Kam W. Leong, Ph.D. James B. Duke Professor Department of

Curriculum vitae of Kam W. Leong, PhD

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Kam W. Leong, Ph.D.

James B. Duke Professor Department of Biomedical Engineering

Pratt School of Engineering Duke University

1395 CIEMAS, BOX 90281, Durham, NC 27708 Phone (919) 660-‐8466; Fax (919) 684-‐4488; e-‐mail:[email protected]

Professional Experience:

1986-‐1991 Johns Hopkins University (JHU)

Assistant Professor, Department of Biomedical Engineering, School of Medicine; and joint appointment in Department of Mater Sci Eng, School of Engineering

1991-‐1998 JHU

Associate Professor, Department of Biomedical Engineering, School of Medicine; and joint appointment in Department of Mater Sci Eng

1998-‐2005 JHU

Professor, Department of Biomedical Engineering, School of Medicine; and joint appointment in Department of Orthopedic Surgery

JHU Director of Master Program in Biomedical Engineering, 1990-‐2005 Program Director and Technical Advisor to Institute of Materials Research and Engineering (IMRE), Singapore, 1998-‐2004 Principal Investigator, Division of Johns Hopkins in Singapore, 1999-‐2006

2006-‐2009 2006-‐Present Duke University

Principal Investigator, Stem Cell Bioengineering Laboratory Duke-‐NUS Graduate Medical School, Singapore Director of Bioengineering Initiative on Nanomedicine/Nanotherapeutics, Duke University Professor, Department of Biomedical Engineering, School of Engineering; and joint appointment in Department of Surgery, School of Medicine, and Department of Mechanical Engineering and Materials Science

Education: University of California, Santa Barbara, CA B.S. in Chemical Engineering, 1973-‐1977 University of Pennsylvania, Philadelphia, PA Ph.D. in Chemical Engineering, 1977-‐1982, degree conferred 1987 Massachusetts Institute of Technology, Cambridge, MA Research Associate in Whitaker College of Health Sciences and Technology and Department

of Applied Biological Sciences, 1982-‐1985 Honor: Johnson-‐Johnson Postdoctoral Fellowship for Biomedical Research, 1983-‐1985, MIT Young Investigator Research Achievement Award of Controlled Release Society, 1994 Honorary Professor, Wuhan University, China, 1994-‐present

The Chinese-‐American Chemical Society Award for Recognition of Pioneering Contributions in Polymer Chemistry and Biomedical Engineering, 1995

Excellence in Guidance of Graduate Student Research, Controlled Release Society 1993: CRS-‐Proctor & Gamble Recognition Award 1996: CRS-‐3M Pharmaceuticals Recognition Award 1997: CRS-‐Cygnus Recognition Award


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Capsugel Award on Innovative Aspects of Controlled Release Research, Controlled Release Society: 1996, 1997, 1998, 2000, 2001

Fellow of American Institute for Medical and Biological Engineering, 1998 Student Award (Hunter Chen) for Excellence in Research, American Soc. for Gene Therapy,

2007 Best Manuscript Award of Acta Biomaterialia, 2006 J Controlled Release Jorge Heller Best Manuscript Award, 2007

Stansell Family Distinguished Research Award, 2010 Distinguished Visiting Professor of National University of Singapore, 2006-‐present Society for Biomaterials Clemson Award for Applied Research, 2012 Elected into National Academy of Engineering, 2013

Teaching Activity:

Cell and Tissue Engineering; Biomedical Polymers; Advanced Biomaterials, Introduction to Biomaterials; Nanomedicine

Research Projects:

Biomaterials Design: • design of self-‐assembled fibers controlled release electrospun fibers for tissue engineering • synthesis of new biodegradable polymers and new polyelectrolytes for drug and gene delivery

• design of nucleic acid-‐binding polymers as new anti-‐inflammatory agents • design of microfluidic platforms related to nanomedicine Controlled Drug and Gene Delivery: • oral gene delivery for hemophilia A • generation of transdifferentiated cells by nonviral vectors • oral delivery of antigen genes for vaccination Tissue Engineering: • study of interaction of stem cells with biofunctional polymeric surface • study of interaction of embryonic and adult stem cells with synthetic nanostructured biomaterials

• functional tissue engineering with induced human pluripotent stem cells and transdifferentiated cells

• development of bioartificial nucleus pulposus device

Professional Activity: NIH Review Panels NIDA and NIH-‐SBIR Ad Hoc Research Contract Review Committees, 1986, 1990-‐92,

1995, 1996, 1997 NIH Ad Hoc Study Sections, NIGMS, 1995-‐2004, 2005, 2006, 2007, 2008 NIH Gene and Drug Delivery Study Section, NIBIB, 2004-‐2005, 2007-‐2011

NSF Review Panels NSF Biomaterials and Bioengineering review panels, 1993-‐1999, 2002 NSF-‐Engineering Research Center Site Visit panels, 1995, 1999

Other Review and Advisory Panels

American Cancer Society Institutional Research Grant Committee, 1988 Review Panel of National Health Research Institute, Taiwan, 2002 -‐ present Nanoscience and Nanotechnology Research Grant Committee, Singapore, 2002 Advisory Panel of Nanoscience and Nanotechnology Initiative, Singapore, 2002 Advisory Panel of Div. of Bioengineering at National University of Singapore, 2003 Reviewer for Research Grants Council of Hong Kong, 2004-‐2006 Advisory Panel of Tissue Engineering Program at National University of Singapore,

2005, 2007 International Advisory Panel for Chongqing University, China, 2012


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Symposia Organized Chairman of Symposium on Polymeric Controlled Release

ACS National Meeting, Dallas, April, 1989 Co-‐chairman on Session of Materials for Drug Delivery AICHE Summer Meeting, Philadelphia, August, 1989 Amer. Cancer Society Institutional Research Grant Committee, 1988 Chairman of Symposium on Polymeric Controlled Release ACS National Meeting, Dallas, April, 1989 Co-‐chairman on Session of Materials for Drug Delivery Co-‐chairman of Symposium on Polymer-‐Cell Interactions AICHE Annual Meeting, Miami, November, 1992 Co-‐chairman of Symposium on Polymers in Medicine and Pharmacy MRS Annual Meeting, San Francisco, 1995 Chairman of Tissue Engineering and Stem Cell Technology New York Academy of Sciences, New York, 2002 Co-‐chairman of Workshop on Stem Cell Tissue Engineering Biomedical Engineering Society Meeting, Baltimore, 2005 Co-‐chairman on Session of Gene Therapy for Tissue Engineering Biomedical Engineering Society Meeting, Baltimore, 2005 Program co-‐chairman of Controlled Release Society Meeting, New York 2008 Organizer for Session on Derivatization of Transdifferentiated Cells Society for Biomaterials Meeting, New Orleans, October, 2012

Service Within University (JHU)

Graduate Committee of Whiting School of Engineering, 1999 -‐ 2005 Chairman of ad hoc committees on faculty promotion, School of Engineering, 2002,

2004, 2005 Steering Committee for Hopkins Institute of Bionanotechnology, 2004 – 2006 Faculty ad hoc promotion committees, 2006-‐present Faculty search committees, 2006-‐present Dean search committee, 2007-‐2008 Provost Lectureship committee, 2007-‐2008

Duke University

Other Activities Honorary Professor, Wuhan University, China

Member of NSF Delegation of US-‐South Korea Collaboration in Biomedical Engineering, Seoul, South Korea, 1997

Participant of Interagency Working Group on Nanoscience, Engineering, and Technology, Sponsored by National Science and Technology Council, 1999

Member of NSF Delegation of US-‐Japan Joint Symposium on Nanomedicine, Yokohama, Japan, 2002

Presenter at NCI Workshop on Building the Interface of Nanotechnology and Cancer Imaging Research, 2004

Member of NSF Delegation for US-‐Mauritius Collaboration in Biomaterials, Workshop: Perspectives and Opportunities, Mauritius, 2009

Journal Review Reviewer for Journals: Nature; Nature Medicine; Nature Materials; Nature

Biotechnology; Science; J. Polym. Sci.; Biomaterials; Pharm. Research; J. Controlled Release; Polymer; J. AICHE; J. Pharm. Sci.; J. Biomat. Sci.; Biotech. Bioeng.; Chem. Eng. Sci.; Macromolecules; Biomacromolecules; Advanced Materials; J. Biomed. Mat. Res.; ASAIO; JACS; Mol Ther; J. GeneMedicine; Gene Therapy; Int. J. Nanomedicine


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Professional Membership:

Biomedical Engineering Society; Controlled Release Society; Society of Biomaterials; Am. Inst. Medical and Biological Engineering; Am. Soc. Gene Therapy

Editorial Board • Molecular Therapy • Biomaterials • Acta Biomaterialia • J Biomat. Sci., Poly Ed. • J

Controlled Release • Genetic Vaccines and Therapy • Int. J Nanomedicine • Nanomedicine • Biomacromolecules • Nano Today

Publications: ~260 refereed journal manuscripts; ~30 reviews and book chapters; ~400 Conference

proceeding and abstracts; ~40 issued patents, ~30 published patent applications (Total number of citations via Google Scholar ~ 17,100; h-‐index = 67)

Refereed Journal Papers 1. Leong K, Forsman W. Vapor-‐phase intercalation by alcl3-‐hcl mixtures. Carbon, 20(2): p.132-‐132. (1982) 2. Leong K, Forsman WC, Vogel FL. Intercalation of graphite with the adducts of nitrosyl chloride and metal

chlorides. Carbon, 20(2): p.135-‐135. (1982) 3. Forsman WC, Dziemianowicz T, Leong K, Carl D. Graphite-‐intercalation chemistry -‐ an interpretive review.

Synthetic Metals, 5(2): p.77-‐100. (1983) 4. Leong K, Forsman WC. Electron-‐transfer to protons in graphite-‐intercalation. Synthetic Metals, 6(1): p.61-‐

63. (1983) 5. Leong K, Tome A, Dziemianowicz T, Forsman W. Intercalation of transition-‐metal dichlorides with no as

coreagent. Synthetic Metals, 7(1-‐2): p.141-‐141. (1983) 6. Leong K, Forsman WC, Vogel FL. Conversion of carbon graphite fibers to fibers of graphite oxide. Materials

Science and Engineering, 64(2): p.149-‐155. (1984) 7. Langer R, Siegel R, Brown L, Leong K, Kost J, Edelman E. Controlled release and magnetically modulated

systems for macromolecular drugs. Annals of the New York Academy of Sciences, 446: p.1-‐13. (1985) 8. Leong KW, Brott BC, Langer R. Bioerodible polyanhydrides as drug-‐carrier matrices .1. Characterization,

degradation, and release characteristics. Journal of Biomedical Materials Research, 19(8): p.941-‐955. (1985) 9. Leong KW, Kost J, Mathiowitz E, Langer R. Polyanhydrides for controlled release of bioactive agents.

Biomaterials, 7(5): p.364-‐371. (1986) 10. Langer R, Siegel R, Brown L, Leong K, Kost J, Edelman E. Controlled release -‐ 3 mechanisms. Chemtech, 16(2):

p.108-‐110. (1986) 11. Leong KW, Damore P, Marletta M, Langer R. Bioerodible polyanhydrides as drug-‐carrier matrices .2.

Biocompatibility and chemical-‐reactivity. Journal of Biomedical Materials Research, 20(1): p.51-‐64. (1986) 12. Leong KW, Simonte V, Langer R. Synthesis of polyanhydrides -‐ melt-‐polycondensation, dehydrochlorination,

and dehydrative coupling. Macromolecules, 20(4): p.705-‐712. (1987) 13. Lee DA, Flores RA, Anderson PJ, Leong KW, Teekhasaenee C, Dekater AW, Hertzmark E. Glaucoma filtration

surgery in rabbits using bioerodible polymers and 5-‐fluorouracil. Ophthalmology, 94(12): p.1523-‐1530. (1987)

14. Lee DA, Leong KW, Panek WC, Eng CT, Glasgow BJ. The use of bioerodible polymers and 5-‐fluorouracil in glaucoma filtration surgery. Investigative Ophthalmology & Visual Science, 29(11): p.1692-‐1697. (1988)

15. Bindschaedler C, Leong K, Mathiowitz E, Langer R. Polyanhydride microsphere formulation by solvent-‐extraction. Journal of Pharmaceutical Sciences, 77(8): p.696-‐698. (1988)

16. Kost J, Leong K, Langer R. Ultrasonically controlled polymeric drug delivery. Makromolekulare Chemie-‐Macromolecular Symposia, 19: p.275-‐285. (1988)

17. Kost J, Leong K, Langer R. Ultrasound-‐enhanced polymer degradation and release of incorporated substances -‐ (controlled release drug delivery systems). Proceedings of the National Academy of Sciences of the United States of America, 86(20): p.7663-‐7666. (1989)


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18. Brem H, Kader A, Epstein JI, Tamargo RJ, Domb A, Langer R, Leong KW. Biocompatibility of a biodegradable, controlled-‐release polymer in the rabbit brain. Selective Cancer Therapeutics, 5(2): p.55-‐65. (1989)

19. Jampel HD, Leong KW, Dunkelburger GR, Quigley HA. Glaucoma filtration surgery in monkeys using 5-‐fluorouridine in polyanhydride disks. Archives of Ophthalmology, 108(3): p.430-‐435. (1990)

20. Tamargo RJ, Leong KW, Brem H. Growth-‐inhibition of the 9l glioma using polymers to release heparin and cortisone-‐acetate. Journal of Neuro-‐Oncology, 9(2): p.131-‐138. (1990)

21. Shi FY, Wang LF, Tashev E, Leong KW. Synthesis and characterization of hydrolytically labile poly(phosphoester urethanes). ACS Symposium Series, 469: p.141-‐154. (1991)

22. Koya P, Jampel H, Quigley H, Leong K. Pharmacokinetics of vp-‐16 release from polyanhydrides after experimental filtration surgery. Investigative Ophthalmology & Visual Science, 32(4): p.745-‐745. (1991)

23. Richards M, Dahiyat BI, Arm DM, Brown PR, Leong KW. Evaluation of polyphosphates and polyphosphonates as degradable biomaterials. Journal of Biomedical Materials Research, 25(9): p.1151-‐1167. (1991)

24. Saltzman WM, Parsonswingerter P, Leong KW, Lin S. Fibroblast and hepatocyte behavior on synthetic-‐polymer surfaces. Journal of Biomedical Materials Research, 25(6): p.741-‐759. (1991)

25. Richards M, Dahiyat BI, Arm DM, Lin S, Leong KW. Interfacial polycondensation and characterization of polyphosphates and polyphosphonates. Journal of Polymer Science Part a-‐Polymer Chemistry, 29(8): p.1157-‐1165. (1991)

26. Jampel HD, Koya P, Leong K, Quigley HA. Invitro release of hydrophobic drugs from polyanhydride disks. Ophthalmic Surgery and Lasers, 22(11): p.676-‐680. (1991)

27. Charles JB, Ganthier R, Wilson MR, Lee DA, Baker RS, Leong KW, Glasgow BJ. Use of bioerodible polymers impregnated with mitomycin in glaucoma filtration surgery in rabbits. Ophthalmology, 98(4): p.503-‐508. (1991)

28. Heffez DS, Leong KW. Sustained-‐release of papaverine for the treatment of cerebral vasospasm -‐ invitro evaluation of release kinetics and biological-‐activity. Journal of Neurosurgery, 77(5): p.783-‐787. (1992)

29. Brown K, Leong K, Bathon L. A novel controlled-‐release intraarticular delivery system. Arthritis and Rheumatism, 36(9): p.S267-‐S267. (1993)

30. Golumbek PT, Azhari R, Jaffee EM, Levitsky HI, Lazenby A, Leong K, Pardoll DM. Controlled-‐release, biodegradable cytokine depots -‐ a new approach in cancer vaccine design. Cancer Research, 53(24): p.5841-‐5844. (1993)

31. Dahiyat BI, Hostin E, Posadas EM, Leong KW. Synthesis and characterization of putrescine-‐based poly(phosphoester-‐urethanes). Journal of Biomaterials Science-‐Polymer Edition, 4(5): p.529-‐543. (1993)

32. Reisfeld B, Blackband S, Calhoun V, Grossman S, Eller S, Leong K. The use of magnetic-‐resonance-‐imaging to track controlled drug release and transport in the brain. Magnetic Resonance Imaging, 11(2): p.247-‐252. (1993)

33. Lin ST, Krebs SL, Kadiyala S, Leong KW, Lacourse WC, Kumar B. Development of bioabsorbable glass-‐fibers. Biomaterials, 15(13): p.1057-‐1061. (1994)

34. Gutsche AT, Parsonswingerter P, Chand D, Saltzman WM, Leong KW. N-‐acetylglucosamine and adenosine derivatized surfaces for cell-‐culture -‐ 3t3 fibroblast and chicken hepatocyte response. Biotechnology and Bioengineering, 43(8): p.801-‐809. (1994)

35. Jenkins GM, Leong K, Heller P, Sollott S, Kinsella J, Nater C, Oconnor F, Froehlich J, Cheng L. Local-‐delivery of taxol inhibits neointimal regrowth following balloon injury of the rat carotid-‐artery. Circulation, 90(4): p.297-‐297. (1994)

36. Uppal P, Jampel HD, Quigley HA, Leong KW. Pharmacokinetics of etoposide delivery by a bioerodible drug carrier implanted at glaucoma surgery. Journal of Ocular Pharmacology, 10(2): p.471-‐479. (1994)

37. Leong KW, Mao HQ, Zhuo RX. Biodegradable polymers with a phosphoryl-‐containing backbone: Tissue engineering and controlled drug delivery applications. Chinese Journal of Polymer Science, 13(4): p.289-‐314. (1995)

38. Shao W, Leong KW. Microcapsules obtained from complex coacervation of collagen and chondroitin sulfate. Journal of Biomaterials Science-‐Polymer Edition, 7(5): p.389-‐399. (1995)


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39. Zhao Z, Jaffee E, Pardoll D, Leong K. Cytokine delivery by biodegradable beads as alternative to gene-‐transduced tumors for augmenting antitumor immune-‐responses. Journal of Cellular Biochemistry: p.183-‐183. (1995)

40. Dahiyat BI, Richards M, Leong KW. Controlled-‐release from poly(phosphoester) matrices. Journal of Controlled Release, 33(1): p.13-‐21. (1995)

41. Reisfeld B, Kalyanasundaram S, Leong K. A mathematical-‐model of polymeric controlled drug-‐release and transport in the brain. Journal of Controlled Release, 36(3): p.199-‐207. (1995)

42. Dahiyat BI, Posadas EM, Hirosue S, Hostin E, Leong KW. Degradable biomaterials with elastomeric characteristics and drug-‐carrier function. Reactive Polymers, 25(2-‐3): p.101-‐109. (1995)

43. Gutsche A, Lo H, Zurlo J, Yager J, Leong K. Engineering of a sugar-‐derivatized porous network for hepatocyte culture. Biomaterials, 17(3): p.387-‐393. (1996)

44. Hollinger J, Leong K. Poly(alpha-‐hydroxy acids): Carriers for bone morphogenetic proteins. Biomaterials, 17(2): p.187-‐194. (1996)

45. Gutsche A, Zurlo J, Deyesu E, Leong K. Rat hepatocyte morphology and function on lactose-‐derivatized polystyrene surfaces. Biotechnology and Bioengineering, 49(3): p.259-‐265. (1996)

46. Lo H, Kadiyala S, Guggino S, Leong K. Poly(l-‐lactic acid) foams with cell seeding and controlled-‐release capacity. Journal of Biomedical Materials Research, 30(4): p.475-‐484. (1996)

47. Zhao Z, Leong K. Controlled delivery of antigens and adjuvants in vaccine development. Journal of Pharmaceutical Sciences, 85(12): p.1261-‐1270. (1996)

48. Lesser G, Grossman S, Leong K, Lo H, Eller S. In vitro and in vivo studies of subcutaneous hydromorphone implants designed for the treatment of cancer pain. Pain, 65(2-‐3): p.265-‐272. (1996)

49. Kalyanasundaram S, Calhoun V, Leong K. A finite element model for predicting the distribution of drugs delivered intracranially to the brain. American Journal of Physiology-‐Regulatory Integrative and Comparative Physiology, 273(5): p.R1810-‐R1821. (1997)

50. Hu W, Wang L, Leong K. Synthesis and characterization of methacrylic derivatives as drug carriers. Drug Development and Industrial Pharmacy, 23(7): p.671-‐678. (1997)

51. Kalyanasundaram S, Leong K. Intracranial drug delivery systems. Stp Pharma Sciences, 7(1): p.62-‐70. (1997) 52. Brown K, Leong K, Huang C, Dalal R, Green G, Haimes H, Jimenez P, Bathon J. Gelatin/chondroitin 6-‐sulfate

microspheres for the delivery of therapeutic proteins to the joint. Arthritis and Rheumatism, 41(12): p.2185-‐2195. (1998)

53. Truong-‐Le V, August J, Leong K. Controlled gene delivery by DNA-‐gelatin nanospheres. Human Gene Therapy, 9(12): p.1709-‐1717. (1998)

54. Leong K, Mao H, Truong-‐Le V, Roy K, Walsh S, August J. DNA-‐polycation nanospheres as non-‐viral gene delivery vehicles. Journal of Controlled Release, 53(1-‐3): p.183-‐193. (1998)

55. Truong-‐Le V, Walsh S, Schweibert E, Mao H, Guggino W, August J, Leong K. Gene transfer by DNA-‐gelatin nanospheres. Archives of Biochemistry and Biophysics, 361(1): p.47-‐56. (1999)

56. Kalyanasundaram S, Feinstein S, Nicholson J, Leong K, Garver R. Coacervate microspheres as carriers of recombinant adenoviruses. Cancer Gene Therapy, 6(2): p.107-‐112. (1999)

57. Roy K, Mao H, Huang S, Leong K. Oral gene delivery with chitosan-‐DNA nanoparticles generates immunologic protection in a murine model of peanut allergy. Nature Medicine, 5(4): p.387-‐391. (1999)

58. Georgantas R, Leong K, August J. Antigen-‐specific induction of peripheral t cell tolerance in vivo by codelivery of DNA vectors encoding antigen and fas ligand. Human Gene Therapy, 11(6): p.851-‐858. (2000)

59. Chia S, Leong K, Li J, Xu X, Zeng K, Er P, Gao S, Yu H. Hepatocyte encapsulation for enhanced cellular functions. Tissue Engineering, 6(5): p.481-‐495. (2000)

60. Fang N, Chan V, Mao H, Leong K. Interactions of phospholipid bilayer with chitosan: Effect of molecular weight and ph. Biomacromolecules, 2(4): p.1161-‐1168. (2001)

61. Wan A, Mao H, Wang S, Leong K, Ong L, Yu H. Fabrication of poly(phosphoester) nerve guides by immersion precipitation and the control of porosity. Biomaterials, 22(10): p.1147-‐1156. (2001)


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62. Wang S, Wan A, Xu X, Gao S, Mao H, Leong K, Yu H. A new nerve guide conduit material composed of a biodegradable poly(phosphoester). Biomaterials, 22(10): p.1157-‐1169. (2001)

63. Ramakrishna S, Mayer J, Wintermantel E, Leong K. Biomedical applications of polymer-‐composite materials: A review. Composites Science and Technology, 61(9): p.1189-‐1224. (2001)

64. Mao H, Roy K, Troung-‐Le V, Janes K, Lin K, Wang Y, August J, Leong K. Chitosan-‐DNA nanoparticles as gene carriers: Synthesis, characterization and transfection efficiency. Journal of Controlled Release, 70(3): p.399-‐421. (2001)

65. Wang J, Mao H, Leong K. A novel biodegradable gene carrier based on polyphosphoester. Journal of the American Chemical Society, 123(38): p.9480-‐9481. (2001)

66. Chan V, Mao H, Leong K. Chitosan-‐induced perturbation of dipalmitoyl-‐sn-‐glycero-‐3-‐phosphocholine membrane bilayer. Langmuir, 17(12): p.3749-‐3756. (2001)

67. Li J, Li X, Toh K, Ni X, Zhou Z, Leong K. Inclusion complexation and formation of polypseudorotaxanes between poly[(ethylene oxide)-‐ran-‐(propylene oxide)] and cyclodextrins. Macromolecules, 34(26): p.8829-‐8831. (2001)

68. Li J, Li X, Zhou Z, Ni X, Leong K. Formation of supramolecular hydrogels induced by inclusion complexation between pluronics and alpha-‐cyclodextrin. Macromolecules, 34(21): p.7236-‐7237. (2001)

69. Wang S, Ma N, Gao S, Yu H, Leong K. Transgene expression in the brain stem effected by intramuscular injection of polyethylenimine/DNA complexes. Molecular Therapy, 3(5): p.658-‐664. (2001)

70. Hanes J, Sills A, Zhao Z, Suh K, Tyler B, DiMeco F, Brat D, Choti M, Leong K, Pardoll D, Brem H. Controlled local delivery of interleukin-‐2 by biodegradable polymers protects animals from experimental brain tumors and liver tumors. Pharmaceutical Research, 18(7): p.899-‐906. (2001)

71. Chew J, Fu T, Mao H, Leong K, Chua K. Oral administration of major house dust mite allergen genes complexed with chitosan elicits protective th1-‐skewed immunity in mice. Allergy, 57: p.64-‐64. (2002)

72. Chia S, Wan A, Quek C, Mao H, Xu X, Shen L, Ng M, Leong K, Yu H. Multi-‐layered microcapsules for cell encapsulation. Biomaterials, 23(3): p.849-‐856. (2002)

73. Xu X, Yu H, Gao S, Mao H, Leong K, Wang S. Polyphosphoester microspheres for sustained release of biologically active nerve growth factor. Biomaterials, 23(17): p.3765-‐3772. (2002)

74. Du X, Yang Y, Le Visage C, Chen H, DeJong R, Wang D, Leong K, Hamper U, Yang X. In vivo ultrasound imaging of catheter-‐based vascular gene/microsphere delivery. Circulation, 106(19): p.M-‐M. (2002)

