Dapus 2008dru-8.pdf

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Lampiran 1. Analisis konsentrasi asam lemak (AOCS official method Ce 1-62 1990) Sebanyak ± 25 mg sampel ditimbang secara tepat, kemudian ditambahkan

standar internal (C17 : 0) sebanyak 0.5 mg. Ke dalam campuran ditambahkan 1.5

ml NaOH metanolik 0.5 N, kemudian tabung diisi dengan gas N2, ditutup rapat,

dikocok dan dipanaskan dalam penangas air suhu 100 0C selama 5 menit. Setelah

tabung didinginkan, ke dalam campuran ditambahkan 2 ml BF3-metanol (14% b/v),

tabung diisi dengan gas N2 ditutup dan tabung divorteks selama 30 detik. Ke dalam

tabung segera ditambahkan larutan NaCl jenuh sebanyak 5 ml, diisi dengan gas N2

ditutup dan dikocok. Lapisan heksana dipisahkan dan dipindahkan ke dalam vial.

Fase metanol/air diekstrak kembali dengan 1 ml heksana dan hasil ekstraksi

digabung serta dilakukan penyaringan dengan Na2SO4 anhidrid. Sampel methil

ester asam lemak dikonsentratkan dengan hembusan gas N2 hingga volume ± 1 ml.

Sampel siap diinjeksikanke alat kromatografi gas. Kondisi pemisahan asam lemak

adalah suhu injektor 250 0C, suhu detektor 260 0C, suhu kolom awal 140 0C yang

dipertahankan selama 6menit, peningkatan suhu kolom kemudian adalah 3 0C per

menit sehingga suhu akhir 230 0C dan dipertahankan selama 20 menit. Gas

pembawa digunakan helium dengan tekanan 1 kg/cm2, sedangkan tekanan gas

Hidrogen dan udara masing-masing adalah 0.5 kg/cm2. Identifikasi dilakukan

dengan menggunakan standar methil ester asam lemak dan kuantitasi dilakukan

dengan perbandingan standar internal.

91

Lampiran 2. Koefisien variasi bobot awal ayam broiler umur 1 hari (DOC) penelitian tahap I

Perlakuan Ulangan 1 2 3 4 5 6 1 47 44 41 40 46 45

R1 2 40 42 45 47 46 40 3 46 41 43 44 45 48 4 42 45 46 41 45 45 1 41 42 43 40 44 45

R2 2 41 42 39 47 40 46 3 46 39 40 49 45 48 4 39 44 42 44 45 46 1 42 40 41 39 47 48

R3 2 48 45 40 47 46 48 3 42 43 39 45 50 46 4 42 41 49 46 51 46 1 44 42 44 49 49 50

R4 2 41 50 43 39 44 48 3 50 42 44 42 45 44 4 47 44 43 42 44 39 1 41 42 44 47 48 48

R5 2 43 46 38 46 42 40 3 45 52 47 46 42 43 4 43 41 43 46 46 45 1 42 41 46 40 44 40

R6 2 51 43 42 47 46 45 3 46 43 39 48 42 52 4 45 40 43 51 50 46 1 43 45 43 41 45 45

R7 2 44 45 40 39 47 46 3 46 47 48 45 42 43 4 38 43 45 45 46 49 1 42 42 47 42 46 46

R8 2 46 42 42 42 46 46 3 40 46 44 41 45 44 4 50 41 40 47 47 40 1 45 42 43 43 49 44

R9 2 45 42 43 43 49 44 3 39 42 42 42 44 46 4 48 48 48 45 37 40 Jumlah total 9534

Rataan 44.13889 STDEV 3.089241 KV 6.99891 %

92

Lampiran 3. Analisis statistika pengaruh tingkat penggunaan minyak ikan dan suplementasi vitamin E dalam ransum terhadap pertambahan bobot badan ayam broiler

Ulangan R1 R2 R3 R4 R5 R6 R7 R8 R9 2118 2007 1947 1848 1805 1741 1998 1687 1990 1764 1947 1606 1708 1746 1773 1642 1800 1959 1806 1741 1923 1888 1810 1944 1899 1930 1685 1875 1880 1867 1857 1624 1745 2144 1879 1713 Rataan 1891 1894 1836 1825 1746 1801 1921 1824 1837 Total 7563 7576 7343 7301 6985 7202 7682 7296 7347 Faktor M. Ikan 0 3 6 Total 0 7562.70 7575.58 7342.83 22481.12 505400607Vit. E 100 7301.00 6985.23 7202.27 21488.50 461755632 200 7682.23 7296.23 7346.95 22325.42 498424229 Total 22545.93 21857.05 21892.05 66295.03 Total 2 508319110 477730635 479261853

Sumber db JK KT Fhit F0.05 Perlakuan 8 91100.83 11387.6034 0.6122597 2.31 M. Ikan 2 25093.0183 12546.5092 0.6745688 3.35 Vit. E 2 47498.8846 23749.4423 1.27689961 3.35 M X E 4 18508.92 4627.23113 0.2487852 2.73 Galat 27 502181.17 18599.3026 Total 35 593282.00

93

Lampiran 4. Analisis statistika pengaruh tingkat penggunaan minyak ikan dan suplementasi vitamin E dalam ransum terhadap konsumsi ransum ayam broiler

Ulangan R1 R2 R3 R4 R5 R6 R7 R8 R9 4176 4048 3564 4169 3959 3941 3818 3929 3836 3472 3606 3319 4062 3928 3720 3552 3783 3307 3724 4252 3181 4101 3776 3652 3428 3675 3336 3777 4234 3372 3814 3424 3748 3555 3663 3541 Rataan 3787 4035 3359 4036 3772 3765 3588 3763 3505 Total 15149 16141 13436 16146 15087 15061 14353 15050 14019 Faktor M. Ikan 0 3 6 Total 0 15148.90 16140.53 13435.92 44725.34 2000356436Vit. E 100 16145.89 15086.78 15060.67 46293.33 2143072711 200 14352.75 15050.00 14019.21 43421.96 1885466224 Total 45647.54 46277.31 42515.79 134440.63 Total 2 2083697807 2141589009 1807592305

Sumber db JK KT Fhit F0.05 Perlakuan 8 1610557.07 201319.6343 4.510892403 2.31 M. Ikan 2 676485.3264 338242.6632 7.578874583 3.35 Vit. E 2 344506.1693 172253.0846 3.85960929 3.35 M X E 4 589565.58 147391.3948 3.302542869 2.73 Galat 27 1205001.06 44629.66889 Total 35 2815558.13 Perlakuan

Konsumsi (g)

SSR 0.05

LSR 0.05

R4 4036 R2 4035 1 2.91 306.85R1 3787 249 248 3.05 322.17R5 3772 265 263 16 3.14 331.15R6 3765 271 270 22 7 3.21 338.54R8 3763 274 273 25 9 3 3.27 344.88R7 3588 448 447 199 184 177 174 3.30 348.57R9 3505 532 530 282 267 260 258 83 3.34 352.27R3 3359 677 676 428 413 406 404 229 146 3.36 354.38

Perbedaan antar perlakuan

94

Lampiran 5. Analisis statistika pengaruh tingkat penggunaan minyak ikan dan suplementasi vitamin E dalam ransum terhadap konversi ransum ayam broiler

Ulangan R1 R2 R3 R4 R5 R6 R7 R8 R9

1 1.97 2.02 1.83 2.26 2.19 2.26 1.91 2.33 1.93 2 1.97 1.85 2.07 2.38 2.25 2.10 2.16 2.10 1.69 3 2.06 2.44 1.65 2.17 2.09 1.88 1.80 1.90 1.98 4 2.01 2.25 1.81 2.05 2.11 2.15 1.66 1.95 2.07

Rataan 2.00 2.14 1.84 2.22 2.16 2.10 1.88 2.07 1.92 Total 8.02 8.56 7.36 8.86 8.64 8.39 7.54 8.28 7.66

Faktor M. Ikan 0 3 6 Total 0 8.02 8.56 7.36 23.94 572.991 Vit. E 100 8.86 8.64 8.39 25.89 670.0912 200 7.54 8.28 7.66 23.48 551.515 Total 24.41 25.48 23.41 73.31 Total 2 596.0845 649.4374 547.9721

Sumber db JK KT Fhit F0.05

Perlakuan 8 0.56 0.070151 2.455567 2.31 M 2 0.179501 0.089751 3.141612 3.35 E 2 0.271437 0.135718 4.750662 3.35 ME 4 0.11 0.027568 0.964998 2.73 Galat 27 0.77 0.028568 Total 35 1.33

Signifikansi

M. Ikan

FC

Perbedaan Antar Perlakuan

SSR 0.05

LSR 0.05

a 3% 2.12 ab 0% 2.03 0.09 2.905 0.141742 b 6% 1.95 0.17 0.08 3.05 0.148817

Signifikansi

Vit. E

FC


SSR 0.05

LSR 0.05

a 100 ppm 2.16 b 0 ppm 1.99 0.16 2.905 0.141742b 200 ppm 1.96 0.20 0.04 3.05 0.148817

