7. LAMPIRAN · 7. LAMPIRAN Lampiran 1. Perhitungan L-Glutamic acid Ulva sp Tabel nilai Asorbansi kadar L-glutamic acid Ulva sp. Enzim Bromelin (%) Ulangan Waktu Inkubasi (jam) 1 3

37

7. LAMPIRAN

Lampiran 1. Perhitungan L-Glutamic acid Ulva sp

Tabel nilai Asorbansi kadar L-glutamic acid Ulva sp.

Enzim Bromelin (%) Ulangan

Waktu Inkubasi (jam)

1 3 5

∆A ∆A ∆A

5

1 0,0291 0,0416 0,0693

2 0,0315 0,0463 0,0625

3 0,0288 0,0534 0,0528

10

1 0,0476 0,0362 0,0313

2 0,049 0,0386 0,0363

3 0,0438 0,0348 0,032

Perhitungan enzim bromelin 5% dalam waktu 1 jam dalam 3x ulangan

Ulangan 1

∆A L-glutamic acid = 0,0291

𝑐 (𝑔

𝐿) =

2,9 ×147,13

19900×1×1× 0,0291 (

𝑔

𝐿)

𝑐 (𝑔

𝐿) = 6,239. 10−4

𝐾𝑎𝑑𝑎𝑟 𝐴𝑠𝑎𝑚 𝐺𝑙𝑢𝑡𝑎𝑚𝑎𝑡 (𝑔

100𝑔) =

6,239.10−4

100 (𝑔

𝐿)

× 100


100𝑔) = 6,239. 10−4

Ulangan 2

∆ALglutamic acid = 0,0488

𝑐 (𝑔

𝐿) =

2,9 ×147,13

19900×1×1× 0,0315 [𝑔/𝐿]

𝑐 (𝑔

𝐿) = 6,754. 10−4


100𝑔) =

6,754.10−4

100 (𝑔

𝐿)

× 100


100𝑔) = 6,754. 10−4

38

Ulangan 3


𝑐 (𝑔

𝐿) =

2,9 ×147,13

19900×1×1× 0,0288[𝑔/𝐿]

𝑐 (𝑔

𝐿) = 6,175. 10−4


100𝑔) =

6,175.10−4

100 (𝑔

𝐿)

× 100


100𝑔) = 6,175. 10−4


Ulangan 1


𝑐 (𝑔

𝐿) =

2,9 ×147,13

19900×1×1× 0,0416(

𝑔

𝐿)

𝑐 (𝑔

𝐿) = 8,919. 10−4


100𝑔) =

8,919.10−4

100 (𝑔

𝐿)

× 100


100𝑔) = 8,919. 10−4

Ulangan 2


𝑐 (𝑔

𝐿) =

2,9 ×147,13

19900×1×1× 0,0463[𝑔/𝐿]

𝑐 (𝑔

𝐿) = 9,927. 10−4


100𝑔) =

9,927.10−4

100 (𝑔

𝐿)

× 100


100𝑔) = 9,927. 10−4

Ulangan 3


𝑐 (𝑔

𝐿) =

2,9 ×147,13

19900×1×1× 0,0534[𝑔/𝐿]

𝑐 (𝑔

𝐿) = 11,449. 10−4

39


100𝑔) =

11,449.10−4

100 (𝑔

𝐿)

× 100


100𝑔) = 11,449. 10−4


Ulangan 1


𝑐 (𝑔

𝐿) =

2,9 ×147,13

19900×1×1× 0,0693(

𝑔

𝐿)

𝑐 (𝑔

𝐿) = 14,859. 10−4


100𝑔) =

14,859.10−4

100 (𝑔

𝐿)

× 100


100𝑔) = 14,859. 10−4

Ulangan 2


𝑐 (𝑔

𝐿) =

2,9 ×147,13

19900×1×1× 0,0625[𝑔/𝐿]

𝑐 (𝑔

𝐿) = 13,401. 10−4


100𝑔) =

13,401.10−4

100 (𝑔

𝐿)

× 100


100𝑔) = 13,401. 10−4

Ulangan 3


𝑐 (𝑔

𝐿) =

2,9 ×147,13

19900×1×1× 0,0528[𝑔/𝐿]

𝑐 (𝑔

𝐿) = 11,321. 10−4


100𝑔) =

11,321.10−4

100 (𝑔

𝐿)

× 100


100𝑔) = 11,321. 10−4

40


Ulangan 1


𝑐 (𝑔

𝐿) =

2,9 ×147,13

19900×1×1× 0,0693(

𝑔

𝐿)