75. Fang N, Chan V, Wan K, Mao H, Leong K. Colloidal adhesion of phospholipid vesicles: High-‐resolution reflection interference contrast microscopy and theory. Colloids and Surfaces B-‐Biointerfaces, 25(4): p.347-‐362. (2002)

76. Wang J, Zhang P, Mao H, Leong K. Enhanced gene expression in mouse muscle by sustained release of plasmid DNA using ppe-‐ea as a carrier. Gene Therapy, 9(18): p.1254-‐1261. (2002)

77. Kumar M, Behera A, Lockey R, Zhang J, Bhullar G, De La Cruz C, Chen L, Leong K, Huang S, Mohapatra S. Intranasal gene transfer by chitosan-‐DNA nanospheres protects balb/c mice against acute respiratory syncytial virus infection. Human Gene Therapy, 13(12): p.1415-‐1425. (2002)

78. Peng B, Liu S, Kuang M, He Q, Totsuka S, Huang L, Huang J, Lu M, Liang L, Leong K, Ohno T. Autologous fixed tumor vaccine: A formulation with cytokine-‐microparticles for protective immunity against recurrence of human hepatocellular carcinoma. Japanese Journal of Cancer Research, 93(4): p.363-‐368. (2002)

79. Wang J, Zhang P, Lu H, Ma N, Wang S, Mao H, Leong K. New polyphosphoramidate with a spermidine side chain as a gene carrier. Journal of Controlled Release, 83(1): p.157-‐168. (2002)

80. Zhao Z, Wang J, Mao H, Leong K. Polyphosphoesters in drug and gene delivery. Advanced Drug Delivery Reviews, 55(4): p.483-‐499. (2003)

81. Li J, Ni X, Leong K. Block-‐selected molecular recognition and formation of polypseudorotaxanes between poly(propylene oxide)-‐poly (ethylene oxide)poly(propylene oxide) triblock copolymers and alpha-‐cyclodextrin. Angewandte Chemie-‐International Edition, 42(1): p.69-‐72. (2003)


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82. Ying L, Yin C, Zhuo R, Leong K, Mao H, Kang E, Neoh K. Immobilization of galactose ligands on acrylic acid graft-‐copolymerized poly(ethylene terephthalate) film and its application to hepatocyte culture. Biomacromolecules, 4(1): p.157-‐165. (2003)

83. Lu H, Lim W, Wang J, Tang Z, Zhang P, Leong K, Chia S, Yu H, Mao H. Galactosylated pvdf membrane promotes hepatocyte attachment and functional maintenance. Biomaterials, 24(27): p.4893-‐4903. (2003)

84. Xu X, Yee W, Hwang P, Yu H, Wan A, Gao S, Boon K, Mao H, Leong K, Wang S. Peripheral nerve regeneration with sustained release of poly(phosphoester) microencapsulated nerve growth factor within nerve guide conduits. Biomaterials, 24(13): p.2405-‐2412. (2003)

85. Yin C, Chia S, Quek C, Yu H, Zhuo R, Leong K, Mao H. Microcapsules with improved mechanical stability for hepatocyte culture. Biomaterials, 24(10): p.1771-‐1780. (2003)

86. Yin C, Liao K, Mao H, Leong K, Zhuo R, Chan V. Adhesion contact dynamics of hepg2 cells on galactose-‐immobilized substrates. Biomaterials, 24(5): p.837-‐850. (2003)

87. Fang N, Wang J, Mao H, Leong K, Chan V. Bhem-‐chol/dope liposome induced perturbation of phospholipid bilayer. Colloids and Surfaces B-‐Biointerfaces, 29(4): p.233-‐245. (2003)

88. Shi L, Tang G, Gao S, Ma Y, Liu B, Li Y, Zeng J, Ng Y, Leong K, Wang S. Repeated intrathecal administration of plasmid DNA complexed with polyethylene glycol-‐grafted polyethylenimine led to prolonged transgene expression in the spinal cord. Gene Therapy, 10(14): p.1179-‐1188. (2003)

89. Wang J, Huang S, Zhang P, Mao H, Leong K. Effect of side-‐chain structures on gene transfer efficiency of biodegradable cationic polyphosphoesters. International Journal of Pharmaceutics, 265(1-‐2): p.75-‐84. (2003)

90. Li J, Ni X, Leong K. Injectable drug-‐delivery systems based on supramolecular hydrogels formed by poly(ethylene oxide) and alpha-‐cyclodextrin. Journal of Biomedical Materials Research Part a, 65A(2): p.196-‐202. (2003)

91. Yin C, Ying L, Zhang P, Zhuo R, Kang E, Leong K, Mao H. High density of immobilized galactose ligand enhances hepatocyte attachment and function. Journal of Biomedical Materials Research Part a, 67A(4): p.1093-‐1104. (2003)

92. Liu X, Yang Y, Leong K. Thermally responsive polymeric micellar nanoparticles self-‐assembled from cholesteryl end-‐capped random poly(n-‐isopropylacrylamide-‐co-‐n,n-‐dimethylacrylamide): Synthesis, temperature-‐sensitivity, and morphologies. Journal of Colloid and Interface Science, 266(2): p.295-‐303. (2003)

93. Wen J, Kim G, Leong K. Poly(d,llactide-‐co-‐ethyl ethylene phosphate)s as new drug carriers. Journal of Controlled Release, 92(1-‐2): p.39-‐48. (2003)

94. Li J, Ni X, Zhou Z, Leong K. Preparation and characterization of polypseudorotaxanes based on block-‐selected inclusion complexation between poly(propylene oxide)-‐poly(ethylene oxide)-‐poly(propylene oxide) triblock copolymers and alpha-‐cyclodextrin. Journal of the American Chemical Society, 125(7): p.1788-‐1795. (2003)

95. Li J, Li X, Ni X, Leong K. Synthesis and characterization of new biodegradable amphiphilic poly(ethylene oxide)-‐b-‐poly[(r)-‐3-‐hydroxy butyratel-‐b-‐poly(ethylene oxide) triblock copolymers. Macromolecules, 36(8): p.2661-‐2667. (2003)

96. Li X, Li J, Leong K. Preparation and characterization of inclusion complexes of biodegradable amphiphilic poly(ethylene oxide)-‐poly[(r)-‐3-‐hydroxybutyrate]-‐poly(ethylene oxide) triblock copolymers with cyclodextrins. Macromolecules, 36(4): p.1209-‐1214. (2003)

97. Salem A, Searson P, Leong K. Multifunctional nanorods for gene delivery. Nature Materials, 2(10): p.668-‐671. (2003)

98. Du X, Yang Y, Le Visage C, Chen H, DeJong R, Qiu B, Wang D, Leong K, Hamper U, Yang X. In vivo us monitoring of catheter-‐based vascular delivery of gene microspheres in pigs: Feasibility. Radiology, 228(2): p.555-‐559. (2003)

99. Sun T, Chan M, Zhou Y, Xu X, Zhang J, Lao X, Wang X, Quek C, Chen J, Leong K, Yu H. Use of ultrathin shell microcapsules of hepatocytes in bioartificial liver-‐assist device. Tissue Engineering, 9: p.S65-‐S75. (2003)


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100. Chew J, Wolfowicz C, Mao H, Leong K, Chua K. Chitosan nanoparticles containing plasmid DNA encoding house dust mite allergen, der p 1 for oral vaccination in mice. Vaccine, 21(21-‐22): p.2720-‐2729. (2003)

101. Salem A, Chao J, Leong K, Searson P. Receptor-‐mediated self-‐assembly of multi-‐component magnetic nanowires. Advanced Materials, 16(3): p.268-‐+. (2004)

102. Sun D, Leong K. A nonlinear hyperelastic mixture theory model for anisotropy, transport, and swelling of annulus fibrosus. Annals of Biomedical Engineering, 32(1): p.92-‐102. (2004)

103. Huang S, Wang J, Zhang P, Mao H, Zhuo R, Leong K. Water-‐soluble and nonionic polyphosphoester: Synthesis, degradation, biocompatibility and enhancement of gene expression in mouse muscle. Biomacromolecules, 5(2): p.306-‐311. (2004)

104. Chan V, Liu K, Le Visage C, Ju B, Leong K. Bioadhesive characterization of poly(methylidene malonate 2.12) microparticle on model extracellular matrix. Biomaterials, 25(18): p.4327-‐4332. (2004)

105. Kiang T, Wen H, Lim H, Leong K. The effect of the degree of chitosan deacetylation on the efficiency of gene transfection. Biomaterials, 25(22): p.5293-‐5301. (2004)

106. Quek C, Li J, Sun T, Ling M, Chan H, Mao H, Gan L, Leong K, Yu H. Photo-‐crosslinkable microcapsules formed by polyelectrolyte copolymer and modified collagen for rat hepatocyte encapsulation. Biomaterials, 25(17): p.3531-‐3540. (2004)

107. Kuang M, Peng B, Lu M, Liang L, Huang J, He Q, Hua Y, Totsuka S, Liu S, Leong K, Ohno T. Phase ii randomized trial of autologous formalin-‐fixed tumor vaccine for postsurgical recurrence of hepatocellular carcinoma. Clinical Cancer Research, 10(5): p.1574-‐1579. (2004)

108. Li Y, Wang J, Lee C, Wang C, Gao S, Tang G, Ma Y, Yu H, Mao H, Leong K, Wang S. Cns gene transfer mediated by a novel controlled release system based on DNA complexes of degradable polycation ppe-‐ea: A comparison with polyethylenimine/DNA complexes. Gene Therapy, 11(1): p.109-‐114. (2004)

109. Wang J, Gao S, Zhang P, Wang S, Mao M, Leong K. Polyphosphoramidate gene carriers: Effect of charge group on gene transfer efficiency. Gene Therapy, 11(12): p.1001-‐1010. (2004)

110. Wang J, Lee I, Lim W, Chia S, Yu H, Leong K, Mao H. Evaluation of collagen and methylated collagen as gene carriers. International Journal of Pharmaceutics, 279(1-‐2): p.115-‐126. (2004)

111. Kiang T, Bright C, Cheung C, Stayton P, Hoffman A, Leong K. Formulation of chitosan-‐DNA nanoparticles with poly(propyl acrylic acid) enhances gene expression. Journal of Biomaterials Science-‐Polymer Edition, 15(11): p.1405-‐1421. (2004)

112. Bini T, Gao S, Xu X, Wang S, Ramakrishna S, Leong K. Peripheral nerve regeneration by microbraided poly(l-‐lactide-‐co-‐glycolide) biodegradable polymer fibers. Journal of Biomedical Materials Research Part a, 68A(2): p.286-‐295. (2004)

113. Li Q, Williams C, Sun D, Wang J, Leong K, Elisseeff J. Photocrosslinkable polysaccharides based on chondroitin sulfate. Journal of Biomedical Materials Research Part a, 68A(1): p.28-‐33. (2004)

114. Wan A, Yim E, Liao I, Le Visage C, Leong K. Encapsulation of biologics in self-‐assembled fibers as biostructural units for tissue engineering. Journal of Biomedical Materials Research Part a, 71A(4): p.586-‐595. (2004)

115. Wan A, Mao H, Wang S, Phua S, Lee G, Pan J, Lu S, Wang J, Leong K. Poly(phosphoester) ionomers as tissue-‐engineering scaffolds. Journal of Biomedical Materials Research Part B-‐Applied Biomaterials, 70B(1): p.91-‐102. (2004)

116. Wen J, Mao H, Li W, Lin K, Leong K. Biodegradable polyphosphoester micelles for gene delivery. Journal of Pharmaceutical Sciences, 93(8): p.2142-‐2157. (2004)

117. Le Visage C, Rioux-‐Leclercq N, Haller M, Breton P, Malavaud B, Leong K. Efficacy of paclitaxel released from bio-‐adhesive polymer microspheres on model superficial bladder cancer. Journal of Urology, 171(3): p.1324-‐1329. (2004)

118. Shin-‐Ya Y, Tsurushima H, Tsurumi T, Kajiuchi T, Leong K. Polyelectrolyte complex films derived from polyethyleneoxide-‐maleic acid copolymer and chitosan: Preparation and characterization. Macromolecular Bioscience, 4(5): p.526-‐531. (2004)

119. Wan A, Liao I, Yim E, Leong K. Mechanism of fiber formation by interfacial polyelectrolyte complexation. Macromolecules, 37(18): p.7019-‐7025. (2004)


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120. Wang J, Sun D, Shin-‐ya Y, Leong K. Stimuli-‐responsive hydrogel based on poly(propylene phosphate). Macromolecules, 37(2): p.670-‐672. (2004)

121. Faranesh A, Nastley M, de la Cruz C, Haller M, Laquerriere P, Leong K, McVeigh E. In vitro release of vascular endothelial growth factor from gadolinium-‐doped biodegradable microspheres. Magnetic Resonance in Medicine, 51(6): p.1265-‐1271. (2004)

122. Salem A, Chen M, Hayden J, Leong K, Searson P. Directed assembly of multisegment au/pt/au nanowires. Nano Letters, 4(6): p.1163-‐1165. (2004)

123. Li X, Li J, Leong K. Role of intermolecular interaction between hydrophobic blocks in block-‐selected inclusion complexation of amphiphilic poly(ethylene oxide)-‐poly[(r)-‐3-‐hydroxybutyrate]-‐poly(ethylene oxide) triblock copolymers with cyclodextrins. Polymer, 45(20): p.6845-‐6851. (2004)

124. Le Visage C, Dunham B, Flint P, Leong K. Coculture of mesenchymal stem cells and respiratory epithelial cells to engineer a human composite respiratory mucosa. Tissue Engineering, 10(9-‐10): p.1426-‐1435. (2004)

125. Lu H, Chua K, Zhang P, Lim W, Ramakrishna S, Leong K, Mao H. Three-‐dimensional co-‐culture of rat hepatocyte spheroids and nih/3t3 fibroblasts enhances hepatocyte functional maintenance. Acta Biomaterialia, 1(4): p.399-‐410. (2005)

126. Liu Y, Wu D, Zhang W, Jiang X, He C, Chung T, Goh S, Leong K. Polyethylenimine-‐grafted multiwalled carbon nanotubes for secure noncovalent immobilization and efficient delivery of DNA. Angewandte Chemie-‐International Edition, 44(30): p.4782-‐4785. (2005)

127. Chew S, Wen J, Yim E, Leong K. Sustained release of proteins from electrospun biodegradable fibers. Biomacromolecules, 6: p.2017-‐2024. (2005)

128. Wu D, Liu Y, Jiang X, Chen L, He C, Goh S, Leong K. Evaluation of hyperbranched poly(amino ester)s of amine constitutions similar to polyethylenimine for DNA delivery. Biomacromolecules, 6(6): p.3166-‐3173. (2005)

129. Zhang P, Wang J, Leong K, Mao H. Ternary complexes comprising polyphosphoramidate gene carriers with different types of charge groups improve transfection efficiency. Biomacromolecules, 6(1): p.54-‐60. (2005)

130. Zhang X, Wang X, Huang S, Zhuo R, Liu Z, Mao H, Leong K. In vitro gene delivery using polyamidoamine dendrimers with a trimesyl core. Biomacromolecules, 6(1): p.341-‐350. (2005)

131. Chua K, Lim W, Zhang P, Lu H, Wen J, Ramakrishna S, Leong K, Mao H. Stable immobilization of rat hepatocyte spheroids on galactosylated nanofiber scaffold. Biomaterials, 26(15): p.2537-‐2547. (2005)

132. Tan W, Teo G, Liao K, Leong K, Mao H, Chan V. Adhesion contact dynamics of primary hepatocytes on poly(ethylene terephthalate) surface. Biomaterials, 26(8): p.891-‐898. (2005)

133. Yim E, Reano R, Pang S, Yee A, Chen C, Leong K. Nanopattern-‐induced changes in morphology and motility of smooth muscle cells. Biomaterials, 26(26): p.5405-‐5413. (2005)

134. Fang N, Tan W, Leong K, Mao H, Chan V. Ph responsive adhesion of phospholipid vesicle on poly(acrylic acid) cushion grafted to poly(ethylene terephthalate) surface. Colloids and Surfaces B-‐Biointerfaces, 42(3-‐4): p.245-‐252. (2005)

135. Kuang M, Liu S, Saijo K, Uchimura E, Huang L, Leong K, Lu M, Huang J, Ohno T. Microwave tumour coagulation plus in situ treatment with cytokine-‐microparticles: Induction of potent anti-‐residual tumour immunity. International Journal of Hyperthermia, 21(3): p.247-‐257. (2005)

136. Mao H, Shipanova-‐Kadiyala I, Zhao Z, Dang W, Brown A, Leong K. Biodegradable poly(terephthalate-‐co-‐phosphate)s: Synthesis, characterization and drug-‐release properties. Journal of Biomaterials Science-‐Polymer Edition, 16(2): p.135-‐161. (2005)

137. Yim E, Leong K. Proliferation and differentiation of human embryonic germ cell derivatives in bioactive polymeric fibrous scaffold. Journal of Biomaterials Science-‐Polymer Edition, 16(10): p.1193-‐1217. (2005)

138. Chia S, Lin P, Quek C, Yin C, Mao H, Leong K, Xu X, Goh C, Ng M, Yu H. Engineering microenvironment for expansion of sensitive anchorage-‐dependent mammalian cells. Journal of Biotechnology, 118(4): p.434-‐447. (2005)

139. Liao I, Wan A, Yim E, Leong K. Controlled release from fibers of polyelectrolyte complexes. Journal of Controlled Release, 104(2): p.347-‐358. (2005)


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140. Zhang X, Wang X, Zhang P, Liu Z, Zhuo R, Mao H, Leong K. Galactosylated ternary DNA/polyphosphoramidate nanoparticles mediate high gene transfection efficiency in hepatocytes. Journal of Controlled Release, 102(3): p.749-‐763. (2005)

141. Hu W, Yim E, Reano R, Leong K, Pang S. Effects of nanoimprinted patterns in tissue-‐culture polystyrene on cell behavior. Journal of Vacuum Science & Technology B, 23(6): p.2984-‐2989. (2005)

142. Li J, Ni X, Li X, Tan N, Lim C, Ramakrishna S, Leong K. Micellization phenomena of biodegradable amphiphilic triblock copolymers consisting of poly(beta-‐hydroxyalkanoic acid) and poly(ethylene oxide). Langmuir, 21(19): p.8681-‐8685. (2005)

143. Chen H, Le Visage C, Qiu B, Du X, Ouwerkerk R, Leong K, Yang X. Mr imaging of biodegradable polymeric microparticles: A potential method of monitoring local drug delivery. Magnetic Resonance in Medicine, 53(3): p.614-‐620. (2005)

144. Salem A, Hung C, Kim T, Wu T, Searson P, Leong K. Multi-‐component nanorods for vaccination applications. Nanotechnology, 16(4): p.484-‐487. (2005)

145. Mao H, Leong K. Design of polyphosphoester-‐DNA nanoparticles for non-‐viral gene delivery. Non-‐Viral Vectors For Gene Therapy, 2nd Edition: Part 1, 53: p.275-‐306. (2005)

146. Kim M, Hwang N, Lee J, Kim T, Leong K, Shamblott M, Gearhart J, Elisseeff J. Musculoskeletal differentiation of cells derived from human embryonic germ cells. Stem Cells, 23(1): p.113-‐123. (2005)

147. Lu H, Lim W, Zhang P, Chia S, Yu H, Mao H, Leong K. Galactosylated poly(vinylidene difluoride) hollow fiber bioreactor for hepatocyte culture. Tissue Engineering, 11(11-‐12): p.1667-‐1677. (2005)

148. Yim E, Wen J, Leong K. Enhanced extracellular matrix production and differentiation of human embryonic germ cell derivatives in biodegradable poly(epsilon-‐caprolactone-‐co-‐ethyl ethylene phosphate) scaffold. Acta Biomaterialia, 2(4): p.365-‐376. (2006)

149. Dang J, Leong K. Natural polymers for gene delivery and tissue engineering. Advanced Drug Delivery Reviews, 58(4): p.487-‐499. (2006)

150. Li J, Yang C, Li H, Wang X, Goh S, Ding J, Wang D, Leong K. Cationic supramolecules composed of multiple oligoethylenimine-‐grafted beta-‐cyclodextrins threaded on a polymer chain for efficient gene delivery. Advanced Materials, 18(22): p.2969-‐+. (2006)

151. Wong K, Sun G, Zhang X, Dai H, Liu Y, He C, Leong K. Pei-‐g-‐chitosan, a novel gene delivery system with transfection efficiency comparable to polyethylenimine in vitro and after liver administration in vivo. Bioconjugate Chemistry, 17(1): p.152-‐158. (2006)

152. Wu D, Liu Y, Jiang X, He C, Goh S, Leong K. Hyperbranched poly(amino ester)s with different terminal amine groups for DNA delivery. Biomacromolecules, 7(6): p.1879-‐1883. (2006)

153. Chua K, Chai C, Lee P, Tang Y, Ramakrishna S, Leong K, Mao H. Surface-‐aminated electrospun nanofibers enhance adhesion and expansion of human umbilical cord blood hematopoietic stem/progenitor cells. Biomaterials, 27(36): p.6043-‐6051. (2006)

154. Dang J, Sun D, Shin-‐Ya Y, Sieber A, Kostuik J, Leong K. Temperature-‐responsive hydroxybutyl chitosan for the culture of mesenchymal stem cells and intervertebral disk cells. Biomaterials, 27(3): p.406-‐418. (2006)

155. Jiang X, Chai C, Zhang Y, Zhuo R, Mao H, Leong K. Surface-‐immobilization of adhesion peptides on substrate for ex vivo expansion of cryopreserved umbilical cord blood cd34(+) cells. Biomaterials, 27(13): p.2723-‐2732. (2006)

156. Li J, Li X, Ni X, Wang X, Li H, Leong K. Self-‐assembled supramolecular hydrogels formed by biodegradable peo-‐phb-‐peo triblock copolymers and alpha-‐cyclodextrin for controlled drug delivery. Biomaterials, 27(22): p.4132-‐4140. (2006)

157. Li Q, Wang J, Shahani S, Sun D, Sharma B, Elisseeff J, Leong K. Biodegradable and photocrosslinkable polyphosphoester hydrogel. Biomaterials, 27(7): p.1027-‐1034. (2006)

158. Luong-‐Van E, Grondahl L, Chua K, Leong K, Nurcombe V, Cool S. Controlled release of heparin from poly(epsilon-‐caprolactone) electrospun fibers. Biomaterials, 27(9): p.2042-‐2050. (2006)

159. Ong S, Dai H, Leong K. Inducing hepatic differentiation of human mesenchymal stem cells in pellet culture. Biomaterials, 27(22): p.4087-‐4097. (2006)


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160. Yim E, Wan A, Le Visage C, Liao I, Leong K. Proliferation and differentiation of human mesenchymal stem cell encapsulated in polyelectrolyte complexation fibrous scaffold. Biomaterials, 27(36): p.6111-‐6122. (2006)

161. Chew S, Wen Y, Dzenis Y, Leong K. The role of electrospinning in the emerging field of nanomedicine. Current Pharmaceutical Design, 12(36): p.4751-‐4770. (2006)

162. Bowman K, Leong K. Chitosan nanoparticles for oral drug and gene delivery. International Journal of Nanomedicine, 1(2): p.117-‐128. (2006)

163. Dai H, Jiang X, Tan G, Chen Y, Torbenson M, Leong K, Mao H. Chitosan-‐DNA nanoparticles delivered by intrabiliary infusion enhance liver-‐targeted gene delivery. International Journal of Nanomedicine, 1(4): p.507-‐522. (2006)

164. Feng Q, Chai C, Jiang X, Leong K, Mao H. Expansion of engrafting human hematopoietic stem/progenitor cells in three-‐dimensional scaffolds with surface-‐immobilized fibronectin. Journal of Biomedical Materials Research Part a, 78A(4): p.781-‐791. (2006)

165. Ho Y, Chen H, Leong K, Wang T. Evaluating the intracellular stability and unpacking of DNA nanocomplexes by quantum dots-‐fret. Journal of Controlled Release, 116(1): p.83-‐89. (2006)

166. Jiang X, Dai H, Leong K, Goh S, Mao H, Yang Y. Chitosan-‐g-‐peg/DNA complexes deliver gene to the rat liver via intrabiliary and intraportal infusions. Journal of Gene Medicine, 8(4): p.477-‐487. (2006)

167. Song R, Liu S, Adams R, Leong K. Enhancing efficacy of hiv gag DNA vaccine by local delivery of gm-‐csf in murine and macaque models. Journal of Interferon and Cytokine Research, 26(6): p.380-‐389. (2006)

168. Tsurushima H, Yoshii Y, Leong K, Ohno T. Targeted tumor cell death induced by autologous tumor-‐specific t lymphocyte recognition of wild-‐type p53-‐derived peptides. Journal of Neuro-‐Oncology, 76(2): p.99-‐104. (2006)

169. Li X, Mya K, Ni X, He C, Leong K, Li J. Dynamic and static light scattering studies on self-‐aggregation behavior of biodegradable amphiphilic poly(ethylene oxide)-‐poly [(r)-‐3-‐hydroxybutyrate]-‐poly(ethylene oxide) triblock copolymers in aqueous solution. Journal of Physical Chemistry B, 110(12): p.5920-‐5926. (2006)

170. Lim S, Liao I, Leong K. Nonviral gene delivery from nonwoven fibrous scaffolds fabricated by interfacial complexation of polyelectrolytes. Molecular Therapy, 13(6): p.1163-‐1172. (2006)

171. Chen H, Leong K. Quantum-‐dots-‐fret nanosensors for detecting unamplified nucleic acids by single molecule detection. Nanomedicine, 1(1): p.119-‐122. (2006)

172. Liao I, Chew S, Leong K. Aligned core-‐shell nanofibers delivering bioactive proteins. Nanomedicine, 1(4): p.465-‐471. (2006)

173. Chew S, Hufnagel T, Lim C, Leong K. Mechanical properties of single electrospun drug-‐encapsulated nanofibres. Nanotechnology, 17(15): p.3880-‐3891. (2006)