95

Lampiran 6. Analisis statistika pengaruh tingkat penggunaan minyak ikan dan suplementasi vitamin E dalam ransum terhadap titer antibodi setelah vaksin ND pertama

R1 R2 R3 R4 R5 R6 R7 R8 R9 6 6 6 5 7 5 6 6 5 4 7 5 7 5 6 6 4 5 6 6 8 6 7 4 6 6 6 6 6 5 6 6 6 5 6 7 6 5 5 5 5 7 9 5 6 5 6 5 6 6 6 4 6 5 5 7 7 5 6 5 5 5 4 6 6 6 5 6 5 6 7 7 7 6 4 6 6 4 6 5 9 4 5 4 6 5 5 6 6 6 8 6 6 6 5 4 5 5 5 3 6 6 5 5 7 7 6 7

5.5 6 5.58 5.67 5.75 5.33 5.92 5.58 6 66 72 67 68 69 64 71 67 72

Faktor M. Ikan 0 3 6 Total 0 66 72 67 205 42025

Vit. E 100 68 69 64 201 40401 200 71 67 72 210 44100 Total 205 208 203 616 379456 Total 2 42025 43264 41209

Sumber db JK KT Fhit F0.05 Perlakuan 8 5.185185 0.648148 0.597826 2.056 M 2 0.351852 0.175926 0.162267 3.106 E 2 1.12963 0.564815 0.520963 3.106 ME 4 3.703704 0.925926 0.854037 2.486 Galat 99 107.33 1.084175 Total 107 112.52

96

Lampiran 7. Analisis statistika pengaruh tingkat penggunaan minyak ikan dan suplementasi vitamin E dalam ransum terhadap titer antibodi setelah vaksin ND kedua

R1 R2 R3 R4 R5 R6 R7 R8 R9 7 5 8 8 7 7 8 8 8 5 7 8 9 7 8 6 8 8 6 6 8 5 7 7 6 7 8 7 7 6 10 7 9 9 8 10 7 8 7 9 7 8 7 8 7 7 7 8 8 6 9 7 7 10 6 5 7 8 8 6 7 7 11 4 6 6 8 8 8 7 8 11 7 4 8 8 8 6 6 8 11 5 7 6 9 10 7 7 8 9 6 8 7 10 8 10 8 8 8 6 7 7 9 8 10 6 7 8

6.08 6.42 7.17 8.42 7.58 7.92 7.00 7.67 9.08 73 77 86 101 91 95 84 92 109

Faktor M. Ikan 0 3 6 Total 0 73 77 86 236 55696 Vit. E 100 101 91 95 287 82369 200 84 92 109 285 81225 Total 258 260 290 808 652864 Total 2 66564 67600 84100

Sumber db JK KT Fhit F0.05 Perlakuan 8 85.12963 10.6412 8.646888 2.056 M 2 17.85185 8.925926 7.253078 3.106 E 2 46.35185 23.17593 18.83242 3.106 ME 4 20.92593 5.231481 4.251026 2.486 Galat 99 121.83 1.23064 Total 107 206.96

97

Perlakuan

Perbedaan Rataan Antar Perlakuan

SSR 0.05

LSR 0.05

Signi fikansi

R9 9.08 a R4 8.42 0.67 2.80 0.90 ab R6 7.92 1.17 0.50 2.95 0.94 bc R8 7.67 1.42 0.75 0.25 3.05 0.98 bc R5 7.58 1.50 0.83 0.33 0.08 3.12 1.00 bc R3 7.17 1.92 1.25 0.75 0.50 0.42 3.18 1.02 cd R7 7.00 2.08 1.42 0.92 0.67 0.58 0.17 3.22 1.03 cde R2 6.42 2.67 2.00 1.50 1.25 1.17 0.75 0.58 3.26 1.04 de R1 6.08 3.00 2.33 1.83 1.58 1.50 1.08 0.92 0.33 3.29 1.05 e

98

Lampiran 8. Analisis statistika pengaruh tingkat penggunaan minyak ikan dan suplementasi vitamin E dalam ransum terhadap titer antibodi setelah vaksin IBD

R1 R2 R3 R4 R5 R6 R7 R8 R9 0 1 1 2 2 3 3 3 2 3 3 3 4 3 3 3 1 2 3 2 1 2 3 4 3 2 2 3 2 2 2 3 3 3 3 3 2 3 2 4 2 2 2 2 3 2 2 2 2 2 3 3 4 4 2 2 2 2 3 3 4 2 3 2 2 3 2 3 2 3 3 3 2 4 2 2 3 2

2.11 2.13 2.22 2.44 2.63 2.88 2.89 2.56 2.67 19 17 20 22 21 23 26 23 24

Faktor M. Ikan 0 3 6 Total 0 19 17 20 56 3136 Vit. E 100 22 21 23 66 4356 200 26 23 24 73 5329 Total 67 61 67 Total 2 4489 3721 4489

Sumber db JK KT Fhit F0.05 Perlakuan 8 6.097222 0.762152778 1.270169 2.088 M 2 0.253105 0.126552707 0.210907 3.138 E* 2 4.725755 2.362877493 3.937865 3.138 ME 4 1.118362 0.279590456 0.465953 2.518 Galat 69 41.40 0.600040258 Total 77 47.50

Vitamin

E Perbedaan Rataan Antar Perlakuan

SSR 0.05

LSR 0.05

Signifikansi

200 2.703704 a 100 2.648148 0.055556 2.82325 0.428897 ab

0 2.225694 0.478009 0.422453704 2.971 0.451342 b

99

Lampiran 9. Analisis statistika pengaruh tingkat penggunaan minyak ikan dan suplementasi vitamin E dalam ransum terhadap eosinofil

R1 R2 R3 R4 R5 R6 R7 R8 R9 2 3 1 0 0 0 1 3 2 0 1 1 3 3 1 0 2 1 4 2 1 3 1 1 0 1 4 8 5 0 1 0 0 2 3 1 0 3 0 0 5 0 0 0 0 2 2 2 0 0 1 0 3 0 5 0 0 0 0 0 0 3 0 2 2 3 0 0 1 0 0 0

2.88 2.25 1.00 0.88 1.13 0.50 0.38 1.88 1.00

R1 R2 R3 R4 R5 R6 R7 R8 R9 8.13 9.97 5.74 0.00 0.00 0.00 5.74 9.97 8.13 0.00 5.74 5.74 9.97 9.97 5.74 0.00 8.13 5.74

11.54 8.13 5.74 9.97 5.74 5.74 0.00 5.74 11.54 16.43 12.92 0.00 5.74 0.00 0.00 8.13 9.97 5.74

0.00 9.97 0.00 0.00 12.92 0.00 0.00 0.00 0.00 8.13 8.13 8.13 0.00 0.00 5.74 0.00 9.97 0.00

12.92 0.00 0.00 0.00 0.00 0.00 0.00 9.97 0.00 8.13 8.13 9.97 0.00 0.00 5.74 0.00 0.00 0.00 8.16 7.87 4.42 3.21 3.58 2.87 1.73 6.72 3.89

65.28 63.00 35.32 25.69 28.63 22.96 13.87 53.77 31.15 Faktor M. Ikan 0 3 6 Total 0 65.2781727 62.9988875 35.3218356 163.598896 26764.5987Vit. E 100 25.6876141 28.6343587 22.9566819 77.2786546 5971.99046 200 13.8692728 53.76616 31.1454023 98.7808352 9757.6534 Total 104.83506 145.399406 89.4239198 10990.3897 21140.9873 7996.63744


Perlakuan 8 339.883402 42.4854252 2.20884657 2.096 M. Ikan 2 69.6697849 34.8348925 1.81109009 3.146

Vit. E 2 168.262622 84.1313109 4.3740449 3.146 M X E 4 101.950995 25.4877487 1.32512564 2.526 Galat 63 1211.75541 19.2342129 Total 71 1551.63882

Signifikansi Vit. E Eisonofil Perbedaan Antar Perlakuan SSR 0.05 LSR 0.05

a 0 6.82 b 200 4.12 2.70 2.83 2.53 b 100 3.22 3.60 0.90 2.98 2.67

100

Lampiran 10. Analisis statistika pengaruh tingkat penggunaan minyak ikan dan suplementasi vitamin E dalam ransum terhadap heterofil

R1 R2 R3 R4 R5 R6 R7 R8 R9 9 25 18 19 16 15 27 7 1 21 18 16 17 10 15 21 12 5 51 14 23 14 46 4 19 28 14 36 56 17 10 58 14 17 25 21 13 8 23 12 7 18 29 10 10 18 41 12 52 10 20 16 36 28 13 10 14 18 15 17 14 2 13 29 54 43 13 42 5 38 30 22

23.75 28.25 20.75 19.38 25.50 13.50 22.63 18.75 14.25

R1 R2 R3 R4 R5 R6 R7 R8 R9 17.46 30.00 25.10 25.84 23.58 22.79 31.31 15.34 5.74 27.27 25.10 23.58 24.35 18.43 22.79 27.27 20.27 12.92 45.57 21.97 28.66 21.97 42.71 11.54 25.84 31.95 21.97 36.87 48.45 24.35 18.43 49.60 21.97 24.35 30.00 27.27 21.13 16.43 28.66 20.27 15.34 25.10 32.58 18.43 18.43 25.10 39.82 20.27 46.15 18.43 26.57 23.58 36.87 31.95 21.13 18.43 21.97 25.10 22.79 24.35 21.97 8.13 21.13 32.58 47.29 40.98 21.13 40.40 12.92 38.06 33.21 27.97 28.39 30.94 26.70 25.41 28.91 21.00 28.12 24.28 20.92

