The world's first non-porous high resolution ODS column HPLC COLUMN - IMTAKT

Particle Size : 2umPore Size : Non-porous

Purified Silica

Changing polymer separation history

Go beyond the limitations of porous material; Try Presto !

polyethylene glycol (ca.20 kDa)

DNA, (ca. 30 MDa)

monoclonal IgG (mAb, ca.150 kDa)

hyaluronic acid (ca. 1 MDa)

polyvinylpyrrolidone (ca.50 kDa)

polyethylene glycol (ca.20 kDa)

DNA, (ca. 30 MDa)

monoclonal IgG (mAb, ca.150 kDa)

hyaluronic acid (ca. 1 MDa)

polyvinylpyrrolidone (ca.50 kDa)

Non-Porous 2 µm ODS-Silica

Reversed-phase mode for polymers up to 30 MDaSeparates almost any polymer including bio-polymers and synthetic polymers

Amazing number of peaks for peptides and proteinsDifferent selectivity from porous ODS columns

250mm length high-resolution column with 2um particleHigh-efficiency with low flow rate compatible with conventional HPLC systems

Non-Porous 2 µm ODS-Silica

Imtakt Corp./JAPAN ( www.imtakt.com )Imtakt USA ( www.imtaktUSA.com )North America Other Countries

Ordering Information

Amazing separations for many molecular weights from peptides to proteins

2µm Non-Porous Silica, Octadecyl Ligand, End-Capped

Micro / Nano columns are also available.

Product CodeLength(mm)1020305075

100150250

FF020FF029FF021FF022FF023FF024FF025FF026

2 mm I.D.FF030FF039FF031FF032FF033FF034FF035FF036

3 mm I.D.--

FF011FF012FF013FF014FF015FF016

1 mm I.D.FF000FF009FF001FF002FF003FF004FF005FF006

4.6 mm I.D.--

FF061FF062FF063FF064FF065FF066

6 mm I.D.--

FF0P1FF0P2FF0P3FF0P4FF0P5FF0P6

10 mm I.D.

0 50 100 150 min

A: water /TFA = 100 /0.1B: acetonitrile /TFA = 100 /0.150deg.C, 220nmTryptic digest of alpha-casein5uL

Presto FF-C18 (2um)250 x 4.6 mm1-35%B (0-150min)0.4mL/min (25MPa)

Porous ODS (3um)250 x 4.6 mm5-45%B (0-150min)1mL/min (14MPa)

268 peaks

131 peaks

Peptide Mapping

Presto FF-C18, 50 x 4.6 mmA: water /TFA = 100 /0.1B: acetonitrile /TFA = 100 /0.0710-60%B (0-20min)0.3mL/min (5MPa), 37deg.CELSD0.6-3uL

Monoclonal IgG, Anti-hUK(H)

0 10 20 min

Polyclonal IgG, Human Serum

Monoclonal Antibody

Presto FF-C18, a non-porous ODS HPLC column, performs exceptionally well with peptide separations.HPLC and LC-MS analysis of a small amount of peptides is important for proteomics. Presto FF-C18 can achieve twice as many peaks as conventional porous ODS columns.Non-porous ODS Presto FF-C18 may change the proteomics field dramatically.

Immunoglobulin G (IgG), a large protein (MW ca. 150kDa), can exhibit poor peak shape when injected onto porous RP columns, due to high mass transfer resistance.Non-porous ODS Presto FF-C18 can provide improved peak shape and recognition between mAb (same amino acid sequence) and polyclonal antibody (different amino acid sequence).Presto FF-C18 may change the antibody separation field, which has had difficulty using traditional porous columns.

BRFF0E-JB20B