指頭血による生体酸化包括的評価法と糖質摂取惹起酸化ストレスの

ビタミン C による抑制

塩入 麻由 1, 井上 葉子 1,2, 矢部 えん 1

長浦 亜希子 1, ○ 薗田 勝 1

1 共立女子大・家政・食物栄養2 愛国短大・家政

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2-IV-23

☆ Zhang D. & Gutterman D. Am J Physiol Heart Circ Physiol (2008) 292, 2023-2031

☆ Sies H. et al. J Nutr (2005) 135, 969-972 ☆ Ceriello A. Diabetes (2005) 54, 1-7 ☆ Dandona P. et al. Am J Clin Nutr (2004) 80, 51-57 ☆ Nishikawa T. et al. Nature (2000) 404, 787-790 ☆ Ghanim H. et al. Diabetes Care (2007) 30, 1406-1411 ☆ Newsholme P. et al. J. Physiol (2007) 583.1, 9-24

Oxidative Stress Induced by Glucose-FluxOxidative Stress Induced by Glucose-Flux

PKC

gp91 p22

Rac

p67 NOX

p

p

p47

GLUT

Glucose

Gluc 6-P

Pentose-P-pathway

NADPH

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ROS

GLUT

Glucose

Gluc 6-P

Pyruvate

ETCMn-SOD

Cu, Zn-SOD

Glutathione-POD

Glutathione-Red

Antioxidants

UCP

O2Mt.

Oxidative Stress Induced by Glucose-FluxOxidative Stress Induced by Glucose-Flux

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AGE

ROS

Polyol Pathway

NOS Uncoupling

Whole Blood

Photosensitization

Post-Illumination

Hemolysis Profile

Propagation Reaction

Initiation Reaction

Evaluation Method for The Redox BalanceEvaluation Method for The Redox Balance

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2 20 40 60

Propagation (min)

0

50

100

%-H

emol

ysis

hν

O1

2

O1

2

MS : age 59, male MS : age 22, female

The Redox Balance < Glucose 75g >The Redox Balance < Glucose 75g >

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%-H

emol

ysis

2 20 40 60

Propagation (min)

0

50

100

Fasted

120 min60 min 30 min

2 20 40 60

Propagation (min)

O1

2 O1

2

The Redox Balance < Ascorbate 600mg >The Redox Balance < Ascorbate 600mg >

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MS : age 59, male MS : age 22, female

Fasted30, 60, 120 min later

100

2 20 40 60

Propagation (min)

0

50

%-H

emol

ysis

2 20 40 60

Propagation (min)

O1

2 O1

2

The Redox Balance < Sucrose 20g > < Asc & Suc >The Redox Balance < Sucrose 20g > < Asc & Suc >

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MS : age 22, female

%-H

emol

ysis

2 20 40 60

Propagation (min)

0

50

100

Sucrose : 30min

Fasted

2 20 40 60

Propagation (min)

Sucrose : 30min

Fasted

Ascorbate : 30min

O1

2 O1

2

The Redox Balance < Boiled Rice 360g >The Redox Balance < Boiled Rice 360g >

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MK : age 21, female EA : age 21, female

O1

2 O1

2

Propagation (min)

2 20 40 60

0

50

100

%-H

emol

ysis

Boiled Rice : 60minFasted

Boiled Rice : 120min

Propagation (min)

2 20 40 60

The Redox Balance < Boiled Rice 360g & Asc >The Redox Balance < Boiled Rice 360g & Asc >

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MS : age 22, female

O1

2 O1

2

Propagation (min)

2 20 40 60

0

50

100

%-H

emol

ysis Boiled Rice : 60min

Fasted

Boiled Rice 120min

2 20 40 60

Propagation (min)

Ascorbate : 30min

Asc － Asc ＋

Arbitrary Units for The Redox BalanceArbitrary Units for The Redox Balance

2 20 40 60

Propagation (min)

0

50

100

%-H

emol

ysis

Regression Slope

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AE

0

3.0

Arb

itra

ry U

nits

1.5

MKAE TK SY

Boiled Rice : 60minFasted

Boiled Rice : 120min

MK SY YK YH

360g (134g)

Sucrose : 60minFasted

Sucrose : 120min

20g180g (67g)

Redox Balance Changes by Glucose-FluxRedox Balance Changes by Glucose-Flux

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ConclusionConclusion

Nutritional oxidative stress, or postprandial oxidative stress ensueNutritional oxidative stress, or postprandial oxidative stress ensue

s from an imbalance between the reactive oxygen species generates from an imbalance between the reactive oxygen species generate

d and the antioxidative defence system.d and the antioxidative defence system.

Endogenous antioxidative capacity influenced by glucose-flux can Endogenous antioxidative capacity influenced by glucose-flux can

be recovered immediately in young, but remains a low-level in eldebe recovered immediately in young, but remains a low-level in elde

r without adequate supplementation of antioxidative vitamins. r without adequate supplementation of antioxidative vitamins.

The method employing a photosensitization technique would estimThe method employing a photosensitization technique would estim

ate comprehensively the endogenous antioxidative capacity, or the ate comprehensively the endogenous antioxidative capacity, or the

redox balance.redox balance.

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