227.13 247.50 213.57 203.25 231.28 168.02 224.96 194.20 167.40 Faktor M. Ikan 0 3 6 Total 0 227.1306 247.4972 213.5654 688.1932 473609.9 Vit. E 100 203.2522 231.282 168.0229 602.5571 363075.1 200 224.9635 194.2035 167.397 586.5641 344057.4 Total 655.3464 672.9827 548.9853 429478.9 452905.7 301384.9

101

Lampiran 11. Analisis statistika pengaruh tingkat penggunaan minyak ikan dan suplementasi vitamin E dalam ransum terhadap limfosit

R1 R2 R3 R4 R5 R6 R7 R8 R9 89 72 81 81 84 85 72 90 97 79 81 83 80 87 84 79 86 94 45 84 76 83 53 95 81 71 82 56 39 83 89 42 86 81 72 78 87 89 77 88 88 82 71 90 90 80 57 86 48 90 79 84 61 72 82 90 86 82 85 83 86 95 87 69 44 54 87 58 94 62 70 78

73.375 69.5 78.25 79.75 73.375 86 77 79.375 84.75

R1 R2 R3 R4 R5 R6 R7 R8 R9 70.63 58.05 64.16 64.16 66.42 67.21 58.05 71.57 80.03 62.73 64.16 65.65 63.43 68.87 66.42 62.73 68.03 75.82 42.13 66.42 60.67 65.65 46.72 77.08 64.16 57.42 64.90 48.45 38.65 65.65 70.63 40.40 68.03 64.16 58.05 62.03 68.87 70.63 61.34 69.73 69.73 64.90 57.42 71.57 71.57 63.43 49.02 68.03 43.85 71.57 62.73 66.42 51.35 58.05 64.90 71.57 68.03 64.90 67.21 65.65 68.03 77.08 68.87 56.17 41.55 47.29 68.87 49.60 75.82 51.94 56.79 62.03 59.66 57.51 62.60 63.90 60.06 68.48 61.61 63.98 67.91 477.30 460.05 500.81 511.22 480.52 547.83 492.90 511.85 543.28

Faktor M. Ikan 0 3 6 Total 0 477.29543 460.05052 500.81465 1438.1606 2068305.9Vit. E 100 511.22074 480.51809 547.8339 1539.5727 2370284.2 200 492.90297 511.84922 543.28137 1548.0336 2396407.9 Total 1481.4191 1452.4178 1591.9299 2194602.7 2109517.5 2534240.8


Perlakuan 8 861.99142 107.74893 1.3161061 2.096 M. Ikan 2 451.62956 225.81478 2.7582289 3.146

Vit. E 2 311.50094 155.75047 1.9024239 3.146 M X E 4 98.860915 24.715229 0.3018857 2.526 Galat 63 5157.7777 81.869487 Total 71 6019.7691

M. Ikan Lymfosit Perbedaan Antar Perlakuan SSR 0.05 LSR 0.05 Signifikansi

6 66.33 a 0 61.73 4.60 2.83 5.22 ab 3 60.52 5.81 1.21 2.98 5.50 b

102

Lampiran 12. Koefisien variasi bobot awal ayam broiler umur 1 hari (DOC) penelitian tahap II

Kode 1 2 3 4 5 6 7 8 9 10 1.1. 47 50 50 50 52 50 53 49 42 53 1.2. 49 51 49 51 47 39 48 52 47 51 1.3 48 51 51 49 49 51 45 49 56 48 1.4 50 48 52 42 49 49 52 50 50 48 2.1 50 47 52 52 47 49 50 53 40 54 2.2 47 47 48 52 49 51 48 47 50 53 2.3 40 43 40 46 45 55 48 49 48 40 2.4 50 48 48 36 51 44 50 49 50 56 3.1 51 49 53 48 50 48 55 44 54 54 3.2 50 45 51 47 51 54 52 50 48 51 3.3 53 49 44 51 46 51 55 50 48 52 3.4 54 49 48 55 54 52 44 51 52 53 4.1 48 43 48 53 51 53 47 50 49 46 4.2 44 48 50 48 49 46 52 46 41 46 4.3 42 53 55 44 54 50 52 52 53 47 4.4 54 45 48 48 50 48 61 51 43 47 5.1 52 42 55 50 59 47 49 52 48 42 5.2 48 50 47 48 51 52 52 52 48 50 5.3 49 53 46 54 51 48 45 45 53 50

6.1 51 52 50 55 51 48 50 49 48 50 6.2 47 53 52 48 45 50 52 51 48 50 6.3 48 48 53 54 56 43 43 48 51 49 6.4 46 53 54 53 57 43 53 52 56 48 7.1 55 47 51 50 55 48 56 51 54 46 7.2 49 55 51 52 45 45 50 50 54 57 7.3 53 49 56 45 56 50 52 46 48 52 7.4 50 53 46 58 49 51 54 52 57 50 8.1 51 55 51 49 48 52 49 44 53 44 8.2 50 55 47 52 49 50 51 55 51 46 8.3 50 56 51 50 56 45 51 52 47 45

Jumlah Total 14909 Rataan 49.7 STDEV 3.72 KV 7.485 %

103

Lampiran 13. Analisis statistika pengaruh perlakuan terhadap konsumsi ransum sebelum dan sesudah uji tantang virus IBD atau ND

1 2 3 4 5 6 7 8 1187.90 1260.87 1180.45 1063.93 1135.61 1253.74 1147.62 1036.94 1119.20 1190.76 1119.04 1116.91 1268.08 1133.48 1183.31 1132.52 1192.08 1168.96 1140.23 1028.96 1112.31 1183.07 1109.68 1086.76 1169.97 1130.42 1085.26 1195.83 1181.01 1153.48 1167.29 1187.75 1131.24 1069.93 1177.96 1187.83 1148.52 1085.41Total 4669.14 4751.00 4524.97 3209.80 4711.84 4751.31 4594.09 3256.22


Perlakuan 7 48877.18993 6982.455705 3.055808578 2.46 Galat 22 50269.52 2284.978109 Total 29 99146.71 Perla kuan


SSR 0.05

LSR 0.05

Signi fikansi

6 1187.83 a 2 1187.75 0.08 2.93 70.03 a 4 1177.96 9.87 9.79 3.08 73.61 a 1 1167.29 20.54 20.46 10.67 3.17 75.77 a 7 1148.52 39.30 39.23 29.44 18.76 3.24 77.44 a 3 1131.24 56.58 56.51 46.72 36.04 17.28 3.29 78.63 ab 8 1085.41 102.42 102.34 92.55 81.88 63.11 45.84 3.32 79.35 b 5 1069.93 117.89 117.82 108.03 97.35 78.59 61.31 15.47 3.35 80.07 b

Umur 3 – 4 minggu (Sesudah ditantang virus IBD) 1 2 3 4 5 6 7 8

1 827.89 856.00 913.38 853.78 907.33 852.56 798.22 747.672 795.56 757.89 670.29 826.44 778.89 609.44 785.00 704.133 767.78 769.00 854.89 833.22 871.67 785.00 788.56 806.444 769.22 738.56 701.89 874.33 779.78 825.25

790.11 780.36 785.11 837.81 858.06 756.69 799.26 752.75Total 3160.44 3121.44 3140.44 2513.44 3432.22 3026.78 3197.03 2258.24


Perlakuan 7 34045.51867 4863.645524 1.070524676 2.46 Galat 22 99951.18 4543.235326 Total 29 133996.70

104

Umur 4 – 5 minggu (Sesudah uji tantang virus IBD atan ND) 1 2 3 4 5 6 7 8

1 857.66 875.46 888.47 899.52 755.76 738.20 927.39 840.222 891.17 903.45 1102.58 873.00 836.29 1037.88 901.24 795.413 895.35 878.53 866.37 921.86 891.79 903.20 844.27 915.854 915.60 917.32 757.85 744.71 921.99 865.39

889.94 893.69 903.82 898.13 807.14 900.32 884.57 850.49Total 3559.78 3574.75 3615.27 2694.39 3228.55 3601.27 3538.29 2551.48



Umur 5 – 6 minggu (Sesudah uji tantang virus IBD atau ND) 1 2 3 4 5 6 7 8

1 1050.68 1153.86 1065.02 999.46 1014.02 1048.86 1050.68 1114.322 978.19 992.75 989.89 1126.08 756.10 1144.26 1040.05 987.153 969.80 986.59 979.17 939.85 1015.84 1033.89 1049.28 1156.304 984.91 985.19 909.32 954.40 1053.48 1000.58

995.90 1029.60 985.85 1021.80 935.09 1070.12 1035.15 1085.92Total 3983.58 4118.38 3943.40 3065.39 3740.36 4280.49 4140.59 3257.77




