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Title On the Gametophytes of Some Japanese Species Laminariales IV

Author(s) Kanda, Tiyoiti

Citation 北海道帝國大學理學部海藻研究所歐文報告, 2(2), 293-308

Issue Date 1941-02

Doc URL http://hdl.handle.net/2115/48071

Type bulletin (article)

File Information 2(2)_293-308.pdf

Hokkaido University Collection of Scholarly and Academic Papers : HUSCAP

On the Gametophytes of Some Japanese Species of Laminariales IV.

B}-

TIyolTI KANDA

'XYith I'late IJIX.

.In the ])resena' papei' t'hei'e a]'e des(tribed tl)e results otf eujtutre experi-

mei)1's ivith t'1)e g'ametoph)Tt'es tmd i'lie yoinig spot'oph>'tes of two speeies o£

Japanese Ijamiiitu'iales, viz.. L･amiitai'ia a"gi{s'ta't(t I<-ii,ijL"･i. an(l .,l.g(o'tcm,

ct'ibi'osunt ]'}oR>r. Citlt'ures ivere mad.e at the. sea-side ]abo]ratoi`y o'{ii t}}e t'llnstitnt'e of Als.e]og"ictal Resetureli at iX'Iiiyo]'a]i, b.x.' the stune ]nelho(ls as

l/liose dese]'i}/)e(l in (/.he pireiviious iyork. rt"he mate.vials usecl .in the present

"'oi･k "'ei'e eb('ained at tlie eoast ot'. iX･/[m'eran. Tl}e <tu'ltu]'e sohition 'tva,s

]nade eti i]at/tiral sea-"'atc}i', 'fiitei'ed tiviee throiEg,l) a layer o{'. abso]'bent

eott'on. Small (luanti.t.ies o'li sodiinn nit]'at/e aHd i}al/yi.um l.)hos],)hate "tvere

adcle(.l at the. /i'atio o£ the 't'ollo"'itigr table ivhieh has been ire(tomme]ided b>'

SC,Ill-lI･1IBl£R (1.9:-lL)).

NaN():s,.......{).lg'r distil]edxyat'ert.)Oct(r

NaL,IIPO.L.....O.02.g'i' sp.a-iyater l,OOOecAIat'ei'.ials beai'h]g "'e]hnatiu'ed s{>]ri xvei'e e(}llec(/e(/l at' the sea-shove, b)'ous,'ht

to the ]aboratoi:v, xvashed eturefuH>' in sterMzed sea-watei'. 1"hen the b]a(les

wei'e put int{} a g,Iass vessel eo]itainingr a,bout eig.ht/ likres o/£ txviee fi]tere(l

sea-ivate/i,' and the vessel "'as ilkm}inated "v]lth an ele(rtric ]tt.mp, that

exerte(l a g'ood efTeet' 'ffor the libeiration. of zoospo,res. [Vhe zoospo]res ivere

set･t'led on tlie. slide-g,lasses, an(L euKtu'e(1 in tk (tultu're vessel. eontaining

about' 2;V)(}e(t o'fknedium. rl"he eriltiu'e vessels weire plaeed i,n a )room xvhet'e

tlte:v iye]'e ]]ever ex})osed te (l.ireet sunlig,'1it. rl"he (i.u]tmre,s iveire sub;ieete{'l

to the fiiietiititioii o'{'. d'lie temp{ii'ti.tm'e oll the 'i'oo]ii.

]Se'1'oi'e g.oing,' l'u]'t'hei' the writei' iyjshes ('o eNpyess his k)est thanks l/o

IP]'o'fessor Y. ¥.x]Ng',ND.y ltor ltis l<iii(l g'iti(lamte tn}(l. crit'i{eisms; in. the (rou'rse o£

t'']te pi'ese]i(' st'll(ILv.

XVL Laminaria angustata I(.)i･;Li.]M.

