Specific characteristics

of ASF in wild boar

Remains of domestic swine bristle

Examination of a wild boar carcass External signs

Wild boar carcasses

The examination showed pronounced

rigor of skeletal muscles

Different fragments of a wild boar carcass

Population welfare control

Diagnostic culling of wild boar and their

transportation to the place of examination

Loading of carcasses and their transportation to

the place of autopsy

Autopsy of animals

Preparation to carcass autopsy

The autopsy usually begins with chest

and abdominal cavities dissection

The spleen of an ASF affected animal is

greatly enlarged and hyperemic (deep red

to black in color)…

… whereas in case of CSF tubercle-shaped

hemorrhages can be seen on the surface of the

spleen and hemorrhagic infarcts are easily

identifiable on its edge (see arrows), which is

absolutely not the case with ASF.

The kidneys of an ASF affected animal are

filled with blood and show multiple

hemorrhages (petechia) in medulla and renal

cortex…

…where as in case of CSF the kidneys are

white-blooded and riddled with confluent

hemorrhages. (see arrows)

Hemorrhagic lymphadenitis of portal nodes in

an ASF affected animal

In an ASF affected animal mesenteric lymph

nodes are enlarged and resemble dark-red

ropes. This sign is characteristic only of ASF.

Unlike ASF in case of CSF lymph nodes

are swollen, dark-red in color and show

marble pattern when cut open

Unlike ASF in case of CSF mucous

membrane of larynx especially in

epiglottis is seen to have petechial

hemorrhages and is covered with plaque. Photos by Plum Island Animal Disease Center, USA

Selection of biological samples for laboratory tests

and analyses.

Accompanying documents

If a wild boar carcass has partially decayed or been consumed by

carnivores the remaining parts of organs and tissues (i.e. long

bone, internal organs, muscle tissue, hide) can be used as

pathological samples.

Intra-vitam sample collection Post-mortem sample collection

Blood samples with anticoagulant.

0.5-1 cm3 of secretion and excretion

samples

5-10 insect vectors

Samples of internal organs (lymph

nodes, spleen, tonsils, kidney, lungs,

liver) weighing 10 to 15 gram.

Marrow samples (from the long bone)

0.5 -1 cm3 of blood samples (from the

heart)

5-10 insect vectors

Biological samples should be taken as quickly as possible and placed in aseptic

specimen containers. Containers should then be hermetically sealed, marked,

frozen and placed in a medical transfer tote which in its turn should be

transported to a diagnostic lab with accompanying documents attached to it. All

safety precautions must be observed during the procedure

Measures to be taken if ASF is

suspected or during its eradication

in wild boar population

Earth excavation to the depth of 2-5m

Preparation of the flooring

Preparation for destruction

Wild boar carcasses being burned down at the place of discovery

Soil disinfection