DNADNASNP-1

DNASNP

,,, (Comparative Genomics)GC%

cDNAsyntenycollinearity

tree of life

DNA

1

2Clustal X

3MEGAPHYLIPPAUPhttp://www.megasoftware.net/

4

DNA UV-120

DNA

0.15M0.15MDNA

DNA 1. 2. DNADNADNADNA

15% SDSSDSSDSCHCl3/IAA--DNACHCl3/IAA

10min95-10s1

DNADNA---r595nmml20400gDNA

8100595nmDNAg/ml.DNAUB10min, 595nmBDNA

220-320nm260nmA260/A280 A260/A280 1.8 DNA > 1.8 RNA < 1.8

3601000nm150400nm UV-120

UV-120UVHeatON10min0-100%T0% TABS 0-20% A

0.01N NaOH 260nm280nmA260/A280DNA

SNPSNP(single nucleotide polymophism) 1 % SNP SNP1000

SNPSNP SNP(coding SNP, cSNP)SNPSNP(regulatory SNP, rSNP)cSNPcSNP (synonymous cSNP)cSNP (non-synonymous cSNP)cSNPPRKCHcSNP(1425G/A)GA cSNP1(MDR1)3435 SNP(3435C/T)MDR1 mRNA mRNA SNP(GCKR)16 SNP(rs780094, T/C)CT2rSNPIFN-rSNP(-764G/C)C G IFN-g 2~3

SNP

NCBISNPFascinEzrinNGAL receptorSNPSNPSNPSNPSNPSNPSNP

P1