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Sympathetic denervation in the mice ~ My life in San Diego. 生科 04 楊慧儒 891644 2002.12.06. 劉新光 教授 John H. K. Liu, Ph.D.. the regulation of intraocular pressure An elevated intraocular pressure ─ primary open-angle glaucoma - PowerPoint PPT Presentation
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Sympathetic denervation in the mice
~ My life in San Diego
生科 04 楊慧儒 891644
2002.12.06
劉新光 教授 John H. K. Liu, Ph.D.
the regulation of intraocular pressure An elevated intraocular pressure ─
primary open-angle glaucoma the circadian (24-hour) rhythms of intraocular pr
essure and related ocular functions. Human study─ eye drop for glaucoma
Summer research
Superior cervical ganglionectomy Way to measure the pupil size of the mice Test of eye drop on postsurgical mice
Superior Cervical Ganglion (SCG)
Superior Cervical Ganglion (SCG)
Sympathetic nerve─change in pupillary reflex Removal or sectioning of this part can reduce th
e circadian IOP elevation The outcome of removal of SCG or cutting the c
ervical sympathetic trunk will mimic human Horner’s syndrome─drug test
PAST~ Palpebral ptosis and become irreversible from 20th hr Palpebral retraction
Pharmacology of the sympathetic nervous system
hydroxyamphetamine
Drug to Test Horner’s Syndrome
Hydroxyamphetaine(HA)
& Tyramine(TA)
Dilation→
no dilation
Postganglionic
Cocaine(C) Dilation→
No dilation
Pre-,postganglionic
Epinephrine(E) No dilation→
Dilation
Postganglionic
Ganglionectomy
Mouse(C57BL/6)
Male, 30g, 4 mice
Anesthesia
(Ketamine+Xylazine)
Fix it on the bench and preparation of instruments
Surgery
Toe & tail
Ganglionectomy
Shave Incision Find SCG Cut SCG (right side) Suture back the fats &
the skin
Ganglionectomy
Measurement of pupil size
Anesthetized Ketamine pentobarbital
Conscious Tube Cone
Ruler
Eye Drop Test
Solution (saline) 1 micro-liter, twice, on each eye Record every 5-10mins Experimental time: 70mins
M: mouse number M0: normal mouse (day): the day after surgery
Result- Hydroxyamphetamine & Tyramine
Conscious Ketamine Pentobarbital
HA (1%) M1(9),M2(8),
M0
M1(7),M2(6) M1(10)
HA (0.1%) M2(7)
TA (5%) M1(15),M2(14),M3(7),M0
TA (1%) M1(16),M2(15),M3(8)
TA (0.1%) M1(17),M2(16),M3(9)
Result- Cocaine
Conscious Pentobarbital
Cocaine M1(18),
M2(17),
M4(1,3),M0
M2(9)
Result- Epinephrine
Conscious
Epinephrine 0.1% M1(10)
M2(13)
M3(6,10,15)
M4(8)
M0
Discussion
Compare to rabbits: Cocaine─ 5~30mins Hydroxyamphetamine─ 30~45mins
Cocaine, hydroxyamphetamine, and tyramine work on rabbits but not mice.
Only using epinephrine at certain day after surgery(10th-15th days) can have significant result to confirm the success of surgery.
Furthermore
Increase the number of experiment Find a critical way to measure the pupil size of
mice Electrode stimulation The mechanism of drug effect
Life in San Diego
Life in San Diego
Balboa park
Life in San Diego
Mission bay & Balboa park
University of California,San Diego
Campus