1 2736.23 3007.78 2991.16 2752.76 2677.11 2639.62 2776.29 2702.212 2664.92 2654.08 3126.46 2947.65 2371.29 2791.58 2726.29 2597.963 2632.92 2634.12 2700.43 2694.93 2779.29 2722.09 2682.11 3006.714 2669.73 2641.07 2369.06 2573.45 2755.25 2691.22

2675.95 2734.26 2796.78 2798.45 2600.28 2727.14 2718.98 2768.96Total 10703.80 10937.05 11187.10 8395.35 10401.13 10908.54 10875.91 8306.87



105

Lampiran 14. Analisis statistika pengaruh perlakuan terhadap pertambahan bobot badan sebelum dan sesudah uji tantang virus IBD atau ND

Umur 1 – 3 minggu (Sebelum uji tantang virus IBD atau ND) 1 2 3 4 5 6 7 8 634.62 565.10 475.65 510.42 441.62 562.49 475.03 367.73 457.82 581.80 359.81 551.88 505.87 450.03 477.53 542.40 454.86 548.71 546.32 550.24 450.82 496.26 403.30 579.59 491.33 475.36 449.47 541.06 474.83 387.22 509.66 542.74 457.81 538.40 466.10 495.90 435.77 496.57Total 2038.63 2170.97 1831.25 2153.60 1398.31 1983.60 1743.09 1489.72



Umur 3 – 4 minggu (Sesudah uji tantang virus IBD)

1 2 3 4 5 6 7 8 434.53 407.13 462.13 424.56 452.74 434.11 324.78 327.33 336.90 397.22 286.43 414.67 439.77 320.38 371.89 285.75 403.11 386.56 463.56 433.89 478.32 356.33 336.78 402.33 353.11 372.67 281.44 420.37 340.44 344.90 381.91 390.89 373.39 423.37 456.94 362.82 344.59 338.47Total 1527.65 1563.57 1493.55 1693.49 1370.83 1451.26 1378.35 1015.42

Sumber db JK KT F F0.05


Umur 4 – 5 minggu (Sesudah uji tantang virus IBD atau ND)

1 2 3 4 5 6 7 8 515.13 560.66 500.77 425.72 350.52 470.88 476.64 358.46 522.00 448.65 427.02 524.95 469.36 474.29 480.40 481.82 488.08 429.58 308.72 515.26 487.12 671.74 456.35 435.63 441.06 490.90 326.39 484.65 508.97 467.88 491.57 482.45 390.73 487.65 435.67 531.47 470.32 425.30Total 1966.26 1929.80 1562.90 1950.58 819.88 2125.87 1881.26 1275.91

106

Sumber db JK KT Fhit F0.05 Perlakuan 7 53599.3226 7657.046079 1.859235685 2.46 Galat 22 90604.44 4118.383775 Total 29 144203.77

Umur 5 – 6 minggu (Sesudah uji tantang virus IBD atau ND)

1 2 3 4 5 6 7 8 476.41 634.55 367.46 524.75 264.74 470.70 447.39 386.21 409.30 468.98 372.83 293.51 331.78 529.21 420.66 457.60 397.54 489.75 305.79 269.20 332.45 302.95 460.73 482.31 439.21 504.45 160.50 358.49 429.08 443.86 430.62 524.43 301.64 361.49 309.66 432.99 443.16 442.04Total 1722.46 2097.73 1206.58 1445.95 928.97 1731.94 1772.64 1326.11



Perla kuan


SSR 0.05

LSR 0.05

Signi fikansi

2 524.43 a 7 443.16 81.27 2.93 111.15 ab 8 442.04 82.39 1.12 3.08 116.84 ab 6 432.99 91.45 10.18 9.05 3.17 120.25 abc 1 430.62 93.82 12.54 11.42 2.37 3.24 122.91 abc 4 361.49 162.94 81.67 80.55 71.50 69.13 3.29 124.81 bcd 5 309.66 214.78 133.50 132.38 123.33 120.96 51.83 3.32 125.95 cd 3 301.64 222.79 141.52 140.39 131.34 128.97 59.84 8.01 3.35 127.08 d

Umur 3 – 6 minggu (Sesudah uji tantang virus IBD atan ND)

1 2 3 4 5 6 7 8 1426.06 1602.33 1330.35 1374.03 1173.52 1375.68 1248.81 1072.00 1268.21 1314.86 1086.29 1229.13 1174.72 1323.88 1272.95 1225.17 1288.73 1305.89 1078.06 1217.35 1258.53 1331.02 1253.86 1320.28 1233.38 1368.02 768.33 1269.50 1278.50 1256.63 1304.10 1397.77 1065.76 1272.50 1202.26 1327.27 1258.06 1205.81Total 5216.38 5591.10 4263.03 5090.01 2348.24 5309.07 5032.25 3617.44



107

Perla kuan Perbedaan Antar Perlakuan

SSR 0.05

LSR 0.05

Signifi kansi

2 1397.77 a 6 1327.27 70.51 2.93 170.71 ab 1 1304.10 93.68 23.17 3.08 179.45 ab 4 1272.50 125.27 54.76 31.59 3.17 184.70 ab 7 1258.06 139.71 69.20 46.03 14.44 3.24 188.77 abc 8 1205.81 191.96 121.45 98.28 66.69 52.25 3.29 191.69 bc 5 1202.26 195.51 125.01 101.83 70.24 55.80 3.55 3.32 193.44 bc 3 1065.76 332.02 261.51 238.34 206.75 192.31 140.06 136.50 3.35 195.18 c

108

Lampiran 15. Analisis statistika pengaruh perlakuan terhadap konversi ransum sebelum dan sesudah uji tantang virus IBD atau ND

Umur 1 – 3 minggu (Sebelum uji tantang virus IBD atau ND) 1 2 3 4 5 6 7 8

1 1.74 2.05 2.24 2.17 2.03 2.05 2.18 2.48 2 2.21 1.89 2.73 2.01 2.12 2.27 2.24 1.91 3 2.36 1.97 1.91 2.06 1.85 2.17 2.44 1.73 4 2.17 2.16 2.17 2.02 2.24 2.63

2.12 2.02 2.26 2.08 2.01 2.18 2.37 2.04 Total 8.47 8.07 9.05 6.23 8.03 8.73 9.49 6.12



Umur 3 – 4 minggu (Sesudah uji tantang virus IBD) 1 2 3 4 5 6 7 8

1 1.91 2.10 1.98 2.01 2.01 1.96 2.46 2.28 2 2.36 1.91 2.34 2.01 1.69 1.90 2.11 2.46 3 1.90 1.99 1.84 1.92 1.99 2.20 2.34 2.00 4 2.18 1.98 2.49 1.83 2.29 2.39

2.09 1.995 2.16 1.98 1.88 2.089915 2.326 2.25 Total 8.35 7.98 8.65 5.94 7.52 8.36 9.30 6.75




1 1.66 1.78 2.02 2.12 1.71 1.57 1.95 2.34 2 1.71 2.01 3.43 1.90 2.39 2.19 2.11 1.88 3 1.83 2.05 2.81 1.79 1.91 1.34 2.34 2.40 4 2.08 1.87 2.32 1.53 1.81 2.39

1.82 1.93 2.65 1.94 1.89 1.73 2.20 2.21 Total 7.28 7.71 10.58 5.81 7.55 6.91 8.79 6.62

109

Sumber db JK KT Fhit F0.05 Perlakuan 7 2.410359715 0.344337102 3.107058981 2.46 Galat 22 2.44 0.110824128 Total 29 4.85

Perla kuan


SSR 0.05

LSR 0.05

Signifi kansi

3 2.65 a 8 2.21 0.44 2.93 0.49 ab 7 2.20 0.45 0.01 3.08 0.51 ab 4 1.94 0.71 0.27 0.26 3.17 0.53 b 2 1.93 0.72 0.28 0.27 0.01 3.24 0.54 b 5 1.89 0.76 0.32 0.31 0.05 0.04 3.29 0.55 b 1 1.82 0.83 0.39 0.38 0.12 0.11 0.07 3.32 0.55 b 6 1.73 0.92 0.48 0.47 0.21 0.20 0.16 0.09 3.35 0.56 b


1 2.21 1.82 2.90 1.91 3.00 2.23 2.35 2.89 2 2.39 2.12 2.66 3.85 2.99 2.16 2.47 2.16 3 2.44 2.01 3.20 3.50 3.04 3.41 2.28 2.40 4 2.24 1.95 5.67 3.24 2.46 2.25

2.32 1.98 3.61 3.09 3.07 2.56 2.338 2.48 Total 9.28 7.90 14.42 9.26 12.27 10.26 9.35 7.44



Perla kuan


SSR 0.05

LSR 0.05

Signifi kansi

3 3.61 a 4 3.09 0.52 2.93 0.96 ab 5 3.07 0.54 0.02 3.08 1.01 ab 6 2.56 1.04 0.52 0.50 3.17 1.04 bc 8 2.48 1.13 0.61 0.59 0.08 3.24 1.06 bc 7 2.34 1.27 0.75 0.73 0.23 0.14 3.29 1.07 bc 1 2.32 1.29 0.77 0.75 0.25 0.16 0.02 3.32 1.08 bc 2 1.98 1.63 1.11 1.09 0.59 0.50 0.36 0.34 3.35 1.09 c