IVI}e p]'esent speeie$ is o'ne o'f' t'he most' eomtnon alfL.ae oi} the eeast of

)Iurora]i. It, gi･o"'s oii roeks in the Io"Ter littoral aiid sub]ittoral belt,

assoeiated with A.larict erctss･ifolict KJELLM. The zoosporangia appeai" in

autumn and winter oll the eoast of Muroran. After the zoospores have

been ]iberated, the g,'reater part of the blade is gradually worn away, and

onl:r t･1]e stipe bea]iiig)' a eer'taill portion of the blade remains attaehed to

the substratum. The b].ade praetieally eeases to develop further during

autumn and ear]y w･illter; then in the earls months of the next >.rear, the

seeond year blade begins its sudden growth at the transition regio]i, pushing

up the first sear blade. The seeond year blade inereases in ]ength and

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' ' ' A r,.., ' IBiig. 1. Laoniota?'ia angustata KJEIjLM.

at Muroran in May.

gradual]s eorda,te; eollected in August.

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On t,lie Gametopliytes of Some Japa]iese Speeies of ]ljaminariales IV 295

thiekness to attai]i its £ull s.rowth i}) Aug?ust and Septembey and then

beeomes sori£erous. On the coast o£ MlureraB, the embryena} fyoAds appear

£vom January to Mareh and attain thely adult stage in August. s The zoosporangia are pyodrieed at first on one side o£ the blade, tl}e

£afieia on t･hat slde being projeeted, 2i} two parallel rows, Ieaving the £aseia

and both margins sterile. Later, h.owever, these two payallel rows join

into one in seveyal plaees, espeeially in the lower poytioll o£ the blade.Fully matuyed sori are dark olive in eoloar.

Caltuyes have been made t･hree times, hitherto, and.the same results

were obtained in eveyy ease.

Zoospores alld their Germination

[i]he zoospores aye pea"shaped when they aMe aetive, pointed at one

eiLd aiiLd rovindeCt at the other, measuring aborLt 8-9.2,u in length, 4-4.5sL

in byeadth; t･hey h.ave two latera}Iy plaeed eilia, ene pointin.ew £orwayd, as

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IEi'ig. 2. Laon･i･n(ti"･ia an.y･ustata KJELLA[. Zoospores a.na tl]elr germinatien.

×1250. A. Zoospores in motile sta･te. B. Embr>rospore. C-D. Germinationo£ tlie embryospores. E-G･. Deyelopment of the germination tube; the eontents

of the embryospere migrate inte t,his tube; £rom 2 day eulture. K-K. Sporel-h}gs Sroni 4 day eult.ure, showing the first transverse wall. I[j-O. Early stages

of the male gametephytes, from 7-9 day eulture. P-T. Ear]y stages ef tlie

fe}nale gametophytes, eonsistiiig of only one ee]l; £rom 7-9 da}r e"lture.

296 g]. KANDAlong as 18-20@, and the other pointing baekwords, as long as about 6-8st.

The zoospbres have one eui'ved ehromatophore whieh oeeupies the posterior

part, one nueleus and some granular substanees, but always laek an eye-

spot (Fig. 2, A). The zoospores swim aetively at first, but sooner or later

beeome motionless. They lose their ellia be£ore loRg, beeome spheriea} inshape, invested with a membrane and Easten themselves t･o the slide-glass

(Fig. 2,B). A gemnination tube grows out of the embryospore (Fig. 2,

C-D). The germination tube inereases its lekgth more and meye, as the

eulture progyesses, and within 2 days it yeaehes about 20pa in Iength (?ig.

2,E). The distal end lllereases in diametei', and a wall is £ormed so as

to separate the distal end £rom t･he tube. When t･he p}ants are about 7-9

days old, £wo types of gametophytes may be distinguished. [I]hose belongingto one t>rpe eonsist of q number of cel}s as figured iR Fi.rv. 2,L-O. They

are ma}e gametophytes. The others eonsist of only one cell at this stas,e,

the diamet,er of the eell, however, being mueh Iayger than that of the

£ormerJ t,hey. are Semaie gametophytes (lpsigi. 2, P-T).

Male Garnetophytes and Male Gametes

The male gametophytes of the present speeies eonsist of a fairly }arge

numbey of eells, the diameter of eaeh eell measux'lllg alNNTays less than 9,u.