𝑐 (𝑔

𝐿) = 10,206. 10−4


100𝑔) =

10,206.10−4

100 (𝑔

𝐿)

× 100


100𝑔) = 10,206. 10−4

Ulangan 2


𝑐 (𝑔

𝐿) =

2,9 ×147,13

19900×1×1× 0,049[𝑔/𝐿]

𝑐 (𝑔

𝐿) = 10,506. 10−4


100𝑔) =

10,506.10−4

100 (𝑔

𝐿)

× 100


100𝑔) = 10,506. 10−4

Ulangan 3


𝑐 (𝑔

𝐿) =

2,9 ×147,13

19900×1×1× 0,0438[𝑔/𝐿]

𝑐 (𝑔

𝐿) = 9,391. 10−4


100𝑔) =

9,391.10−4

100 (𝑔

𝐿)

× 100


100𝑔) = 9,391. 10−4


Ulangan 1


𝑐 (𝑔

𝐿) =

2,9 ×147,13

19900×1×1× 0,0362(

𝑔

𝐿)

41

𝑐 (𝑔

𝐿) = 7,762. 10−4


100𝑔) =

7,762.10−4

100 (𝑔

𝐿)

× 100


100𝑔) = 7,762. 10−4

Ulangan 2


𝑐 (𝑔

𝐿) =

2,9 ×147,13

19900×1×1× 0,0386[𝑔/𝐿]

𝑐 (𝑔

𝐿) = 8,27625. 10−4


100𝑔) =

8,27625.10−4

100 (𝑔

𝐿)

× 100


100𝑔) = 8,27625. 10−4

Ulangan 3


𝑐 (𝑔

𝐿) =

2,9 ×147,13

19900×1×1× 0,0348[𝑔/𝐿]

𝑐 (𝑔

𝐿) = 7,461. 10−4


100𝑔) =

7,461.10−4

100 (𝑔

𝐿)

× 100


100𝑔) = 7,461. 10−4


Ulangan 1


𝑐 (𝑔

𝐿) =

2,9 ×147,13

19900×1×1× 0,0313(

𝑔

𝐿)

𝑐 (𝑔

𝐿) = 6,711. 10−4


100𝑔) =

6,711.10−4

100 (𝑔

𝐿)

× 100


100𝑔) = 6,711. 10−4

42

Ulangan 2


𝑐 (𝑔

𝐿) =

2,9 ×147,13

19900×1×1× 0,0363[𝑔/𝐿]

𝑐 (𝑔

𝐿) = 7,7831. 10−4


100𝑔) =

7,7831.10−4

100 (𝑔

𝐿)

× 100


100𝑔) = 7,7831. 10−4

Ulangan 3


𝑐 (𝑔

𝐿) =

2,9 ×147,13

19900×1×1× 0,032[𝑔/𝐿]

𝑐 (𝑔

𝐿) = 6,861. 10−4


100𝑔) =

6,861.10−4

100 (𝑔

𝐿)

× 100


100𝑔) = 6,861. 10−4

Lampiran 2. Analisis Data Kadar L-glutamic Acid g/ 100 g protein dibenerin mg/g

Uji Normalitas Waktu Inkubasi

Tests of Normality

,185 6 ,200* ,923 6 ,525

,257 6 ,200* ,819 6 ,087

,248 6 ,200* ,878 6 ,262

tiga_jam

satu_jam

lima_jam

Statis tic df Sig. Statis tic df Sig.

Kolmogorov-Smirnova

Shapiro-Wilk

This is a lower bound of the true s ignificance.*.

Lil liefors Significance Correctiona.

43

Uji Normalitas % Enzim Bromelin

Independent sample T-Test dengan 2 Perlakuan enzim Bromelin 5% dan 10%

Uji Homogenitas Data

Test of Homogeneity of Variances

Levene Statistic df1 df2 Sig.

lima_persen 1,800 2 6 ,244

sepuluh_persen ,451 2 6 ,657

Uji One Way Anova dengan Perlakuan Waktu Inkubasi 1, 3 dan 5 Jam

Tests of Normality

,170 9 ,200* ,930 9 ,482

,209 9 ,200* ,910 9 ,314

lima_persen

sepuluh_persen

Statistic df Sig. Statistic df Sig.

Kolmogorov-Smirnova

Shapiro-Wilk

This is a lower bound of the true significance.*.