110


1 1.92 1.88 2.25 2.00 2.31 1.92 2.22 2.52 2 2.10 2.02 2.88 2.40 2.06 2.11 2.14 2.12 3 2.04 2.02 2.50 2.22 2.25 2.05 2.14 2.28 4 2.16 1.93 3.08 2.05 2.16 2.14

2.06 1.96 2.68 2.21 2.16 2.06 2.161 2.31 Total 8.23 7.84 10.71 6.62 8.65 8.23 8.65 6.92



Perla kuan


SSR 0.05

LSR 0.05

Signifi kansi

3 2.68 a 8 2.31 0.37 2.93 0.26 b 5 2.21 0.47 0.10 3.08 0.28 bc 4 2.16 0.52 0.14 0.04 3.17 0.29 bc 7 2.16 0.52 0.14 0.05 0.00 3.24 0.29 bc 1 2.06 0.62 0.25 0.15 0.11 0.10 3.29 0.30 bc 6 2.06 0.62 0.25 0.15 0.11 0.10 0.00 3.32 0.30 bc 2 1.96 0.72 0.35 0.25 0.20 0.20 0.10 0.10 3.35 0.30 c

111

Lampiran 16. Analisis statistika pengaruh perlakuan terhadap berat relatif bursa Fabricius sebelum dan sesudah uji tantang virus IBD atau ND

Umur 2 minggu 1 2 3 4 5 6 7 8

1 0.0024 0.0027 0.0040 0.0035 0.0019 0.0014 0.0023 0.0011 2 0.0029 0.0024 0.0099 0.0035 0.0027 0.0038 0.0024 0.0026 3 0.0044 0.0029 0.0059 0.0013 0.0023 0.0017 0.0026 0.0013 4 0.0021 0.0029 0.0018 0.0033 0.0037 0.0032

0.0030 0.0027 0.0054 0.0029 0.0023 0.0026 0.0026 0.0017 Total 0.0118 0.0109 0.0216 0.0117 0.0068 0.0105 0.0106 0.0051


Perlakuan 7 3.122E-05 4.46E-06 1.975772 2.46 Galat 22 0.00 2.257E-06 Total 29 0.00

Umur 4 minggu (sesudah uji tantang virus IBD atau ND)

1 2 3 4 5 6 7 8 0.002 0.001 0.0034 0.0028 0.0034 0.002 0.0022 0.002 0.001 0.0007 0.0016 0.0009 0.0012 0.002 0.0024 0.002 5E-04 0.0019 0.0032 0.001 0.0019 0.003 0.0024 0.003 7E-04 0.0005 0.0005 0.0015 0.001 0.0028 1E-03 0.001 0.0022 0.0016 0.0022 0.002 0.0025 0.002 Total 0.004 0.0041 0.0088 0.0062 0.0065 0.009 0.0098 0.007


Perlakuan 7 1.0191E-05 1.4559E-06 2.274082 2.46 Galat 22 1.4085E-05 6.4022E-07 Total 29 2.4276E-05

Perla- kuan

Rataan


SSR 0.05

LSR 0.05

Signi fikan

7 0.0025 a 8 0.0025 1E-05 2.93 0.001 a 6 0.0023 0.0002 0.0002 3.08 0.001 ab 3 0.0022 0.0003 0.0003 5E-05 3.17 0.001 ab 5 0.0022 0.0003 0.0003 9E-05 4E-05 3.24 0.001 ab 4 0.0016 0.0009 0.0009 0.0007 0.0006 0.0006 3.29 0.001 ab 2 0.001 0.0014 0.0014 0.0012 0.0012 0.0011 0.00054 3.32 0.001 b 1 0.001 0.0015 0.0015 0.0013 0.0012 0.0012 0.00058 4E-05 3.35 0.001 b

112


1 2 3 4 5 6 7 8 6E-04 0.0007 0.003 0.0016 0.002 0.0005 0.0008 0.0008 5E-04 0.0008 0.002 0.0019 0.003 0.0007 0.0005 0.0005 7E-04 0.0006 0.001 0.004 0.003 0.0007 0.0009 0.0019 7E-04 0.0009 0.001 0.0035 0.0004 0.0005 6E-04 0.0008 0.002 0.0028 0.002 0.0005 0.0007 0.0011 Total 0.002 0.003 0.008 0.011 0.007 0.0022 0.0027 0.0032



Per-laku- an

Perbedaan antar Perlakuan

SSR 0.05

LSR 0.05

Signi fikan si

4 0.003 a 5 0.002 4E-04 2.93 0.0008 a 3 0.002 8E-04 4E-04 3.08 0.0009 ab 8 0.001 0.002 0.001 9E-04 3.17 0.0009 bc 2 8E-04 0.002 0.002 0.001 3E-04 3.24 0.0009 c 7 7E-04 0.002 0.002 0.001 4E-04 8E-05 3.29 0.0009 c 1 6E-04 0.002 0.002 0.001 5E-04 2E-04 8E-05 3.32 0.0010 c 6 5E-04 0.002 0.002 0.001 5E-04 2E-04 1E-04 6E-05 3.35 0.0010 c


1 2 3 4 5 6 7 8 0.0005 0.0005 0.0008 0.0005 0.0004 0.0004 0.00038 0.0003 0.0003 0.0008 0.0007 0.0011 0.0009 0.0005 0.00038 0.0005 0.0008 0.0006 0.0015 0.0009 0.0003 0.0006 0.00059 0.0006 0.0006 0.0006 0.0007 0.0006 0.0007 0.00074 0.0006 0.0006 0.0009 0.0008 0.0005 0.0005 0.00052 0.0005 Total 0.0022 0.0025 0.0036 0.0031 0.0016 0.0021 0.00209 0.0015



113

Lampiran 17. Analisis statistika pengaruh perlakuan terhadap berat relatif limpa sebelum dan sesudah uji tantang virus IBD atau ND

Umur 2 minggu

1 2 3 4 5 6 7 8 0.0009 0.0006 0.0009 0.001 0.0011 0.0003 0.00116 0.0015 0.0011 0.0009 0.0018 0.0006 0.0007 0.0019 0.00091 0.0007 0.0012 0.0015 0.0018 0.001 0.0011 0.0014 0.00113 0.0013 0.0004 0.0004 0.0004 0.0003 0.0007 0.00079 0.0009 0.0008 0.0012 0.0007 0.001 0.0011 0.001 0.0012 Total 0.0035 0.0033 0.0048 0.0029 0.0029 0.0043 0.00399 0.0035




1 2 3 4 5 6 7 8 0.0015 0.0012 0.0011 0.0013 0.0014 0.0011 0.00147 0.0015 0.0014 0.0011 0.0011 0.0011 0.001 0.0021 0.00165 0.002 0.0013 0.0013 0.0009 0.0008 0.0014 0.0018 0.00135 0.0012 0.002 0.0018 0.0011 0.0014 0.0013 0.0025 0.0016 0.0013 0.001 0.0012 0.0013 0.0015 0.00174 0.0015 Total 0.0062 0.0054 0.0042 0.0046 0.0039 0.0062 0.00697 0.0046




1 2 3 4 5 6 7 8 0.0014 0.0011 0.0028 0.0019 0.0011 0.0013 0.00124 0.0018 0.0007 0.001 0.0012 0.002 0.0018 0.0005 0.00133 0.0011 0.001 0.0008 0.0008 0.0019 0.0019 0.0009 0.00129 0.002 0.0009 0.0012 0.0011 0.0024 0.0014 0.00099 0.001 0.001 0.0015 0.002 0.0016 0.001 0.00121 0.0016 Total 0.004 0.0041 0.0059 0.0082 0.0048 0.0041 0.00486 0.0049

114

Sumber db JK KT Fhit F0.05 Perlakuan 7 3.8218E-06 5.4597E-07 2.841351 2.46Galat 22 4.2273E-06 1.9215E-07 Total 29 8.0491E-06

Per laku- an

Rata- an


SSR 0.05

LSR 0.05

4 0.002 a 8 0.0016 0.0004 2.93 6E-04 ab 5 0.0016 0.0005 3E-05 3.08 7E-04 ab 3 0.0015 0.0006 0.0001 0.0001 3.17 7E-04 ab 7 0.0012 0.0008 0.0004 0.0004 0.0003 3.24 7E-04 b 6 0.001 0.001 0.0006 0.0006 0.0004 0.0002 3.29 7E-04 b 2 0.001 0.001 0.0006 0.0006 0.0005 0.0002 1.5E-05 3.32 7E-04 b 1 0.001 0.0011 0.0006 0.0006 0.0005 0.0002 4E-05 3E-05 3.35 7E-04 b


1 2 3 4 5 6 7 8 0.0015 0.0011 0.0008 0.0019 0.0007 0.0022 0.00306 0.0011 0.0007 0.0009 0.0019 0.0025 0.0012 0.0009 0.00383 0.0009 0.0013 0.0009 0.0013 0.0023 0.0015 0.0009 0.00118 0.0013 0.0007 0.0009 0.0017 0.0028 0.0024 0.00125 0.001 0.0009 0.0015 0.0024 0.0011 0.0016 0.00233 0.0011 Total 0.0042 0.0037 0.0058 0.0095 0.0034 0.0063 0.00932 0.0034

