Giới thiệu Anabio

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MAKING LIQUID B. CLAUSII SPORETHE INTRODUCTION

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B. clausii produces useful enzymes amylase, protease and vitamin B.

B. clausii is polyantibiotic resistant to: ampicillin, cephalosporin, erythromycin, lincomycin, neomycin, chloramphenicol, tetracycline …

B. clausii is a safe bacterium (belongs to General Regarded As Safe, GRAS)

The characters of B. clausii

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Heat resistance of B. clausii spore. The spores were suspended in PBS buffer and incubated at various temperatures for 20 minutes before viable count. A half of the viable count at room temperature is remained at 90oC.

The time course of B. clausii spore stored at room temperature. More than half of the viable count is remained after 2 years.

Acid resistance of B. clausii spore. The spores were suspended in low pH buffers to mimic the pH of intestinal tract for 8 hours. The remained living spores were estimated by viable count. More than half of the viable count is remained at pH 2.

The Stability of B. clausii spore

Data were done by KLEPT

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High stability in hot drinks up to 60oC:

could be used together with hot milk,

juice…

Long shelves-life for 2 years at even up to

40oC

Could be used together with antibiotics

Convenient use for babies and children

Competitive price

The advantage of liquid B. clausii spore

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The certificate for service of R&D and QC granted by the Key Laboratory of Enzyme and Protein Technology (KLEPT), VNU University of Science

The GMP certificateThe FDA certificate

The CERTIFICATE

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THE PRODUCT WAS MADE BY UNIVERSITY SPIN OFF COMPANY ANABIO R&D JSC

CEO: Nguyen Hoa Anh, PhD in Life Sciences15 years studying and working in Tohoku University, Japan

QC Manager: Nguyen Thi Mai Phuong PhD in Biochemistry4 years postdoctoral research associate, University of Rochester, NY, USA

R&D Manager: Nguyen Thi Van Anh PhD in Life Sciences 5 years for studying in Japan

Financial Manager: Nguyen Thi Hoang Mai, MAAccounting and Finance, University of Leeds, UK

Product Manager: Pham Tien Dat. BSc. in Computer Science, 5 years for studying in Hanoi University of Science and Technology

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The Factory structure

OfficeDirectorManagementFinancial, Marketing, Sale Reception

R&D at KLEPT Lab scaleR&D at ANABIO Pilot scale

R&D

Spore Making: Spore Generation, Spore Purification BFS: Full automatic machine of Blowing, Filling, Sealing and Packaging, 

QC at ANABIOQC at KLEPT

QC

Production:

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The R&D was done in KLEPT

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Making B. clausii spore at ANABIO

B. clausii spore maker

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Filling, labeling and packing at ANABIO

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The quality control was done in KLEPT

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The quality control was also done in ANABIO

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(ANABIO, KLEPT)

Mixing

Sterilized water

Filling

Sampling for analysis*

Packing

Sampling for analysis*

Purified B. clausii spore*

QC(ANABIO, KLEPT, NIN)

QC (ANABIO, KLEPT)

How the quality was control

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Sequence of 16S Ribosomal RNA

The assays for quality control

Microscopy observation

Viable count on agar plates

Antibiotic resistant

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Available in > 2700 pharmacies in 40 provinces of Vietnam 110 clinics in Vietnam 16 hospitals: Bach Mai, L'Hôpital Français de

Hanoi, Nhi Dong 1 – Ho Chi Minh City, Can Tho city …

The distribution

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Export to Pakistan, Philippines, Colombia, Mexico…The Export

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L'Hôpital Français de HanoiGood responses from the doctors

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Some research papers contributed by ANABIO

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