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Supplemental Figure 1 – Mountain plots of serum E2 concentrations in LC-MS (MS) and five commercial E2 immunoassays (A-E) in 101 sera from healthy, asymptomatic men over the age of 40 years. The x axis is the bias from the reference method (LC-MS) and the y axis represents the cumulative distribution of values from the reference panel.

Bias vs MS (pmol/L)

-100 -50 0 50 100 150 200 250 300

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clinchem.2013.213363 1

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