When the male gametophytes approaeh t･o mat･urity the antheridia} eells

are produeed at the tips o£ t･he brallehlets. The ehromatophores o£ these

eells beeome pale in eolor. The eonteRts o£ the ant･heyidium is entirely

used £or the foymation of a single antherozoid. At maturity the wall of

the alttheridium bursts open at the apex, £oyming a beak through whieh "a matured antherozoid swims out (Fig. 3). The male gamete is ovate oy

sometimes rather round in shape, measuring about 6,tt ln IeRgth, and 2.5-

3.5pa !n breadth. Two cElia of almost equal length measuring about 20,u

arise fyom the lateral side of the body. It has generally olie or two pale

eoloyed ehromatophores, but a}ways laeks an eye-spot (Fig. 3,ixi). The

]iberat!on of the male gamete £rorn the opening o£ the aBtheyidium was

observed fyom time to time, but the actual proeess of £ertilization with

the egg eell has not been observed. The durat2on o£ life o£ the malegametophytes is generally short, and in the 60 day eultuye the g'reater part

of them have disappeared.

Female Gametopbytes and Eggs

The shape and size of the £emale gametophytes of this plaBt show

some resemblai,iee to those o£ Laminaria Yeozdoaoza MiyABE (?ig. 4). Some

On the Ga･metopl)ytes o£ Some Japanese Speeies o£ Laminariales IV 297

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1[i'ig. 3. Laminar･ia angustata KJELL)i. Va･rious forms of the maie gamet,o-

phytes and male gametes, froin 15-2e aay eulture. x980. ni. Male gametes himotile sta.te, bearhig two ailia of almest equal leiigt.li, ],neasurea up to 18pt ixi

length.

conslst oE only one eell (I]"lg'. 4, A-I), otheys eoRsist o£ txNro or more eells

(Fig. 4, JLN). In some eases, a unieellular £emale gametophyte showlngeuyious appearanee was observect (F2g. 4,F--I). Wheii they eome tomaturity the whole vegetative eell ehanges into an oogoniam (Fig. 4, O-G).

At matr{rity the chyomat･ephores in the oogonium beeome ero"Tded togethey

298 T･. KANDA

xxTit･h tl}elr long axes pax'allel to that of the oogonium (IIiig. 4, D-G).

ebntent･s o£ the oogonium is then pressed out through the opening atapex, where lt xemains attaehed (Ii'ig. 5, A).

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La7}}iotao'ia aoiyztstata I<Ji£L-M. Various £orms of the fema.le

ga,inetophytes, from l5-20 day eultzire. ×867. A-II. One ee]led female gameto-phytes. D-G. One celled female gametophytes with mature oogonia; the eell

is deeidedly elongatea and the eliTomatophores in the oogonium eell are erowdea

toget,her at the apex. J-Adi. [Vwo eeliea female ganietophytes. N. [Vlaree eelled

female gametephytes.

Development of youRg Sporephytes

Wheii the egg is £eytilized the cell divls!on takes plaee (Fig. 5,B).

[Vhe ne"vxT spor'ophytes grow rapidly, and in the 30-50 day eultuye many o£

them develop oiie or more rhizoids (Fig. 5, C-D). Furthey deve}opment

is entirely similay to that o£ other Laminayiaeeous plants, and theye a.re nospeeial remarks to be offered.

i･tf,'stg.

S3'

On the Gan}etoph}'tes

?EOtli1rs

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J'apnneseSr)ec･ie$oEIJ:uuim"tria]eslV 299

J' ./:/:..1.1-".'g.