174. Bright C, Park Y, Sieber A, Kostuik J, Leong K. In vivo evaluation of plasmid DNA encoding op-‐1 protein for spine fusion. Spine, 31(19): p.2163-‐2172. (2006)

175. Le Visage C, Kim S, Tateno K, Sieber A, Kostuik J, Leong K. Interaction of human mesenchymal stem cells with disc cells -‐ changes in extracellular matrix biosynthesis. Spine, 31(18): p.2036-‐2042. (2006)

176. Le Visage C, Yang S, Kadakia L, Sieber A, Kostuik J, Leong K. Small intestinal submucosa as a potential bioscaffold for intervertebral disc regeneration. Spine, 31(21): p.2423-‐2430. (2006)

177. Zhang Y, Chai C, Jiang X, Teoh S, Leong K. Co-‐culture of umbilical cord blood cd34(+) cells with human mesenchymal stem cells. Tissue Engineering, 12(8): p.2161-‐2170. (2006)

178. Chua K, Tang Y, Quek C, Ramakrishna S, Leong K, Mao H. A dual-‐functional fibrous scaffold enhances p450 activity of cultured primary rat hepatocytes. Acta Biomaterialia, 3: p.643-‐650. (2007)

179. Chew S, Mi R, Hoke A, Leong K. Aligned protein-‐polymer composite fibers enhance nerve regeneration: A potential tissue-‐engineering platform. Advanced Functional Materials, 17(8): p.1288-‐1296. (2007)

180. Dang J, Leong K. Myogenic induction of aligned mesenchymal stem cell sheets by culture on thermally responsive electrospun nanofibers. Advanced Materials, 19: p.2775-‐+. (2007)

181. Bursac N, Loo Y, Leong K, Tung L. Novel anisotropic engineered cardiac tissues: Studies of electrical propagation. Biochemical and Biophysical Research Communications, 361(4): p.847-‐853. (2007)


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182. Chen B, Dang J, Tan T, Fang N, Chen W, Leong K, Chan V. Dynamics of smooth muscle cell deadhesion from thermosensitive hydroxybutyl chitosan. Biomaterials, 28(8): p.1503-‐1514. (2007)

183. Tsurushima H, Yuan X, Dillehay L, Leong K. Radioresponsive tumor necrosis factor-‐related apoptosisinducing ligand (trail) gene therapy for malignant brain tumors. Cancer Gene Therapy, 14(8): p.706-‐716. (2007)

184. Tsurushima H, Yuan X, Dillehay L, Leong K. Radio-‐responsive gene therapy for malignant glioma cells without the radiosensitive promoter: Caspase-‐3 gene therapy combined with radiation. Cancer Letters, 246(1-‐2): p.318-‐323. (2007)

185. Yim E, Pang S, Leong K. Synthetic nanostructures inducing differentiation of human mesenchymal stem cells into neuronal lineage. Experimental Cell Research, 313(9): p.1820-‐1829. (2007)

186. Chua K, Chai C, Lee P, Ramakrishna S, Leong K, Mao H. Functional nanofiber scaffolds with different spacers modulate adhesion and expansion of cryopreserved umbilical cord blood hematopoietic stem/progenitor cells. Experimental Hematology, 35(5): p.771-‐781. (2007)

187. Park D, Choi J, Leong K, Kwon J, Eun H. Tissue-‐engineered bone formation with gene transfer and mesenchymal stem cells in a minimally invasive technique. Laryngoscope, 117(7): p.1267-‐1271. (2007)

188. Zhang Y, Chai C, Jiang X, Teoh S, Leong K. Fibronectin immobilized by covalent conjugation or physical adsorption shows different bioactivity on aminated-‐pet. Materials Science & Engineering C-‐Biomimetic and Supramolecular Systems, 27(2): p.213-‐219. (2007)

189. Chai C, Leong K. Biomaterials approach to expand and direct differentiation of stem cells. Molecular Therapy, 15(3): p.467-‐480. (2007)

190. Song R, Liu S, Leong K. Effects of mip-‐1 alpha, mip-‐3 alpha, and mip-‐3 beta on the induction of hiv gag-‐specific immune response with DNA vaccines. Molecular Therapy, 15(5): p.1007-‐1015. (2007)

191. Sharma B, Williams C, Kim T, Sun D, Malik A, Khan M, Leong K, Elisseeff J. Designing zonal organization into tissue-‐engineered cartilage. Tissue Engineering, 13(2): p.405-‐414. (2007)

192. Yim E, Liao I, Leong K. Tissue compatibility of interfacial polyelectrolyte complexation fibrous scaffold: Evaluation of blood compatibility and biocompatibility. Tissue Engineering, 13(2): p.423-‐433. (2007)

193. Chew S, Mi R, Hoke A, Leong K. The effect of the alignment of electrospun fibrous scaffolds on schwann cell maturation. Biomaterials, 29(6): p.653-‐661. (2008)

194. Choi J, Leong K, Yoo H. In vivo wound healing of diabetic ulcers using electrospun nanofibers immobilized with human epidermal growth factor (egf). Biomaterials, 29(5): p.587-‐596. (2008)

195. Chalut K, Chen S, Finan J, Giacomelli M, Guilak F, Leong K, Wax A. Label-‐free, high-‐throughput measurements of dynamic changes in cell nuclei using angle-‐resolved low coherence interferometry. Biophysical Journal, 94(12): p.4948-‐4956. (2008)

196. Tan S, Pan W, Ma G, Cai N, Leong K, Liao K. Viscoelastic behaviour of human mesenchymal stem cells. Bmc Cell Biology, 9. (2008)

197. Liao I, Liu J, Bursac N, Leong K. Effect of electromechanical stimulation on the maturation of myotubes on aligned electrospun fibers. Cellular and Molecular Bioengineering, 1(2-‐3): p.133-‐145. (2008)

198. Chan B, Leong K. Scaffolding in tissue engineering: General approaches and tissue-‐specific considerations. European Spine Journal, 17: p.S467-‐S479. (2008)

199. Tsurushima H, Yuan X, Dillehay L, Leong K. Radiation-‐inducible caspase-‐8 gene therapy for malignant brain tumors. International Journal of Radiation Oncology Biology Physics, 71(2): p.517-‐525. (2008)

200. Bowman K, Sarkar R, Raut S, Leong K. Gene transfer to hemophilia a mice via oral delivery of fviii-‐chitosan nanoparticles. Journal of Controlled Release, 132(3): p.252-‐259. (2008)

201. Chen H, Ho Y, Jiang X, Mao H, Wang T, Leong K. Quantitative comparison of intracellular unpacking kinetics of polyplexes by a model constructed from quantum dot-‐fret. Molecular Therapy, 16(2): p.324-‐332. (2008)

202. Haider M, Cappello J, Ghandehari H, Leong K. In vitro chondrogenesis of mesenchymal stem cells in recombinant silk-‐elastinlike hydrogels. Pharmaceutical Research, 25(3): p.692-‐699. (2008)

203. Chakraborty S, Liao I, Adler A, Leong K. Electrohydrodynamics: A facile technique to fabricate drug delivery systems. Advanced Drug Delivery Reviews, 61(12): p.1043-‐1054. (2009)


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204. Yow S, Quek C, Yim E, Lim C, Leong K. Collagen-‐based fibrous scaffold for spatial organization of encapsulated and seeded human mesenchymal stem cells. Biomaterials, 30(6): p.1133-‐1142. (2009)

205. Lou Y, Peng Y, Chen B, Wang L, Leong K. Poly(ethylene imine)-‐g-‐chitosan using ex-‐810 as a spacer for nonviral gene delivery vectors. Journal of Biomedical Materials Research Part a, 88A(4): p.1058-‐1068. (2009)

206. Liao I, Chen S, Liu J, Leong K. Sustained viral gene delivery through core-‐shell fibers. Journal of Controlled Release, 139(1): p.48-‐55. (2009)

207. Kunder C, John A, Li G, Leong K, Berwin B, Staats H, Abraham S. Mast cell-‐derived particles deliver peripheral signals to remote lymph nodes. Journal of Experimental Medicine, 206(11): p.2455-‐2467. (2009)

208. Chen H, Ho Y, Jiang X, Mao H, Wang T, Leong K. Simultaneous non-‐invasive analysis of DNA condensation and stability by two-‐step qd-‐fret. Nano Today, 4(2): p.125-‐134. (2009)

209. Ho Y, Chen H, Leong K, Wang T. The convergence of quantum-‐dot-‐mediated fluorescence resonance energy transfer and microfluidics for monitoring DNA polyplex self-‐assembly in real time. Nanotechnology, 20(9). (2009)

210. Oney S, Lam R, Bompiani K, Blake C, Quick G, Heidel J, Liu J, Mack B, Davis M, Leong K, Sullenger B. Development of universal antidotes to control aptamer activity. Nature Medicine, 15(10): p.1224-‐U157. (2009)

211. Kulangara K, Leong K. Substrate topography shapes cell function. Soft Matter, 5(21): p.4072-‐4076. (2009) 212. Jiang X, Zheng Y, Chen H, Leong K, Wang T, Mao H. Dual-‐sensitive mice liar nanoparticles regulate DNA

unpacking and enhance gene-‐delivery efficiency. Advanced Materials, 22(23): p.2556-‐2560. (2010) 213. Chen S, Jones J, Xu Y, Low H, Anderson J, Leong K. Characterization of topographical effects on macrophage

behavior in a foreign body response model. Biomaterials, 31(13): p.3479-‐3491. (2010) 214. Yim E, Darling E, Kulangara K, Guilak F, Leong K. Nanotopography-‐induced changes in focal adhesions,

cytoskeletal organization, and mechanical properties of human mesenchymal stem cells. Biomaterials, 31(6): p.1299-‐1306. (2010)

215. Wu Y, Liao I, Kennedy S, Du J, Wang J, Leong K, Clark R. Electrosprayed core-‐shell microspheres for protein delivery. Chemical Communications: p.4743-‐4745. (2010)

216. Kadiyala I, Loo Y, Roy K, Rice J, Leong K. Transport of chitosan-‐DNA nanoparticles in human intestinal m-‐cell model versus normal intestinal enterocytes. European Journal of Pharmaceutical Sciences, 39(1-‐3): p.103-‐109. (2010)

217. Grigsby C, Leong K. Balancing protection and release of DNA: Tools to address a bottleneck of non-‐viral gene delivery. Journal of the Royal Society Interface, 7: p.S67-‐S82. (2010)

218. Zhao F, Veldhuis J, Duan Y, Yang Y, Christoforou N, Ma T, Leong K. Low oxygen tension and synthetic nanogratings improve the uniformity and stemness of human mesenchymal stem cell layer. Molecular Therapy, 18(5): p.1010-‐1018. (2010)

219. Adler A, Leong K. Emerging links between surface nanotechnology and endocytosis: Impact on nonviral gene delivery. Nano Today, 5(6): p.553-‐569. (2010)

220. Phua K, Leong K. Microscale oral delivery devices incorporating nanoparticles. Nanomedicine, 5(2): p.161-‐163. (2010)

221. Ho Y, Leong K. Quantum dot-‐based theranostics. Nanoscale, 2(1): p.60-‐68. (2010) 222. Staats H, Leong K. Polymer hydrogels chaperoning vaccines. Nature Materials: p.537-‐538. (2010) 223. Christoforou N, Oskouei B, Esteso P, Hill C, Zimmet J, Bian W, Bursac N, Leong K, Hare J, Gearhart J.

Implantation of mouse embryonic stem cell-‐derived cardiac progenitor cells preserves function of infarcted murine hearts. Plos One: p.-‐. (2010)

224. Chalut K, Kulangara K, Giacomelli M, Wax A, Leong K. Deformation of stem cell nuclei by nanotopographical cues. Soft Matter, 6(8): p.1675-‐1681. (2010)

225. Yang Y, Leong K. Nanoscale surfacing for regenerative medicine. Wiley Interdisciplinary Reviews-‐Nanomedicine and Nanobiotechnology: p.478-‐495. (2010)


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226. Juul S, Ho YP, Koch J, Andersen FF, Stougaard M, Leong KW, Knudsen BR. Detection of single enzymatic events in rare or single cells using microfluidics. ACS Nano, 5(10): p.8305-‐10. (2011)

227. Sun TM, Du JZ, Yao YD, Mao CQ, Dou S, Huang SY, Zhang PZ, Leong KW, Song EW, Wang J. Simultaneous delivery of sirna and paclitaxel via a "two-‐in-‐one" micelleplex promotes synergistic tumor suppression. ACS Nano, 5(2): p.1483-‐94. (2011)

228. Le DM, Kulangara K, Adler AF, Leong KW, Ashby VS. Dynamic topographical control of mesenchymal stem cells by culture on responsive poly(epsilon-‐caprolactone) surfaces. Adv Mater, 23(29): p.3278-‐83. (2011)

229. Adler AF, Speidel AT, Christoforou N, Kolind K, Foss M, Leong KW. High-‐throughput screening of microscale pitted substrate topographies for enhanced nonviral transfection efficiency in primary human fibroblasts. Biomaterials, 32(14): p.3611-‐9. (2011)

230. Liao IC, Leong KW. Efficacy of engineered fviii-‐producing skeletal muscle enhanced by growth factor-‐releasing co-‐axial electrospun fibers. Biomaterials, 32(6): p.1669-‐77. (2011)

231. Liau B, Christoforou N, Leong KW, Bursac N. Pluripotent stem cell-‐derived cardiac tissue patch with advanced structure and function. Biomaterials, 32(35): p.9180-‐7. (2011)

232. Juul S, Ho YP, Stougaard M, Koch J, Andersen FF, Leong KW, Knudsen BR. Microfluidics-‐mediated isothermal detection of enzyme activity at the single molecule level. Conf Proc IEEE Eng Med Biol Soc, 2011: p.3258-‐61. (2011)

233. Batinic-‐Haberle I, Rajic Z, Tovmasyan A, Reboucas JS, Ye X, Leong KW, Dewhirst MW, Vujaskovic Z, Benov L, Spasojevic I. Diverse functions of cationic mn(iii) n-‐substituted pyridylporphyrins, recognized as sod mimics. Free Radic Biol Med, 51(5): p.1035-‐53. (2011)

234. Spasojevic I, Kos I, Benov LT, Rajic Z, Fels D, Dedeugd C, Ye X, Vujaskovic Z, Reboucas JS, Leong KW, Dewhirst MW, Batinic-‐Haberle I. Bioavailability of metalloporphyrin-‐based sod mimics is greatly influenced by a single charge residing on a mn site. Free Radic Res, 45(2): p.188-‐200. (2011)

235. Ye X, Fels D, Tovmasyan A, Aird KM, Dedeugd C, Allensworth JL, Kos I, Park W, Spasojevic I, Devi GR, Dewhirst MW, Leong KW, Batinic-‐Haberle I. Cytotoxic effects of mn(iii) n-‐alkylpyridylporphyrins in the presence of cellular reductant, ascorbate. Free Radic Res, 45(11-‐12): p.1289-‐306. (2011)

236. Dai H, Jiang X, Leong KW, Mao HQ. Transient depletion of kupffer cells leads to enhanced transgene expression in rat liver following retrograde intrabiliary infusion of plasmid DNA and DNA nanoparticles. Hum Gene Ther, 22(7): p.873-‐8. (2011)

237. Chalut KJ, Kulangara K, Wax A, Leong KW. Stem cell differentiation indicated by noninvasive photonic characterization and fractal analysis of subcellular architecture. Integr Biol (Camb), 3(8): p.863-‐7. (2011)

238. Li HY, LaBean TH, Leong KW. Nucleic acid-‐based nanoengineering: Novel structures for biomedical applications. Interface Focus, 1(5): p.702-‐724. (2011)

239. Yang Y, Kulangara K, Sia J, Wang L, Leong KW. Engineering of a microfluidic cell culture platform embedded with nanoscale features. Lab Chip, 11(9): p.1638-‐46. (2011)

240. Wu Y, Ho YP, Mao Y, Wang X, Yu B, Leong KW, Lee LJ. Uptake and intracellular fate of multifunctional nanoparticles: A comparison between lipoplexes and polyplexes via quantum dot mediated forster resonance energy transfer. Mol Pharm, 8(5): p.1662-‐8. (2011)

241. Ho YP, Grigsby CL, Zhao F, Leong KW. Tuning physical properties of nanocomplexes through microfluidics-‐assisted confinement. Nano Lett, 11(5): p.2178-‐82. (2011)

242. Yow SZ, Lim TH, Yimi EKF, Lim CT, Leong KW. A 3d electroactive polypyrrole-‐collagen fibrous scaffold for tissue engineering. Polymers, 3: p.527-‐544. (2011)

243. Lee J, Sohn JW, Zhang Y, Leong KW, Pisetsky D, Sullenger BA. Nucleic acid-‐binding polymers as anti-‐inflammatory agents. Proc Natl Acad Sci U S A, 108(34): p.14055-‐60. (2011)

244. Yuan M, Leong KW, Chan BP. Three-‐dimensional culture of rabbit nucleus pulposus cells in collagen microspheres. Spine J, 11(10): p.947-‐60. (2011)

245. Kolind K, Leong KW, Besenbacher F, Foss M. Guidance of stem cell fate on 2d patterned surfaces. Biomaterials, 33(28): p.6626-‐33. (2012)


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246. Kulangara K, Yang Y, Yang J, Leong KW. Nanotopography as modulator of human mesenchymal stem cell function. Biomaterials, 33(20): p.4998-‐5003. (2012)

247. Ma G, Petersen E, Leong KW, Liao K. Mechanical behavior of human embryonic stem cell pellet under unconfined compression. Biomech Model Mechanobiol, 11(5): p.703-‐14. (2012)

248. Pisetsky DS, Lee J, Leong KW, Sullenger BA. Nucleic acid-‐binding polymers as anti-‐inflammatory agents: Reducing the danger of nuclear attack. Expert Rev Clin Immunol, 8(1): p.1-‐3. (2012)

249. Loo Y, Grigsby CL, Yamanaka YJ, Chellappan MK, Jiang X, Mao HQ, Leong KW. Comparative study of nanoparticle-‐mediated transfection in different gi epithelium co-‐culture models. J Control Release, 160(1): p.48-‐56. (2012)

250. Yang S, Guo F, Kiraly B, Mao X, Lu M, Leong KW, Huang TJ. Microfluidic synthesis of multifunctional Janus particles for biomedical applications. Lab Chip, 12(12): p.2097-‐102. (2012)

251. Park S, Son YJ, Leong KW, Yoo HS. Therapeutic nanorods with metallic multi-‐segments: Thermally inducible encapsulation of doxorubicin for anti-‐cancer therapy. Nano Today, 7(2): p.76-‐84. (2012)

252. Grigsby CL, Ho YP, Leong KW. Understanding nonviral nucleic acid delivery with quantum dot-‐fret nanosensors. Nanomedicine (Lond), 7(4): p.565-‐77. (2012)

253. St John AL, Chan CY, Staats HF, Leong KW, Abraham SN. Synthetic mast-‐cell granules as adjuvants to promote and polarize immunity in lymph nodes. Nat Mater, 11(3): p.250-‐7. (2012)

254. Stearns NA, Lee J, Leong KW, Sullenger BA, Pisetsky DS. The inhibition of anti-‐DNA binding to DNA by nucleic acid binding polymers. PLoS One, 7(7): p.e40862. (2012)

255. Jain S, Pitoc GA, Holl EK, Zhang Y, Borst L, Leong KW, Lee J, Sullenger BA. Nucleic acid scavengers inhibit thrombosis without increasing bleeding. Proc Natl Acad Sci U S A, 109(32): p.12938-‐43. (2012)

256. Juul S, Nielsen CJ, Labouriau R, Roy A, Tesauro C, Jensen PW, Harmsen C, Kristoffersen EL, Chiu YL, Frohlich R, Fiorani P, Cox-‐Singh J, Tordrup D, Koch J, Bienvenu AL, Desideri A, Picot S, Petersen E, Leong KW, Ho YP, Stougaard M, Knudsen BR. Droplet microfluidics platform for highly sensitive and quantitative detection of malaria-‐causing plasmodium parasites based on enzyme activity measurement. ACS Nano. (2012)

257. Choy AT, Leong KW, Chan BP. Chemical modification of collagen improves glycosaminoglycan retention of their co-‐precipitates. Acta Biomater. (2012)

258. Wang X, Sherman A, Liao G, Leong KW, Daniell H, Terhorst C, Herzog RW. Mechanism of oral tolerance induction to therapeutic proteins. Adv Drug Deliv Rev. (2012)

259. Peters EB, Christoforou N, Leong KW, Truskey GA. Comparison of mixed and lamellar coculture spatial arrangements for tissue engineering capillary networks in vitro. Tissue Eng Part A. (2012)

260. Zhang Y, Chan HF, Leong KW. Advanced materials and processing for drug delivery: The past and the future. Adv Drug Deliv Rev. (2012)

261. Diekman BO, Christoforou N, Willard VP, Sun H, Sanchez-‐Adams J, Leong KW, Guilak F. Cartilage tissue engineering using differentiated and purified induced pluripotent stem cells. Proc Natl Acad Sci U S A, 109(47): p.19172-‐7. (2012)

262. Yang Y, Kulangara K, Lam RT, Dharmawan R, Leong KW. Effects of topographical and mechanical property alterations induced by oxygen plasma modification on stem cell behavior. ACS Nano, 6(10): p.8591-‐8. (2012)

Reviews and Book Chapters Dziemianowicz, T., Leong, K., and Forsman, W., "Isothermal desorption of graphite nitrate." in Intercalated Graphite,

Dresselhaus, M., Ed., 277, Elsevier Science Publishing, 1983 Langer, R., Siegel, R., Brown, L., Leong, K., Kost, J., and Edelman, E., "Controlled release and magnetically modulated

systems for macromolecular drugs." Annals New York Acad. Sci., 446, 1, 1984 Kost, J., Leong, K., and Langer, R., "Ultrasonic modulated drug delivery system.” in Polymers in medicine: Biomedical

and pharmaceutical applications, Chiellini, E., Ed., Plenum Press, p.387, 1986


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Langer, R., Siegel, R., Brown, L., Leong, K., Kost, J., and Edelman, E., "Controlled release: Three mechanism." Chemtech, 16, 108, 1986

Leong, K., "Polymers for controlled release of drugs." ASTM Standardization News on Advanced Materials, 14, 50, 1986

Rosen, H., Kohn, J., Leong, K., and Langer, R., "Bioerodible polymers for controlled release systems.” in Controlled release systems: Fabrication technology, Hsieh, D., Ed., CRC Press, Boca Raton, 83, 1988

Leong, K., Domb, A., Ron, E., and Langer, R., "Polyanhydrides.” in Encyclopedia of Polymer Science and Engineering, 2nd Ed., p.648, 1990

Tashev, E., Shi, F.Y., and Leong, K.,"Potential of biodegradable poly(phosphoester-‐urethanes) in controlled drug delivery.” ACS Polymeric Mater Sci Eng, 63, 43, 1990

Chasin, M., Domb, A., Ron, E., Mathiowitz, E., Leong, K., Brem, H., and Langer, R., "Polyanhydrides as drug delivery systems.” in Biodegradable polymers as drug delivery systems, Eds. Langer, R. and Chasin, M., Marcel Dekker, New York, p.43, 1991

Leong, K., "Synthetic bioerodible polymeric drug delivery system.” in Polymers for controlled drug delivery, Ed., Tarcha, P., CRC Press, p. 127, 1991

Lee, D. and Leong, K., "Medicated bioerodible ophthalmic prostheses.” in High performance of biomaterials: A comprehensive guide to medical and pharmaceutical applications, Ed., Szycher, M., Technomic Pub. Co., p.733, 1991

Lo, H., Kadiyala, S., and Leong, K., "Biodegradable polymers as synthetic bone grafts.” AAOS Symposium Bone Formation and Repair, Eds. C. Brighton, G Friedlaender, and J. Lane, AAOS, 317, 1994

Leong, K., ""Chemical and mechanical considerations of biodegradable polymers for orthopedic applications.” in Biodegradable Implants in Fracture Fixation, Eds. Leung, K, L. Hung, and P. Leung, World Scientific, p.45, 1994

Kadiyala S, Lo H, Ponticiello MS, and Leong, K, "Poly(phosphoester)s: Synthesis, physico-‐chemical characterization, and biological response.” in Biomedical Applications of Synthetic Biodegradable Polymers, Ed. J. Hollinger, CRC Press, p.33, 1995

Leong, K, Mao, H, Zhuo, R, "Biodegradable polymers with a phosphoryl-‐containing backbone: Tissue engineering and controlled drug delivery applications.” J. Chin. Polym. Sci., 13, 289, 1996

Leong K, Zhao Z, and Dahiyat B, "New Biodegradable Polymers for Medical Applications: Elastomeric Poly(phosphoester-‐urethane)s." in Controlled Drug Delivery: The Next Generation, Ed. K Park, ACS Reference Book, p.469, 1997

Leong K, "Drug Delivery Related to Tissue Engineering." in Tissue Engineering: Synthetic Biodegradable Polymer Scaffolds," Eds. K Atala and D Mooney, Birkhauser Boston, p.97, 1997

Kadiyala S, Lo H, and Leong K, “Formulation of highly porous polymeric foams with controlled release capability: A phase-‐separation technique.” in Tissue Engineering Methods and Protocols, Eds., J. Morgan and M. Yarmush, 1997

Mao HQ, Zhao Z, Dang W, Shipanova-‐Kadiyala I, Leong KW, “Biodegradable Poly(phosphoester)s.” in Encyclopedia of Controlled Drug Delivery, First Edition, Ed. E. Mathiowitz, Johns Wiley & Sons, Inc. New York, NY, 1999

Leong KW, “Biopolymer-‐DNA nanospheres.” in Non-‐viral Vectors for Gene Therapy, Eds. Huang L, et al, Academic Press, p. 267, 1999

Bursac, N., Loo, Y., Irby, M.E., Leong, KW., Tung, L. (2002) “Polymer Scaffolds for Anisotropic Growth of Engineered Cardiac Tissue.” in Biomedical Engineering: Recent Developments . p. 141. Vossoughi, J. (Editor).

