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DAFTAR ISI

HALAMAN JUDUL….................................................................................... i

HALAMAN PENGESAHAN.......................................................................... ii

SURAT PERNYATAAN................................................................................. iii

PRAKATA....................................................................................................... iv

DAFTAR ISI.................................................................................................... v

DAFTAR SINGKATAN ................................................................................. x

DAFTAR TABEL ........................................................................................... xi

DAFTAR GAMBAR ...................................................................................... xii

DAFTAR LAMPIRAN.................................................................................... xiii

INTISARI......................................................................................................... xiv

BAB I PENDAHULUAN…....................................................................... 1

1.1. Latar Belakang.......................................................................... 1

1.2. Rumusan Masalah .................................................................... 2

1.3. Tujuan Penelitian...................................................................... 3

1.3.1 Tujuan Umum…........................................................... 3

1.3.2 Tujuan Khusus….......................................................... 3

1.4. Manfaat Penelitian…................................................................ 3

1.4.1 Manfaat Teoritis............................................................ 3

1.4.2 Manfaat Praktis ............................................................. 4

BAB II TINJAUAN PUSTAKA.................................................................. 5

2.1. Luka Bakar ............................................................................. 5

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2.1.1. Penyembuhan Luka ...................................................... 5

2.1.1.1. Penyembuhan Primer....................................... 6

2.1.1.2. Penyembuhan Sekunder .................................. 8

2.1.2. Fase Penyembuhan Luka.............................................. 9

2.1.2.1. Fase Inflamasi .................................................. 9

2.1.2.2. Fase Proliferasi ................................................ 10

2.1.2.3. Fase Remodelling ............................................ 11

2.1.3. Faktor yang Mempengaruhi Penyembuhan Luka.......... 12

2.1.3.1. Faktor Sistemik................................................ 12

2.1.3.2. Faktor Lokal..................................................... 12

2.2. Pembuluh Darah ..................................................................... 13

2.3. Pembentukan Pembuluh Darah Baru ....................................... 14

2.4. Pengaruh Binahong pada Penambahan Pembuluh Darah ........ 16

2.5. Daun Binahong (Anredera Cordifolia) ................................... 17

2.5.1. Sistematika Tanaman..................................................... 17

2.5.2. Nama Daerah ................................................................. 17

2.5.3. Morfologi Tanaman....................................................... 17

2.5.4. Kandungan Kimia Tanaman.......................................... 17

2.5.4.1. Flavonoid ......................................................... 18

2.5.4.2. Alkaloid ........................................................... 18

2.5.4.3. Saponin ............................................................ 18

2.5.4.4. Glikosida.......................................................... 19

2.5.5 Manfaat Tanaman........................................................... 19

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2.6. Madu ........................................................................................ 20

2.7. Kerangka Teori ........................................................................ 21

2.8. Kerangka Konsep .................................................................... 21

2.9. Hipotesis ................................................................................... 22

BAB III METODE PENELITIAN ................................................................ 23

3.1. Jenis dan Rancangan Penelitian ............................................. 23

3.2. Variabel dan Definisi Operasional ......................................... 23

3.2.1. Variabel Penelitian ..................................................... 23

3.2.1.1. Variabel Bebas............................................. 23

3.2.1.2. Variabel Tergantung .................................... 23

3.2.2. Definisi Operasional .................................................. 23

3.2.2.1. Salep Ekstrak Daun Binahong .................... 23

3.2.2.2. Madu............................................................ 24

3.2.2.3. Jumlah Pembuluh Darah.............................. 24

3.3. Populasi dan Sampel .............................................................. 24

3.3.1. Populasi Penelitian ..................................................... 24

3.3.2. Sampel Penelitian ....................................................... 24

3.3.2.1. Kriteria Inklusi............................................. 24

3.3.2.2. Drop Out ...................................................... 25

3.4. Instrumen dan Bahan Penelitian............................................. 25

3.4.1. Instrumen .................................................................... 25

3.4.2. Bahan Penelitian ......................................................... 25

3.5. Cara Penelitian ....................................................................... 26

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3.5.1 Pengambilan Daun Binahong..................................... 26

3.5.1.1. Pembuatan Ekstrak Daun Binahong ............ 26

3.5.2 Pengelompokan dan Perlakuan Mencit ...................... 28

3.5.3. Lama Perlakuan.......................................................... 29

3.5.4. Pembuatan Preparat Parafin ....................................... 29

3.5.4.1. Fiksasi .......................................................... 29

3.5.4.2. Dehidrasi...................................................... 29

3.5.4.3. Clearing ....................................................... 30

3.5.4.4. Infiltrasi Parafin .......................................... 30

3.5.4.5. Pembuatan Blok Parafin ............................. 30

3.5.4.6. Pengecatan .................................................... 31

3.6. Alur Penelitian ....................................................................... 32

3.7. Tempat dan Waktu Penelitian ................................................ 33

3.8. Analisis Hasil ......................................................................... 33

BAB IV HASIL DAN PEMBAHASAN ......................................................... 34

4.1 Hasil Penelitian ..................................................................... 34

4.2 Pembahasan ........................................................................... 36

BAB V KESIMPULAN DAN SARAN........................................................ 39

5.1. Kesimpulan ............................................................................ 39

5.2. Saran....................................................................................... 40

DAFTAR PUSTAKA ...................................................................................... 41

LAMPIRAN .................................................................................................... 45