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THE CONCEPTION OF CYTOKIN-ADHESIN PATHOOENESIS OF PSORIASIS AND ITS FURTHER PERFECTION. Andris Rubins. Hackel

Weksler. Rona Hartmane, Ymis Lielbriedis, Chair of oemtatovenerology, Latvian Academy of Medicine, Rtga. latvta

An attempt has been made to perfect the conception of cytokin-adhesin pathogen&s of psoriasis advanced by the authors generalizing the results obtained from funher research into various forms of the disease (vulgaris. erytrodemtatic. anhropatic) in 112 patients.

Monoclonal antibodies (MA) have been used to T-l~mphccyte integrin- adhesin proteins, immunocyte CD3’, CD4’, CDg’, CDlla Also LFA-I (LA1 test) and modulation pa~liaities of kttmunccyte reception concerning interferon y (IFNy), interleukin 2 (IL-2) and tumor necrotic factor TNF-a (LA1 modulation test) as well as intracutaneous test with FHA and mycohacteria.

It has been proved that particularly in eryncdermatic and arthmpatic psoriasis the activity of illness correlate with adhesin CD3’. CDlla’. CD4’. CDg’, as well as reaction increase in blood T-lymphocyte cytokins - LFA- I, IFN-y and TNF-a. Sensihilization of psoriatic demtal T-lymphocytes to mywbacteria has been detemtined.

Based on these tits one may a~wme that cytokins, IFN-y and TNF-a play the decisive role in the trigger mechanism of psoriasis pathogenesis inducing the fomtation of corresponding cytokin and adhesin receptors of T-lymphocytes. Taking into consideration the fact that we and other authors have detected sensihiliration of psoriatic dental T-lymphocytes to myco- bacteria, it is possible that one of the inductor’s of psoriasis autoimmunity is heat shock (stress) protein possessing pmpenies of mimicry antigen cross-like to mycobacterial and keratonocyte antigens of the patients’ tissues.

343 QUANTITATIVE ANALYSIS OF IL-8 (INTERLEUKIN 8) “RNA EXPRESSION IN PSORIASIS BY REVERSED TRANSCRIPTASE- POLYMERASE CHAIN REACTION (RT-PCR). Hidekazu

‘. Izulanl.TaluoDTomoaklpTadashirtment of Dermatology, *Department of Surgery. Kinki University School of Medicine, Osaka, Japan.

In psoriatic involved skin, neutrophilcs are infiltraled in the epidermis. The mechanism of this phenomenon is explained by the fact that keratinocytes produce and secrete the rhemotactic factor and express their adhesion molecules on the cell membranes. The aim af this study

was to determine the presence of IL-S, IL-6 and IL-la, “RNA in involved skin from psoriasis patients using the qualitative PCR, and to develop and use a quantitative PCR to determine the amount of IL-8 “RNA in involved skin. To measure the amount of IL-8 in the involved and un-involved skins of psoriasis quantitatively, IL-8 mRNA was measured using P competitive PCR method with a synthetic IL-8 RNA as an external standard that differs in size from native RNA. The percentage

of psoriasis specimens expressing IL-S, IL-6 and IL-la “RNA was increased in the involved skin in comparison with non-lesional skin of psoriasis patients under normal control. In addition, using the quantitative PCR method, there were significantly higher levels of IL-8 “RNA in the involved skin, in comparison with the one in the non- involved skins. These data suggest that IL-8 may have an important role in the augmentation of the inflammalory process in psoriatic Iesional skin.

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