II(,]td]v.LA[. A. An ogg :iust discl:n'ge(t out- of

S.mn]l :tmoi}nt of vhismii, :tn(! cr]iro]ii:tto-

×6L'L'. IB. IIL'ftrll}r st}tge of the spoi'ophyte,

spQ]'opl}Lyte (Eeyeloping :t i'hizoi(l. fi{i t'he

II). I:'ni'thei' deye]ovnient o£ tlie $poi'oph:'te,

cribrosum l3oi{,y

"',idel,x, distrib'utted in this (rotm,t/ir,vi

' to as /Ear sout/'h. as Omt}, .EILomoi'i

oi'. IIokl<aid(')', an(I o,n, tlie {l'apan Sea,

to as £a]' sout}'i as the no.i,'th-eastei."n coast

sublitt/o/ral. belt, assoeiated with Pltylo-

l'l'ie coasl/ o'f', A･krro/irtm its g,]rowing zo.ne is

at a depth o£ sevei'al l'.atl](nns "r'he'ire t'he

.' ])ab.ito£t'hi.spla,ntis'veyye'harae- Laininarja(teous plants in this d'is,l/]riek

from late iii xv.ipte'r to eEuily in spying.

,a ",,

IMg. ;5. .La･mina)'ia a･nyit,ytttta

the oog'oi]ium, from iL)O clnLy eulhu'o.

pho];es :n'e le£t･ i.n the oogonimn eell.

ti]'om 20 da.v eu]tu]'e. ×6L)Lt. (t. '>rotmg

base, tfiro]n 50 du:' eu]ture. × !L);7iO.

f]'om L' moi]t'1i cult'ure. × 160.

XVIIII. Agarum

Jly(.oii"it･ ci'iL}i'ostfijt Boi{y /is rat/he±'

li; i$ /liound gro"'i]}gr !'}.'om the I)'Luri.]es

I.'rell'ectttre, alo.ngr t]ae I'a(ri:fie (roast

si(le it. ,is foiin(t fvo]n, Sas,halien

o'ii Ko]rea. It･ s,]]roxvs on ]'oeks in t}]e

spa(7i:it, a l<i])a ()/ff sea g,rass. On

limit'e(l onlx' iR the stiblittora] be]t/ -.ivtite].' js i,'at}ieir (li.iiet. Ai]. eeo]oo'ieal

tei.'isti(r, when eompa'i.'ed "'ith oti}ei.'

1]hese plants become so]ilierous

3oe {I], KAND.k

as･;･

tl

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IEig. 6. Aga)'tt･m eJ'ibi'ostt･m BoRy. Hnbit of [tn old pl:mt beftri]ig zoosporangia;

eo]leeted at )･Iuroran in )'11:'iv. tt

[I]he zoosp()rai]gia are produced at. first in the hollow･s surirounding the

perforations, or in the depressions on both surfaees of t･he b].ade, alNva.v, s

Ieaving narrois' borders a]oiig the perforations and both margins sterile.

The sporophlrte of Agai'ion c･r`ibiiosum BoRy is perennial, but the length

.

OntheGametophytesofSomeJapai}eseSpeeiesofLaminarialesIV 301

of time that it may live has not been observed exaetly. A defuiite seasonal

vayiatio]k of the vegetative £rond ii}ay not be reeog'nized ilt this speeies.

In ear}y spring, fyoin lpsebruary to Maych, ho"Tever, the seeond yeay b}ade

begins its growth at the transition region bet"reen blade and stipe. Tke

zoospoi'angia are pyodueed on. t･his newl.y developed part, while the oi{l

'partremainssterile. ' . The material £ey tl'].e preseiit iiives£igat,ion was eollecte(i on the eottst

o£ Muroyan. Cultures were made twiee, in Februar.xr 1935 and in Ap]ril1939.

I.f the material is £ully matured, t}}e zoospores are libeyated wlthinan hour, but the }iberation of zoospores is veyy pooy whei} eompaxed wlth

DX..i･l,isFF.;.,o'I･i･ '.i,,.'･･･ ',,i.,･,

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Zoospoi'e andllesting Zoospore.

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li'ig. 7. Agar･um cribroszt･nz BoBy.

A. Zoospe]'e iii moti]e state. B.

Germinatioii of the zoospore, from 6 day eulture.

eu]tttre; the eontents o£ the oi'iginal eell niigrateI.-K. Sporelings from 24 day e,ult･ure, shGwing

Three ee]lea gametopl)ytes £rom 24 day cultm'e.day eultui-e .

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its gennination ×970. C. Embryospore. ID-F. Sporelings from 12 day into distal part of the tube.

the fixst cell division. L-N.