Sumber db JK KT F F0.05

Perlakuan 7 8.8471E-06 1.2639E-06 2.997873 Galat 22 9.2749E-06 4.2159E-07 Total 29 1.8122E-05

Per laku an

Rata-an


SSR 0.05

LSR 0.05

Signi- fikansi

4 0.0024 a 7 0.0023 5E-05 2.93 1E-03 a 6 0.0016 0.0008 0.0007 3.08 1E-03 a 3 0.0015 0.0009 0.0009 0.0001 3.17 0.001 ab 8 0.0011 0.0013 0.0012 0.0005 0.0003 3.24 0.001 b 5 0.0011 0.0013 0.0012 0.0005 0.0003 2E-06 3.29 0.001 b 1 0.001 0.0013 0.0013 0.0005 0.0004 8E-05 7.5E-05 3.32 0.001 b 2 0.0009 0.0015 0.0014 0.0007 0.0005 0.0002 0.0002 0.0001 3.35 0.001 b

115

Lampiran 18. Analisis statistika pengaruh perlakuan terhadap berat relatif timus sebelum dan sesudah uji tantang virus IBD atau ND

Umur 2 minggu

1 2 3 4 5 6 7 8 0.0067 0.0038 0.0044 0.0067 0.0004 0.0003 0.0027 0.0023 0.0032 0.0024 0.0018 0.001 0.0013 0.0057 0.00121 0.0013 0.006 0.0035 0.0011 0.0027 0.0011 0.0003 0.00528 0.0039 0.0068 0.0043 0.0028 0.003 0.0037 0.00238 0.0057 0.0035 0.0025 0.0033 0.0009 0.0025 0.00289 0.0025 Total 0.0227 0.0141 0.0101 0.0133 0.0028 0.01 0.01158 0.0075



Umur 4 minggu (sesudah uji tantang virus IBD) 1 2 3 4 5 6 7 8 0.0028 0.0018 0.0049 0.0078 0.006 0.0057 0.00543 0.0055 0.0023 0.0056 0.0023 0.006 0.0048 0.004 0.00236 0.0021 0.0031 0.0032 0.0027 0.0045 0.007 0.0039 0.00515 0.0034 0.0047 0.0038 0.0031 0.0048 0.005 0.00125 0.0032 0.0036 0.0033 0.0058 0.0059 0.0047 0.00355 0.0037 Total 0.0129 0.0145 0.013 0.0231 0.0178 0.0186 0.01419 0.011




1 2 3 4 5 6 7 8 0.0045 0.0064 0.0054 0.0019 0.0041 0.002 0.00186 0.0046 0.0026 0.0056 0.0034 0.0046 0.0042 0.0038 0.00357 0.0032 0.0064 0.0009 0.0012 0.0045 0.0049 0.0038 0.00576 0.0038 0.0063 0.0018 0.0028 0.0055 0.0054 0.00323 0.005 0.0037 0.0032 0.0041 0.0044 0.0037 0.00361 0.0039 Total 0.0199 0.0146 0.0129 0.0165 0.0132 0.015 0.01443 0.0116

116

Sumber db JK KT Fhit F0.05 Perlakuan 7 8.0435E-06 1.1491E-06 0.400703 2.46 Galat 22 6.3088E-05 2.8676E-06


1 2 3 4 5 6 7 8 0.0034 0.0074 0.0023 0.0032 0.0038 0.0026 0.00344 0.0045 0.0015 0.0047 0.0049 0.0041 0.0023 0.0037 0.00274 0.0032 0.0031 0.0038 0.0022 0.0026 0.003 0.005 0.00561 0.0053 0.0033 0.0057 0.0037 0.0013 0.0053 0.00453 0.0028 0.0054 0.0033 0.0028 0.003 0.0042 0.00408 0.0044 Total 0.0112 0.0216 0.0131 0.0112 0.0091 0.0167 0.01632 0.0131


Perlakuan 7 2.2958E-05 3.2797E-06 2.309567 2.46Galat 22 3.1241E-05 1.4201E-06 Total 29 5.4200E-05

117

Lampiran 19. Tehnik pembuatan preparat histopatologi.

Sampel organ diambil dan diawetkan

Sampel dipotong dengan ketebalan ± 3mm Dan difiksasi BNF 10%

Organ kembali dipotong dengan ukuran panjang ± 4mm,lebar 3mm dan tebal 3mm

Pengambilan air dari jaringan (dehidrasi): Organ-organ didehidrasi dengan alkohol konsentrasi bertingkat mulai dar 70%, 80%,

90%, 95% absolut 1 dan absolut 2. Masing-masing konsentrasi membutuhkan waktu ± 2 menit

Tahap penjernihan : Organ dimasukan ke dalam xylol selama 30 menit

Penanaman dalam parafin (embedding dan blok) : Organ-organ tersebut ditanam ke dalam parafin cair dalam lemari pemanas selama

2 jam dan kemudian organ-organ dimasukan ke dalam blok pencetak

Blok parafin dibekukan kemudian disimpan dalam lemari es

Blok dipotong dengan menggunakan mikrotom dengan ketebalan 5 µm (agar hasil potongan tidak mengkerut maka potongan diletakan diatas permukaan air bersuhu

45 oC), hasil potongan kemudian diangkat dengan gelas objek.

Gelas objek tersebut disimpan dan dikeringkan ke dalam inkubator dengan suhu 60 oC selama satu malam

Pewarnaan

118

Lampiran 20. Pewarnaan preparat histologi

Gelas objek yang telah diinkubator dimasukkan ke dalam alkohol konsentrasi bertingkat mulai dari absolut 1, absolut 2, 95%, 90%, 80%, 70% masing-masing

selama 2 menit

Lalu bilas dengan air mengalir

Pewarnaan hematoksilin, dilakukan dengan memasukan sediaan ke dalam zat

warna hematoksilin selama 2 – 3 menit

Sebelum dimasukkan ke dalam pewarnaan eosin harus dibilas terlebih dahulu dengan air mengalir, demikian pula setelah pewarnaan eosin sediaan kembali

dibilas dengan air

Sediaan dimasukkan ke dalam alkohol konsentrasi bertingkat

dan alkohol absolut 1 dan 2

Penjernihan : proses ini dilakukan dengan memasukan sediaan ke dalam xylol 1 dan

xylol 2 selama beberapa menit

Lalu dikeringkan di udara terbuka agar sediaan terhindar dari pengaruh lingkungan

Kemudian ditutup dengan cover glass yang direkatkan dengan zat perekat entelen

Sediaan kemudian dikeringkan di udara terbuka dan diberi label

agar mudah diidentifikasi

Pemeriksaan mikroskopik dilakukan dengan pembesaran 400 x

Perubahan organ pada masing-masing unit perlakuan diamati dengan pengamatan lima lapang pandang

119

Lampiran 21. Skor lesio histopatologi bursa fabricius Umur (minggu) 2 4 5 6 1 0 1 1 1 R9. Non Vaksin 2 0 1 1 1 3 0 1 1 1 4 0 1 1 1 Rataan 0 1 1 1 1 0 1 3 2 R9. Vaksin 2 0 1 1 0 3 0 1 0 1 4 0 0 1 Rataan 0 1 1 1 1 0 2 3 3 R9. Non Vaksin ND + infeksi ND 2 0 1 3 2 3 0 1 2 1 4 0 0 1 2 Rataan 0 1 2.25 2 1 0 0 2 3 R9. Vaksin + infeksi ND 2 0 0 3 2 3 0 1 1 1 4 0 1 1 2 Rataan 0 0.5 1.75 2 1 0 1 2 2 R0. Vaksin + infeksi ND 2 0 1 3 3 3 0 2 1 3 4 0 Rataan 0 1.33 2.0 2.67 1 0 2 3 3 R9. Non Vaksin IBD + infeksi IBD 2 0 2 2 2 3 0 2 2 2 4 0 2 3 1 Rataan 0 2 2.5 2 1 0 1 1 1 R9. Vaksin + infeksi IBD 2 0 0 1 1 3 0 1 2 1 4 0 1 2 1 Rataan 0 0.75 1.5 1 1 0 2 1 2 R0. Vaksin + infeksi IBD 2 0 2 1 2 3 0 1 1 1 4 0 Rataan 0 1.67 1 1.67

Keterangan : 0 = 0 – 0.25; 1 = 0.26 – 1.25; 2 = 1.26 – 2.25; 3 = 2.26 – 3.00

120

Lampiran 21. Skor lesio histopatologi bursa Fabricius Umur (minggu) 2 4 5 6 R9. Non Vaksin 0 1.00 1.00 1.00 R9. Vaksin 0 1.00 1.00 1.00 R9. Non Vaksin ND + infeksi ND 0 1.00 2.25 2.00 R9. Vaksin + infeksi ND 0 0.50 1.75 2.00 R0. Vaksin + infeksi ND 0 1.33 2.00 2.67 R9. Non Vaksin IBD + infeksi IBD 0 2.00 2.50 2.00 R9. Vaksin + infeksi IBD 0 0.75 1.50 1.00 R0. Vaksin + infeksi IBD 0 1.67 1.00 1.67 Uji Kruskal-Walis skor lesio histipatologi bursa Fabricius umur 4 minggu 1 2 3 4 5 6 7 8 13 13 25.5 2.5 13 25.5 13 25.5 13 13 13 2.5 13 25.5 2.5 25.5 13 13 13 13 25.5 25.5 13 13 13 2.5 13 25.5 13 Ri 52 39 54 31 51.5 102 41.5 64 Ri

