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Factors affecting on semen quality

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Factors affecting on semen quality. ผศ.น.สพ.ชัยวัฒน์ จรัสแสง ภาควิชาศัลยศาสตร์และวิทยาการสืบพันธ ุ์. ให้นักศึกษาอธิบายปัจจัยที่ทำให้เชื้ออสุจิตายมา 1 ตัวอย่าง. References. - PowerPoint PPT Presentation

Text of Factors affecting on semen quality

  • Factors affecting on semen quality...

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  • ReferencesBarth A.D and Waldner C.L. 2002. Factors affecting breeding soundness classification of beef bulls examined at the Western College of Veterinary Medicine. Can Vet J 43(3):274-284Hafez.E.S.E and Hafez.B. 2000. Reproduction in farm animals 7 th edition. Lippincott Williams & Wilkins. 509pp http://vet.kku.ac.th/chaiwat/teaching/teaching2010

  • Objectives

  • Breeding soundness evaluation

    Pedigree

    Progeny test - Long time - More costly

  • Breeding soundness evaluation

    Breeding Index

    (USA TPI=Type production index)(Canada LPI= Lifetime profit index)(New Zealand BVS = Breeding values)(Japan NTP=Nippon total index)

  • Terminology of semenPTA (ETA) : Predicted or estimated transmitting ability EBV : Estimated breeding value (Canada)PTAT : Predicted transmitting ability of type traitsReliability

  • Factors on Semen qualityBulls effectFeeding DiseasesSemen collection managementEnvironmental effects

  • Bulls affecting Age Scrotal circumference Breeds Body condition score Physical abnormalities Frostbite ???

  • Effect of Age

  • Effect of Breeds

  • Effect of BCS

  • Effect of Physical abnormalities

  • Effect of Scrotal circumferenceMinimum Recommended Scrotal Circumference in Centimeters by Age for Bulls Age (months) Scrotal Circumference (cm) 15 30>15-18 31>18-21 32>21-24 33>24 34

  • Effect of scrotal frostbite

  • - Puberty --> scrotal circumference - semen collection time- Bull > 6 years- Boar > 3 years- Stallion > 20 years Feeding on semen quality

  • Feeding

    NRC (National Research Council)

    Young animals - Low energy --> delay semen production- Protein --> less affecting- High energy ---> low libido

  • Thailand --> Late puberty

    - roughage- climates

  • Low feeding

    - Testosterone --> decrease- low libido- Obesity --> low libido and sexual activity

  • Vitamin A

    Pituitary gonadotropins

    Testicular degenerationVitamin E ---> Male organVitamins

  • Minerals

    - Iodine --> poor libido- Copper- Cobalt- Zinc- Manganese

  • Toxins - High gossipol --> epididymal cell

    - radiation --> spermatogenesis

    - estrogen feed ---> failure of sperm transport in the female tract

  • Diseases- Virus (IBR, BVD, FMD)

    - Bacteria (Brucellosis)

    - Parasite (Blood parasites)

  • Semen collection management

    - Training- Frequency 1-2 per week- hoof problem--> electro-ejaculator

  • Environmental effects1. Temperature- Motility - Temperature shock- Heat shock- Cold shock2. Photoperiod & seasonLong time --> Longevity sperm

    3. Oxygen ionO2 ---> H2O2

  • 4. AciditypH 6.7- 6.9Sperm metabolism --> Lactic acids ---> Buffers5. Osmostic pressurePermeabiliity 6. Metal Lead, Mercury7. Bacteria 8. Detergent & Toxin

  • 1. Semen collection

    2. Storage and freezing

    3. Storage and transportation

    4. Thawing and inseminationRisk of environment factors

  • Bulls prepare- Shower- Wash prepuce with NSS 0.9 %Material prepare- Detergent- SterileCollection time- Light- TemperatureSemen collection

  • Storage and freezing1. Freezing 37 C 5 C -20 C - 79 C -196 C

  • 2. Extender

    - protection cold shock and cryo-protectant- Energy source- Buffering- Osmotic pressure- Bacteria inhibition

  • Extender Egg-Yolk Tris

    - Tris ---> buffers- citric acid --> buffers- Glucose --> energy, osmotic pressure- Penicillin, Streptomycin, Polymixin B - Glycerol --> cryo- protectant- Egg-yolk --> cyro-protectant

  • Storage and Transport

    Semen bank tank- coding- liquid nitrogenAI center tank- liquid nitrogen--> 2 weeksInseminator tank- liquid nitrogen --> day by day

  • Transports- Liquid nitrogen- Tank to tank - Canister - Straw (0.25 French mini-straw 0.5 ml USA)- < 15 second- forceps

  • Thawing and InseminationThawing- canister level- forceps- 35-40 C 30 second

    AI- AI gun --> clean & dry

  • AI technique- Sperm death 10 -20 %

    - Live sperm 6- 8 m. / straw

    Progressive motile drop 5-15 %

    Limited of bulls

    Health control

  • ???