Independent Samples Test

1,048 ,364 -9,451 4 ,001 -,00036 ,00004 -,00047 -,00026

-9,451 3,305 ,002 -,00036 ,00004 -,00048 -,00025

2,475 ,191 2,883 4 ,045 ,00022 ,00008 ,00001 ,00043

2,883 2,408 ,082 ,00022 ,00008 -,00006 ,00050

2,228 ,210 5,528 4 ,005 ,00061 ,00011 ,00030 ,00091

5,528 2,415 ,020 ,00061 ,00011 ,00020 ,00101

Equal variances

assumed

Equal variances

not assumed

Equal variances

assumed

Equal variances

not assumed

Equal variances

assumed

Equal variances

not assumed

satu_jam

tiga_jam

lima_jam

F Sig.

Levene's Test for

Equality of Variances

t df Sig. (2-tailed)

Mean

Difference

Std. Error

Difference Lower Upper

95% Confidence

Interval of the

Difference

t-test for Equality of Means

44

Uji Duncan Multiple Range Test Kandungan Asam Glutamat pada Ulva sp. dengan

Perlakuan Waktu inkubasi 1, 3 dan 5 Jam

Lampiran 3. Analisis Data Kadar L-glutamic Acid g/ 100 g Berat Kering

ANOVA

,000 2 ,000 20,962 ,002

,000 6 ,000

,000 8

,000 2 ,000 24,655 ,001

,000 6 ,000

,000 8

Between Groups

Within Groups

Total

Between Groups

Within Groups

Total

lima_persen

sepuluh_persen

Sum of

Squares df Mean Square F Sig.

lima_persen

Duncana

3 ,0006

3 ,0010

3 ,0013

1,000 1,000 1,000

waktu

1_jam

3_jam

5_jam

Sig.

N 1 2 3

Subset for alpha = .05

Means for groups in homogeneous subsets are displayed.

Uses Harmonic Mean Sample Size = 3,000.a.

sepuluh_persen

Duncana

3 ,0007

3 ,0008

3 ,0010

,139 1,000

waktu

5_jam

3_jam

1_jam

Sig.

N 1 2




45

Uji Normalitas Waktu Inkubasi

Uji Normalitas % Enzim Bromelin

Independent sample T-Test dengan 2 Perlakuan enzim Bromelin 5% dan 10%

Uji Homogenitas Data

Tests of Normality

,263 6 ,200* ,821 6 ,089

,179 6 ,200* ,924 6 ,534

,249 6 ,200* ,875 6 ,247

satu_jam

tiga_jam

lima_jam

Statis tic df Sig. Statis tic df Sig.

Kolmogorov-Smirnova

Shapiro-Wilk

This is a lower bound of the true s ignificance.*.


Tests of Normality

,174 9 ,200* ,930 9 ,481

,208 9 ,200* ,905 9 ,283

lima_persen

sepuluh_persen

Statistic df Sig. Statistic df Sig.

Kolmogorov-Smirnova

Shapiro-Wilk

This is a lower bound of the true significance.*.


Independent Samples Test

1,499 ,288 -9,589 4 ,001 -,02562 ,00267 -,03304 -,01820

-9,589 3,109 ,002 -,02562 ,00267 -,03396 -,01728

2,470 ,191 2,931 4 ,043 ,01593 ,00543 ,00084 ,03101

2,931 2,414 ,079 ,01593 ,00543 -,00400 ,03585

2,246 ,208 5,626 4 ,005 ,04270 ,00759 ,02163 ,06378

5,626 2,422 ,019 ,04270 ,00759 ,01493 ,07048

Equal variances

assumed

Equal variances

not assumed

Equal variances

assumed

Equal variances

not assumed

Equal variances

assumed

Equal variances

not assumed

satu_jam

tiga_jam

lima_jam

F Sig.

Levene's Test for

Equality of Variances

t df Sig. (2-tailed)

Mean

Difference

Std. Error

Difference Lower Upper

95% Confidence

Interval of the

Difference

t-test for Equality of Means

46

Uji One Way Anova dengan Perlakuan Waktu Inkubasi 1, 3 dan 5 Jam

Uji Duncan Multiple Range Test Kandungan Asam Glutamat pada Ulva sp. dengan

Perlakuan Waktu inkubasi 1, 3 dan 5 Jam

Lampiran 4. Perhitungan Kadar Protein Ulva sp.

Test of Homogeneity of Variances

1,889 2 6 ,231

,432 2 6 ,668

lima_persen

sepuluh_persen

Levene

Statistic df1 df2 Sig.

