGCGGCGGCGGCAAGATGTTC

Amol ACGGTAGCTCCTCCTCCTCCTCCTCATGGCGGCGGCGGCAGCGGCGGCGGCAAGATGTTC

JXSM ACGGTAGCTCCTCCTCCTCCTCCTCATGGCGGCGGCGGCAGCGGCGGCGGCAAGATGTTC

LJ33 ACGGTAGCTCCTCCTCCTCCTCCTCATGGCGGCGGCGGCAGCGGCGGCGGCAAGATGTTC

9311 TCCGATGGTGGGTTGACACAAGTGCTCGACTCCGATTGTGCTCTCTCTC--TTCTGTCAG

HJX74 TCCGATGGTGGGTTGACACAAGTGCTCGACTCCGATTGTGCTCTCTCTC--TTCTGTCAG

MH63 TCCGATGGTGGGTTGACACAAGTGCTCGACTCCGATTGTGCTCTCTCTC--TTCTGTCAG

Basmati385 TCCGATGGTGGGTTGACACAAGTGCTCGACTCCGATTGTGCTCTCTCTC--TTCTGTCAG

Basmati370 TCCGATGGTGGGTTGACACAAGTGCTCGACTCCGATTGTGCTCTCTCTC--TTCTGTCAG

Amol TCCGATGGTGGGTTGACACAAGTGCTCGACTCCGATTGTGCTCTCTCTCTCTTCTGTCAG

JXSM TCCGATGGTGGGTTGACACAAGTGCTCGACTCCGATTGTGCTCTCTCTC--TTCTGTCAG

LJ33 TCCGATGGTGGGTTGACACAAGTGCTCGACTCCGATTGTGCTCTCTCTC--TTCTGTCAG

9311 CTCCGGCGAACTCCACGGCCATCGACGTCGGCGGTGGCCGGGTGGTCGTCCAGCCGACCG

HJX74 CTCCGGCGAACTCCACGGCCATCGACGTCGGCGGTGGCCGGGTGGTCGTCCAGCCGACCG

MH63 CTCCGGCGAACTCCACGGCCATCGACGTCGGCGGTGGCCGGGTGGTCGTCCAGCCGACCG

Basmati385 CTCCGGCGAACTCCACGGCCATCGACGTCGGCGGTGGCCGGGTGGTCGTCCAGCCGACCG

Basmati370 CTCCGGCGAACTCCACGGCCATCGACGTCGGCGGTGGCCGGGTGGTCGTCCAGCCGACCG

Amol CTCCGGCGAACTCCACGGCCATCGACGTCGGCGGTGGCCGGGTGGTCGTCCAGCCGACCG

JXSM CTCCGGCGAACTCCACGGCCATCGACGTCGGCGGTGGCCGGGTGGTCGTCCAGCCGACCG

LJ33 CTCCGGCGAACTCCACGGCCATCGACGTCGGCGGTGGCCGGGTGGTCGTCCAGCCGACCG

9311 AGCACATCCCCATTGCGCAGCCTCTCATCTCTGGCCTTCAGTTCGGCGGCGGCGGCGGCA

HJX74 AGCACATCCCCATTGCGCAGCCTCTCATCTCTGGCCTTCAGTTCGGCGGCGGCGGCGGCA

MH63 AGCACATCCCCATTGCGCAGCCTCTCATCTCTGGCCTTCAGTTCGGCGGCGGCGGCGGCA

Basmati385 AGCACATCCCCATGGCGCAGCCTCTCATCTCTGGCCTTCAGTTCGGCGGCGGCGGCGGCA

Basmati370 AGCACATCCCCATGGCGCAGCCTCTCATCTCTGGCCTTCAGTTCGGCGGCGGCGGCGGCA

Amol AGCACATCCCCATTGCGCAGCCTCTCATCTCTGGCCTTCAATTCGGCGGCGGCGGCGGCA

JXSM AGCACATCCCCATTGCGCAGCCTCTCATCTCTGGCCTTCAATTCGGCGGCGGCGGCGGCA

LJ33 AGCACATCCCCATTGCGCAGCCTCTCATCTCTGGCCTTCAATTCGGCGGCGGCGGCGGCA

9311 GCTCAGCCTGGTTCGCGGCGCGGCCGCATCATCAGGCGGCCACCGGCGCCGCCGCCACCG

HJX74 GCTCAGCCTGGTTCGCGGCGCGGCCGCATCATCAGGCGGCCACCGGCGCCGCCGCCACCG

MH63 GCTCAGCCTGGTTCGCGGCGCGGCCGCATCATCAGGCGGCCACCGGCGCCGCCGCCACCG

Basmati385 GCTCAGCCTGGTTCGCGGCGCGGCCGCATCATCAGGCGGCCACCGGCGCCGCCGCCACCG


43

Basmati370 GCTCAGCCTGGTTCGCGGCGCGGCCGCATCATCAGGCGGCCACCGGCGCCGCCGCCACCG

Amol GCTCAGCCTGGTTCGCGGCGCGGCCGCATCATCAGGCGGCCACCGGCGCCACCGCCACCG

JXSM GCTCAGCCTGGTTCGCGGCGCGGCCGCATCATCAGGCGGCCACCGGCGCCACCGCCACCG

LJ33 GCTCAGCCTGGTTCGCGGCGCGGCCGCATCATCAGGCGGCCACCGGCGCCACCGCCACCG

9311 CCGTCGTCGTCTCGACGGCCGGTTTCTCCTGCCCGGTGGTGGAGAGCGAGCAGCTGAACA

HJX74 CCGTCGTCGTCTCGACGGCCGGTTTCTCCTGCCCGGTGGTGGAGAGCGAGCAGCTGAACA

MH63 CCGTCGTCGTCTCGACGGCCGGTTTCTCCTGCCCGGTGGTGGAGAGCGAGCAGCTGAACA