O-Q. Gametopliytes from 30

302 T. KANbA

'that of othey Ilaminariaeeous p].ants. The thalli were removed as quiekly

･as possible, because the zoospores are liable to deeay if they we℃e ]e£t

'standing' too long, on aeeount of the brown j'uiee oozed out of £he thal}i..As was. observed in Eisenia and Eekloitia the zoospores of Agaru･]n

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0(･y

a

E3ize,

W.ge %

o. %g

Fig. 8. Agarum eribros2tm Borey. Developmellt･ o£ the gametophytes fyom20-42 day eulture. A. Fiiamentous gametophyte with irregular braiiehlets.

×44e. B. Irregularly })ranehea gametophyte from 30 day eult･ure. x665. C.

Ditto, frem 42 da,y eulimre. ×440. D. Filamentous gametophyte fi'om 20 day

eulture. ×665. E-Y'. Dit･to, £rom 30 day eulture. ×665. G-H. Ditto, filioin 20

day eulture. × 665.

OntheGametophytesoESomeJ'apftneseSx}eeieso£IJaminarialesIV 3e3

ci'ibros2em IBeRy aye easily poisoned by this byown juiee, so that the

k/'eatment oRght to be eayrled out･ as quiekly as possible.

So fay as the "Tritey is aware, iio eultural. expeyimen.ts of the zoospores

o£ the ge]],us Ayao'･iem have been made hitherto.

N<iiiii

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ve'v' wf･ ･" ･{Zi ti,i5,.:i

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x･i" .if,-..

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Wig. 9. 2fl.garu?n cribrosu7it BoR¥. Variotts forms ef the male g'ameto-

phytes, with elttsters ef niature tuitheridia a]id empty bealgs at the t,ips o£ tliebranches, from 30-40 day eult･ure. in. A'Ittle gfamete in motile stat,e. x ttt44,.

304

ssb" "

l. ./l/.//kg$'/k･'sili,S

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1p i ".'."ib .mte ' oOt' e.a"k .o.Qi .. e:g'tne/ 1 't,O've,,,,sa'O" //keg{lf,i.." sse//lii.ilU"i.'i ,-, ,,,,,,,i.ss "ri

Fig. .1.0. Agarum cribrosum Bony. phytes. A-B. Four eelled stage of tlie eulture. xrt54. C-D. Well nourished

eells, from 4e day eulture. ×554. eoils/isting of niiie eells, from 34 day

gametophyte with branehes. ×554. G-H. frcm 65 day eulture. x334.

Zoospores and th

p}aeed ellia, one curved

substaltees, but always laek an

g-,rw

rcgei"'t/

･l'E'ee.

asuei

Various ･ female female E. Iryegularly brmxelied £emale gametophyte eulture. Well developed female .crametophytes

eir Germination

The zoospoyes aye peay-shaped, about 8-9sc in length, have two laterally

ehyomatophore, one nueieus and some graBulay eye-spot (Fig'. 7,A). They swim aetively

..･e. as, di .e.1 kt,

ffll!l ' ' s. '

foyms o£ gametopliytes,

gametophytes

×554. M

A eo

Ql fflk gi y

'vss..gede･,,l{.tY"'re

B E･ff･Sffi,lili,,,.,g,,,,L.l/･''"i}si:iiiii'

l¥klbdi'

-fa. ggmetaj

asglg

g(],:glttttf 'ge(s;

<ll$l,

ee/, Setiy-

the female gameto-

from 28 da}reonsisting of many

Filamentous female

On tlie Gametopl)ytes ef Some [l'ap:/tnese Sp. eaies o£ I]amina'riales IV 305

at fiyst, but soonei' or iater they beeome motion}ess. Whey lose their eilia

be£ox'e loxig, beeome spherieal i]i shape, invested wi£h a, membrane and

£asten themselves to the s}ide-glass (l]"ig. 7,IB-C). Within 6 days aftey

they have se£tled, a germinatioll tttbe gyows oat from the spore (Fis.. 7,

D-F). The dlstal end of the tube swells np, aBd a £ransverse xvall is

£ormed so as to separate it from the tube (Flg. 7, G-II). As the eu}tureprog]resses, t}]is enlarged distal end gradua}1>r liiereases lts }engthJ wlt}]in