ANOVA

,003 2 ,002 21,384 ,002

,000 6 ,000

,004 8

,001 2 ,000 24,761 ,001

,000 6 ,000

,001 8

Between Groups

Within Groups

Total

Between Groups

Within Groups

Total

lima_persen

sepuluh_persen

Sum of

Squares df Mean Square F Sig.

lima_persen

Duncana

3 ,0449

3 ,0710

3 ,0927

1,000 1,000 1,000

waktu_inkubas i

1_jam

3_jam

5_jam

Sig.

N 1 2 3




sepuluh_persen

Duncana

3 ,0500

3 ,0551

3 ,0705

,149 1,000

waktu_inkubas i

5_jam

3_jam

1_jam

Sig.

N 1 2




47

Kurva Standard BSA

Tabel Nilai Absorbansi Kurva Standard BSA pada Metode Bradford

Konsentrasi

(ppm)

Aquabidest

(mL)

BSA (mL) Absorbansi Persamaan Regresi

105 2,375 2,265 0,0927

y=0,001x-0,017

115 2,125 2,875 0,1040

125 1,875 3,125 0,1121

135 1,625 3,375 0,1260

145 1,375 3,625 0,1340

Gambar Kurva Standard BSA pada Metode Bradford

Perhitungan

Diketahui :

Volume Pelarut : 25 ml

Berat Awal : 0,025 g

Faktor Pengenceran : 1

Rumus :

𝐾𝑎𝑑𝑎𝑟 𝑃𝑟𝑜𝑡𝑒𝑖𝑛 (𝑚𝑔

𝑔) =

𝑥 × 𝑚𝑙 𝑝𝑒𝑙𝑎𝑟𝑢𝑡 × 𝑓𝑎𝑘𝑡𝑜𝑟 𝑝𝑒𝑛𝑔𝑒𝑛𝑐𝑒𝑟𝑎𝑛

𝑏𝑒𝑟𝑎𝑡 𝑎𝑤𝑎𝑙

48

Ulangan 1

Absorbansi Ulangan 1 : 0,0202

y = 0,001x-0,017

0,0202= 0,001x-0,017

x = 37,2ppm

x = 37,2mg/L

x = 37,2mg/1000ml


𝑔) =

37,2𝑚𝑔1000𝑚𝑙

× 25 𝑚𝑙 × 1

0,25 𝑔


𝑔) =

37,2𝑚𝑔

𝑔

Ulangan 2


y = 0,001x-0,017

0,0184= 0,001x-0,017

x = 35,4ppm

x = 35,4mg/L

x = 35,4 mg/1000ml


𝑔) =

35,4𝑚𝑔1000𝑚𝑙

× 25 𝑚𝑙 × 1

0,25 𝑔


𝑔) =

35,4𝑚𝑔

𝑔

Ulangan 3

49


y = 0,001x-0,017

0,0293= 0,001x-0,017

x = 46,3ppm

x = 46,3mg/L

x = 46,3mg/1000ml


𝑔) =

46,3𝑚𝑔1000𝑚𝑙

× 25 𝑚𝑙 × 1

0,25 𝑔


𝑔) =

46,3𝑚𝑔

𝑔

Rata-rata = (37,2+ 35,4+ 46,3)/3

= 39,63 mg/g

Lampiran 5, Kadar L-glutamic Acid Ulva sp.

kadar asam glutamat g/ 100 g protein

Enzim (%) ulangan Waktu (jam)

1 3 5

1 0,0624 0,0891948 0,1486

5 2 0,0675 0,0992721 0,134

3 0,0618 0,1144952 0,1132

1 0,1021 0,0776166 0,0671

10 2 0,1051 0,0827625 0,0778

3 0,0939 0,0746149 0,0686

50

7. LAMPIRANPerhitungan enzim bromelin 5% dalam waktu 1 jam dalam 3x ulanganPerhitungan enzim bromelin 5% dalam waktu 5 jam dalam 3x ulanganPerhitungan enzim bromelin 10% dalam waktu 1 jam dalam 3x ulanganPerhitungan enzim bromelin 10% dalam waktu 3 jam dalam 3x ulanganPerhitungan enzim bromelin 10% dalam waktu 5 jam dalam 3x ulangan

Documents

7. LAMPIRAN · 7. LAMPIRAN Lampiran 1. Perhitungan L-Glutamic acid Ulva sp Tabel nilai Asorbansi kadar L-glutamic acid Ulva sp. Enzim Bromelin (%) Ulangan Waktu Inkubasi (jam) 1 3