Sun DN, Leong KW, “Functional engineering of Load-‐supporting soft tissues.” in Encyclopedia of Comprehensive Structural Integrity (CSI), eds. Y.W. Mai and S.H. Teoh, 97-‐130, 2003, Elsevier, Oxford, UK.

Mao HQ and Leong KW, “Polymeric scaffolds for tissue engineering.” in Frontiers in Biomedical Engineering, Eds. Huang N and Woo S, p. 395-‐411, Kluwer Academic, 2004

Wen J and Leong KW, “Nanotechnology of synthesis and processing of polymeric controlled gene delivery” in Encyclopedia of Nanoscience and Nanotechnology, ed. Nalwa HS, American Scientific Publishers, Stevenson Ranch, CA, 2004.


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Salem A and Leong KW, “Polymeric scaffolds for gene delivery and regenerative medicine.” in Scaffolding in Tissue Engineering, Eds. Ma P and Elisseeff J, 317-‐334, Taylor and Francis Group, Boca Raton, 2006.

Leong KW, “Design of polymeric gene carriers for nonviral gene delivery.” in BioMEMS and Biomedical Nanotechnology Encyclopedia, Eds. Lee A and Lee J, 239-‐259, Springer Science + Business Media, New York, 2006.

Dunham B, Flint P, Singhal S, Le Visage C, Leong KW, “Tracheal Tissue Engineering,” in Tissue Engineering and Artificial Organs, J. Bronzino, Editor. 2006, CRC Press.

Loo Y and Leong KW (2006), “Biomaterials for drug and gene delivery.” in An Introduction to Biomaterials, Eds. Hollinger J and Guelcher S, p. 341-‐367, CRC Biomedical Engineering Series, Taylor and Francis, New York, 2006.

Leong KW (2005), “Polymeric controlled nucleic acid delivery.” MRS Bulletin, 30(9): 640. Nori A, Yim EFK, Chen S, Leong KW (2006), “Cell-‐substrate interactions in regenerative medicine.” in Principles of

Regenerative Medicine, Eds. Atala A, Lanza R, Thomson JA, Nerem R, Academic Press, San Diego, 2007. Chalut K, Kulangara K, Leong KW. “Cellular response to continuous nanostructures”, in Handbook of Nanophysics:

Nanomedicine and Nanorobotics, S. K.D., Editor. 2010, CRC Press. p. 1-‐18. Phua KKL, Roberts ERH, and Leong KW. (2011) Degradable Polymers. In: P. Ducheyne, K.E. Healy, D.W. Hutmacher,

D.W. Grainger, C.J. Kirkpatrick (eds.) Comprehensive Biomaterials, vol. 1, pp. 381-‐415 Elsevier. Edited Book Polymers in Medicine and Pharmacy, Eds. Mikos AH, Leong, KW, et al, Materials Research Society Symposium Proceedings Series, Volume 394, 1995. Proceeding Papers/Extended Abstracts/Abstracts Leong, K., Forsman, W., Vogel, L. "Intercalation of graphite with adducts of nitrosyl chloride and metal chlorides,"

Extended Abstracts of 15th Biennial Conference on Carbon, 398, 1981 Leong, K. and Forsman, W., "Vapor-‐phase intercalation by AlCl3-‐HCl mixtures,". Extended Abstract of 15th Biennial

Conference on Carbon, 356, 1981 Leong, K., Tome, A., Dziemianowicz, T., and Forsman, W., "Intercalation of transition metal chlorides with nitrogen

oxide as coreagent,". 3rd International Conference on Graphite Intercalation Compounds, Nancy, France 1983 Leong, K. and Langer, R., "Drug delivery systems based on bioerodible polyanhydrides,". Proceedings of 10th

International Symposium on Controlled Release of Bioactive Agents, Heller, J. and Jaffe, H., Eds., 293, 1983 Leong, K., Brott, B.C., and Langer, R., "Bioerodible polyanhydrides as a drug-‐carrier matrix,". Polymer Preprints, 25,

201, 1984 Langer, R, Siegel R, Brown L, Leong K, Kost J, and Edelman E., "Controlled Release Systems -‐ Some Recent Advances."

Abstracts of Papers of the American Chemical Society, 188(AUG): 23-‐Pmse, 1984 Leong, K., Cicuta, A., and Langer, R., "Controlled release of bioactive agents from biodegradable polyanhydride

microcapsules,". Proceedings of 11th International Symposium on Controlled Release of Bioactive Materials, Meyers, W. and Dunn, R., Eds., 135, 1984

Kost, J., Leong, K., and Langer, R., "Ultrasonic controlled polymeric delivery systems,". Proceedings of 11th International Symposium on Controlled Release of Bioactive Materials, Myers, W., and Dunn, R., Eds., 84, 1984

Langer, R., Siegel, R., Brown, L., Leong, K., Kost, J., and Edelman, E., "Controlled release systems: Some recent advances,". Proceedings of ACS Division of Polymeric Materials: Science and Engineering, 51, 115, 1984

Leong, K., Simonte, V., Hilton, J., Colvin, M., Brundrett, B., Brem, H., and Langer, R., "Bioerodible polyanhydrides for cancer chemotherapy,". Proceedings of 12th International Symposium on Controlled Release of Bioactive Materials, Peppas, N. and Haluska, R., Eds., 106, 1985

Leong, K. and Langer, R., "Polyanhydrides for Controlled Drug Delivery,". AICHE, Chicago, 1985


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Kost, J., Leong, K., and Langer, R., "Ultrasonic modulated delivery systems,". 2nd International Conference on Polymers in Medicine, Capri, Italy, C29. 1-‐2, 1985

Kost, J., Leong, K., and Langer, R., "Modulated polymeric controlled drug delivery systems,". 3rd Israel Materials Engineering Conference, Haifa, 1985

Kost, J., Leong, K., and Langer, R., "Ultrasonically controlled delivery systems,". Proceedings of 12th International Symposium on Controlled Release of Bioactive Materials, Peppas, N. and Haluska, R., Eds., 73, 1985

Mathiowitz, E., Leong, K., and Langer, R., "Macromolecular drug release from bioerodible polyanhydride microspheres,". Proceedings of 12th International Symposium on Controlled Release of Bioactive Materials, Peppas, N. and Haluska, R., Eds., 183, 1985

Leong, K. and Langer, R., "Implantable controlled drug delivery,". ACEMB, 222, 1986 Langer, R., Leong, K., Kohn, J., Mathiowitz, E., Wheatley, M., Domb, A., Kost, J., Yu, H., and Laurencin, C., "Novel

biodegradable polymers,". Proceedings of 13th International Symposium on Controlled Release of Bioactive Materials, Chaudry, I. and Thies, C., Eds., 8, 1986

Lee, D.A., Flores, R., Anderson, P.J., and Leong, K., "Filtration surgery in rabbits using slow release polymers and 5-‐FU,". ARVO, 1986

Kost, J., Leong, K., and Langer, R., "Ultrasonically controlled drug delivery in vivo,". Controlled Release Society, 1987 Kost, J., Leong, K., and Langer, R., "Ultrasonically controlled polymeric drug delivery,". IUPAC International

Symposium on Polymers for Advanced Technologies, 1987 Brem, H., Fossett, D., Myseros, J., Leong, K., Tamargo, R., Colvin, M., and Hilton, J., "Sustained local release of a

biologically active chemotherapeutic agents by a new bioerodible polymer,". Annual meeting of Neurosurgery Association, 1987

Tarmargo, R., Leong, K., Brem, H., "Inhibition of growth of 9L glioma by local sustained release of angiogenesis inhibitors,". Amer. Ass. Neurological Surgeons, 1987

Brandt, K., Richards, M., and Leong, K., "Synthesis and properties of poly(phosphates) and poly(phosphonates),". MABEC, Philadelphia, 1988

Li, N., Richards, M., Brandt, K., and Leong, K., "Poly(phosphate esters) as drug-‐carriers,". Polymer Preprint, 30, 454, 1989

Leong, K., Tang, A., Li, N., Meilchen, M., and McHugh, M., "Substrates for study of polymer-‐cell interaction. I: Synthesis and surface modification,". AICHE Symposium on Biomaterials, 1989

Saltzman, M., Leong, K., Wingerter, P., Sobarzo, M., Tang, A., Li, N., and Lin, S., "Fibroblasts and hepatocytes attach to novel polymeric substrates,". ASME Symposium on Tissue Engineering, 1989

Richards, M., Dahiyat, B., Arm, D., Li, N., and Leong, K., "New biodegradable polymers for drug delivery,". AICHE Summer Meeting, Philadelphia, 1989

Jampel, H., Leong, K., Dunkelberger, G., and Quigley, H., "Filtration surgery in the glaucomatous non-‐human primate using polyanhydride disks containing fluorouridine (FUR),". ARVO, 1989

Leong, K., Dahiyat, B., and Mostwin, J., "Polymeric controlled delivery of oxybutinin and lidocaine to the bladder,". Am. Urological Ass. Annual Meeting, Dallas, 1989

Leong, K., "Poly(phosphoesters) as Biomaterials,". Polymer Preprint, 31, No. 1, 251, 1990 Jampel, H., Leong, K., Koya, P., and Quigley, H., "The use of hydrophobic drugs incorporated into polyanhydrides in

experimental glaucoma surgery," ARVO, 1990 Saltzman, M., Parkhurst, M.R., Parsons-‐Wingerter, P., and Leong, K., "Cell movement on two and three-‐dimensional

substrates," AICHE Annual Meeting, Chicago, 1990 Tang, A., Parsons-‐Wingerter, P., Saltzman, M., Leong, K., "Covalently bound cell-‐specific ligands on styrene based

polymers for possible use as long-‐term cell culture surfaces," AICHE Annual Meeting, Chicago, 1990 Goodwin, L.T., Leong, K., Lee, D.A., Panek, W.C., Christensen, R.E., "The effects of cytosine arabinoside impregnated

bioerodible polymers on glaucoma filtration surgery in rabbits,". ARVO, 1990 Panek, W.C., Hartstein, I., Lee, D.A., Christensen, R.E., Leong, K., "The use of bioerodable polymers and colchicine in

glaucoma filtration surgery,". ARVO, 1990


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Ganthier, R., J. Charles, Wilson, M.R., Lee, D.A., Kitada, S., Glasgow, B., and Leong, K., "The use of biodegradable polymers impregnated with mitomycin C in glaucoma filtration surgery,". ARVO, 1990

Shi, F.Y., Wang, L.F., and Leong, K., "Synthesis and Characterization of hydrolytically labile polyurethanes,". Polymer Preprint, 31, No. 2, 177, 1990

Richards, M., Arm, D.M., Dahiyat, B.I., Brown, P.R., Tang, A.Y., and Leong, K., "Poly(phosphoesters): A class of versatile degradable biomaterials,". AMS for Biomaterials, 1990

Tashev, E, Shi FY, and Leong KW. "Potential Applications of Poly(Phosphoester-‐Urethanes) in Controlled Drug Delivery." Abstracts of Papers of the American Chemical Society, 200: 10-‐Pmse, 1990

Tanner, K., Kadiyala, S., Frondoza, C., and Leong, K., "Effects of biodegradable polymers on macrophage functions in vitro," AMS for Biomaterials, Scottsdale, 1991

Azhari, R. and Leong, K., "Protein release from enzymatically-‐degradable chondroitin-‐sulfate/gelatin microspheres," Proceed. Intern. Symp. Control. Rel. Bioact. Mater., 18, 617, 1991

Saltzman, M., Radomsky, M., Dang, W., Leong, K., and Grossman, S., "Transport of drugs in the brain following release from a polymer," Proceed. Intern. Symp. Control. Rel. Bioact. mater., 18, 197, 1991

Parsons-‐Wingerter, P., Tang, A., Zhu, W., Ouyang, W., Leong, K., and Saltzman, M., "Polymers for three dimensional cell culture," Polymer Preprints, 32, No.2, 231, 1991

Leong, K., "Poly(phosphoesters) as biomaterials: Controlled drug delivery and orthopedic applications," Guilin International Symposium on Biomaterials and Fine Polymers, 33, 1991

Koya, P., Jampel, H., Quigley, H., and Leong, K., "Pharmacokinetics of VP-‐16 release from polyuanhydrides after experimental filtration surgery," ARVO, 1991

Lewis, H., Schwartz, S., Lee, D., and Leong, K., "The use of bioerodible polymer and 5-‐fluorouracil in the treatment of experimental proliferative vitreoretinopathy," ARVO, 1991

Tang, A., Parsons-‐Wingerter, P., Watzman, M., Anderson, C., and Leong, K., "Culture of hepatocytes on microcarriers derivatized with cell-‐specific ligands," AICHE, Los Angeles, 1991

Leong, K., Shi, F., Zhao, Z., and Dahiyat, B., "Synthesis and characterization of poly(phosphoester-‐urethanes), AICHE, Los Angeles, 1991

Kadiyala, S., Guggino, S., Michelson, J., and Leong, K., "Interaction of biodegradable poly(phosphoesters) with bone," AICHE, Los Angeles, 1991

Dahiyat, B., Hostin, E., Cynamon, M., and Leong, K., "Biodegradable polyurethane microparticles for passively targeted delivery of antibiotics," AICHE, Los Angeles, 1991

Dang, W., Radomsky, M., Grossman, S., Leong, K., and Saltzman, M., "Designing drugs for intracranial drug delivery," AICHE, Los Angeles, 1991

Reisfeld, B., Blackband, S., and Leong, K., "Modelling of controlled drug release and transport in the brain," AICHE, Los Angeles, 1991

Vogelsang, GB, Bujnovszky C, Boucher C, Leong K, Jalalian M, Hess A, and Santos GW. "Invitro Immunological Effects of Thalidomide." Experimental Hematology, 19(6): 553-‐553, 1991

Dahiyat, B., Hostin, E., Posadas, E., Cynamon, M., and Leong, K., "Degradable poly(phosphoester-‐urethanes) based on putrescine for medical applications," ACS Polymeric Mater Sci Eng, 67, 87, 1992

Dahiyat, B., Hostin, E., Posadas, E., Cynamon, M., and Leong, K., "Poly(phosphoester-‐urethanes) for drug delivery," Proceed. Intern. Symp. Control. Rel. Bioact. Mater., 19, 1992

Azhari, R. and Leong, K., "Enzymatically-‐triggered release of recombinant soluble complement receptor 1 (sCR1) from chondroitin sulfate/gelatin microspheres," Proceed. Intern. Symp. Control. Rel. Bioact. Mater., 19, 1992

Azhari, R., Hirosue, S., and Leong, K., "Controlled release from gelatin/chondroitin sulfate microspheres," ACS, San Francisco, 1992

Reisfeld, B., Blackband, S., Calhoun, V., Grossman, S., Eller, S., and Leong, K., "Tracking and modeling controlled drug release and transport in the brain," AICHE, Miami, 1992

Tang, A., Zurlo, J., and Leong, K., "Rat hepatocyte functions on lactose derivatized poly(styrene) surfaces," AICHE, Miami, 1992


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Lo, H. and Leong, K., "Synthesis and evaluation of poly(phosphoramidates) as bioerodible materials," AICHE, Miami, 1992

Zhao, Z. and Leong, K., "Controlled release from biodegradable polyurethanes," AICHE, Miami, 1992 Kadiyala, S., Michelson, J. and Leong, K., "Biodegradable poly(phosphoesters) for orthopedic applications," 19th

Annual Meeting for Society of Biomaterials, p.321, 1993 Kalyanasundaram S, Reisfeld B, Calhoun V, and Leong K: "Polymeric controlled release and transport in the brain-‐A

mathematical model," p. 51, Proceedings of International Conference on Controlled Release of Bioactive Agents, Washington D.C, 1993

Truong, V. and Leong, K., "A target-‐specific microspheres drug delivery system made of enzymatically degradable gelatin and chondroitin sulfate coacervates," p. 474, Proceedings of International Conference on Controlled Release of Bioactive Agents, Washington D.C, 1993

Calhoun, V., Kalyanasundaram S, Barker, P., Grossman, S., Eller, S., and Leong, K., "3D T1 Mapping of a contrast agent concentration gradient: Quantifying the transport of an intracranial delivery of Gd-‐DTPA to the parenchyma of a rabbit brain," Soc. MRI Meeting, New York, 1993

Truong, V., Hildreth, J., Mok, P., Freeman, A., and Leong, K., "Targeting of Anti-‐LFA-‐1 mAb coated immuno-‐microspheres to histocytic U937 cell line," Experimental Biology, New Orleans, 1993

Truong, V. and Leong, K., "P-‐glycoprotein-‐specific binding of immuno-‐microspheres to drug-‐resistant KB-‐V-‐1 cells," Experimental Biology, New Orleans, 1993

Leong, K., "Poly(phosphoesters) and poly(phosphoester-‐urethanes) in controlled drug delivery applications," American Society for Artificial Internal Organs, New Orleans, 1993

Leong, K., "Poly(phosphoesters) and poly(phosphoester-‐urethanes) in orthopedic and controlled drug delivery applications," Gordon Conference, San Miniato, Italy, 1993

Uppal, P., Jampel, H., Lee, D., and Leong, K., "Biodegradable implants for glaucoma filtration surgery," Symposium on Ocular Pharmacology, Novi, 1993

Truong, V., Calhoun, V., Williams, J., Hildreth, J., and Leong, K., "Drug delivery using bio-‐polymeric immuno-‐microspheres: Active targeting of porphyrins, radiopharmaceutics, and MRI contrast agents in vitro and in vivo," Science Innovation 93, Boston, 1993

Brown, K., Shao, W., Bathon, J., and Leong, K., "Evaluation of drug-‐loaded microspheres in synovial fluids," Soc. Rheumatology, San Antonio, 1993

He, W., Vogelsang, G., Leong, K., "Synthesis of thalidomide analogs and their biological potential in graft-‐versus-‐host disease," ACS Fall Meeting, Chicago, 1993

Leong, K., "Designing polymers for controlled drug delivery," Science Innovation 93, Boston, 1993 Leong, K., "Alternative rodding materials for osteogenesis imperfecta," Symposium on Osteogenesis Imperfecta,

Bethesda, 1993 Leong, K., "Chemical and mechanical considerations of biodegradable polymers for orthopedic applications,"

International Soc. Fracture Repair Workshop, Hong Kong, 1993 Gutsche, A.T., Zurlo, J., Leong, K., "Synthesis and fabrication of polymer substrates for hepatocyte culture," Materials

Research Society Annual Meeting, Boston, 1993 Brown, K., Shao, W., Bathon, J., and Leong, K., "Controlled drug delivery to the joints by enzymatically degradable

microspheres," Materials Research Society Annual Meeting, Boston, 1993 Lo, H., Kadiyala, S., Guggino, S., and Leong, K., "Biodegradable microcellular foams for cell transplantation," Materials

Research Society Annual Meeting, Boston, 1993 Jampel, HD, Thibault D, Uppal P, Quigley H, and Leong K. "Glaucoma Filtration Surgery in Nonhuman-‐Primates Using

Taxol and Etoposide in Polyanhydride Carriers." Investigative Ophthalmology & Visual Science, 34(4): 817-‐817, 1993

Truong, VL, Hildreth JE, Mok PKY, Freeman A, and Leong KW. "Targeting of Anti-‐Lfa-‐1 Mab Coated Immuno-‐Microspheres to Histiocytic U937 Cell-‐Line." Faseb Journal, 7(3): A364-‐A364, 1993

Jenkins, GM, Leong K, Heller P, Sollott S, Kinsella J, Nater C, Oconnor F, Froehlich J, and Cheng L. "Local-‐Delivery of


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Taxol Inhibits Neointimal Regrowth Following Balloon Injury of the Rat Carotid-‐Artery." Circulation, 90(4): 297-‐297, 1994

Shao, W., Jaffee, E., Pardoll, D., and Leong, K., "Delivery of cytokines by microencapsulated transduced cells," Proceedings of International Conference on Controlled Release of Bioactive Agents, Nice, France, 1994

Truong, V., Guarnieri, F., Williams, J., August, T., Leong, K., "Immuno-‐microsphere as gene delivery vehicle: Targeting of LAMP-‐1 to lysosomal membrane, " Proceedings of International Conference on Controlled Release of Bioactive Agents, Nice, France, 1994

Shao W, Gutsche A, Sachs J, and Leong K, "Encapsulation of cells by complex coacervatin of collagen and synthetic polyanions," Transactions of Society for Biomaterials, 17, 316, 1994

Leong K, "Microspheres and microcapsules for gene delivery and cell encapsulation," Preprints of International Symposium on Biomaterials and Fine Polymers, Wuhan, 1994

Kadiyala S, Michelson M, and Leong K, "Effect of biodegradable polymers on enchondral ossification: in vivo culture in diffusion chambers," Transactions of Society for Biomaterials, 17, 307, 1994

Lesser, G., Grossman, S., Lo, H., Leong, K., and Eller, S., "In vitro and in vivo studies of subcutaneous hydromorphone implants designed for the treatment of cancer pain," ASCO Annual Meeting, Dallas, 1994

Kalyanasundaram S, Calhoun, V., and Leong, K., "Coupled diffusive-‐convective mass transport in the brain", FASEB, Anaheim, 1994

Lo, H, Kadiyala S, Guggino SE, Reddi AH, and Leong K, "Design of synthetic bone graft: BMP-‐containing biodegradable polymeric foam," International Conf. on Bone Morphogenetic Proteins, Baltimore, MD 1994

Chand D, Gutsche AT, Young TA, Yager JD, and Leong K, "Designing substrates for hepatocytes culture: Correlatin of mRNA expression and enzymatic activity," BMES Annual Meeting, Tucson, 1994

Ponticiello MS, Lo H, Kadiyala, S, and Leong K, "Mechanical reinforcement of biodegradable polymeric foams for use as tissue scaffolds," Transactions of Society for Biomaterials, 18, 62, 1995

Ponticiello MS, Huber MR, Cai H, Bao G, and Leong K, "Experimental and theoretical analysis of a totally resorbable glass-‐fiber reinforced polymeric composite," Transactions of Society for Biomaterials, 18, 378, 1995

Truong VL, Walsh SM, August T, and Leong K, "Gene transfer by gelatin-‐DNA coacervate," Proceedings of International Conference on Controlled Release of Bioactive Agents, Seattle, p.466, 1995

Zhao Z, Jaffee EM, Thomas MC, Pardoll DM, and Leong K, "Controlled delivery of cytokines for therapeutic cancer vaccination," Proceedings of International Conference on Controlled Release of Bioactive Agents, Seattle, p.51, 1995

Zhao, Z, Jaffee E, Pardoll D, and Leong K. "Cytokine Delivery by Biodegradable Beads as Alternative to Gene-‐Transduced Tumors for Augmenting Antitumor Immune-‐Responses." Journal of Cellular Biochemistry: 183-‐183, 1995

Zhao A, Jaffee E, Pardoll D, and Leong K, "Cytokine delivery by biodegradable beads as alternative to gene-‐transduced tumors for augmenting anti-‐tumor immune responses," Keystone Conference on Cancer Immunology, Taos, New Mexico, 1995

Leong K., "Drug delivery to the joint," Keystone Conference on Drug Delivery, Hilton Head, 1995 Leong K., "A synthetic CFTR gene delivery system," Cystic Fibrosis Foundation Conference, Williamsburg, 1995 Leong K., "Delivery of cytokines by gelatin/chondroitin sulfate microspheres," ACS Conference, Boston, 1995 Kadiyala S, Lo H, Ponticiello MS, Reddi AH, and Leong K, "Bone induction achieved by controlled release of BMP from

PLA/Hydroxyapatite foams." Fifth World Biomaterials Congress, Toronto, Canada, 1996 Mao HQ, Roy SM, Walsh JT, August T, and Leong K, "DNA-‐Chitosan nanospheres for gene delivery," Proceedings of

International Conference on Controlled Release of Bioactive Agents, Kyoto, Japan, 1996 Sills AK, Zhao Z, Tyler B, Crongeyer J, Leong K, Pardoll D, and Brem H, “Intracranial delivery of interleukin-‐2

prolongs survival in primary and metastatic brain tumor models,” Congress for Neurosurgeons, Montreal, Canada, 1996

Samdani A, Sills AK, Zhao Z, Tyler B, Ewend M, Leong K, and Brem H, “Systemic vaccination with GM-‐CSF microspheres protects against growth of intracranial melanoma,” Congress for Neurosurgeons, Montreal,


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Canada, 1996 Walsh SM, Flotte TR, Beck S, Allen S, Guggino WB, August T, and Leong K, "Delivery of CFTR gene to rabbit airways

by gelatin-‐DNA microspheres," Proceedings of International Conference on Controlled Release of Bioactive Agents, Kyoto, Japan, 1996

Sills AK, Zhao Z, Wessem K, Crongeyer J, Tyler B, Ewend MG, Leong K, Brem H, "Intracranial delivery of interleukin-‐2 by microspheres in the treatment of brain tumors," Proceedings of International Conference on Controlled Release of Bioactive Agents, Kyoto, Japan, 1996

Walsh SM, Flotte TR, Truong-‐Le V, Allen S, Guggino WB, August T, and Leong K, "CF gene therapy by gelatin-‐DNA microspheres," Pediatric Pulmonology, 13, Supplement, 261, 1996

Kalyanasundaram S, Zhao Z, Sills A, Brem H, and Leong K, "Controlled release of immunotherapy: Analysis of intracranial transport of IL-‐2," Proceedings of International Conference on Controlled Release of Bioactive Agents, Kyoto, Japan, 1996

Zhao Z, Jaffee EM, Thomas MC, Pardoll DM, and Leong K, "Adjunct cancer therapy using GM-‐CSF microspheres-‐-‐A mechanistic study of the vaccine site," Proceedings of International Conference on Controlled Release of Bioactive Agents, Kyoto, Japan, 1996

Leong K., "Biodegradable and macroporous polymeric scaffolds," Keystone Conference on Tissue Engineering, Taos, New Mexico, 1996

Leong K, "Controlled release biodegradable scaffolds for bone regeneration," AAAS Annual Meeting, Baltimore, 1996

Zhao A, Jaffee E, Thomas MC, Pardoll D, and Leong K, "Generation of systemic anti-‐tumor immunity using mixture of GM-‐CSF microspheres and irradiated tumor cells," AAAS Annual Meeting, Baltimore, 1996

