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!"#$10005404 2014 12125505 !" #$%&'()*+,-./012*34)56789 !"# $%& ' ( )*% + , - . 261021!" #$%&'( 89 )*+, 261053!" # $#$)-*)-./0123 :;%& 456789:;<=>?67@ABCDEF:GH+IAJKLMNmesenchymalstemcells MSCs OP+MNQR:STUVW'( X9Y9Z3[QZGH+I\]MN^_CDEF` 3~4 a: MSCs bcMNBd^_efQRgh`;<=ij<=kl89Qmb 0.5 1.5 3mT ` IFCC no[pqr stuvalkalinephosphatase ALP wsxyz{|}~45:gG&[pq+{osteocalcin OCN g:QWesternblot Q MAPKP38 Q:tuRdsB;<= MSCs P +QR:ST)* ;<=^g ALP :wsxy OCN:QU:Pd2 1.5~ 2mT <=89 MSCs P+QR>?b;<=^tuR MAPKP38 :xy P38  ¡ SB253580 ¢W;<=:P+£¤)+ <=^ MSCs P+QRdVW¥ P38 Q:¦w§¨<=>;<=+IAJKLMNP+QR ¡ ?@A3BCR322.71 R329.21 R339.5 DEFGHIJKL©ª«¬,-30900290 31270993 ); ¯°±²³´µ¶·¸¹º»¼ NCET100922 ); #$½,¾¿À»¼20120182 MNOPÁÂÃEmail drzlguo@163.com QRSThttp //www.cnki.net/kcms/detail/51.1095.R.20140303.1010.002.html 20140303 Effectsofstaticmagneticfieldonosteogenesisofbonemarrowmesenchymalstem cells GuoZhiliang TengHaijun WangLiang ZhangDahai LiXin ChengMin DepartmentofOrthopaedics No.89Hos pitalofPLA Weifang ShandongProvince 261021 ExperimentalCenterofMedicaleResearch WeifangMedicalCollege Weifang ShandongProvince 261053 China Abstract Objective ToevaluatetheeffectsofthestaticmagneticfieldSMF onbonemarrow mesenchymalstemcells MSCs osteogenicdifferentiationandexplorethepossiblemechanism.Methods Densitygradientcentrifugationisolatedratbonemarrowmononuclearcellswerecultured invitro .MSCsofthe 3rdand4thpassageswerechosenastargetcells.Afterosteogenicinduction theMSCswereexposedtoSMFat 0.5 1.5 2or3mT.Theactivityofalkalinephosphatase ALP wasassayedwithIFCC.Thecalcium nodesofMSCswerestainedbyalizarinred.TheconcentrationsofosteocalcinOCN andtype collagen weremeasuredbyradioimmunoassay.WesternblottingwasusedtodetectthephosphorylationofMAPK P38. SpecificP38inhibitorSB253580wasaddedintotheculturemediaofMSCstoinvestigatetheroleofP38inthis process. Results SMF especiallyat1.5and2mT increasedtheactivityofALP thenumberofcalcium nodesandtheconcentrationsofOCNandtype collageninMSCs.SMFactivatedMAPKP38signalingmole cule.BlockingtheP38signalpathwaybySB253580partiallyreversedtheeffectsofSMFonosteogenicdifferen tiationofMSCs. Conclusion SMFpromotestheosteogenicdifferentiationofMSCsandtheP38MAPKpath wayisinvolvedinthisprocess. Keywords staticmagneticfield bonemarrowmesenchymalstemcells osteogenesis P38signaling pathway SupportedbytheNationalNaturalScienceFoundationofChina 30900290 31270993 ), theProgramforNewCenturyExcellentTalentsofMinistryofEducation NCET100922 andtheScienceandTechnologyDevelopmentPlanofWeifangCity 20120182 .Correspondingauthor GuoZhiliang Email drzlguo@ 163.com ( 36 Ä( 12 Å 2014 Æ Ç 30 È ( É ' ) G - - Ê J Third Mil Med Univ Vol.36 No.12 Jun.30 2014

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Page 1: aammt.tmmu.edu.cnaammt.tmmu.edu.cn/Upload/PaperUpLoad/04eb187f-2eb2-429c-b3a8-0fd03d1… · +iajklmn (mesenchymalstemcells, MSCs)¸Ì˝˛§ˇoqr—Ñ:mn,Òcdef, Ół、efÔ§Õ8:p+ÑÖ,×ØÙÚÛ、łÜÑ

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EffectsofstaticmagneticfieldonosteogenesisofbonemarrowmesenchymalstemcellsGuoZhiliang1,TengHaijun1,WangLiang1,ZhangDahai1,LiXin2,ChengMin2(1DepartmentofOrthopaedics,No.89HospitalofPLA,Weifang,ShandongProvince,261021;2ExperimentalCenterofMedicaleResearch,WeifangMedicalCollege,Weifang,ShandongProvince,261053,China)

  [Abstract] Objective Toevaluatetheeffectsofthestaticmagneticfield(SMF)onbonemarrowmesenchymalstemcells(MSCs)osteogenicdifferentiationandexplorethepossiblemechanism.Methods Densitygradientcentrifugationisolatedratbonemarrowmononuclearcellswereculturedinvitro.MSCsofthe3rdand4thpassageswerechosenastargetcells.Afterosteogenicinduction,theMSCswereexposedtoSMFat0,0.5,1.5,2or3mT.Theactivityofalkalinephosphatase(ALP)wasassayedwithIFCC.ThecalciumnodesofMSCswerestainedbyalizarinred.Theconcentrationsofosteocalcin(OCN)andtypeⅠ collagenweremeasuredbyradioimmunoassay.WesternblottingwasusedtodetectthephosphorylationofMAPKP38.SpecificP38inhibitorSB253580wasaddedintotheculturemediaofMSCstoinvestigatetheroleofP38inthisprocess.Results SMF,especiallyat1.5and2mT,increasedtheactivityofALP,thenumberofcalciumnodesandtheconcentrationsofOCNandtypeⅠ collageninMSCs.SMFactivatedMAPKP38signalingmolecule.BlockingtheP38signalpathwaybySB253580partiallyreversedtheeffectsofSMFonosteogenicdifferentiationofMSCs.Conclusion SMFpromotestheosteogenicdifferentiationofMSCsandtheP38MAPKpathwayisinvolvedinthisprocess.  [Keywords] staticmagneticfield;bonemarrowmesenchymalstemcells;osteogenesis;P38signaling

pathway

SupportedbytheNationalNaturalScienceFoundationofChina(30900290,31270993),theProgramforNewCenturyExcellentTalentsofMinistryofEducation(NCET100922)andtheScienceandTechnologyDevelopmentPlanofWeifangCity(20120182).Correspondingauthor:GuoZhiliang,Email:drzlguo@163.com

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36Ä(

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2014Æ

6Ç

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J Third Mil Med Univ          Vol.36,No.12

Jun.30 2014

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