2 2704 1521 2916 961 2652.25 10404 1722.25 4096 Ri

2/ni 676 507 729 240.25 884.0833 2601 430.5625 1365.333 H = [12/(29 x 30)] x Σi Ri

2/ni – 3 x 30 H = [12/(29 x 30)] x 7433.229– 3 x 30 H = 12.5273 Hterkoreksi = H/ {(1 – [Σ T/(n-1)n(n+1)]} Hterkoreksi = 12.5273/0.797553 Hterkoreksi = 15.70717; χ2

0.05; db 7 = 14.1; Hterkoreksi > χ20.05; db 7

Uji jarak berganda Duncan skor lesio histipatologi bursa Fabricius umur 4 minggu Perlakuan

Perbedaan Antar perlakuan

SSR 0.05

LSR 0.05

Signifi-kansi

6 2 a 8 1.67 0.33 2.93 0.75 ab 5 1.33 0.67 0.33 3.08 0.79 abc 2 1 1.00 0.67 0.33 3.17 0.82 bc 1 1 1.00 0.67 0.33 0.00 3.24 0.83 bc 3 1 1.00 0.67 0.33 0.00 0.00 3.29 0.85 bc 7 0.75 1.25 0.92 0.58 0.25 0.25 0.25 3.32 0.85 c 4 0.50 1.50 1.17 0.83 0.50 0.50 0.50 0.25 3.35 0.86 c

121

Uji Kruskal-Walis skor lesio histipatologi bursa Fabricius umur 5 minggu 1 2 3 4 5 6 7 8 9.5 27 27 20 20 27 9.5 9.5 9.5 9.5 27 27 27 20 9.5 9.5 9.5 1.5 20 9.5 9.5 20 20 9.5 9.5 1.5 9.5 9.5 27 20

Ri 38 39.5 83.5 66 56.5 94 59 28.5 Ri

2 1444 1560.25 6972.25 4356 3192.25 8836 3481 812.25 Ri

2/ni 361 390.0625 1743.063 1089 1064.083 2209 870.25 270.75 H = [12/(30 x 31)] x Σi Ri

2/ni – 3 x 31 H = [12/(30 x 31)] x 7997.208 – 3 x 31 H = 10.18978 Hterkoreksi = H/ {(1 – [Σ T/(n-1)n(n+1)]} Hterkoreksi = 10.18978/0.873637 Hterkoreksi = 11.66363; χ2

0.05; db 7 = 14.1; Hterkoreksi < χ20.05; db 7

Uji Kruskal-Walis skor lesio histipatologi bursa Fabricius umur 6 minggu

1 2 3 4 5 6 7 8 8 20 28 28 20 28 8 20 8 1 20 20 28 20 8 20 8 8 8 8 28 20 8 20 8 8 20 20 8 8

Ri 32 37 76 76 76 76 32 60 Ri

2 1024 1369 5776 5776 5776 5776 1024 3600 Ri

2/ni 256 342.25 1444 1444 1925.333 1444 256 1200 H = [12/(30 x 31)] x Σi Ri

2/ni – 3 x 31 H = [12/(30 x 31)] x 8311.583 – 3 x 31 H = 14.24624 Hterkoreksi = H/ {1 – [Σ T/(n-1)n(n+1)]} Hterkoreksi = 14.24624/0.865628 Hterkoreksi = 16.45768; χ2


Uji jarak berganda Duncan skor lesio histipatologi bursa Fabricius umur 6 minggu Perlaku- an Perbedaan Antar Perlakuan

SSR 0.05

LSR 0.05

Signifi-kansi

5 2.67 a 3 2 0.67 2.93 0.9 a 4 2 0.67 0 3.08 1.0 a 6 2 0.67 0 0 3.17 1.0 a 8 2 0.67 0 0 0 3.24 1.0 a 7 1 1.67 1 1 1 1 3.29 1.0 b 1 1 1.67 1 1 1 1 0 3.32 1.0 b 2 1 1.67 1 1 1 1 0 0 3.35 1.1 b

122

Lampiran 22. Skor lesio histopatologi limpa Umur(minggu) 2 4 5 6 1 0 0 0 2 R9. Non Vaksin 2 1 1 1 3 3 1 1 0 1 4 1 1 1 1 Rataan 0.75 0.75 0.50 1.75 1 0 0 1 1 R9. Vaksin 2 0 0 0 1 3 0 0 0 1 4 1 0 0 1 Rataan 0.25 0 0.25 1 1 0 0 2 2 R9. Non Vaksin ND + infeksi ND 2 0 1 2 3 3 0 1 2 2 4 0 0 2 2 Rataan 0 0.5 2 2.25 1 0 0 2 1 R9. Vaksin + infeksi ND 2 1 0 2 1 3 0 0 2 1 4 0 0 2 1 Rataan 0.25 0 2 1 1 0 0 3 3 R0. Vaksin + infeksi ND 2 0 0 3 3 3 0 0 3 3 4 0 Rataan 0 0 3.0 3.0 1 0 1 2 2 R9. Non Vaksin IBD + infeksi IBD 2 0 1 2 2 3 1 1 2 1 4 1 0 1 1 Rataan 0.5 0.75 1.75 1.50 1 0 0 2 2 R9. Vaksin + infeksi IBD 2 0 0 2 1 3 0 0 1 1 4 0 0 1 1 Rataan 0 0 1.50 1.25 1 0 1 1 2 R0. Vaksin + infeksi IBD 2 0 0 1 1 3 0 0 2 1 4 0 Rataan 0 0.33 1.33 1.33 Keterangan : 0 = 0 – 0.25; 1 = 0.26 – 1.25; 2 = 1.26 – 2.25; 3 = 2.26 – 3.00

123

Lampiran 22. Skor lesio histopatologi limpa

Umur

(minggu) 2 4 5 6 R9 non vaksin 0 0.50 0.50 0.45 R9 vaksin 0 0.87 0.83 0.38 R9 non vaksin ND+tantang ND 0 0.38 2.00 1.85 R9 vaksin + tantang ND 0 0.63 0.75 0.85 R0 vaksin + tantang ND 0 0.63 2.10 1.97 R9 non vaksin IBD+tantang IBD 0 0.70 0.75 1.60 R9 vaksin+tantang IBD 0 0.68 0.53 0.80 R9 vaksin+tantang IBD 0 0.67 1.03 1.73

Uji kruskal walis skor lesio limpa umur 4 mg 1 2 3 4 5 6 7 8 9.5 28.5 9.5 22.5 9.5 26 4 4 9.5 26 1 22.5 22.5 15.5 15.5 15.5 4 19.5 9.5 9.5 15.5 15.5 26 28.5 15.5 4 4 19.5 22.5

Ri 38.5 74 24 58.5 47.5 76.5 68 48 Ri

2 1482.25 5476 576 3422.25 2256.25 5852.25 4624 2304 Ri

2/ni 370.5625 1825.333 144 855.5625 752.0833 1463.063 1156 768 H = [12/(30 x 31)] x Σi Ri

2/ni – 3 x 31 H = [12/(30 x 31)] x7334.604 – 3 x 31 H = 8.166954 χ2

0.05; db 7 = 14.1; H > χ20.05; db 7

Uji kruskal walis skor lesio limpa umur 5 mg 1 2 3 4 5 6 7 8 3.50 12.50 24.00 26.00 28.00 12.50 19.00 21.00 6.00 25.00 28.00 1.50 21.00 16.50 6.00 23.00 9.50 6.00 21.00 9.50 28.00 12.50 3.50 16.50 12.50 16.50 30.00 1.50 16.50 8.00

Ri 31.5 60 103 38.5 77 58 36.5 60.5 Ri

2 992.25 3600 10609 1482.25 5929 3364 1332.25 3660.25 Ri

2/ni 248.0625 900 2652.25 370.5625 1976.333 841 333.0625 1220.083H = [12/(30 x 31)] x Σi Ri

2/ni – 3 x 31 H = [12/(30 x 31)] x 8541.354 – 3 x 31 H = 17.21102 χ2

0.05; db 7 = 14.1; H> χ20.05; db 7

124

Uji jarak berganda Duncan skor lesio limpa umur 5 minggu Perlakuan


SSR0.05

LSR0.05

Signifi- kansi

5 2.10 a 3 2.00 0.10 2.93 0.8 ab 8 1.03 1.07 0.97 3.08 0.9 bc 2 0.83 1.28 1.18 0.21 3.17 0.9 c 4 0.75 1.35 1.25 0.28 0.08 3.24 0.9 c 6 0.75 1.35 1.25 0.28 0.08 0.00 3.29 0.9 c 7 0.53 1.58 1.48 0.51 0.30 0.23 0.23 3.32 1 c 1 0.50 1.60 1.50 0.53 0.33 0.25 0.25 0.03 3.35 1 c