Leong K, "Adjuvant anti-‐tumor effects of GM-‐CSF polymeric microspheres," US-‐Japan Cancer Cooperative Research Program on The Role of Cytokines in Cancer, Bethesda, 1996

Roy K, Mao HQ, and Leong KW, “Novel DNA-‐chitosan nanospheres as synthetic gene delivery system,” Gene Therapy, Cold Spring Harbor, 1996

Truong VL, August JT, and Leong KW, “DNA-‐gelatin coacervates as a gene delivery vehicle,” Gene Therapy, Cold Spring Harbor, 1996

Walsh S, Flotte T, Truong VL. Beck S, Allen S, Guggino W, August JT, and Leong KW, “Cystic fibrosis gene therapy by gelatin-‐DNA nanospheres,” 10th N. Amer. Cystic Fibrosis Conference, Orlando, 1996

Walsh SM, Flotte TR, Truong VL, Rubenstien R, Beck S, August T, Zeitline P, and Leong K, “Combination of drug and gene delivery by gelatin nanospheres for the treatment of cystic fibrosis," Proceedings of International Conference on Controlled Release of Bioactive Agents, p.75, Stockholm, Sweden, 1997

Walsh SM, Beck S, Rubenstien R, Zeitline P, and Leong K, “Simultaneous delivery of drugs and genes by gelatin nanospheres," Pediatric Pulmonology, 14, Supplement, p.255, 1997

Roy K, Mao HQ, and Leong K, “DNA-‐Chitosan nanospheres: Transfection efficiency and cellular uptake," Proceedings of International Conference on Controlled Release of Bioactive Agents, p.673, Stockholm, Sweden, 1997

Mao HQ, Roy K, Truong-‐Le V, August JR, and Leong K, “DNA-‐Chitosan nanospheres: Derivatization and storage stability," Proceedings of International Conference on Controlled Release of Bioactive Agents, p.671, Stockholm, Sweden, 1997

Walsh SM, Beck S, Truong VL, Rubenstien R, Zeitline P, and Leong K, “Coencapsulation of drugs and genes by gelatin nanospheres for the treatment of cystic fibrosis," Fourth Annual Conference on Artificial Self-‐Assembling Systems for Gene Delivery, Sponsored by Cambridge Healthtech Institute, Newton Upper Falls, MA, 1997

Truong V, Mao HQ, Walsh S, Leong KW, August JT, “Delivery of DNA vaccine using gelatin-‐DNA nanospheres,” Proceedings of International Conference on Controlled Release of Bioactive Agents, p.39, Stockholm, Sweden, 1997

Liu SQ and Leong KW, “Delivery of protein and low-‐molecular weight drug by coacervate of human serum albumin and heparin,” Proceedings of International Conference on Controlled Release of Bioactive Agents, p. 911, Stockholm, Sweden, 1997

Shipanova I, Mao HQ, Liu S, Hendriks BS, Leong KW, “Novel biodegradable polyphosphate paste for protein release,”


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Proceedings of AICHE Meeting, Los Angeles, 1997 Mao HQ, Dang W, Shipanova I, Zhao Z, Cline D, Leong KW, “Design of new biodegradable polymers based on chain-‐

extension of oligomeric lactides by phosphates,” Proceedings of AICHE Meeting, Los Angeles, 1997 Mao HQ, Shipanova I, Hendriks BS, Kao DP, Dang W, Zhao Z, Leong KW, “Biodegradable copolymer for drug delivery:

Poly(phosphate-‐terephthalate)s,” Proceedings of AICHE Meeting, Los Angeles, 1997 Hanes J, Sills AK, Zhao Z, Tyler BM, Leong K, Pardoll D, and Brem H, “Cancer vaccination in the brain: Local cytokine

release from biodegradable polymers overcomes tolerance in experimental brain tumors,” Conference on Formulations and Drug Delivery II, La Jolla, CA, 1997

Leong K. “DNA nanospheres as non-‐viral vectors for gene delivery,” Eighth Conferences on Advances in Drug Delivery, Salt Lake City, Utah, 1997

Leong KW, “Cystic fibrosis gene therapy by gelatin-‐DNA nanospheres,” Cystic Fibrosis Foundation Conference, Williamsburg, 1997

Leong KW, "Biodegradable polymers for gene delivery and tissue engineering applications," Fourth International Symposium on Biomaterials and Fine Polymers, Xian, China 1997

Leong KW, “Application of controlled release technology for optimization of immune response,” NIH Workshop on Interface of Bioengineering and Immunology, Bethesda, 1997

Mao HQ, Hendriks BS, Lin K, Leong KW, “Intramuscular Delivery of LacZ plasmid encapsulated in microspheres composed of biodegradable phosphate chain-‐extended poly(L-‐lactide),” Proceedings of International Symposium on Controlled Release of Bioactive Materials, 25: 203, Las Vegas 1998

Roy K, Mao HQ, Lin K, Lin J, Huang SH, Leong KW, “Oral immunization with DNA-‐chitosan nanospheres,” Proceedings of International Symposium on Controlled Release of Bioactive Materials, 25: 348, Las Vegas 1998

Shipanova-‐Kadiyala I, Liu SQ, Mao HQ, Leong KW, “Polymeric controlled delivery of IL-‐2 and doxorubicin against a murine melanoma model,” Proceedings of International Symposium on Controlled Release of Bioactive Materials, 25: 984, Las Vegas 1998

Roy K, Mao HQ, Leong KW, “Gene delivery with DNA-‐chitosan nanospheres,” Proceedings of the Keystone Symposia on Molecular and Cellular Biology: Synthetic Non-‐Viral Gene Delivery Systems, p143, Keystone, CO, 1998

Liu SQ and Leong KW, “Evaluation of c ytokine-‐containing HAS/Heparin microparticles in generation of tumor-‐spenific CTL and systemic anti-‐tumor immunity,” Proceedings of International Conference on Controlled Release of Bioactive Agents, p. 272, Las Vegas, 1998

Roy K, Lin J, Mao HQ, Huang SK, Leong KW, “Oral delivery of DNA vaccine against food allergy with chitosan-‐DNA nanoparticles,” Gene Therapy, p16, Cold Spring Harbor, NY, 1998

Leong KW, Mao HQ, Walsh SM, Truong-‐Le VL, Roy K, August JT, “Mechanism and efficiency of gene transfection by DNA-‐nanospheres,” Keystone Symposia on Molecular and Cellular Biology: Synthetic Non-‐Viral Gene Delivery Systems, Keystone, CO, 1998

Liu SQ, Aoki H, Ohno T, Leong KW, “Generation of tumor-‐specific cytotoxic T lymphocytes from naive mouse splenocytes in vitro,” Annual Meeting of American Association for Cancer Research, New Orleans, 1998

Liu SQ and Leong KW, “Adjuvant effect of GM-‐CSF HAS/heparin microparticles in cancer immunotherapy,” Proceedings of International Conference on Controlled Release of Bioactive Agents, Boston, 1999

Mao HQ, Lin K, Liu SQ, Haller MF, Leong KW, “Intramuscular Delivery of LacZ plasmid encapsulated in biodegradable poly(D,L-‐lactide-‐co-‐phosphate) microspheres,” Proceedings of International Symposium on Controlled Release of Bioactive Materials, 26, Boston, MA, 1999

Haller MF, Mao HQ, Leong KW, “Co-‐delivery of DNA vaccines and cytokines from a single shot of biodegradable polymer microspheres enhances the antibody response in mice,” The 2nd Annual Meeting of American Society of Gene Therapy, Washington, DC, 1999

Haller MF, Mao HQ, Leong KW, “Intramuscular co-‐delivery of DNA and cytokines from biodegradable microspheres altered immune responses,” Proceedings of the First Joint Meeting of the Biomedical Engineering Society (BMES) and the Engineering in Medicine and Biology Society (EMBS), Atlanta, GA, 1999


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Roy K, Chu W, McGrath J, Kuo S, Huang SK, Leong KW, “Nanoparticle Mediated Oral Gene Delivery for DNA Vaccination,” Capsugel special session on Innovative Aspects of Controlled Drug Release, Proceedings of International Symposium on Controlled Release of Bioactive Materials, 26, Boston, MA, 1999

Chia SM, Li J, Xu X, Gao SJ, Leong KW, Yu H, “Novel Hepatocyte Encapsulation Enhances Cellular Functions,” BMES Annual Meeting, Atlanta, 1999

Mao HQ, Roy K, Lin K, Leong KW, “Polymeric Controlled Delivery of DNA Vaccines,” The Annual Scientific Meeting of Biomedical Research & Experimental Therapeutics Society of Singapore (BRETSS)/Singapore Society of Biochemistry and Molecular Biology (SSBMB)/Singapore Society of Microbiology and Biotechnology (SSMB), Singapore, 1999

Mao HQ, Leong KW, “Design of New Biocompatible Materials for Bioartificial Liver Assist Device,” The 3rd NUH-‐Faculty of Medicine Annual Scientific Meeting, Singapore, 1999.

Liu SQ, Leong KW, “DNA vaccine for cancer,” Annual Meeting of American Association for Cancer Research, Philadelphia, 1999

Roy K, Chu W, McGrath J, Kuo S, Leong KW, “Gene Delivery with Chitosan-‐DNA Nanoparticles: Kinetic and Mechanistic Studies,” American Society of Gene Therapy, Second Annual Meeting, Washington, D.C., 1999

Leong, KW, Mao HQ, Shipanova-‐Kadiyala I, Haller M, Liu SQ, and Li WP. "Biodegradable poly(phosphoester)s." Abstracts of Papers of the American Chemical Society, 217: U567-‐U567, 1999

Leong KW, Roy K, Mao HQ, Truong-‐Le V, August TA, Walsh SM, “Polymeric controlled gene delivery for immunotherapy,” 3rd Congress of European Association for Clinical Pharmacology and Therapeutics, Jerusalem, Israel, 1999

Shi G, Leong KW, “Using Ionene Polymers to Study the Influence of Charge Density of Polycations on Non-‐viral Gene Delivery in vitro,” American Society for Gene Therapy, Denver, 2000

Okoli G, Hortelano G, Leong KW, “Oral gene delivery for hemophilia,” American Society for Gene Therapy, Denver, 2000

Wen J, Mao HQ, Leong KW, “Biodegradable poly(phosphoester)s as gene carriers,” American Society for Gene Therapy, Denver, 2000

Song RJ, Liu S, Adams R, August T, Leong KW, “Adjuvant effect of cytokine microparticles in HIV DNA vaccine design,” Proceedings of International Symposium on Controlled Release of Bioactive Materials, 27, Paris, 2000

Haller M, Sloss D, Wen J, Leong KW, “Polyphosphoester DNA delivery in micro-‐and nanosphere formulations,” Proceedings of International Symposium on Controlled Release of Bioactive Materials, 27, Paris, 2000

Okoli G, Hortelano G, Leong KW, “Oral delivery of Plasmid DNA encoding the factor IX gene,” Proceedings of International Symposium on Controlled Release of Bioactive Materials, 27, Paris, 2000

Kadiyala I, Roy K, Rice J, Leong KW, “Transport kinetics of DNA nanoparticles in a CACO-‐2 Peyer’s patch model of intestinal epithelium,” Proceedings of International Symposium on Controlled Release of Bioactive Materials, 27, Paris, 2000

Wen J, Kim G, Mao, Zhuo R, Leong KW, “New biodegradable polymer for drug delivery system: poly(D,L-‐lactide-‐co-‐ethyl ethylene phosphate),” Proceedings of International Symposium on Controlled Release of Bioactive Materials, 27, Paris, 2000

Li J, Ni X, Zhou Z, Yu H, Leong KW, “Injectable Supramolecular hydrogels for controlled drug delivery,” Proceedings of International Symposium on Controlled Release of Bioactive Materials, 27, Paris, 2000

Roy K, McGrath J, Kuo S, Leong KW, “Chitosan-‐DNA nanoparticles: transfection mechanism and kinetics,” Proceed-‐ings of International Symposium on Controlled Release of Bioactive Materials, 27, Paris, 2000

Kiang T, Wen J, Leong KW, “Effect of degree of deacetylation of chitosan on gene transfection efficiency in vitro,” Proceedings of International Symposium on Controlled Release of Bioactive Materials, 27, Paris, 2000

Mao HQ, Wen J, Lin K, Li W, Leong KW, “Novel biodegradable polyphosphoester micelles as gene carriers,” Proceed-‐ings of International Symposium on Controlled Release of Bioactive Materials, 27, Paris, 2000

Li J, Ni X, Zhou Z, Yu H, Leong KW, “Injectable Supramolecular Hydrogels for Controlled Drug Delivery”, 10th International Conference on Biomedical Engineering, Dec. 6-‐9, 2000; Singapore.

LeVisage C, Malavaud B, Haller M, Leong KW, “Intravesical administration of paclitaxol encapsulated in


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poly(methylidene malonate 2.1.2) microparticles,” BMES Meeting, Seattle, 2000 De la Cruz C, Haller M, Malavaud, B, Leong KW, “Polymeric gene delivery to the lung,” BMES Meeting, Seattle, 2000 Haller M, Malavaud B, LeVisage C, Leong KW, “Bladder instillation of DNA-‐polyphosphoester nanospheres: Transport,

transfection, and immune responses,“ BMES Meeting, Seattle, 2000 Chia, S.M., Xu, X., Li, J., Leong, K.W., Yu, H.,“Double Encapsulation Preserves Enhanced Hepatocyte Functions.” 6th

World Biomaterials Congress, Hawaii, USA, 2000 Chia, S.M., Xu, X., Wan, A.C.A., Leong, K.W., Yu, H. “Living Cell Encapsulation for Tissue Engineering. Signal

Transduction in Engineered Extra Cellular Matrices,” Gordon Research Conference, Tilton School, New Hampshire, USA, 2000

Chia, S.M., Sun, T., Xu, X., Quek, C.H., Chan, M.L.H., Li, J., Leong, K.W., Yu, H. “Optimization of Hepatocyte Micro-‐encapsulation For Bio-‐artificial Liver.” 10th International Conference on Biomedical Engineering, Singapore, 2000

Li J, Zhou Z, Ni X, Yu H, Leong KW, “Pluronic-‐Cyclodextrin based Supramolecular Hydrogels for Controlled Drug Delivery”, 28th International Symposium on Controlled Release of Bioactive Materials, June 23-‐27, 2001; San Diego, USA.

Li J, Zhou Z, Ni X, Li X, Leong KW, “Pluronic-‐Cyclodextrin based Supramolecular Hydrogels for Drug/Gene Delivery and Tissue Engineering”, ICMAT 2001 (International Conference on Materials for Advanced Technologies), Symposium B: Biomaterials and Tissue Engineering, July 1-‐6, 2001, Singapore.

Li J, Li X, Zhou Z, Ni X, Leong KW, “Formation of Supramolecular hydrogels between Pluronic and Cyclodextrin and their Applications for Controlled Drug Delivery”, 5th Asian Symposium on Biomedical Materials, Dec. 9-‐12, 2001, Hong Kong.

Chan, V, Mao, HQ and Leong, KW (2001). "Effect of Chitosan Molecular Weight in Gene Delivery Process." Abstracts of Papers of the American Chemical Society 221: U347-‐U347.

Kumar, M, Behera, AK, Zhang, J, Lockey, RF, Mohapatra, SS, Cruz, CPD, Chen, LC, Lin, J, Leong, KW and Huang, SK (2001). "A Rsv Genome Chitosan Nanosphere (Rgcn) Vaccine against Respiratory Syncytial Virus (Rsv) Infection." Journal of Allergy and Clinical Immunology 107(2): S251-‐S251.

Leong, KW, Wang, J and Mao, HQ (2001). "New Biodegradable and Cationic Poly(Phosphoester)S for Gene Delivery." Abstracts of Papers of the American Chemical Society 222: U419-‐U419.

Chia, S.M., Quek, C.H., Lin, P.C., Mao, H.Q., Leong, K.W., Yu, H. (2001) “A novel 3D cell culture system for tissue engineering applications.” Hilton Head Workshop on Tissue Engineering (ET-‐2001), Hilton Head, USA

Chia, S.M., Lin, P.C., Quek, C.H., Xu, X., Leong, K.W., Yu, H. (2001) “3D co-‐culture of hepatocytes and fibroblasts for liver tissue engineering.” Hilton Head Workshop on Tissue Engineering (ET-‐2001), Hilton Head, USA

Lu H-‐F, WS Lim, P-‐C Zhang, KW Leong, H-‐Q Mao, “Co-‐culture of rat hepatocyte spheroids with 3T3 cells produced high synthetic function,” 17th European Conference on Biomaterials, Barcelona, Spain, September 2002.

Mao H-‐Q, Wang J, Zhang P-‐C, Wang S, Leong KW, “Cationic polyphosphates as gene carriers. 39th International Symposium on Macromolecules” (IUPAC World Polymer Congress 2002), Beijing, China, July 2002.

Lu HF, Mao H-‐Q, Lim WS, Chia SM, Zhang PC, Yu H, Leong KW, Galactosylated PVDF membrane promotes hepatocyte attachment and functional maintenance. 28th Annual Symposium for Biomaterials Society, Tampa, FL, April 2002.

Yin C, Ying L, Zhang PC, Zhuo R-‐X, Kang E-‐T, Leong KW, Mao H-‐Q, “Biofunctional surface with high density galactose ligand enhances hepatocyte attachment and function.” The 29th International Symposium on Controlled Release of Bioactive Materials, Seoul, Korea, July 2002.

Wang J, Huang SW, Zhang PC, Mao H-‐Q, Leong KW, Biodegradable cationic polyphosphoesters for non-‐viral gene delivery. The 29th International Symposium on Controlled Release of Bioactive Materials, Seoul, Korea, July 2002.

Wang J, Zhang PC, Mao H-‐Q, Leong KW, Effects of charge groups of cationic polyphosphoramidates on transfection efficiency. The 5th Annual Meeting of the American Society of Gene Therapy, Boston, MA. June 2002.


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Wang J, Zhang PC, Mao H-‐Q, Leong KW, Mixture of Polyphosphoramidates with Primary Amino Group and Tertiary Amino Group Enhances Transfection Efficiency. The 5th Annual Meeting of the American Society of Gene Therapy, Boston, MA. June 2002.

Leong KW, J Wang, P Zhang, J Wen, S Wang, H Mao, Polymer-‐DNA nanoparticles. The 29th International Symposium on Controlled Release of Bioactive Materials, Seoul, Korea, July 2002.

Yin C, SM Chia, CH Quek, RX Zhuo, KW Leong, H-‐Q Mao. Stable and Semipermeable Hybrid Microcapsules for Cell Encapsulation, 6th NUH-‐NUS Annual Scientific Meeting, NUS, Singapore, August 2002.

Chew JL, Wolfowicz CB, Mao H-‐Q, Hangfu T, Leong KW, Chua KY. House Dust Mite Allergen Gene Der p 1 – Chitosan Nanoparticles Embedded in Jello as a Potential Oral Vaccine for Mite Allergy, 6th NUH-‐NUS Annual Scientific Meeting, NUS, Singapore, August 2002.

Lee I-‐L, J. Wang, SM Chia, H Yu, KW Leong, H-‐Q Mao. Collagen-‐Based Non-‐Viral Gene Carriers, 6th NUH-‐NUS Annual Scientific Meeting, NUS, Singapore, August 2002.

P-‐C Zhang, J Wang, S-‐J Gao, S Wang, KW Leong, H-‐Q Mao. Effects of Charge Groups of Cationic Polyphosphoramidates on Transfection Efficiency. 6th NUH-‐NUS Annual Scientific Meeting, NUS, Singapore. August 2002.

Lu H-‐F, WS Lim, P-‐C Zhang, SM Chia, H Yu, H-‐Q Mao, KW Leong. Galactosylated PVDF Hollow Fibers for Primary Hepatocyte Culture. 6th NUH-‐NUS Annual Scientific Meeting, NUS, Singapore, August 2002.

Lu H-‐F, WS Lim, P-‐C Zhang, KW Leong, H-‐Q Mao. Co-‐culture of Rat Hepatocyte Spheroids with 3T3 Cells Produced High Synthetic Function. 17th European Conference on Biomaterials, Barcelona, Spain, September 2002.

Leong KW, Lu HF, Yin C, Yang JC, Chua K, Chen F, Ramakrishna S, Mao HQ. Functional fibrous scaffolds. 5th International Tissue Engineering Conference, Kobe, Japan, December 2002

Chan V, Mao HQ, Liao, K, Leong KW. Adhesion contact dynamics of HEPG2 cells on biomimetic substrates. 5th International Tissue Engineering Conference, Kobe, Japan, December 2002

Choi JH, Park DJ, Sun D, Wan A, Sieber A, Kostuik J, Leong KW. Synthetic hydrogels formed in situ to replace the nucleus pulposus. 5th International Tissue Engineering Conference, Kobe, Japan, December 2002

Park DJ, Song RJ, Choi JH, Wen J, Leong KW. Combined gene and cell delivery for tissue engineering. 5th International Tissue Engineering Conference, Kobe, Japan, December 2002

Wan A, Lim E, Leong KW. Self-‐assembled fibers for tissue engineering. 5th International Tissue Engineering Conference, Kobe, Japan, December 2002

Petersen E, LeVisage C, Lum L, Elisseeff J, Leong KW. Mesenchymal differentiation of human stem cells. 5th International Tissue Engineering Conference, Kobe, Japan, December 2002

Mao HQ, Yin C, Ying L, Zhuo RX, Kang ET, Leong KW. Effect of substrate configuration on hepatocyte morphology and functional maintenance. 5th International Tissue Engineering Conference, Kobe, Japan, December 2002

LeVisage C, Kim S, Tateno K, Sieber A, Kostuik J, Leong KW. Interaction of human mesenchymal stem cells with adult degenerative disc cells. 17th Annual Meeting of North American Spine Society, Quebec, Canada, November, 2002.

Bursac N., Loo, Y., Irby, M.E., Leong, KW., Tung, L. “Polymer Scaffolds for Anisotropic Growth of Engineered Cardiac Tissue”, Southern BMES conference, Washington DC, 2002.

Wen J, Leong KW. Synthesis and characterization of poly(e-‐caprolactone-‐co-‐ethyl ethylene phosphate) for tissue engineering. The 29th International Symposium on Controlled Release of Bioactive Materials, Seoul, Korea, July 2002.

Chew, JL, Fu, TQH, Mao, HQ, Leong, KW and Chua, KY (2002). "Oral Administration of Major House Dust Mite Allergen Genes Complexed with Chitosan Elicits Protective Th1-‐Skewed Immunity in Mice." Allergy 57: 64-‐64.

Du, X, Le Visage, C, Chen, HH, Leong, KW, Hamper, UM and Yang, X (2002). "Ultrasound Imaging of Catheter-‐Based Vascular Gene Delivery: An in Vivo Feasibility Study." Radiology 225: 251-‐251.

Du, XY, Yang, YS, Le Visage, C, Chen, HH, DeJong, R, Wang, DM, Leong, KW, Hamper, UM and Yang, XM (2002). "In Vivo Ultrasound Imaging of Catheter-‐Based Vascular Gene/Microsphere Delivery." Circulation 106(19): 332-‐332.


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Dai, H, Zhang, PC, Lim, WS, Chen, Y, Leong, KW and Mao, HQ (2003). "Liver-‐Targeted Gene Transfer Mediated by Chitosan-‐DNA Nanoparticles through Bile Duct, Portal Vein and Tail Vein Injections." Mol Ther 7(5): S322-‐S323.

Lee, IL, Wang, J, Lim, WS, Chia, SM, Yu, H, Leong, KW and Mao, HQ (2003). "Collagen-‐Based Non-‐Viral Gene Carriers." Mol Ther 7(5): S222-‐S222.

Li, XH, Bright, C, Park, YS, Tateno, K, Sieber, A, Kostuik, JP and Leong, KW (2003). "Non-‐Viral Gene Transfer for Spine Fusion." Mol Ther 7(5): S377-‐S378.

Song, RJ and Leong, KW (2003). "The Effects of Mip-‐1 Alpha, Mip-‐3 Alpha, and Mip-‐3 Beta on the Induction of Gag-‐Specific Immunity with DNA Vaccines." Mol Ther 7(5): S257-‐S257.

Tsurushima, H, Yuan, X, Dillehay, L and Leong, KW (2003). "Radio-‐Responsive Gene Transfection of Malignant Glioma Cells." Mol Ther 7(5): S415-‐S416.

Wong CW, Miller LD, Zhang PC, Chua KCN, Liu ET, Leong KW, Mao HQ. Gene expression profiling of hepatocytes grown on different substrates: Identification of candidate genes important for maintaining hepatic function of hepatocytes in culture. HUGO Human Genome Meeting, Cancún, Mexico, April 2003.

Lu HF, Zhang PC, Lim WS, Leong KW, Mao HQ. Coculture of hepatocyte spheroids with human mesenchymal stem cells. The 2003 Annual Meeting and Exposition, Society for Biomaterials, Reno, NV, April 2003

Wang XL, Zhang PC, Dai H, Leong KW, Mao HQ. Effect of pendant chain length of polyphosphoramidate gene Carriers on their transfection efficiency. The 2003 Annual Meeting and Exposition, Society for Biomaterials, Reno, NV, April 2003

Wang XL, Zhang XQ, Zhang PC, Leong KW, Mao HQ. Liver-‐targeted polyphosphoramidate gene carriers. The 30th International Symposium on Controlled Release of Bioactive Materials, Glasgow, UK, July 2003

Dai H, Tan G, Zhang PC, Lim WS, Chen Y, Leong KW, Mao HQ. Polymer-‐DNA nanoparticles for liver targeted gene delivery. International Conference on Materials for Advanced Technologies, Singapore, December 2003

Chua KN, Lim WS, Chai C, Wen J, Ramakrishna S, Leong KW, Mao HQ. Galactose ligand-‐conjugated nanofiber scaffold for hepatocyte culture. International Conference on Materials for Advanced Technologies, Singapore, December 2003

Lim WS, Lu HF, Zhang PC, Leong KW, Mao HQ. Transfection of rat primary hepatocyte spheroids and hepatocyte monolayer using a novel polyphosphoramidate gene carrier. International Conference on Materials for Advanced Technologies, Singapore, December 2003

Chia SM, Lin PC, Quek CH, Leong KW, Yu H, "TGF-‐ß1 Regulation in Hepatocyte-‐NIH3T3 Co-‐culture is Important for the Enhanced Hepatocyte Functions," Tissue Engineering Society International (TESI) Annual Meeting, Orlando, Florida, USA, Dec 10-‐13, 2003.

