*72120

' 3:

* : G- - , .G- , (). : ' . . (G) - , .

* - / S P. , .

- . .

- . . : . . - " .

* ? " , . .

* ? (GB), -, .

* - : - ES - [S] V0

:

ES.

ES ( ) :

k1>>k2 . . K2 : V = k2[ES]

[S] [E] .

. , k-2[E][P] . , V=V0. . 10%~ V0.

* -

* - : - : , . : ES : ES: ES : [ES]:[Et] = [E] + [ES] [E] = [Et] - [ES]

* (Vmax) ( ) :

-

- Km:

:

V0 [ES]:

* - : (V0) [S]>>[E] . . , V0 - Vmax . , , (V0) .

* :

Km >> [S] : V0 [S]

[S] >> Km : [S]

Km = [S] : Km .

*kcat: - . , . turnover number. ( / 1).kcat / Km: . .

* Lineweaver - Burk - -

:

Lineweaver-Burk Equation

* :

.

:

.

.

*

*

*

*

*

* noncompetitive - , :

* noncompetitive-1/Km1/Vmax

* 1: Vmax - Km ( ): -Vmax ~140 M/min. Km . Km ~70 M/min - Km=10-5 M.

* 2:

-3 ,

? : ( Et) Vmax Vmax k2[ET] = .Km " : Km = /k1 (k-1 + k2) . , X ( Km) , Y ( Vmax ).

* 3:

- 0.05mM - 0.2mM( 4), 2.5. , - 5mM - 20mM 14% . ?

. , Km . 5mM . .

* 4: (E487K) . , ( , ). " . " " : CH3CHO + NAD+ CH3COO- + NADH +H+ : 1. .. . 2. Vmax ( ). 3. Km ( 30M ~7000 M) 4. 1.5: 3

**BOX 6-2 FIGURE 1 Competitive inhibition.*BOX 6-2 FIGURE 2 Uncompetitive inhibition.*BOX 6-2 FIGURE 2 Uncompetitive inhibition.*BOX 6-2 FIGURE 3 Mixed inhibition.