xiap09-06-01

XX-linked inhibitor of apoptosis proteinXIAPcaspase pET151-XIAPNi-NTAOD600 0.630IPTG0.5ug/ml4hpHpH 5.4pH 4.9

XIAP

pET151-XIAPJMY109RossetaLB--IPTGTrisNaH2PO4SDS-PAGE30% 1.5mol/L Tris(pH8.8)10%SDS 10% TEMED 1mol/L Tris(pH6.8)

pET1511.CaCl2 2.IPTGOD1.Ni-NTA 2.

1.OD

OD0.6OD37IPTG5ug/ml4hOD0.6-1.0

2.

OD0.6,IPTGIPTG5ug/ml4h30

3. IPTG

30OD600 0.64hIPTG0.5ug/ml0.5ug/mlIPTGIPTG0.5ug/ml

4.

30OD600 0.6IPTG0.5ug/ml4-8h10h 430OD600 0.6IPTG0.5ug/ml4h,XIAP

Ni-NTAMMarker A B W 5.9pH4.5:pH

Ni-NTASDS-PAGEMMarker A B W 5.9pH4.5:pH

Ni-NTASDS-PAGEPHMMarker A B W 5.9pH4.5:pH 5.44.9pH

pHpH4.94.5HispH

1.2.1 2343.4.

1. OD600 0.630IPTG0.5ug/ml4h2. pH

Thanks

Caspases1

Caspases11caspase caspasescaspase 1caspase 11caspase 4caspase 2caspase 8caspase 9caspase 10caspase 3caspase 6caspase 7caspase 37PARPDFF-45DNA fragmentation factor-45DNADNAcaspase-6lamin Akeratin 18 caspase

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