Lu HF, Lim WS, Zhang PC, Leong KW, Mao HQ. Coculture of hepatocyte spheroids with human mesenchymal stem cells. International Conference on Materials for Advanced Technologies, Singapore, December 2003

Wang XL, Zhang XQ, Zhang PC, Wang J, Leong KW, Mao HQ. Novel cationic polyphosphoramidates as gene carriers: Structure-‐function relationship. International Conference on Materials for Advanced Technologies, Singapore, December 2003

Zhang PC, Wang XL, Wang J, Mao HQ, Leong KW. Intracellular trafficking of DNA mediated by polyphosphoramidate gene carriers. International Conference on Materials for Advanced Technologies, Singapore, December 2003

Zhang XQ, Wang XL, Zhuo RX, Liu ZL, Leong KW, Mao HQ. “Efficient gene transfection using PAMAM dendrimer with trimesic acid core.” International Conference on Materials for Advanced Technologies, Singapore, December 2003

Zhang XQ, Wang XL, Zhang PC, Zhuo RX, Leong KW, Mao HQ. Galactosylated polyphosphoramidates for liver-‐targeted gene delivery. International Conference on Materials for Advanced Technologies, Singapore, December 2003

LeVisage C, Yang S-‐H, Tateno K, Sieber A, Kostuik J, Leong KW. “Small Intestinal Submucosa (SIS) as a Potential Bioscaffold for Intervertebral Disc Regeneration,” Proceedings of the 6th Annual Conference of the Tissue Engineering Society International (TESI), Orlando, USA, December 2003


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Chia SM, Lin PC, Quek CH, Leong KW, Yu H. “TGF-‐β1 Regulation in Hepatocyte-‐NIH3T3 Co-‐culture is Important for the Enhanced Hepatocyte Functions.” Tissue Engineering Society International (TESI) Annual Meeting, Orlando, Florida, USA, Dec 10-‐13, 2003

LeVisage C, Dunham B, Flint P, Leong KW. "Towards Tissue Engineering of Trachea.” Proceedings of the 1st International Conference On Epithelial Technologies and Tissue Engineering (ICETTE), Singapore, December 2003

Dang JY, LeVisage C, Sieber A, Kostuik, Leong KW. “Thermosensitive Chitotsan Gels for use as an Injectable Tissue Therapy Device.” Tissue Engineering Society International Conference, Orlando, December, 2003.

Yang, S-‐H, LeVisage C, Kim S, Tateno K, Sieber A, Kostuik J, Leong KW. “Small intestinal submucosa (SIS) as a potential bioscaffold for intervertebral disc regeneration.” Proceedings of the Biomedical Engineering Society Annual Fall Meeting, Nashville, USA, October 2003

LeVisage C, Kim S, Tateno K, Sieber A, Kostuik J, Leong KW. “Interaction of Human Mesenchymal Stem Cells with Disc Cells: Changes in Biosynthesis of Extracellular Matrix.” Proceedings of the Biomedical Engineering Society Annual Fall Meeting, Nashville, USA, October 2003

LeVisage C, Tateno K, Sieber A, Kostuik J, Leong KW. “Interaction of Human Mesenchymal Stem Cells with Disc Cells: Changes in Biosynthesis of Extracellular Matrix,” Proceedings of the 30th Annual Meeting of the International Society for the Study of the Lumbar Spine (ISSLS), Vancouver, Canada, May 2003

LeVisage C, Dunham B, Flint P, Leong KW. “Co-‐culture of Mesenchymal Stem Cells and Respiratory Epithelial Cells to Engineer a Human Tracheal Mucosa,” Transactions of the 29th Society for Biomaterials Annual Meeting, Vol. 16, Reno, Nevada, USA, May 2003

LeVisage C, Dunham B, Flint P, Leong KW. “Biological and Polymeric Substrates to Engineer a Respiratory Neomucosa.” Proceedings of the 7th Annual Hilton Head Worshop on Engineering Tissues, Hilton Head, USA, March 2003

Sun DN, Wang J, Li XH, Tateno K, Sieber A, Kostuik JP, Leong KW, "Characterizing the Local Properties of Annulus Fibrosus by Indentation.” Transactions of Biomedical Engineering Society, 2003.

Li Q, Wang J, Sun DN, Elisseeff J, Leong KW, "Biodegradable Photocrosslinked Hydrogel Based on Polyphosphoester.” Transactions of Biomedical Engineering Society, 2003.

Bowman K, Sarkar R, Tetreault R, Leong KW, “Non-‐viral Gene Therapy for Hemophilia A Through Oral Delivery of Chitosan Nanoparticles,” The 31st International Symposium on Controlled Release of Bioactive Materials, Hawaii, June 2004

Lim S, Liao IC, Leong KW, “Gene Delivery from Nonwoven Fibrous Scaffolds,” The 31st International Symposium on Controlled Release of Bioactive Materials, Hawaii, June 2004

Sun DN, Wang J, Li XH, Tateno K, Sieber A, Kostuik JP, Leong KW, “Indentation Reveals the Composition-‐Property Relationship of Annulus Fibrosus,” Annual Conferences of Orthopaedic Research Society, 2004.

Dang JY, LeVisage C, Sieber A, Kostuik, Leong KW. “Thermosensitive Chitosan Gel Based Therapeutic for the Treatment of Degenerative Disc Disease,” Biomedical Engineering Society Annual Fall Meeting, Philadelphia, October 2004.

Dang JY, LeVisage C, Sieber A, Kostuik, Leong KW. “Thermosensitive Chitosan Gels for Use as an Injectable Tissue Therapy Device” Gordon Research Conference: Signal Transduction by Engineered Extracellular Matrices, Lewiston, 2004.

Chua KN, Ramakrishna S, Leong KW, Mao HQ. “Stable immobilization of rat hepatocyte spheroids on galactosylated nanofiber scaffold,” The 17th Polymer Networks group Meeting, Bethesda, MD. August 2004

Chua KN, Lim WS, Lu HF, Ramakrishna S, Leong KW, Mao HQ. “Functional fibrous scaffolds for liver tissue engineering,” The 2004 Materials Research Society Fall Meeting, Boston, MA. Nov. 2004

Leong, KW, Chien, I, Lim, E, Lim, S, Wan A, Wen, J, Chiew, SY, Cai J, Ramakrishna, S, Mao, HQ. Controlled release systems designed for regenerative medicine. The 7th World Biomaterials Congress, Sydney, Australia, May 2004.

Chua KN, Lim WS, Lu HF, Chai C, Zhang PC, Wen J, Ramakrishna S, Leong KW, Mao HQ. Immobilized hepatocyte spheroids on galactose ligand-‐conjugated nanofiber scaffold. The 7th World Biomaterials Congress, Sydney, Australia, May 2004.


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Lim WS, Chua KN, Wang XL, Zhang PC, Lu HF, Leong KW, Mao HQ. Transfection of rat primary hepatocytes using a novel polyphosphoramidate gene carrier. The 7th World Biomaterials Congress, Sydney, Australia, May 2004.

Chai C, Feng Q, Jiang, XS, Leong KW and Mao HQ. Bone marrow CD34+ cell expansion on polyethylene terephthalate (PET) scaffold conjugated with hematopoietic growth factors. The 7th World Biomaterials Congress, Sydney, Australia, May 2004.

Feng Q, Jiang XS, Chai C, Lim WS, Leong KW, Mao HQ. Ex vivo expansion of hematopoietic primitive cells on ECM molecule immobilized scaffolds. The 7th World Biomaterials Congress, Sydney, Australia, May 2004

Bowman, K., R. Sarkar, X. L. Wang, H. Q. Mao and K. W. Leong (2004). “Evaluation of non-‐viral vectors for gene therapy of hemophilia A.” Mol Ther 9: S314-‐S314.

Dai H, Jiang X, Lim WS, Jiang XS, Chen Y, Mao HQ, Leong KW. (2004). “Selective depletion of Kupffer cells leads to enhanced transgene expression in rat liver following intraductal infusion of naked DNA and DNA nanoparticles.” Mol Ther 9: S136-‐S137.

Zhang Y, Teoh SH, Chai C, and KW Leong, “Fibronectin immobilized by covalent conjugation or physical adsorption shows different bioactivity on PET.” 3rd International Conference on Materials Applied Technology, Singapore, July, 2005

Zhang Y, Teoh SH, Chai C, and KW Leong, “Hematopoietic stem cell co-‐expansion with mesenchymal stem cells under different co-‐cultivation systems,” 3rd International Conference on Materials Applied Technology, Singapore, July, 2005

Li J, Li X, Li XP, Tan NK, The HH, Lim CT, Ramakrishna S, Leong KW. “Self-‐assembled nanostructures formed by amphiphilic block copolymer.” 3rd International Conference on Materials Applied Technology, Singapore, July, 2005

Pan WX, Petersen E, Cai N, Liu K, Leong KW. “Viscoelastic properties of human mesenchymal stem cells,” J Biotechnology 118: S67-‐S67 Suppl. 1 Aug, 2005

Xu YA, Low HY, Yim EKF, Chen S, Leong KW. “Fabrication of nanopatterns by nanoimprinting and their influence on cellular behavior.” 3rd International Conference on Materials Applied Technology, Singapore, July, 2005

Ye L, Wu D, Wong K, Sun G, He CB, Leong KW. “Novel biodegradable polymer for safe and efficient delivery of DNA,” 3rd International Conference on Materials Applied Technology, Singapore, July, 2005

Mao HQ, Leong KW. “Polymeric nanoparticles for nonviral gene delivery,” 3rd International Conference on Materials Applied Technology, Singapore, July, 2005

Chew SY, Yim EKG, Leong KW. “Controlled Release Micro/Nano-‐ordered Structures for Tissue Engineering.” Proceedings of International Conference on Controlled Release of Bioactive Agents, Miami, June 2005

Yoo HS, Wang LF, Chen HH, Leong KW. “Poly(ethylenimine)-‐poly(ethylene glycol) grafted chitosan for non-‐viral gene delivery.” Proceedings of International Conference on Controlled Release of Bioactive Agents, Miami, June 2005

Bowman K, Du X, LeVisage C, Sarker R, Yang XM, Leong KW. “Factor VIII Gene Expression after Catheter-‐Based DNA Nanoparticle Delivery.” Proceedings of International Conference on Controlled Release of Bioactive Agents, Miami, June 2005

Bowman K, Sarker R, Wang X, Mao HQ, Raut S, Leong KW. “Evaluation of polymer-‐DNA nanoparticles for FVIII gene delivery.” Proceedings of International Conference on Controlled Release of Bioactive Agents, Miami, June 2005

Yim EFK, Leong KW, “Neuronal differentiation of human mesenchymal stem cells induced by nanotopography.” Annual Fall Meeting of Biomedical Engineering Society, Baltimore, 2005

Chen H, Ho M, Wang J, Leong KW, “QD-‐FRET for investigation of dissociation and intracellular trafficking of DNA nanoparticles.” Annual Fall Meeting of Biomedical Engineering Society, Baltimore, 2005

Dang JY, Sieber A, Kostuik J, Leong KW. “Differential response to direct & indirect interactions between mesenchymal stem cells & disc cells.” Annual Fall Meeting of Biomedical Engineering Society, Baltimore, 2005

Dang JY, Chew SY, Leong KW, “Cellular response to thermoreversible chitosan matrices containing micro-‐/nano-‐scaled topographies.” Annual Fall Meeting of Biomedical Engineering Society, Baltimore, MD 2005


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Bowman K, Sarkar R, Raut S, Shah N, Wang XD, Mao HQ, Leong KW. “Oral delivery of non-‐viral DNA nanoparticles for hemophilia A.” American Society of Gene Therapy, 8th Annual Meeting. June 2005. St. Louis, MO.

Campbell CT, LeVisage C, Sun DDN, Dang JY, Kebaish K, Serhan H, Sieber AN, Kostuik JP, Leong KW. “Biomechanical analysis of lumbar discs following nuclear replacement in a non-‐human primate model.” Annual Fall Meeting of Biomedical Engineering Society, Baltimore, MD 2005

Kadakia LV, LeVisage C, Sieber AN, Kostuik JP, Leong KW. “Culture of human mesenchymal stem cells with SIS in pellet configuration.” Annual Fall Meeting of Biomedical Engineering Society, Baltimore, MD 2005

Bakhru S, Ong S, Link TW, Leong KW, Mao HQ. “Controllable fabrication of polymeric microcapsules for neural stem cell culture.” Annual Fall Meeting of Biomedical Engineering Society, Baltimore, MD 2005

Klausner E, Bachelor E, Matzinger P, Leong KW. “Oral delivery of insulin plasmid using chitosan nanoparticles.” 3rd International Nanomedicine and Drug Delivery Symposium, Baltimore, MD 2005

Haider M, Ghandehari H, Leong KW. “Genetically engineered silk-‐elastinlike hydrogels for the culture of human mesenchymal stem cells.” 3rd International Nanomedicine and Drug Delivery Symposium, Baltimore, MD 2005

Quek CH,Chua KN, Mao HQ, Leong KW. “Interfacial polyelectrolyte complexation synthesis of fibrous scaffolds for rat hepatocyte culture.” The 12th International Conference on Biomedical Engineering, Singapore, Dec 7-‐10, 2005.

Guan J, Fei ZZ, Loo Y, Leong KW, Lee J. “Dynamic Self-‐Assembly and Characterization of DNA-‐Polycation Nanoparticles.” American Institute of Chemical Engineering Annual Meeting, Cincinnati, OH, 2005.

Yim EFK, Hu W, Pang S, Leong KW. “Significance of nanotopographical cues in directing differentiation of adult stem cells.” International Tissue Engineering Society Meeting, Shanghai, China, 2005

Chew SY, Rui R, Hooke A, Leong KW, “Synergistic effect of aligned fibers and sustained release of GDNF on sciatic nerve regeneration,” International Tissue Engineering Society Meeting, Shanghai, China, 2005

Dang, JY, Sieber A, Kostuik J, Leong KW. “Investigation of Hydroxybutyl Chitosan as an Injectable Cell Delivery System for Nucleus Pulposus Replacement,” Orthopaedic Research Society 51st Annual Meeting, Washington, February 2005.

Jiang X, Dai H, Leong KW, Mao HQ. “PEG-‐b-‐ppa/DNA micelles for liver-‐targeted gene delivery,” in 31st Annual Meeting of the Society for Biomaterials, Pittsburgh PA 2006.

Yim EFK, Leong KW. “Response of human embryonic and adult mesenchymal stem cells to nanotopography,” in 31st Annual Meeting of the Society for Biomaterials, Pittsburgh PA 2006.

Chua KY, Tang YN, Lee PC, Leong KW, Ramakrishna S, Mao HQ. “Fabrication of dual-‐functional electrospun nanofiber scaffolds for liver tissue engineering,” in 31st Annual Meeting of the Society for Biomaterials, Pittsburgh PA 2006.

Chua KY, Chai C, Jiang XS, Leong KW, Ramakrishna S, Mao HQ. “Surface-‐aminated electrospun poly(ethersulfone) fibers enhances ex vivo expansion of human umbilical cord blood hematopoietic progenitor (cd34+) cells. in 31st Annual Meeting of the Society for Biomaterials, Pittsburgh PA 2006.

Dang JM, Chew SY, Leong KW. “Thermally responsive chitosan as an injectable matrix for therapeutic delivery of mesenchymal stem cells,” in 31st Annual Meeting of the Society for Biomaterials, Pittsburgh PA 2006.

Dang JM, Chew SY, Leong KW. “Topography Induced Stem Cell Differentiation and Aligned Cell Sheets Using Thermosensitive Nanofibers,” Biomedical Engineering Society Annual Fall Meeting, Chicago, October 2006.

Chen H, Ho M, Wang J, Leong KW. “Quantitative Model of Intracellular Trafficking Constructed from Quantum Dot-‐FRET,” Annual Meeting, Biomedical Engineering Society, Chicago, October, 2006.

Chen H, Ho M, Wang J, Leong KW. “Intracellular Trafficking of QD-‐Fret Nanoparticles for Gene Delivery,” Annual Meeting, American society of Gene Therapy, Baltimore, May, 2006.

Liao IC, Chew SY, Leong KW. “Controlled Release of Bioactive Protein from Aligned core-‐Shell Nanofibers,” 33rd Annual Meeting of Controlled Release Society, Vienna, Austria, July, 2006.

Liao IC, You LC, Leong KW. “Micro/Nano Fibers Embedded with Live Cell and viral Vectors,” Biomedical Engineering Society Annual Meeting, 2006, Chicago, October 11-‐14, 2006.

Chen S, Xu Y, Yim EKF, Low HY, Leong KW. “Microphage Behavior on Micron-‐and Sub-‐Micron Sized Topography,” Biomedical Engineering Society Conference, Chicago, October, 2006.


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Liao IC, Liu J, Chew SY, Leong KW. “Localized viral gene delivery through co-‐axial electrospun fibers” American Society of Gene Therapy, Baltimore, May, 2007.

Yow SZ, Quek CH, Chai C, Lim CT, Leong KW. “Polyelectrolyte-‐Complexation Fibers For 3D Cell Patterning And Regenerative Medicine,” Annual Meeting of Biomedical Engineering Society, Los Angeles, October, 2007.

Chen H, Ho YP, Jiang X, Mao HQ, Wang J, Leong KW. “Quantitative analysis of intracellular unpacking of polymeric DNA nanoparticles constructed from quantum dot-‐FRET,” Annual Meeting of American Society for Gene Therapy, Seattle, June, 2007.

Chen H, Ho YP, Wang JH, Leong KW. “Evaluating the release and integrity of DNA from nanocomplexes for gene delivery by two-‐step quantum dot-‐FRET,” Annual Meeting of Biomedical Engineering Society, Los Angeles, October, 2007.

Chen H, Ho YP, Wang JH, Leong KW. “Evaluating the release and integrity of DNA from nanocomplexes for gene delivery by two-‐step quantum dot-‐FRET,” Duke Fitzpatrick Institute for Photonics, Durham, October, 2007.

Loo Y, Leong KW. “Elucidating the fate of polymer-‐DNA nanoparticles in oral gene delivery,” Cold Spring Harbor Conference, November, 2007.

Liao IC, Liu J, Bursac N, Leong KW. “Synchronized electrical and mechanical stimulation improves myotube maturation on aligned nanofibers” 16th International Conference of Mechanics in Medicine and Biology, Pittsburgh, PA, 2008.

Kulangara K, Yang Y, Leong KW. “Response of human Mesenchymal Stem Cells to Nanotopograohy”, Gordon Research Conference, Signal Transduction by Engineered Extracellular Matrices, Lewiston, ME. July, 2008.

Loo Y, Yamanaka YJ, Leong KW. “Biodistribution of quantum-‐dot labeled DNA nanoparticles in intestinal constructs” Annual Meeting of American Society for Gene Therapy, Seattle, June, 2008.

Loo Y, Yamanaka YJ, Leong KW. “Biodistribution of quantum-‐dot labeled DNA nanoparticles in intestinal constructs” Fitzpatrick Institute of Photonics Meeting 2008.

Christoforou N, Oskouei BN, Hill CM, Bian W, Bursac N, Leong KW, Hare JM, Gearhart JD. “Engraftment, differentiation and functional improvement of cardiac output following transplantation of mouse embryonic stem cell-‐derived cardiac progenitor cells in the infarcted myocardium” International Society for Stem Cell Research, Philadelphia PA, June 2008.

Christoforou N, Chakraborty S, Leong KW. “Bioengineering a cardiac tissue patch by utilizing a combination of embryonic stem cell derived cardiac progenitors and a fibrin gel matrix on a sheet of electrospun aligned nanofibers” International Society for Stem Cell Research, Philadelphia PA, June 2008.

Liao IC, Liu J, Bursac N, Leong KW. “Effect of electromechanical stimulation on the maturation of myotubes on aligned electrospun fibers” Annual Meeting, Biomedical Engineering Society, St. Louis, MO, October 2008.

Christoforou N, Chackraborty S, Leong KW, “Engineering a cardiac patch from cardiac progenitors and nanofibrous scaffolds” Annual Meeting, Biomedical Engineering Society, St. Louis, MO, October 2008.

Yang Y, Kulangara K, Sia J, Leong KW. “Engineering microfluidic platform with nanoscale features for stem cell study” Annual Meeting, Biomedical Engineering Society, St. Louis, MO, October 2008.

Yang Y, K. Kulangara K, Leong KW. “Engineering microfluidic platform with nanoscale features for stem cell study” Abstract presentation. American Chemical Society National Meeting, Salt Lake City, UT, March 2009.

Li H, LaBean TH, Leong KW. Self-‐assembled DNA nanostructures: novel carriers for nucleic acid drug delivery. BMES Annual Fall Meeting, Pittsburgh, PA, 2009.

Ho YP, Chen HH, Leong KW, Wang TH. “Spatially-‐resolved analysis of DNA nanocomplex self-‐assembly enabled by integrating nanophotonics and microfluidics” Biophysical Society 53rd Annual Meeting & 17th International Biophysics Congress, Boston, MA, 2009.

Ho YP, Duan Y, Zhao F, Leong KW. “Integration of nanophotonics and microfluidics: controlled synthesis and real-‐time characterization of DNA nanocomplexes for gene delivery” 9th Fitzpatrick Institute for Photonics Annual Meeting on Frontiers in Photonics Science and Technology. Durham, NC, 2009.

Ho YP, Chen HH, Leong KW, Wang TH. “Spatially-‐resolved analysis of DNA nanocomplex self-‐assembly enabled by integrating nanophotonics and microfluidics” Biophysical Society 53rd Annual Meeting & 17th International Biophysics Congress, Boston, MA, 2009.


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Yang Y, Leong KW. “Engineering Multifunctional Microfluidic Platform for Stem Cell Study” AIChE Annual Meeting, Nashville, TN, 2009.

Yang Y, Kulangara K, Lam R, Leong KW. “Engineering Multifunctional Microfluidic Platform for Stem Cell Study” BMES Annual Meeting, Pittsburgh, PA, 2009.

Zhao F, Duan YJ, and Leong KW. “Completely Biological Tissue-‐engineered Small-‐diameter Blood Vessel from Aligned Human Mesenchymal Stem Cells.” NHLBI Symposium on Cardiovascular Regenerative Medicine. Bethesda, MD, 2009.

Zhao F, Velhuis JJ, Duan YJ, and Leong KW. “Maintaining stemness of human mesenchymal stem cells on synthetic nanostructures.” Gordon Research Conference: Biomaterials: Biocompatibility/Tissue Engineering, Holderness, NH, 2009.

Zhao F and Leong KW. “Small diameter blood vessel regeneration by biomimetic engineering.” Flight Attendant Medical Research Institute’s Eighth Scientific Symposium. Boston, MA, 2009.

Andrew F. Adler, Alessondra T. Speidel, Kristian Kolind, Morten Foss, Kam W. Leong, Modulation of transfectability with substrate topography: emerging links to endocytosis, BMES Annual Meeting, Austin, TX, October 2010

Grigsby C, Ho M, Leong KW. Functional Performance of Polyplexes Self-‐ Assembled in Microfluidics–Generated Droplets, BMES -‐ Austin, TX -‐ Octoboer 2010

C. H. Quek, J. Fang, K. W. Leong, Synthesis of transition-‐metal doped zinc selenide quantum dots for bioimaging, BMES, Austin, Texas, USA, Oct 6-‐9, 2010.

Zhao F, Jiang Y, Chou A, and Leong KW. Cell sheet engineering of small-‐diameter blood vessels by aligned human mesenchymal stem cells under physiologically-‐relevant conditions. TERMIS-‐NA 2010 Annual Conference. Dec 5-‐8, 2010, Orlando, FL.

Zhao F, Jiang Y, Chou A, and Leong KW. Engineering Scaffold-‐free Vascular Grafts by Aligned Human Mesenchymal Stem Cells. NCTERMS 2010. Nov 12, 2010, Durham, NC.

Zhao F and Leong KW. Small diameter blood vessel regeneration by biomimetic engineering. Flight Attendant Medical Research Institute’s Eighth Scientific Symposium. May 3-‐5, 2010, Miami, FL.

Zhao F and Leong KW. Cell Sheet Engineering of Small-‐diameter Blood Vessel from Aligned Human Mesenchymal Stem Cells. Southern Biomedical Engineering Conference. Apr 30-‐May 2, 2010, Baltimore, MD.

Y. Yang, R. Lam, K. Kulangara and K.W. Leong, “Surface Modification of Polymeric Substrate: Chemical, Topographical and Mechanical Cues Influencing Stem Cell Behavior”, AIChE Annual Meeting, paper 83c, Salt Lake City, UT (November 2010)

Y. Yang, R. Dharmawan, K. Kulangara and K.W. Leong, “Mechanical Property Change Induced by Chemical Modification of PDMS Influences Stem Cell Behavior”, AIChE Annual Meeting, paper 178e, Salt Lake City, UT (November 2010)

Y. Yang, R. Dharmawan, K. Kulangara, R. Lam and K.W. Leong, “Coupling of Chemical and Mechanical Changes Induced by Surface Modification of PDMS Influences Stem Cell Behavior”, Gordon Conference: Signal Transduction by Engineered Extracellular Matrices, Biddeford, ME (June-‐July 2010)

Y. P. Ho, C. Grigsby, F. Zhao, H. Li, K. W. Leong, "DNA Nanocomplexes Synthesized in a Microfluidic Droplet Generator," The Biomedical Engineering Society Annual Meeting (BMES), Austin, USA, 2010.