it

o

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B

tw･ M "gbytrb?utIlk, s.,,] 'in.-u ･% ,

sctt'g"ag13,sgilliSr E3SJ..,.f

eqt2}EIEIa-t".r.y',".e.,enh'bl

Agaraon･ cj'･ib7'osum Boiiy. Female

oogoi]ia and egg-eells, and the

va -e&uorJ$iiE?{lp

.eeox""tYE

ge

',e' ,.'

lpaptf:3SSI;

i g.'ti)".mpS

J. .".''.eocwr

,91ms;ysptw

puig. ll. g'ametopliytes, showing theformation of developmeiit o£ the }roung sporo-phytes. ×312. A. Well (lexrelepea female gametopl}Jrtes with oogonia anC{ egg-eells, Erom 4e day eulhire. B-C. iE'enia]e gnmetopl]ytes eonsisting of irregulaxly

brttnehea filamen,{,. D. Fem:/tle gfimei'ophste wvith >'oung sporopliyte.,

306 ll]. KANDA

/

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t. 9g."Az.ls.l.lfi lit,.

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,,es]e<3';'

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oo

.

"･xl'

IE"i,g. I2. Aga･ruon ci'ibros･u7n BoR,y. Il]iemale

sporophytes. ×320. A. An egg resting at the openmgshape of tlae female gametophyte is vei'y eha･raeteristie.

phytes and au egg cell deve]oped from single female

B'urthei' developmellt o£ young sporop}iytes, from 40 day eulture.

., %ee"`'#tiftititttlill.c'(o,¥o

p "

o oi t"g er .di.

trS/ I}I{;ji.d',t･;'i .v/;; e.reE.o:s.t.f'{k;ge6Ifl.iiglttg'"./i.t/[..ir'v

gametophytes and young

' o£theoogonittm. The B. Two young sporo- gamotophyte. (FD.

OntheGametophyteso£SomeJapaneseSpeeiesof:"aminarialesIXT 307

24 days, t,he plants are divided into £zzr･o or moye eells by transveyse wa].ls,

to give a filamentous appeara}iee, t･he ],eRgth o£ t･he single eel} measmrii]sr

up to 22,Lt, whi}e £he breadth o£ a eell does not exeeed 8,u (Fig. 7, I-N).[l]he geiiera} appearance o.f the gametophytes at t･his stag'e is ver>' e}'tayae-

t,eristie, and shows a styong yesemblanee to that o£ Clostaf'ia- costa･ta･ ([VuR,/N.)

SAUND.

After £his, the byeadth o£ eaeh eel} gradually in,eyeases, and in tke30 da>r eu}tuwe, the plants shoxxr loosely branehed filaments eonsistiug o/ff

seveural eells (Fig. 7, O-Q, IJ'ig'. 8). {l]he differentiation of the two types of

gametophytes may be reeogn.!zed at this st,age.

Male Gametophytes aRd Male Game£es ' As the aceompanying figuyes show, tl,ie shape aiid general appeaya]iee

of the ma}e gametopliytes of 'this plant are ve]ry eharae-

teristie, and exeeedingl>r

diffevenl] £i'orn those of La?n･i-

na･ria, E21senilct, Ecklonila, e£e.

They £orm a}i iryegularlybranched filaineiit eon.sisth}o' oof a lars.e iimnber o£ eells(Flg`. 9). 'When the malegametop}'iytes appyoach tomaturity small eells ax'e eut

of at t}'ie apex of the braiiehes,

£orm2ng a eluster of antheyi-

dia (Ii'ig. 9). At maturity

the antherozoids are pressed

out £rom £he opening at thetips of the antheridia. They

aye pear-shapea, measurlngabout <F5,ce in length, have

two lateral}y plaeect e!lia of

almost equal lellgth, blit

neither ehromatophore n.oyeye-spot is observed.

