H s truyn nhit

MN CNG NGH CH BIN SC SN

TI

NG DNG CA H ENZYME N NHNG BIN I TRONG NGUYN LIU SC SN TRNG CNG NGHIP THNH PH H CH MINHVIN CNG NGH SINH HC-THC PHMGVHD: Nguyn Th Thanh Bnh

DANH SCH NHMSTTTN1Dng c Long2Hunh Th Bch Phng3Trn Th Thu H4Nguyn Th Nhi5Lm Th Thy Trang6L Th Thanh TrH enzyme ni ti trong sn phm sc snEnzyme thy phnEnzyme oxy haProteinLipidOxydaseCathepsinTripsinEntrokinaza CollagenaseChymotripsinPepsinCalpain

-Pancreatic lipase-Pregastric lipase

-Lipoxydaza-Catalase-Gluco oxydase

ng dng ca enzym ni ti trong ch bin sc sn

- Lm mm tht tiu ha v hp thu tt cc cht dinh dng- Tng mi v v hng thm sn phm sau ch bin tng gi tr cm quan, gi tr dinh dng

Tht heo nng (95%)Lc b gn mXayp gia vQut nhuynTrn uVo vinGi bng l vngGi bng l chuiLn menMui, ng, bt ngtDa heo (5%)Luc chnRa qua nc lnhTch mLm schPhi khTi phiDu nTi xayp nh hnhSn phmXt si rot, tiTiu nguyn ht QUY TRNH SN XUT NEM CHUA

Cc bin i ha sinh trong ch bin nem chuaQu trnh t chn ca tht trong ch bin nem chua nh phn ng thy phn protein ca enzym ni ti:

Protein peptid + acid amin

Ngoi ra, cn c qu trnh thy phn protein do cc VSV c trong tht

Caththepsin A, B, C, DpH thp

Enzym cathepsin A,B,C,D

Qu trnh ln men lactic:Trong iu kin ym kh, vi khun lactic phn gii ng to acid lactic v cc sn phm ph to mi v c trng ca nem chua

- Ln men ng hnh: S chuyn ho ng n giai on to thnh acid pyruvic. Acid ny c kh 2 nguyn t H nh hot ng ca enzyme lacticodehydrogenase tr thnh acid lactic (chim ti 90%).

- Ln men d hnh: sn phm to thnh ngoi acid lactic cn c mt lng ln cc sn phm ph khc nh aldehyde, keton, ru ethylic, CO2, mt s cht thm nh diacetyl ester.

Cht lng nem khng n nhQu trnh ln men kh kim sot v ph thuc vo nhiu yu t:S lng v chng loi vi sinh vt.S lng,nng , hot lc ca enzym ni tiCch bao giNhit Thi gian NHC IM CA QU TRNH SN XUT NEM CHUA TRUYN THNG

Enzym b sung lipaseEnzyme b sungENZYME THY PHNProtease t thc vtNhm Tn Ngun gc Nhit pHng dngEnzyme thy phnProtase t thc vtPapainNha ca thn,l ,qu u Nha kh:khng bin tnh trong 3h/100 oC>100oC mt hot tnh4.5-8.5Bin tnh trong mi trng pH12Lm mm tht,chng kh cngBromalainChi da,v da v qu 60oC vn cn gi hot tnh6-8ficinNha thn cy sung30-80oCTi u :50-65oC4-9Protease t thc vt

Papain t u Bromalain t daFicin t sungProase t ng vtnhmtnNgunNhit pHng dngEnzyme thy phnProtase t thc vtPepsinD dyTi u:40-50oC70oC:mt hot tnh4-5:bn>5.5:khng hot ngLm mm tht,chng kh cngTng tinh cht cm quanTripsinTy tng30-40oC90oC pH 6-8: mt hot tnhpH ti u:8pH thch hp:7.8-9.5chymotripsinTy tng30-50oC8-9Protease t vi sinh vtNhmtnNgun gcNhit (oC)pHng dngEnzyme thy phnVi khunBacillus subtilisNi bo40-806-7Tng tnh cm quanLm men theo munLm c tht mm miTiu ha sn phm tt hnB.Licherniform306.5B.thermorpoteoliticus90-1204.5-6.5Nm mcAspergillus oryzaseNi bo40-503-4A.Saitoi30-503-4.5A.terricola30-504.7-7.9Xa khunStreptomyces grieusNi bo20-30pH ti u :7.5-8S.FradiaeS.trerimsus14Protease t vi sinh vt

Vi khunX khunNm mcEnzyme b sungNhmTn Ngun gcNhit (oC)

pHng dngEnzyme thy phnlipasePancreaseTuyn ty ca heo40-456.5-9.5Ti u:7.5-8.5Thy phn trigyceride t nhin,du,cht boRhizopus spVi sinh vt35-406-7.5Ti u:7Thy phn cht bo mch ngnCandida cylindraceaVi sinh vt40-455-7.5Thy phn cht boMucor favanicusVi sinh vt40-455.5-8Ti u:7Thy phn acid bo mch di v ngn

Nhm Tn NgunNhit (oC)pHng dngOxy ha khoxioreductaseGluco oxidaseSn xut t Aspergillus niger v Penicillium notatum80oC trong 2 pht b bin tnh2.7-8.5Oxy ha gluco thnh acid gluconiclipoxidaseBt u55-606.5-9Oxy ha acid bo Enzyme b sungng dng ca enzym b sung trong sn xut nem chua Mc ch:Rt ngn thi gian ln menTng tc qu trnh lm chnTng cht lng sn phm, p ng nhu cu th trngLm n nh sn phm, bo qun tt hnPhng php b sung enzym: Thng s dng enzym b sung t thc vt, nm mc v VSV T vi sinh vt:Men Nem Chua(Neo-Xel 2010):dng cy vi khunlactic ca cc loinem chua nh: Lactobacillus.spp.Thc y qu trnh ln men hiu quB sung vo giai on p gia v.Lng s dng: 20gram men nem chua dng cho 10kg tht.

T thc vt: ch phm enzym papain v bromelainB sung vo giai on qut nhuyn

papain

S dng kt hp 3 chng: Lactobacillus plantarum, Pediococcus cerevisiae v Micrococcus varians. T l phi trn cc chng ln lt l 103 : 106 : 103 (cfu/g)

S dng ch phm sinh hc Starter culture BIO-NC : cc chng ging vi sinh vt c cc c tnh sinh hc tt nh sinh tng hp bacteriocin c kh nng khng khun cao, probiotic, hot lc proteaza cao nng cao cht lng sn phm nem chua.

CM N C V CC BN LNG NGHE!CH3 CO COOH + 2H

CH3 CHOH COOH

Lacticodehydrogenase