H. Li, Y. P. Ho, T. LaBean, K. W. Leong, "Self-‐assembling DNA Nanostructures: Novel Carriers for Drug Delivery," The Biomedical Engineering Society Annual Meeting (BMES), Austin, USA, 2010.

Y. Wu, Y. Mao, Y. P. Ho, X. Wang, B. Yu, K. W. Leong, L. J. Lee, "The Study and Comparison of the Uptaking and Intracellular Trafficking of Lipoplex Nanoparticles and Polyplex Nanoparticles by Quantum Dots-‐FRET,” American Institute of Chemical Engineers Annual Meeting, Salt Lake City, USA, 2010.

Y. P. Ho, Y. Duan, F. Zhao, K. W. Leong, "Controlled Synthesis of DNA Nanocomplexes In A Microfluidic Device," Biophysical Society 54th Annual Meeting & 18th International Biophysics Congress, San Francisco, USA, 2010.

Liao, I.C. and Leong, K.W. “Integrative skeletal muscle tissue engineering for hemophilia A”. Gordon Research Conference – Musculoskeletal Biology and Bioengineering. Proctor Academy, NH, 2010.


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Karina Kulangara, Yong Yang, Jennifer Yang and Kam W. Leong. Substrate Topography Modulates Stem Cell Function. Gordon Conference. Signal Transduction by Engineered Extracellular Matrices, Biddeford, ME, June 27-‐July 2. 2010

M. Ho, Y.Yang, K. Kulangara, H. Li, F. Zhao, T. LaBean and K.W. Leong. Microfluidics Platforms Related to Nanomedicine. Foundation of Nanoscience (FNANO). Snowbird, Utah, April 27-‐30. 2010

Karina Kulangara, Yong Yang and Kam W. Leong, Response of human Mesenchymal Stem Cells to Nanotopography in Artificial Niches. Keystone Conference. Stem Cell Differentiation and Dedifferentiation, Keystone, CO, February 15-‐20. 2010

Syandan Chakraborty, Nicolas Christoforou, Kam Leong. 2010 Biomedical Engineering Society Annual Meeting held in Austin, Texas on October 6-‐9, 2010.

Christoforou N, Liau B, Leong K, Bursac N, Engineering a Highly Functional 3D Cardiac Tissue Patch from Embryonic Stem Cell-‐derived Cardiogenic Cells, Cardiac Electrophysiological Society, Chicago IL, November 2010

Chakraborty S, Christoforou N, Leong K, Derivation of Cardiovascular Progenitors From Pluripotent Stem Cells, Biomedical Engineering Society, Austin TX, October 2010.

Liau B, Christoforou N, Leong K, Bursac N, Multipotent mESC-‐Derived Cardiovascular Progenitors, But Not Cardiomyocytes Generate Highly Functional 3-‐D Engineered Cardiac Tissues, Birth Life and Death of Cardiac Myocyte, Napa Valley CA, June 2010.

Christoforou N, Chellappan M, Wu T, Chakraborty S, Adler A, Leong KW, Engineering a Cardiac Tissue Patch With Cardiac Progenitor Cells, Flight Attendant Medical Research Institute Annual Meeting, Miami FL, May 2010.

Christoforou N, Chellappan M, Chackraborty S, Wu T, Leong KW, Genetically Engineering the Transdifferentiation of Mouse and Human Primary Cells Towards the Cardiac Cell Lineage, Keystone Symposia: Stem Cell Differentiation and Dedifferentiation, Keystone CO, March 20

Phua K, Leong KW. Enhancing transfection efficiencies of mRNA Lipopolyplexes on JAWS II cells. BMES Annual Meeting, Austin, TX, October 2010

Le DM, Kulangara K, Leong KW, Sheares-‐Ashby V. Shape memory polymers in dictating cellular response. Abstracts of Papers of the American Chemical Society, 241. (2011)

Sheares-‐Ashby V, Le D, Rochette J, White S, Kulangara K, Leong KW. Shape memory biomaterials: Variation and study of functionality, stimuli and topography. Abstracts of Papers of the American Chemical Society, 241. (2011)

White SM, Brown A, Leong KW, Sheares-‐Ashby V. Functional shape memory polymers tailored for cellular response applications. Abstracts of Papers of the American Chemical Society, 241. (2011)

Lee EJ, Jing L, Christoforou N, Mou Y, Leong KW, Setton LA, Chen J. Direct differentiation of mouse induced pluripotent stem cells into nucleus pulposus-‐like cells. Annals of Biomedical Engineering 39, (2011)

Adler A, Speidel A, Christoforou N, Kolind K, Leong KW. Enhancement of Transfection Efficiency with Substrate Topography: Reverse Transfection and siRNA. in BMES Annual Meeting. 2011. Hartford CT.

Grigsby C, Ho Y, Lin C, Engbersen J, Leong KW. Self-‐assembly of bioreducible nucleic acid nanocomplexes with microfluidics. in BMES Annual Meeting. 2011. Hartford CT.

Grigsby CL, Ho YP, Leong KW. Synthesis of poly(amido amine)-‐nucleic acid nanocomplexes with microfluidics. in North Carolina ACS Annual Meeting. 2011. Raleigh NC.

Grigsby CL, Ho YP, Leong KW. Properties of polymer-‐nucleic acid nanocomplexes synthesized with microfluidics-‐assisted confinement. in Triangle Soft Matter Workshop. 2011. Chapel Hill.

Grigsby CL, Ho YP, Leong KW. Reduced Toxicity of Nanocomplexes Synthesized with Microfluidics-‐Assisted Confinement. in North Carolina Society of Toxicology Meeting. 2011. Research Triangle Park NC.

Ho YP, Grigsby CL, Leong KW. Unexpected properties of polymeric DNA nanocomplexes synthesized in picoliter droplets. in 6th Annual IEEE-‐NEMS Meeting. 2011. Kaohsiung, Taiwan.

Ho YP, Grigsby CL, Leong KW. Microfluidics-‐Assisted Synthesis of Gene-‐Loaded Nanocomplexes. in Physics & Chemistry of Microfluidics, Gordon Research Conference. 2011. Waterville Valley, NH.


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Juul S, Ho YP, Stougaard M, Koch J, Andersen FF, Leong KW, Knudsen BR. Microfluidics-‐Mediated Isothermal Detection of Enzyme Activity at the Single Molecule Level. in 33rd Annual International Conference of the IEEE Engineering in Medicine and Biology Society. 2011. Boston, MA.

Kulangara K, Yang Y, Yang J, Leong KW. Substrate Topography Modulates Stem Cell Function. in Keystone Symposium Stem Cells in Development, Tissue Homeostasis and Disease. 2011. Santa Fe, NM.

Kulangara K, Leong KW. Nanotopography Modulates Mesenchymal Stem Cell Function and Neural Transdifferentiation. in Southeastern Regional Meeting of the American Chemical Society 2011. Richmond, VA.

Zhao F, Jiang Y, Chou A, Ren L, Chen L, Klitzman B, Leong KW. In-‐vivo Remodeling of Scaffold-‐free Small-‐Diameter Blood Vessels with Aligned Human Mesenchymal Stem Cells. in TERMIS-‐NA 2011 Annual Conference. 2011. Houston, TX.

Patents: Issued patent applications: Wozniak, J. and Leong, K., "Non-‐reuseable vaccine injection system” U.S.A. 4,781,683, 1989 Rosen, H., Linhardt, R., Leong, K., and Langer, R., "Bioerodible polyanhydrides for controlled drug delivery” U.S.A.,

4,891,225, 1991 D'Amore, P., Leong, K., Langer, R., "Bioerodible articles useful as implants and prostheses having predictable

degradation rates” U.S.A. 4,886,870, Canada 507,997, Europe 0 246 341, 1991 Leong, K., "Biodegradable poly(phosphoesters) ” U.S.A. 5,256,765, 1993 Leong, K., "Biodegradable and elastomeric poly(phosphoester-‐urethanes) ” U.S.A. 5,176,907, 1993 Ni, N. and Leong, K., "Polymeric stationary phases for bioseparation and biosynthesis" U.S.A., 5,288,763, 1994 Wen, S. and Leong, K., "Living cells microencapsulated in a polymeric membrane" U.S.A., 5,620,883, 1997 Lo, H., Kadiyala, S., and Leong, K.,“Biodegradable foams for cell transplantation” U.S.A., 5,686,091, 1994, 1997 Azhari, R. and Leong, K., "Controlled release of proteins from complex coacervates" U.S.A., 5,759,582, 1998 Grossman, S., Lesser, G., Lo, H., and Leong, K., "Subcutaneous implant for delivery of hydromorphone" U.S.A.,

5,858,388, 1999 Pardoll D, Azhari R, Leong KW, Golumbek P, Jaffee E, Levitsky H, Lazenby A, “Controlled release of pharmaceutical active

substances for immunotherapy” 5,861,159, 1999 Mao HQ and Leong KW, “Biodegradable poly(phosphoester-‐co-‐desaminotyrosyl L-‐tyrosine ester) compounds” U.S.A.,

5,912,225, 1999 Truong, V., August, T., Leong, K., "Gene delivery system" U.S.A., 5,972,707, 1999 Zhong Z, Mao HQ, Leong KW, “Two-‐step polymerization for synthesis of high molecular weight poly(phosphoester)s”

U.S.A., 6,008,318, 1999 Grossman, S., Lesser, G., Lo, H., and Leong, K., "Subcutaneous implant" U.S.A., 6,126,956, 2000 Truong, V., August, T., Leong, K., "Solid microparticles for gene delivery" U.S.A., 6,025,337, 2000 Chen, X., J. Pasricha, Leong, K., “Urease-‐responsive delivery systems for diagnostic and therapeutic applications”

U.S.A., 6,156,346, 2000 Walsh S, Rubenstein R, Zeitlin P, Leong KW, “Therapeutic nanospheres” U.S.A., 6,207,195, 2001 Roy, K., Mao, H.Q., Truong, V., August, T., Leong, K., "Targeted gene delivery system" U.S.A., 6,410,517, 2002 Mao HQ, Lo H, Dang W, Zhao Z, Nowotnik D, English J, Leong KW, “Biodegradable terephthalate polyester-‐

poly(phosphonate) copolymers” U.S.A., 6,485,737, 2002 Mao HQ, Lo H, Dang W, Zhao Z, Nowotnik D, English J, Leong KW, “Biodegradable terephthalate polyester-‐

poly(phosphite) copolymers” U.S.A., 6,419,709, 2002 Dang W, Mao HQ, Kadiyala I, Leong KW, Zhao Z, English J, “Biodegradable compositions comprising

poly(cycloaliphatic phosphoester) compounds” U.S.A., 6,403,675, 2002


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Mao HQ, Zhao Z, English J, Leong KW, “Biodegradable polymers chain-‐extended by phosphates” U.S.A., 6,376,644, 2002

Roy K, Huang SK, Simpson H, Leong KW, “Oral delivery of nucleic acid vaccines by particulate complexes” U.S.A., 6,475,995, 2002

Mao HQ, Hendricks B, Lin K, Haller M, Leong KW, “Polymers for delivery of nucleic acids” U.S.A., 6,548,302, 2003 Mao HQ, Leong KW, Dang W, Lo H, Zhao Z, Nowotnik D, English J, “Biodegradable terephthalate polyester-‐

poly(phosphate) copolymers” U.S.A., 6,600,010, 2003 Haller M, LeVisage C, Malavaud B, Leong KW, “Systemic delivery of compounds through non-‐invasive bladder

administration” U.S.A., 6,797,704, 2004 Leong KW, Wen J, Zhuo RX, Mao HQ, Phosphate based biodegradable polymers, USA 6805876, October 19, 2004 Yu H, Leong KW, Chia SM, Wan ACA, Multi-‐layer cell encapsulation for tissue engineering. USA 6,916,640, July 12,

2005 Garver R, Kalyanasundaram S, Leong KW, Controlled release of bioactive substances. USA 6,916,490, July 12, 2005 Leong KW, Wen J, Mao HQ, Biologically useful polyphosphates. USA 6,852,709, February 8, 2005 Li W, Mao HQ, Leong KW, Controlled delivery of therapeutic agents by insertable medical devices. 6,899,731 May 31,

2005 Mohapatra S, Kumar S, Huang S, Leong KW, Gene expression vaccine. U.S.A., 7,118,888, October 10, 2006 Li J, Li X, Xu N, Leong KW, Biodegradable triblock copolymers, synthesis methods therefore, and hydrogels and

biomaterials made there from. USA 7,297,348 , November 20, 2007 Ohno T, Gang B, Leong KW, Liu SQ, Tumor vaccines. USA 7,247,310, July 24, 2007 Yu H, Chia S, Leong KW, Non-‐disruptive three-‐dimensional culture and harvest system for anchorage-‐dependent cells.

EP 1,326,968B1, April 18, 2007 Wang J, Mao HQ, Leong KW, Biodegradable polyphosphoramidates for controlled release of bioactive substances. USA 7,417,110 August 26, 2008 Wang J, Mao HQ, Leong KW, Biodegradable polyphosphates for controlled release of bioactive substances. USA 7,345,138 March 18, 2008 Salem A, Leong KW, Searson P, Methods and products for delivering biological molecules to cells using multicomponent nanostructures. USA 7,344,887 March 18, 2008 Mao, H-‐Q., Mun, C. and Leong, K., “Biofunctional Fibers” U.S.A. 7,524,513, April 28, 2009 Yu H, Leong KW, Chia SM, Wan ACA, Multi-‐layer cell encapsulation for tissue engineering. U.S.A. 7,943,353, May 17,

2011 Li J, Yang C, Li H, Wang X, Goh WH, Leong KW. Polycationic polyrotaxanes capable of forming complexes with nucleic

acids. U.S.A. 7,883,688, Feb 8, 2011 Liu Y, Wong KH, Sun G, He C, Leong KW. Polyalkyleneimine-‐graft-‐biodegradable polymers for delivery of bioactive

agents. U.S.A. 20110306657A1, Dec 15, 2011 Scott-‐Carnell L, Siochi EJ, Holloway NM, Leong KW, Kulangara K. Electroactive scaffold. U.S.A. 20110142806A1, Jun

16, 2011 Published patent applications: Williams J, Dang, W, and Leong KW, “Compositions for release of radiosensitizers, and methods of making and using the

same” 20020198135 Wen J, Mao HQ, Zhuo RX, Leong KW, “Phosphate based biodegradable polymers” 20020155092 Chia SM, Wan A, Yu Y, Leong KW, “Multi-‐layer cell encapsulation for tissue engineering” 20020094569 Li W, Mao HQ, Leong, KW, “Controlled delivery of therapeutic agents by insertable medical devices” 20020061326 Mao HQ, Wen J, Leong KW, “Biologically useful polyphosphates” 20020045263 Li J, Yu H, Leong KW, “Injectable drug delivery systems with cyclodextrin-‐polymer based hydrogels” 20020019369 Li J, Li X, Ni X, Leong KW, “Biodegradable triblock copolymers, synthesis methods therefore, and hydrogels and biomaterials


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made there from” 20040072799 Chia SM, Yu H, Leong KW. “A non-‐disruptive 3D culture and harvest system for anchorage-‐dependent mammalian cells”

Singapore Patent No: SG 96318 LeVisage C, Malavaud B, Haller M, Leong KW, “Polymer controlled delivery of a therapeutic agent” 20030008015 Wang S, Wan A, Yu H, and Leong KW, “Polymer and nerve guide conduits formed thereof” 20030060836 Mohapatra S, Kumar S, Huang S, Leong KW, “Gene expression vaccine” 20030068333 Yu H, Chia S, Leong KW, “Non-‐disruptive three-‐dimensional culture and harvest system for anchorage-‐dependent cells”

20040023370 Okoli G, Hortelano G, Leong KW, “Compositions of oral gene therapy and methods of using same” US20060051424A1 Filed patent applications: Kalyanasundaram S, Garver RI, Leong KW, “Controlled and targeted delivery of recombinant viral vectors” pending Truong V, August JT, Leong KW, “Genetic immunization with co-‐delivery of plasmid and cytokines in a single vehicle”

pending Salem A, Searson P, Leong KW, “Bi-‐segmental nanorods for gene delivery” pending Chua K, Mao HQ, Ramakrishna S, Leong KW, “Nanofibrous scaffold for hepatocyte culture” pending Wan A, Leong KW, “Biofunctional scaffold created by interfacial polyelectrolyte complexation” pending

Mao HQ, Kuan CM, Leong KW, “Biofunctional scaffolds for stem cell expansion and tissue engineering” pending

Dang JY, Leong KW, “Thermosensitive chitosan for tissue engineering applications” pending

Chew SY, Hoke A, Wang RF, Leong KW, “Electrospun fibers applied to peripheral nerve regeneration”

Liao IC, Leong KW, “Coaxial electrospun fibers and structures and methods of forming same related”

Zhao F, Leong KW. “Methods of Making Cell Sheet, Tissue Sheet and Tissue Engineered Blood Vessels.” No. 61/166,447. 2009

Bright C, Bright R, Churchill E, Leong KW, Rosen DM, Method and use of nano-‐scale devices for reduction of tissue injury in ischemic and reperfusion injury. US20070259032A1, November 8, 2007

Hoke A, Chen W, Leong KW, Chew SY, Mi R, Therapeutic electrospun fiber compositions. WO07089259A1, August 9, 2007

Wan A, Leong KW, Fiber constructs and process of fiber fabrication. US20070020244A1, January 25, 2007

Mohapatra S, Kumar M, Huang S, Leong KW, Behera A, Chen LC, de la Cruz C, Gene expression vaccine. US20070009951A1 , January 11, 2007

Mao, HQ, Leong KW, Chua KN, Seeram R, Compositions and methods for the expansion and differentiation of stem cells. USA 20080153163A1, June 26, 2008 Li J, Li X, Ni X, Leong KW, Biodegradable triblock copolymers, synthesis methods therefore, and hydrogels and biomaterials made there from. USA 20080057128A1, March 6, 2008 Sullenger BA, Oney S, Lam, TSR, Leong, KW, “A Method of modulating the activity of a nucleic acid molecule,” 60/920,807, 2009 Zhao F, Leong KW, “Methods of Making Cell Sheet, Tissue Sheet and Tissue Engineered Blood Vessels.” 61/166,447, 2009 Liao IC, Chew SY, “Leong KW, Coaxial electrospun fibers and structures and methods of forming the same.” 20100055154A1, March 4, 2010 Hoke A, Leong KW, Chew SY, Mi R. Therapeutic Electrospun Fiber Compositions. 20100303881A1, December 2, 2010 Scott-‐Carnell LA, Siochi EJ, Leong KW. Device and Method for Healing Wounds. 20100211151, August 19, 2010 Sullenger BA, Oney S, Lam TSR, Leong KW. Method of Modulating the Activity of a Nucleic Acid Molecule. 20100184822, July 22, 2010


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Liu Y, Wong KH, Sun G, He C, Leong KW. Polyalkyleneimine-‐graft-‐biodegradable polymers for delivery of bioactive agents. U.S.A. 20110306657A1, Dec 15, 2011 Scott-‐Carnell L, Siochi EJ, Holloway NM, Leong KW, Kulangara K. Electroactive scaffold. U.S.A. 20110142806A1, Jun 16, 2011 Quek CH and Leong KW. Quantum Dot Materials, Methods for Making Them, and Uses Thereof (Oct 3, 2011, U.S Prov. Pat. App. no. 61/542,603)


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Invited Presentations

“Implantable controlled drug delivery,” ACEMB, Lutherville MD, 1986 “New biodegradable polymers for orthopedic applications,” Walter Reed Army Research Institute, 1988 “Biodegradable poly(phosphate)s and poly(phosphonate)s as orthopedic prostheses,” Zimmer Corporate

Research Center, Warsaw, IN, 1988 “Poly(phosphate)s and poly(phosphonate)s for orthopedic and controlled release applications,” Whitaker

Foundation, 1988 “Poly(phosphate esters) as drug-‐carriers,” ACS Symposium on Drug Delivery, Dallas, 1989 “Polymeric controlled delivery of antisense,” NIH, 1989 “Recent developments of polymeric controlled drug delivery,” Institute of Polymer Chemistry, Nankai

University, China, 1989 “Biodegradable polymers for long bone fracture repair,” NIH, 1990 “Biodegradable elastomeric poly(phosphoester-‐urethane)s,” Johnson & Johnson, 1990 “Design of elastomeric poly(phosphoester-‐urethane)s for biodegradable stents,” Medtronics Corporate

Research Center, 1990 “Poly(phosphoesters) as Biomaterials,” ACS, Boston, 1990 “Poly(phosphoesters) as biomaterials: Controlled drug delivery and orthopedic applications,” Guilin

International Symposium on Biomaterials and Fine Polymers, 1991 “Polymeric controlled release device for restenosis,” Medtronics, Minneapolis, 1991 “Controlled release microspheres for osteoarthritis,” Osteoarthritis Sciences Inc., Cambridge MA, 1992 “Poly(phosphoesters) and poly(phosphoester-‐urethanes) in controlled drug delivery applications,”

American Society for Artificial Internal Organs, New Orleans, 1993 “Poly(phosphoesters) and poly(phosphoester-‐urethanes) in orthopedic and controlled drug delivery

applications,” Gordon Conference, San Miniato, Italy, 1993 “Designing polymers for controlled drug delivery,” Science Innovation 93, Boston, 1993 “Biodegradable polymers in orthopedic applications,” Symposium on Biological Response to Biomaterials,

Baltimore, 1993 “Alternative rodding materials for osteogenesis imperfecta,” Symposium on Osteogenesis Imperfecta,

Bethesda, 1993 “Chemical and mechanical considerations of biodegradable polymers for orthopedic applications,”

International Soc. Fracture Repair Workshop, Hong Kong, 1993 “Design of synthetic bone graft: BMP-‐containing biodegradable polymeric foam,” International Conf. on

Bone Morphogenetic Proteins, Baltimore, MD, 1994 “Biomedical applications of polymeric biomaterials (5 lectures),” Institute of Polymer Chemistry, Nankai

University, China, 1994 “Polymeric controlled drug delivery,” Guangzhou College of Pharmaceutics, China, 1994 “Synthesis and biomedical applications of poly(phosphoester)s (3 Lectures),” Department of Chemistry,

Wuhan University, China, 1994 “Synthesis and characterization of poly(phosphoester)s,” Department of Macromolecular Science, Fudan

University, China, 1994 “Microspheres and microcapsules for gene delivery and cell encapsulation,” International Symposium on

Biomaterials and Fine Polymers, Wuhan, 1994 “Role of polymer chemistry in tissue engineering,” Fall Meeting of CACS, Chicago, 1995 “New biodegradable polymers for drug delivery,” Skypharm, La Jolla, CA, 1995 “Drug delivery to the joint,” Keystone Conference on Drug Delivery, Hilton Head, 1995


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“A synthetic CFTR gene delivery system,” Cystic Fibrosis Foundation Conference, Williamsburg, 1995 “Delivery of cytokines by gelatin/chondroitin sulfate microspheres,” ACS Symposium on Protein Delivery,

Boston, 1995 “Biodegradable and macroporous polymeric scaffolds,” Keystone Conference on Tissue Engineering, Taos,

New Mexico, 1996 “Controlled release biodegradable scaffolds for bone regeneration,” AAAS Annual Meeting, Baltimore, 1996 “Urease-‐sensitive delivery system for diagnostic and therapeutic applications,” Boston Scientific,

Framingham, MA, 1996 “Urease-‐sensitive delivery system for diagnostic and therapeutic applications,” Abbott Laboratory, Chicago,

1996 “Synthesis of biodegradable and macroporous scaffolds for bone tissue engineering,” Osiris, Baltimore,

1996 “Polymeric controlled gene delivery,” Department of Bioengineering, University of Utah, 1996 “Polymeric controlled drug and gene delivery,” AAAS Annual Meeting, Philadelphia, 1996 “Biodegradable poly(phosphoester)s for drug and gene delivery applications,” Guilford Pharmaceuticals,

Baltimore, 1996 “Adjuvant anti-‐tumor effects of GM-‐CSF polymeric microspheres,” US-‐Japan Cancer Cooperative Research

Program on The Role of Cytokines in Cancer, Bethesda, 1996 “Design of new biodegradable hydrogels for controlled drug delivery,” BF Goodrich Corporate Research

Center, 1996 “Design and synthesis of enzymatically degradable polymeric biomaterials by group transfer

polymerization,” Medical Gel Sciences, Lexington, MA, 1996 “Polymeric controlled delivery of antigens,” Institute of Life Sciences, Zhongzhan University, 1996 “Macroporous scaffolds with controlled release functions applied to tissue engineering,” Department of

Bioengineering, Penn, 1997 “Polymeric controlled gene delivery: mechanism and genetic immunization,” Institute of Bioengineering

and Biosciences, Georgia Tech, 1997 “DNA nanospheres as non-‐viral vectors for gene delivery,” Eighth Conferences on Advances in Drug

Delivery, Salt Lake City, Utah, 1997 “Cystic fibrosis gene therapy by gelatin-‐DNA nanospheres,” Cystic Fibrosis Foundation Conference,

Williamsburg, 1997 “Polymeric carriers in non-‐viral gene therapy,” Department of Chemistry, Fudan University, China, 1997 “Biodegradable polymers for gene delivery and tissue engineering applications,” Fourth International

Symposium on Biomaterials and Fine Polymers, Xian, China 1997 “Recent trends of tissue engineering,” Institute of Materials Research and Engineering, Singapore, 1997 “Controlled release microspheres for cancer vaccination,” Workshop on Cancer Vaccines, Cambridge Health

Institute, Washington, D.C., 1997 “Application of controlled release technology for optimization of immune response,” NIH Workshop on

Interface of Bioengineering and Immunology, Bethesda, 1997 “Polymeric controlled non-‐viral gene delivery,” Transkaryotic Therapeutics, Boston, 1997 “Gene delivery properties of chitosan-‐DNA nanoparticles,” Korean Institute of Science and Technology,

Seoul, 1998 “Polymeric controlled gene delivery,” US-‐South Korea Cooperative Research Program on Biomedical

Engineering, Daejon, South Korea, 1998 “Genetic immunization by DNA nanoparticles,” Schering Plough Corporate Research Center, NJ, 1998 “Non-‐viral gene delivery by DNA nanoparticles,” Department of Pulmonary Medicine, Cornell Medical