, '

Female

The shape and size o.£ t･he

BA

,x

er ,

Fig.13. Agar･ztmeribrosumBoRy. Develop-

ment e£ the young sporopliyt･es. A-B. Yom}g,

sporophytes wit･h numerous i'h'izolds. ×].20. C.

Ditto, fi'om 40 da}r eulture. × 23e.

Gametophytes aMd Eggs

feiT}aie gametophytes as wel.1 as t.he diameter

3e8 C:. KANDAo£ eaeh eell o£ this plant is wide}y vax'iabie. Soixie eonsist of a ratbey

smali nmnber of cells (Fig. 10, F-[E[, Fig. 11). When the female gameto-

phyte ls between 40-50 days old, some cells o£ t}xe gametophyte metamor-

phose !nto an oogonium (Fi.cr. 11). The ehromatophores o£ the oogoniumee}I beeome small ii} size, and the eolltei}ts beeome more liquefied, exerting

strong inteynal pressure. At matuyity t}}e egg is pressed out tlirough the

opening', and remains attael}ed there (Flg. 11).

Deyelopment of young Sporo})hy£e

[[)he first eell Clivisioi} oE t･he £ertilizedt egg'-eell may be observe(! i.n the

40 days cRlture. (8'ig. Il,D). The rliizoids ma}<e their appearanee at the

base o£ t}}e sporophyte. A dise-shaped expa･nsion develops at t･he base o£

t.he blade as in o£her eases. Tkeye are i}o spee2a} remax'I<s to be offeyect on

the Clevelepmeiit o£ t,he yomig sperophytes (I]"ig. 13).

Discussion and Summaary

l. Most of the £emale gametophytes o£ La7ninar･ia ao'tg･zestata KJELLM'.

are ullieellular, aiid show some x'esembiance to those o£ .l)aminarict YencZoa7zce

MIyABE.

2. Theye are no speeia} eliaraeterlsties to be yemai'1{ed oii the shape

and habit o£ the male gametophytes o£ tZ)a7nina･ria angustata KJEilanM., when

eompareCtNsTiththoseoieotherlllJaminarlaeeousplant･s. '

3. In Laoniozaria aotgiestata KJELL]M[. the iength o£ time necessary £oi'

the matuyation o£ both types o£ gametophytes is rat･her short3 wkhin 7-9

days a£ter t･he liberation o£ zoospores, two types oS game£ophytes ayedist,inguished, a,nd wit･hin 24 days t･hey reaeli maturity.

4. So £ar as t}}e proeess o£ germination o£ zoospore, t･he shape ai}dgene]ral appearanee of tl]e gametophytes aye eoneeyned, there exists a

remarkab}e yesemblaiiee between Agaruin cr･ibrosuon IBoRy and Uosta7"･ia

costa-ta ([I]vRN.) SAvND., espeeially the shape o£ t,}}e male gametophytes,

havins,' Ioosely branehed fiIamentous strReture gives a s£rikii}g eharaeteristie.

5. In Agaruon cribrost"n BoRy, the diffeerentiation o£ tNNro types o£g`ametoph,ytes was ebserved ln plants of 28 day euiture.

6. Tl}e zoospores, as xxrell as the male gametes of both speeies stttdied

i],i the Present woyk always laek an eye-spot.

.

PLAT：E　LIX

l.-

L>.

3.

4.

5.

ll)LATE 59

xtstcu'uni cr'ibj'osu･nt Boliy.

II"oux yeung svorophytes an(l two oogoniti, devclc)ped fyom sins.le female

gmnetopli}rte.

IYemale gametopliytes with /filan}entous appeariui{;e, bearillg oogo3iia.

Yotuig spei'ophytes an(l inatiu'ea oogo]iia.

We]l developed femal,e gtunetop}]yte with mature oogoi}itt, anCl an egg,

just diseha]'ged out of the oogollium.

Well aeve'!oped male gttmetophyte wit,}i profusel}' l)rtu}e}ieCt filament･.

CI]lic habit an(l gellcra'i aspect of the male giunetophytes tu'e very

eharaeteristie,

.

［KANDA］PLATE　59

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