2.2

—


—

(—204)30mm

M—1%Triton X—100M—0.2%R2503.0%0.2mol/L3.7%—PEMD—
PEMPEMPPEMD FITC--PBS(9:1pH 8.5-9.0)

1%Triton X—10025—30 min37
M—3
PBS
40×
+++(70%80%)PBS
3.7%—PEMD10 minPBS–2035 min
20µl2025 minPBS32—PBS

——
0.5Triton X-100/PEMP37152min
PEMP2


35mmPBS→1%Triton X—100/PBS→PBS5min
40 µLFITC-(0.3%Triton X—100/PBS1:41:8)5371 h
6

—3.7%—PEMD-20

()
M-?