Center, NY, 1998


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“Cancer vaccination and non-‐viral gene delivery,” Department of Anatomy, NUS, Singapore, 1998 “Genetic immunization with DNA-‐nanoparticles and cytokine adjuvants,” Institute of Molecular Cell

Biology, Singapore, 1998 “Bioengineering approach to gene therapy,” Faculty of Science, NUS, Singapore, 1998 “Mechanism and efficiency of gene transfection by DNA-‐nanospheres,” Keystone Symposia on Molecular

and Cellular Biology: Synthetic Non-‐Viral Gene Delivery Systems, Keystone, CO, 1998 “Recent trends of biodegradable scaffolding design in tissue engineering,” Guilford Pharmaceuticals,

Baltimore, 1998 “Polymeric controlled drug and gene delivery,” Omeros Medical System, Seattle, 1998 “Polymeric non-‐viral gene delivery,” Department of Medicine, Memorial Sloan Kettering Institute, 1998 “Non-‐viral gene delivery,” GeneMedicine, Woodlands, TX, 1998 “Polymeric controlled gene delivery,” Bohreinger Ingleheim, Connecticut, 1998 “Design of new polymeric gene carriers,” Gene Therapy, Inc., 1998 “Polymeric controlled gene delivery for immunotherapy,” 3rd Congress of European Association for Clinical

Pharmacology and Therapeutics, Jerusalem, Israel, 1999 “Mechanism and efficiency of gene transfer by DNA-‐nanoparticles ,” Department of Bioengineering, Rice

University, 1999 “Mechanism and efficiency of gene transfer by DNA-‐nanoparticles ,” Department of Chemical Engineering,

Caltech, 1999 “Supramolecular hydrogels for drug and gene delivery,” Omeros Medical System, Seattle, 1999 “Controlled release cytokine microspheres in DNA vaccination,” Alza, Palo Alto, 1999 “Non-‐viral gene delivery,” RPR Gencell, Mountain View, CA, 1999 “Bioengineering approach to gene delivery,” Bioengineering Program, Cornell University, 1999 “Biodegradable poly(phosphoester)s”, ACS Symposium honoring Robert Langer, Anaheim, 1999 “Controlled release approaches applied to cancer vaccination,” Symposium on Cancer Therapy, Johns

Hopkins-‐Singapore, Singapore, 1999 “Tissue engineering approach to intervertebral disc regeneration,” Workshop on Spinal Injury, Baltimore,

2000 “New tissue engineering scaffolding design and cell encapsulation technology,” Becton Dickinson Corporate

Research Center, Durham, NC, 2000 “Design of biodegradable polymeric biomaterials for gene delivery and tissue engineering,” Department of

Biomedical Engineering, Duke University, 2000 “Design of biomaterials, and drug and gene delivery related to tissue engineering,” Workshop of

Musculoskeletal Tissue Engineering, Hong Kong, 2000 “Oral gene therapy of food allergy,” NIH, 2000 “Biomaterials research in IMRE,” Johnson and Johnson Corporate Biomaterials Center, Somerville, NJ, 2000 “Polymeric biomaterials for drug delivery and tissue engineering,” DuPont Research Center, Wilmington,

2000 “Polymeric Controlled Gene Delivery,” International Meeting of BME, Singapore, 2000 “Non-‐viral Gene Delivery,” MRS Meeting, Boston, 2000 “Polymeric Controlled Gene Delivery,” University of Washington, Department of Bioengineering, 2001 “Biodegradable Polymeric Drug Carriers,” Alza Corp., Palo Alto, 2001 “Non-‐viral Genetic Immunization: Efficacy and Mechanism,” Merck, West Point, 2001 “Potential of Poly(phosphoester)s in Tissue Engineering Applications,” Guilford Pharmaceuticals,

Baltimore, 2001 “Biomaterials for Gene Delivery,” Society for Biomaterials Meeting, St. Paul, 2001 “Oral DNA Vaccines,” CRS Workshop, San Diego, 2001


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“Advances in Liver Tissue Engineering,” ICMAT, Singapore 2001 “New Biodegradable Poly(phosphoester)s for Gene Delivery,” ACS National Meeting, Chicago, 2001 “Polymeric controlled gene delivery,” Golden Gate Polymer Forum, San Francisco, 2001 “Oral gene delivery,” Virsol Scientific Meeting, Paris, 2001 “Polymeric controlled gene delivery,” 6th Symposium of Controlled Drug Delivery, Hawaii, 2001 “Nanoparticles in Biomedical Applications. Symposium on Nanoscience and Nanotechnology,” Singapore,

February, 2002 “ Polymeric Controlled Gene Delivery. Department of Biomedical Engineering,” Northwestern University,

March, 2002 “Polymeric Gene Carriers. Institute of Biomaterials and Biomedical Engineering,” University of Toronto,

Toronto, March, 2002 “Tissue Engineering Scaffold,” Department of Chemical Engineering, McMaster University, Hamilton,

March, 2002 “Oral gene delivery,” National Hemophilia Foundation Annual Meeting, Philadelphia. April 2002. “Polymer-‐DNA nanoparticles,” The 29th International Symposium on Controlled Release of Bioactive

Materials, Seoul, Korea. July 2002. “Biomaterials and Tissue Engineering,” University of Virginia, June 2002. “Drug and gene delivery in tissue engineering,” Challenges in Regenerative Medicine (ChaRM), Toronto,

June 2002. “Nanoparticle technology,” US-‐Taiwan Nanotechnology Summit, Caltech, September 2002. “Polyphosphoesters for drug delivery & tissue engineering,” Workshop on Polymeric Biomaterials: Design

& Applications, Wuhan, China. October 2002. “Biodegradable and biofunctional scaffolds for tissue engineering,” Plenary Speaker of 11th Annual

Scientific Meeting on Tissue Engineering, Kyungpook National University, Daegu, South Korea, December 2002

“Biofunctional scaffolds,” Symposium on Gels, Genes, Grafts, and Giants, Maui, Hawaii, December 2002 “Interface of Biomaterials and tissue engineering,” First World Congress of Chinese Biomedical Engineers,

Taipei, December 2002 “Polymeric controlled oral gene delivery,” Winter Symposium on Controlled Release of Bioactive Agents,

Utah, March, 2003 “Oral gene delivery,” IBC Symposium on Protein Formulation and Delivery, Boston, March, 2003 “Oral non-‐viral gene delivery,” Department of Pediatrics, University of Pennsylvania, June, 2003 “Nanoparticles for gene therapy,” US-‐Japan Symposium on Nanomedicine, Yokohama, Japan, October, 2003 “Polymeric controlled nonviral gene therapy,” NIH, November, 2003 “Biofunctional fibrous scaffolds,” Institute of Bioengineering and Nanotechnology, Singapore, December,

2003 “Liver tissue engineering,” A*STAR Symposium, Singapore, December, 2003 “Towards engineering of trachea tissue,” 1st International Conference on Epithelial Tissue Engineering,

Singapore, December, 2003 “Biomaterials approach to optimize hepatocyte culture,” 2nd International Conference on Materials

Advanced Technology, December, Singapore, 2003 “Nanorods for non-‐viral gene delivery,” 2nd International Conference on Materials Advanced Technology,

December, Singapore, 2003 “Delivery aspects of DNA vaccination,” Keystone Conference on Vaccines, January, Keystone, Colorado,

2004 “Nanoparticles in biomedical applications,” NIH Workshop on Interface of Nanotechnology and Cancer

Imaging,” January, Bethesda, Maryland, 2004


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“Polymeric controlled gene delivery,” Department of Chemical Engineering, Ohio State University, February, Columbus, Ohio, 2004

“Interface of controlled drug delivery and tissue engineering,” Annual Meeting of Society for Biomaterials, Sidney, May, 2004

“Biofunctional fibrous scaffolds for tissue engineering,” Wurzburg Conference on Tissue Engineering, Julius-‐Maximilians-‐University Würzburg, Germany, June, 2004

“Interface of controlled drug delivery and regenerative medicine,” National Science Council Symposium on Bioengineering, Taipei, Taiwan, June, 2004

“Interface of controlled drug delivery and regenerative medicine,” National Tsing Hua University, Hsin Chu, Taiwan, June, 2004

“Biofunctional fibrous scaffolds,” 9th Symposium on Biochemical Engineering, Tai Chung, Taiwan, June, 2004

“Controlled drug delivery applied to regenerative medicine,” 10th SCBA Symposium, Beijing, China, July, 2004

“Design of fibrous scaffolds for regenerative medicine,” International Conference of Bioengineering and Nanotechnology, Singapore, September, 2004

“Interface of controlled drug delivery and regenerative medicine,” Department of Biomedical Engineering, Purdue University, West Lafayette, Indiana, October, 2004

“Drug and gene delivery applied to regenerative medicine,” Department of Bioengineering, University of Illinois, Urbana-‐Champaign, Illinois, November, 2004

“Delivery of biologics from fibrous scaffold,” School of Pharmacy, University of North Carolina, Chapel Hill, North Carolina, November, 2004

“Nanoparticle technology applied to nonviral gene delivery,” NSF Center for Affordable Nanoengineering of Polymer Bioengineering, Ohio State University, February, 2005

“Cellular response to fibrous scaffolds with micro/nanoscale features,” Department of Chemical and Materials Engineering, University of California, Irvine, March 2005

“Bionanotechnology: Therapeutic applications,” GRC Keynote Lecture, U of Maryland, Baltimore and Baltimore County Campus, Baltimore, April, 2005

“Cellular response to biofunctional fibrous scaffolds,” Department of Biomedical Engineering, Duke University, April 2005

“Influence of nanostructures on cellular response,” AAPS Symposium on Nanomedicine, San Francisco, June 2005

“Controlled release micro/nano-‐ordered structures for tissue engineering,” Proceedings of International Conference on Controlled Release of Bioactive Agents, Miami, June 2005

“Influence of nanotopography on cellular behavior,” 3rd International Conference on Materials Applied Technology, Singapore, July, 2005

“Synthetic and natural biopolymers applied to tissue engineering scaffolding design,” Symposium on New Trends in Biomaterials—Tissue Engineering, National University of Singapore, Singapore, July 2005

“Interface of controlled drug delivery and tissue engineering,” Department of Biomedical Engineering, University of Minnesota, October, 2005

“Significance of nanostructures in dictating cellular behavior,” Department of Biomedical Engineering, Iowa State University, November, 2005

“Design of biofunctional fibrous scaffold for tissue engineering,” NUS-‐Tissue Engineering Workshop, Singapore, December, 2005

“Influence of nanotopographical cues in stem cell differentiation,” Plenary Lecture, 12th International Conference of Biomedical Engineering, Singapore, December, 2005

“Significance of nanostructures in dictating cellular behavior,” Pacific Polymer Conference IX, Maui, Hawaii, December, 2005


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“Nanotherapeutics: application of nanotechnology to gene and cell therapy,” Center for Bioinspired Materials and Material Systems, Duke University, January, 2006

“Nanotechnology applied to gene and cell therapy,” National Heart, Lung, and Blood Institute of NIH, Bethesda, April, 2006

“Biofunctionality derived from continuous nanostructures,” 1st Chapel Hill Drug Conference, Chapel Hill, June 2006

“Applications of nanomedicine,” Becton Dickinson Technology, Durham, July, 2006 “Nanotherapeutics: application of nanotechnology to gene and cell therapy,” Key Laboratory of Biomedical

Polymers, Wuhan University, China, August, 2006 “Nanotherapeutics: application of nanotechnology to gene and cell therapy,” Polymer Chemistry Institute,

Nankai University, China, August, 2006 “Application of nanotechnology to medicine,” Peking Union Medical College Hospital, Beijing, China, August,

2006 “Nanotherapeutics: application of nanotechnology to gene and cell therapy,” Department of Biomedical

Engineering, Peking University, Beijing, China, August, 2006 “Nanotherapeutics: application of nanotechnology to drug, gene and cell therapy,” Cancer Center Oncology

Symposium, Duke University, October, 2006 “Nanotechnology applied to gene and cell therapy,” Pulmonary Research Conference, Duke University,

October, 2006 “Nanotherapeutics: application of nanotechnology to gene and cell therapy,” School of Pharmaceutical

Sciences, University of Wisconsin, Madison, November, 2006 “Biofunctionality derived from continuous nanostructures,” 1st Cancer Nanotechnology Symposium,

University of North Carolina, Chapel Hill, November, 2006 “Biofunctionality derived from polymeric continuous nanostructures,” NANOBIO Tokyo-‐2006, Tokyo,

Japan, December, 2006 “Delivering biochemical and topographical cues by polymeric continuous nanostructures,” Department of

Biotechnology, University of Malaya, February, 2007 “Response of stem cells to continuous nanostructures,” NIH-‐Specialized Cooperative Centers in

Reproduction and Infertility Research, Portland, Oregon, May, 2007 “Novel design of biofunctional contact lens,” Bausch & Lomb Corporation, Rochester, New York, May 2007 “Nanotherapeutics: Application to gene and cell therapy,” Samyang Corporation, Seoul, South Korea, June,

2007 “Response of stem cells to nanostructures,” ACS-‐Polymers in Medicine and Biology, Sonoma, California,

June, 2007 “Nanotherapeutics: Application of nanotechnology to gene and cell therapy,” Global Enterprise for Micro-‐

Mechanics and Molecular Medicine—Cancer Conference, Singapore, June, 2007 “Application of quantum-‐dot FRET to investigate nonviral gene transfer,” Keynote Speaker, International

Conference of Materials Advanced Technology, Singapore, July, 2007 “Delivering biochemical and topographical cues by polymeric continuous nanostructures,” Keynote

Speaker, 3rd International Conference on Bioengineering and Nanotechnology, Singapore, July, 2007 “Nonviral oral gene delivery for hemophilia therapy,” Durham VA Medical Center, Durham, August, 2007 “Biofunctionality derived from polymeric continuous nanostructures for tissue engineering,” Materials

Today Asia Conference, Beijing, China, September, 2007 “Nanotherapeutics,” Institute of Life Sciences, University of Science and Technology, Hefei, China,

September, 2007 “Nanotherapeutics: Application of nanotechnology to gene and cell therapy,” Department of Biomedical

Engineering, Tsinghua University, Beijing, September, 2007


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“Nanotherapeutics: Application of nanotechnology to gene and cell therapy,” William Monk Distinguished Lectureship, University of Hong Kong, Hong Kong, September, 2007

“Biofunctional polymeric continuous nanostructures,” Hong Kong University of Science and Technology, Hong Kong, September, 2007

“Nonviral gene transfer mediated by DNA nanoparticles,” Keynote Speaker, 57th Canadian Chemical Engineering Conference, Edmonton, Alberta, October, 2007

“Biofunctional polymeric continuous nanostructures,” School of Pharmaceutical Sciences, Wayne State University, October, 2007

“Delivering biochemical and topographical cues from continuous nanostructures,” Department of Bioengineering, University of California, Berkeley, October, 2007

“Delivering biochemical and topographical cues from continuous nanostructures,” DB Robinson Distinguished Speaker, University of Alberta, November, 2007

“Nonviral gene transfer mediated by DNA nanoparticles,” Nanotechnology in Biology and Medicine Conference, Charlotte, North Carolina, November, 2007

“Mechanism of nonviral oral gene delivery,” National Institute of Biomedical Imaging and Bioengineering, Bethesda, November, 2007

“Stem cell response to polymeric continuous nanostructures,” Plenary Speaker, First International Conference on Biomolecular Cellular Engineering, Singapore, December, 2007

“Stem cell response to polymeric nanostructures,” Department of Biological Engineering, M.I.T. Boston, February, 2008

“Nano and ultrafine particles: Adverse effects on health”, Annual Meeting of American Academy of Allergy Asthma and Immunology, Philadelphia, March, 2008

“Chitosan for oral delivery of nucleic acids”, Annual Meeting of American Academy of Allergy Asthma and Immunology, Philadelphia, March, 2008

“Nonviral oral gene delivery”, 10th European Society of Controlled Drug Delivery Symposium, Amsterdam, April, 2008

“Optimizing gene delivery with quantum dot-‐FRET technology”, Center for Nanotechnology Science and Technology, University of Illinois, Urbana Champaign, April 2008

“Identifying nonviral gene transfer barriers by quantum dot-‐FRET technology”, Institute of Bioengineering and Nanotechnology, Singapore, May 2008

“Optimizing gene delivery with quantum dot-‐FRET technology”, Keynote speaker, 3rd International Symposium of Biomedical Engineering and Bionanotechnology, Changsha, China, June 2008

“Nanotherapeutics”, Vertex Pharmaceuticals, Boston, June 2008 “Response of stem cells to polymeric continuous nanostructures”, Gordon Research Conference on Signal

Transduction by Engineered Extracellular Matrix, Portland, Maine, July 2008 “Nanostructured biomaterials applied to regenerative medicine”, Center for Integration of Medicine and

Innovative Technology, Boston, October 2008 “Cell-‐based intervertebral disc therapy with scaffold mediation”, University of Zurich, Zurich, Switzerland,

November 2008 “Biomaterials-‐assisted therapy of intervertebral disc degeneration”, AOSpine International, Zurich,

Switzerland, November, 2008 “Optimizing gene delivery with quantum dot-‐FRET technology”, Second International Research Network

Symposium, Seoul, S. Korea, December, 2008 “Nanostructured biomaterials”, Keynote speaker, A*STAR Workshop on Biomaterials, Singapore,

December, 2008 “Nanostructured biomaterials: Relevance to regenerative medicine and tissue biocompatibility”, Keynote

speaker, Biomaterials Asia, Hong Kong, April, 2009 “Stem cell tissue engineering”, Department of Medicine, University of Hong Kong, April, 2009


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“Nonviral gene carrier design aided by QD-‐FRET”, Center for Biologically Bioinspired Materials and Material Systems, Beaufort, NC, May, 2009

“Nanostructured biomaterials: Relevance to regenerative medicine and tissue biocompatibility”, Wake Forest Institute of Regenerative Medicine, Winston-‐Salem, NC, May, 2009

“Tissue-‐engineered intervertebral disc—is this possible?” Global Spine Congress, San Francisco, June, 2009 “Nanostructured biomaterials: Relevance to regenerative medicine and tissue biocompatibility”, Gordon

Research Conference, Holderness, NH, July, 2009 “Relevance of microfluidic platforms to nanomedicine”, Nano Today 1st International Conference,

Singapore, August, 2009 “Nanostructured biomaterials: Relevance to regenerative medicine and tissue biocompatibility”, School of

Pharmacy, University of Tennessee, Memphis, TN, Sept, 2009 “Convergence of microfluidics and nanophotonics for gene delivery”, Ohio State University, Columbus, OH,

Oct, 2009 “Cancer therapy: challenges of delivery”, NSF-‐Mauritius Workshop on Biomaterials: Perspectives and

Opportunities, Mauritius, Dec, 2009 “Gene therapy: barriers of nonviral delivery”, NSF-‐Mauritius Workshop on Biomaterials: Perspectives and

Opportunities, Mauritius, Dec, 2009 "Microfluidic platforms related to nanomedicine", Keynote Speaker, 8th iNano Meeting, University of

Aarhus, Denmark, Feb, 2010 "Response of stem cells to nanotopographical cues and vascular tissue engineering," Keynote Speaker,

NanoBio Collaborative Conference, University of South Florida, Tempa, FL, Mar, 2010 "Microfluidic platforms related to nanomedicine", Keynote Speaker, Symposium on the Convergence of

Nanotechnology and Life Sciences, SUNY at Albany, Albany, NY, Mar, 2010 "Microfluidic platforms related to nanomedicine", Keynote Speaker, FNANO Symposium, Snowbird, UT,

April, 2010 “Optimization of oral nonviral gene delivery”, Department of Pharmacology, School of Medicine, University

of Pennsylvania, PA, April, 2010 “Optimization of oral nonviral gene delivery”, Institute of Life Sciences, University of Science and

Technology of China, Hefei, China, June, 2010 “Optimization of oral nonviral gene delivery”, Key Laboratory of Biomedical Polymers of Ministry of

Education, Department of Chemistry, Wuhan University, Wuhan, China, June, 2010 “Response of stem cells to nanotopography: tissue-‐engineered blood vessels”, Key Laboratory of

Biomedical Polymers of Ministry of Education, Department of Chemistry, Wuhan University, Wuhan, China, June, 2010

“Optimization of oral nonviral gene delivery”, Institute of Chemistry, Chinese Academy of Sciences, Beijing, China, June, 2010

“Biological treatment of intervertebral disc degeneration: controlled delivery technologies”, AOSpine World Congress, Montreal, Canada, July, 2010

“Nanostructured biomaterials: relevance to regenerative medicine and tissue biocompatibility”, Keynote Lecture, American Society of Nanomedicine, Bethesda, NIH, Oct 2010

“Nanostructured biomaterials”, Keynote Lecture, Johnson and Johnson Nanotechnology Symposium, New Brunswick, Oct 2010

“Nanostructured biomaterials: relevance to regenerative medicine and tissue biocompatibility”, Keynote Lecture, Society of Biomaterials, Biomaterials Day at Johns Hopkins University, Baltimore, MD, Oct 2010

“Optimizing Gene Delivery with QD-‐FRET and Microfluidics-‐mediated Self-‐assembly”, Distinguished Lecture Series, NSF Center for High-‐Rate Nanomanufacturing, Northeastern University, Boston, Nov, 2010

“Cellular response to topographical cues”, Keynote Lecture, Molecular Nanotechnology Symposium, Nara, Japan, Dec 2010


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“Microfluidics-‐assisted synthesis of DNA nanocomplexes”, Symposium on Recent Advances of Drug Delivery, Salt Lake City, Utah, Feb 2011

“Optimization of nonviral gene delivery by QD-‐FRET technology”, Center for Nanotechnology, University of Washington, Mar 2011

“Relevance of topographical cues to regenerative medicine”, Department of Bioengineering, University of Washington, Mar 2011

“Nanotherapeutics: applications of discreet and continuous nanostructures,” Distinguished Karcher Lecture Series, Department of Chemistry and Biochemistry, University of Oklahoma, Mar 2011

“Self-‐assembly of polyplexes in picoliter volume,” 15th International Symposium on Recent Advances in Drug Delivery Systems, Salt Lake City, Utah, Mar 2011

“Microfluidics-‐mediated synthesis of polyplexes and their applications,” School of Pharmacy, UNC Chapel Hill, Apr 2011

“Stem cell response to nanostructured biomaterials,” Gordon Research Conference on Environmental Nanotechnology, Waterville, NH, Jun 2011

“Cellular response to topographical cues: Relevance to regenerative medicine and nonviral transfection,” National University of Ireland, Galway, Ireland, Jun 2011

“Microfluidics-‐mediated synthesis of DNA/RNA polyplexes,” Controlled Release Society Annual Meeting, Washington DC, Aug 2011

“Cellular response to continuous nanostructures”, Keynote Lecture, 5th WACBE Symposium, Tainan, Taiwan, Aug 2011

“Addressing barriers of nonviral gene delivery by QD-‐FRET and microfluidic technologies,” National Kaohsiung University, Kaohsiung, Taiwan, Aug 2011

“Cellular response to topographical cues”, ACS Symposium on Polymer in Medicine, Santa Rosa, CA, Sept 2011

“Cellular Response to Nanotopographical Cues: Relevance to Regenerative Medicine and Nonviral Gene Transfer”, Russian 2nd Nanomaterials Conference, Moscow, Sept 2011

“Role of cell-‐topography effects in nonviral gene delivery”, 10th NHLBI Symposium on Gene Therapy, Sonoma, CA, Nov 2011

“Nanotherapeutics for genetic medicine”, Distinguished Lectures in Life Sciences Series, Pennsylvania State University, College Station, PA, Nov 2011

“Microfluidics-‐mediated synthesis of DNA and RNA polyplexes”, Keynote Lecture, 2nd Nano Today Conference, Hawaii, Dec 2011

“Nanotherapeutics: applications of discreet and continuous nanostructures,” University of Missouri, Kansas City, School of Pharmacy, Kansas City, Jan 2012

“Nanotherapeutics for genetic medicine”, Tianjin Medical University, Tianjin, China, March 2012 “Cellular Response to Nanotopographical Cues: Relevance to Regenerative Medicine and Nonviral Gene

Transfer”, Department of Biomedical Engineering, Cornell University, April 2012 “Nanostructured biomaterials for gene and cell therapy”, Department of Chemical and Biomedical

Engineering, Arizona State University, April 2012 “Implications and applications of cell-‐topography interactions”, Biointerface Gordon Research Conference,

Les Diablerets, Switzerland, May 2012 “Response of stem cells to topography cues”, Keynote Lecture, 9th World Congress for Biomaterials,

Chengdu, China, June 2012 “Nanotherapeutics: Applications of discrete and continuous nanostructures for gene and cell therapy”,

Plenary Lecture, International Union of Materials Research Societies, Singapore, July 2012 “Mechanistic understanding of cell-‐topography interactions”, Engineered Extracellular Matrix Gordon

Research Conference, Biddeford, Maine, July 2012 “Microfluidics-‐mediated synthesis of DNA/RNA polyplexes”, NanoBio Seattle, Seattle, Washington, July

2012


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“Nanotherapeutics for genetic medicine”, College of Medicine, National Taiwan University, Taipei, Taiwan, August 2012

“Implications and applications of cell-‐topography interactions”, New Jersey Symposium of Biomaterials, Rutgers University, October 2012

“Implications and applications of cell-‐topography interactions”, Clemson Award Lecture, Annual Meeting of Society for Biomaterials, New Orleans, Louisiana, October 2012

“Microfluidics-‐mediated synthesis Nanocomplexes”, International Society for the Study of Xenobiotics, Dalls, Texas, October 2012

“Influence of cell-‐topography interactions on stem cell tissue engineering”, Department of Molecular Pharmaceutics, UNC-‐Chapel Hill, October 2012

“Implications and applications of cell-‐topography interactions”, Department of Chemical Engineering, Texas Tech University, November 2012

Documents

Kam W. Leong, Ph.D. James B. Duke Professor Department of