Basmati385 CCGTCGTCGTCTCGACGGCCGGTTTCTCCTGCCCGGTGGTGGAGAGCGAGCAGCTGAACA

Basmati370 CCGTCGTCGTCTCGACGGCCGGTTTCTCCTGCCCGGTGGTGGAGAGCGAGCAGCTGAACA

Amol CCGTCGTCGTCTCGACGGCCGGTTTCTCCTGCCCGGTGGTGGAGAGCGAGCAGCTGAACA

JXSM CCGTCGTCGTCTCGACGGCCGGTTTCTCCTGCCCGGTGGTGGAGAGCGAGCAGCTGAACA

LJ33 CCGTCGTCGTCTCGACGGCCGGTTTCTCCTGCCCGGTGGTGGAGAGCGAGCAGCTGAACA

9311 CAGTCCTGAGCTCCAATGACAATGAGATGAACTACAATGGGATGTTTCACGTCGGCGGCG

HJX74 CAGTCCTGAGCTCCAATGACAATGAGATGAACTACAATGGGATGTTTCACGTCGGCGGCG

MH63 CAGTCCTGAGCTCCAATGACAATGAGATGAACTACAATGGGATGTTTCACGTCGGCGGCG

Basmati385 CAGTCCTGAGCTCCAATGACAATGAGATGAACTACAATGGGATGTTTCACGTCGGCGGCG

Basmati370 CAGTCCTGAGCTCCAATGACAATGAGATGAACTACAATGGGATGTTTCACGTCGGCGGCG

Amol CAGTCCTGAGCTCCAATGACAATGAGATGAACTACAATGGGATGTTTCACGTCGGCGGCG

JXSM CAGTCCTGAGCTCCAATGACAATGAGATGAACTACAATGGGATGTTTCACGTCGGCGGCG

LJ33 CAGTCCTGAGCTCCAATGACAATGAGATGAACTACAATGGGATGTTTCACGTCGGCGGCG

9311 AAGGCTCATCGGATGGCACGTCGTCGTCTCTGCCGTTCTCATGGCAGTAG

HJX74 AAGGCTCATCGGATGGCACGTCGTCGTCTCTGCCGTTCTCATGGCAGTAG

MH63 AAGGCTCATCGGATGGCACGTCGTCGTCTCTGCCGTTCTCATGGCAGTAG

Basmati385 AAGGCTCATCGGATGGCACGTCGTCGTCTCTGCCGTTCTCATGGCAGTAG

Basmati370 AAGGCTCATCGGATGGCACGTCGTCGTCTCTGCCGTTCTCATGGCAGTAG

Amol AAGGCTCATCGGATGGCACGTCGTCGTCTCTGCCGTTCTCATGGCAGTAG

JXSM AAGGCTCATCGGATGGCACGTCGTCGTCTCTGCCGTTCTCATGGCAGTAG

LJ33 AAGGCTCATCGGATGGCACGTCGTCGTCTCTGCCGTTCTCATGGCAGTAG

Supplementary Figure 15. Allelic variations in the coding region of OsSPL16

The detailed information of cultivated varieties was listed in Supplemental Table 2.

Variant nucleotides were in the red and blue color.


44

Supplementary Figure 16. Comparison of grain yield between Huabiao1 and

HJX74 plants

Yield test in paddies between Huabiao1 and HJX74 plants were performed under

normal cultivation conditions at Experimental Stations of South China Agricultural

University. A randomized complete block experimental design with three replications

was performed in 2009, 2010 and 2011, respectively. Data given as mean ± SE (n=3),

each plot contains 200 plants. A Student’s t-test was used to generate the P values.


45

Supplementary Figure 17. Comparisons of transcripts of genes determining

panicle branching between NIL-GW8 and NIL-gw8Amol plants

The analysis of relative expression ratios was determined by qRT-PCR using young

panicles at the early stage of inflorescence development. The values were expressed

relative to the level of transcript in NIL-GW8 set to be one.


Documents

Supplementary Information Supplementary tables · PDF fileqGW8/qgw8 165 2.46 ± 0.01 8.53 ± 0.02 3.47 ± 0.01 ... Nature Genetics: doi:10.1038/ng.2327. 2 Supplementary Table 3 Yield