Sakamoto & Nagatani, 1996 : Yamaguchi etal., 1999Nagatani, 2000Kong & Nagatani, 2008Endo et al., 2005, 2007Kong et al., 2006

GFP

A01

3

COR15am DehydrinLipocalinSynaptotagminSynaptotagmin COR15a CDRE COR15amDehydrinLipocalin

LUCLUC EMSEMS LUC

A01

4

mizu-kussei : miz MIZ1, MIZ2 MIZ1MIZ1MIZMIZ2 GNOMGNOMADP ribosylation factor ARF GDP ARF-GDP ARF-

GTP guanine nucleotide exchange factors GEF PIN1miz2

miz2 PIN1 Brefeldin A BFA BFA GNOM BFAGNOM gnomB/EGNOMMIZ1MIZ1MIZ2 GNOMMIZ1

2ABCD

1

A01

5

A01

A02

12

1photphotphot N- LOVC- / phot1 phot2photphot1phot2 phot1

2CryCryCry1 Cry2 Cry-DASHCry N- DNA Cry-DASH DNA CryCry3phy phy phyB phy 4MIZ1

6

1FM1-43

2GFP200mMNaCl

A02

1

NaCl

ERPYK10ER

7

A02

1

2ped11 GFP 2

32 The Plant Organelles Database : http://podb.nibb.ac.jp/Organellome/phyAphyBcry1cry2 phot1pho2 DCMU var23

3 GFP ER -glucosidase PYK10

4 glucosinolaes

98

4

3DarkLight+DCMU Light

4Control mutant12

4

1LG

2PMtChV

A02 A03

1 cDNA DNA

1

PCR 1 mRNA1 cDNA

9

A03

mRNA 85 cDNA

1

2 mRNA

RNA cDNA RNA cDNA RNA cDNA185cDNA1

11

10

A03

1 1

1

3 Ca2+ Ca2+ Fluo-8Ca2+

1 1

2

DNA mRNA2 3

23

FTMS

1110