Uji kruskal walis skor lesio limpa umur 6 mg 1 2 3 4 5 6 7 8 1.50 11.50 5.50 9.00 30.00 11.50 3.50 24.00 15.00 3.50 29.00 18.00 23.00 27.50 20.00 21.00 11.50 7.50 25.00 7.50 17.00 27.50 15.00 22.00 1.50 5.50 26.00 19.00 15.00 11.50

Ri 29.5 28 85.5 53.5 70 81.5 50 67 Ri

2 870.25 784 7310.25 2862.25 4900 6642.25 2500 4489 Ri

2/ni 217.5625 196 1827.563 715.5625 1633.333 1660.563 625 1496.333H = [12/(30 x 31)] x Σi Ri

2/ni – 3 x 31 H = [12/(30 x 31)] x 8371.917 – 3 x 31 H = 15.02473 χ2

0.05; db 7 = 14.1; H > χ20.05; db 7

Uji jarak berganda Duncan skor lesio limpa umur 6 minggu Perlalakuan


SSR0.05

LSR0.05

Signifi- kansi

5 1.97 a 3 1.85 0.12 2.93 0.99 ab 8 1.73 0.24 0.12 3.08 1.04 ab 6 1.60 0.37 0.25 0.13 3.17 1.07 abc 4 0.85 1.12 1.00 0.88 0.75 3.24 1.09 bc 7 0.80 1.17 1.05 0.93 0.80 0.05 3.29 1.11 bc 1 0.45 1.52 1.40 1.28 1.15 0.40 0.35 3.32 1.12 c 2 0.38 1.60 1.48 1.36 1.23 0.48 0.43 0.08 3.35 1.13 c

125

Lampiran 23. Skor lesio histopatologi timus Umur (minggu) 2 4 5 6 1 0 1 1 1 R9. Non Vaksin 2 1 0 1 1 3 1 2 1 3 4 0 2 0 2 Rataan 0.50 1.25 0.75 1.75 1 0 1 1 1 R9. Vaksin 2 0 1 0 1 3 1 0 0 1 4 0 1 Rataan 0.33 0.67 0.25 1.00 1 0 2 1 3 R9. Non Vaksin ND + infeksi ND 2 1 1 1 3 3 0 0 1 3 4 1 2 0 3 Rataan 0.50 1.25 0.75 3.00 1 1 1 1 1 R9. Vaksin + infeksi ND 2 0 0 1 2 3 0 0 2 2 4 0 0 1 2 Rataan 0.25 0.25 1.25 1.75 1 0 0 1 3 R0. Vaksin + infeksi ND 2 0 0 1 2 3 1 1 1 1 4 Rataan 0.33 0.33 1.00 2.00 1 0 1 2 2 R9. Non Vaksin IBD + infeksi IBD 2 0 1 2 2 3 1 0 1 3 4 1 0 1 2 Rataan 0.50 0.50 1.50 2.25 1 1 0 1 2 R9. Vaksin + infeksi IBD 2 0 1 1 1 3 0 0 2 2 4 0 2 1 Rataan 0.33 0.25 1.50 1.50 1 0 1 2 3 R0. Vaksin + infeksi IBD 2 0 2 2 2 3 1 1 2 2 4 Rataan 0.33 1.33 2.00 2.33

126

Lampiran 23. Skor lesio histopatologi timus Umur (minggu) 2 4 5 6 R9. Non Vaksin 0.50 1.25 0.75 1.75 R9. Vaksin 0.33 0.67 0.25 1.00 R9. Non Vaksin ND + infeksi ND 0.50 1.25 0.75 3.00 R9. Vaksin + infeksi ND 0.25 0.25 1.25 1.75 R0. Vaksin + infeksi ND 0.33 0.33 1.00 2.00 R9. Non Vaksin IBD + infeksi IBD 0.5 0.50 1.50 2.25 R9. Vaksin + infeksi IBD 0.33 0.25 1.50 1.50 R0. Vaksin + infeksi IBD 0.33 1.33 2.00 2.33 Uji Kruskal-Walis skor lesio histipatologi timus umur 2 minggu 1 2 3 4 5 6 7 8 9 9 9 23 9 9 23 9 23 9 23 9 9 9 9 9 23 23 9 9 23 23 9 23 9 23 9 23

Ri 64 41 64 50 41 64 41 41Ri

2 4096 1681 4096 2500 1681 4096 1681 1681Ri

2/ni 1024 560.3333 1024 625 560.3333 1024 560.333 560.333H = [12/(28 x 29)] x Σi Ri

2/ni – 3 x 29 H = [12/(28 x 29)]x 5938.333 - 3 x 29 H = 0.758621 Hterkoreksi = H/ {1 – [Σ T/(n-1)n(n+1)]} Hterkoreksi = 0.758621/0.716475 Hterkoreksi =1.058824; χ2


Uji Kruskal-Walis skor lesio histipatologi timus umur 4 minggu 1 2 3 4 5 6 7 8 19 19 27 19 7 19 7 19 7 19 19 7 7 19 19 27 27 7 7 7 19 7 7 19 27 27 7 7 7

Ri 80 45 80 40 33 52 40 65Ri

2 6400 2025 6400 1600 1089 2704 1600 4225Ri

2/ni 1600 675 1600 400 363 676 400 1408.333H = [12/(29 x 30)] x Σi Ri

2/ni – 3 x 30 H = [12/(29 x 30)] x7122.333 – 3 x 30 H = 8.23908 Hterkoreksi = H/ {1 – [Σ T/(n-1)n(n+1)]} Hterkoreksi = 8.23908/0.851232 Hterkoreksi =9.679012; χ2


127

Uji Kruskal-Walis skor lesio histipatologi timus umur 5 minggu 1 2 3 4 5 6 7 8 14 14 14 14 14 26.5 14 26.5 14 3 14 14 14 26.5 14 26.5 14 3 14 26.5 14 14 26.5 26.5 3 3 3 14 14 26.5

Ri 45 23 45 68.5 42 81 81 79.5Ri

2 2025 529 2025 4692.25 1764 6561 6561 6320.25Ri

2/ni 506.25 132.25 506.25 1173.06 588 1640.25 1640.25 2106.75H = [12/(30 x 31)] x Σi Ri

2/ni – 3 x 31 H = [12/(30 x 31)] x 8293.063 – 3 x 31 H = 14.00726 Hterkoreksi = H/ {1 – [Σ T/(n-1)n(n+1)]} Hterkoreksi = 14.00726/0.795328 Hterkoreksi =17.61192; χ2


Uji jarak berganda Duncan skor lesio histipatologi timus umur 5 minggu Perla- kuan

Ra- taan


SSR 0.05

LSR 0.05

Signifi- kansi

8 2 a 6 1.5 0.5 2.93 0.70 ab 7 1.5 0.5 0 3.08 0.73 ab 4 1.25 0.75 0.25 0.25 3.17 0.76 ab 5 1 1 0.5 0.5 0.25 3.24 0.77 bc 1 0.75 1.25 0.75 0.75 0.5 0.25 3.29 0.78 bc 3 0.75 1.25 0.75 0.75 0.5 0.25 0 3.32 0.79 bc 2 0.25 1.75 1.25 1.25 1 0.75 0.5 0.5 3.35 0.80 c

Uji Kruskal-Walis skor lesio histipatologi timus umur 6 minggu 1 2 3 4 5 6 7 8 5.5 5.5 27 17 5.5 17 17 27 5.5 5.5 27 17 17 17 5.5 17 27 5.5 27 17 5.5 27 17 17 17 5.5 27 17 17 5.5

Ri 55 22 108 68 28 78 45 61Ri

2 3025 484 11664 4624 784 6084 2025 3721Ri

2/ni 756.25 121 2916 1156 261.333 1521 506.25 1240.33H = [12/(30 x 31)] x Σi Ri

2/ni – 3 x 31 H = [12/(30 x 31)] x 8478.167 – 3 x 31 H = 16.3957 Hterkoreksi = H/ {1 – [Σ T/(n-1)n(n+1)]} Hterkoreksi = 16.3957/0.869855 Hterkoreksi =18.84876; χ2


128

Uji jarak berganda Duncan skor lesio histipatologi timus umur 6 minggu Perla- kuan

Rata- an


SSR 0.05

LSR 0.05 Signifi-

3 3.00 a 8 2.33 0.67 2.93 0.8788 ab 6 2.25 0.75 0.08 3.08 0.9238 ab 5 2.00 1.00 0.33 0.25 3.17 0.9508 b 4 1.75 1.25 0.58 0.50 0.25 3.24 0.9718 bc 1 1.75 1.25 0.58 0.50 0.25 0.00 3.29 0.9868 bc 7 1.50 1.50 0.83 0.75 0.50 0.25 0.25 3.32 0.9958 bc 2 1.00 2.00 1.33 1.25 1.00 0.75 0.75 0.50 3.35 1.0048 c

Documents

Dapus 2008dru-8.pdf