Slide 1

(1632-1723)

, ,

( - )

,

(1810-1882) (1819-1892) (1821-1902) (1844-1912) (1843-1905) (1862-1915)

(Ernst Abbe,1840 - 1905)

(Stefan W. Hell, 1962 - )

,

Microscopy Far field , , Near field , (evanescent waves)Full field

, s = A*sin2pi/T * (t x / v), A , T , t , x , v . T u = 1/T , A = 1, t = 0, , : s = sin2pi*x*u

: , . , , , . ,

-: ; n ;

- NA: d = / 2*NA . , = 550 , 275 / NA.

( ) - (DIC)

, ,

Catharanthus roseus

123 HEK-293 ( 4 .)

. 562

, , (G.Kreth et al., 2000)

(Ernst Abbe,1840 - 1905)

(Stefan W. Hell, 1962 - )

,

, PSF,

Stimulation Emission Depletion - STED

, 75 , () STED (B) [S. W. Hell et al., 2005] STEDStimulation EmissionDepletion Microscopy1994 - 199975 GSDGround State Depletion1995 - 200610 SIMStructured Illumination Microscopy2002 - 200585 RESOLFTReversible Saturable Optically Linear Fluorescence Transitions2003 - 200550 PALMPhotoactivated Localization Microscopy2006 - 201020 - 50 STORMStochastic Optical Reconstruction Microscopy 2006 - 200820 30

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