MiSeq 시스템 가이드 - Illumina, Inc. · 2021. 1. 7. · MiSeq Reporter를 다시 추가하고 Local Run Manager 관련 내용 삭제. 문서의 제목을 'MiSeq 시퀀싱 시스템

MiSeq 2021 1
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MiSeq
. .
: 20000262 : 15027617 v06
2021
1 MCS v4.0 Local Run Manager v3.0 .
Windows 10 .
UI . . BaseSpace Onsite . Illumina Experiment Manager . MiSeq Reagent Kits v1 .
: 20000262 : 15027617 v05
2019
6
2018
7 Local Run Manager, Sample Sheet Manual .
MiSeq Reporter Local Run Manager .
' ' Reagent Chiller .
: 20000262 : 15027617 v03
2018
5 OS . .
' ' Illumina Proactive . Reagent Chiller ' ' .
MiSeq Reporter Local Run Manager . 'MiSeq ' 'MiSeq ' . .
: 20000262 : 15027617 v02
2018
1 Local Run Manager, Sample Sheet Manual .
MiSeq Reporter Local Run Manager .
' ' Reagent Chiller .
' ' . 'MiSeq ' 'MiSeq ' .
: 15027617 v06 KOR : 20000262 iii
. .

: 20000262 : 15027617 v01
2015
9
'MiSeq ' 'MiSeq ' .
MCS v2.6 BaseSpace Onsite . .
Welcome Home . , , MiSeq Reagent Kit
Reagent Prep Guides . MiSeq Reagent Kit Illumina (support.illumina.com/
sequencing/sequencing_kits/miseq_ reagent_kit.html) . 17.25 ml 51.75 ml . . ' A' . ' B' . ' ' . '' '' .
' ' '' .
1 RTA . 6% NaOCl 5%
NaOCl . 5% NaOCl .
: 15027617 Rev. O 2014
9

• MCS v2.5 .
• (NaOCl) .
• . , , 2 , VeriSeq
PGS .
: 15027617 Rev. N 2014
6 VeriSeq PGS . . .
MiSeq .
MiSeq
. .
: 15027617 Rev. M 2014
1 MCS v2.4 . Bundle Logs .
: 15027617 Rev. L 2013
10
. .
MiSeq . .
MiSeq Reagent Kit .
MiSeq Reagent Kit Reagent Kit . Reagent Preparation Guide . .
: 15027617 Rev. K 2013
8
: 15027617 Rev. J 2013
8 MCS v2.3 MiSeq Reagent Kit v3 .

• MiSeq Reagent Kit v3 .
• v3 .
• v2 v3 .
• .
Nano Flow Cell(D) Micro Flow Cell(G) . , MiSeq Reagent Kit . MiSeq
Reagent Preparation Guide( : 15044983) .
: 15027617 Rev. H 2013
3
, manifest , MiSeq .
FASTQ , manifest , , . MiSeq Reporter User Guide( : 15028784) MiSeq Sample Sheet Quick Reference Guide( : 15028392) . . Using Custom Primers on the
MiSeq( : 15041638) .
. .

: 15027617 Rev. G 2013
1 DNA Illumina PhiX Control .
Preparing DNA Libraries for Sequencing on the MiSeq( : 15039740) .
25 ml 10% Tween 20 500 ml 475 ml .
: 15027617 Rev. F 2012
11

• MiSeq Reagent Nano Kit MiSeq Reagent Micro Kit .
• .
• Enrichment .

• MCS v2.1
Perform Wash .
• Nano Kit Micro Kit .
• Reagent Kit .
: 15027617 Rev. E 2012
10

• PhiX Control PhiX Control
1000~1200 K/mm² .
• Nextera XT TruSeq
Amplicon .
• MiSeq Expansion Pack MiSeq Hardware Upgrade .
• MiSeq Reporter User Guide .
MiSeq
. .

: 15027617 Rev. D 2012
7 MCS v2.0 .

• ,
MCS .
• MiSeq Reagent Kit v2(500 cycles) .
• .
• ( 14) MiSeq Expansion Pack .
• .
• Nextera XT PCR Amplicon .
• 10% Tween 20 .
• RFID .

• IMF, CMF, AMX v2 IMS, CMS, AMS .
• PhiX 8 pM 12.5 pM .
• NaOH 1 nM .
• Standby Mode .
• Sample Sheet Parameters . . MiSeq Sample Sheet Quick Reference Guide( : 15028392) Illumina Experiment Manager User Guide( : 15031335) .
MiSeq
. .

: 15027617 Rev. C 2012
4 MCS v1.2 . .
BaseSpace , , FASTQ , , , , Idle Mode ( )

• Amplicon Custom Amplicon . De Novo Assembly Assembly . Generate FASTQ .
• . . .
• ' ' .
• NaOH .
• MiSeq Self-Service 'RFID ' .
• .
: 15027617 Rev. B 2011
12 MCS v1.1 . .

• .
• .
• — ' ' .
• — PR2 '' .
• — 2 .
• — TruSeq Custom Amplicon manifest .
• 'MiSeq Reagent Kit ' HT1 .
• iCom MyIllumina .
: 15027617 Rev. A 2011
9
. .

. .
4 .................................................................................................30 ..................................................................................................................................30
Local Run Manager ............................................................................................43 ........................................................................................................................43
. .
1 ..................................................................................................................................................................... 1

Illumina® MiSeq™ sequencing by synthesis(SBS) 8 DNA . MiSeq , .

X — , (run) .
X — . (paired-end) , . RFID(radio-frequency identification, ) .
X — MiSeq Control Software(MCS) , , .
X — (clamping mechanism) . .
X (fluidics) — MiSeq chemistry cycle time( ) .
X Real-Time Analysis(RTA) — (base calling) (downstream analysis) .
X Local Run Manager — (alignment) RTA 2 .
Illumina MiSeq System Support . , , . Support .
: 15027617 v06 KOR : 20000262 1
. .

Customer Protocol Selector , (run) .
MiSeq ( : 15027615) , .
MiSeq ( : 15027616) , , .
MiSeq Sample Sheet
.
MiSeq System Denature and Dilute Libraries Guide( : 15039740) MiSeq PhiX Control . .
MiSeq Custom Primers Guide( : 15041638) .
Local Run Manager v3 ( : 1000000111492) Local Run Manager , .
BaseSpace User Guide( : 15044182) BaseSpace .
MiSeq , , USB .
MiSeq
. .
A — (flow cell stage) . , .
B — .
C — (), () () .
D — Control Software .
E USB — .
F — , . .
MiSeq . .
, (thermal station) . , . .
.
1
A
. .
B
C
E
Reagent Chiller, PR2( ) . Reagent Chiller .

Reagent Chiller 2~11°C.
Reagent Chiller . Reagent Chiller (wash tray) . (sipper) .
Reagent Chiller PR2 . PR2 . . .
2
A Reagent Chiller
C PR2
E
MiSeq MiSeq .

Analysis Workflow 2 Local Run Manager . .
MiSeq
. .

Manifest Manifest . manifest manifest MCS Manifest . Manifest 2 Local Run
Manager .
Reference Genome FASTA . .
Run Folder RTA (MiSeqOutput ) Local Run Manager (MiSeqAnalysis ) . 44 .
Sample Sheet (*.csv) . MiSeq . SampleSheet.csv MiSeqTemp,
MiSeqOutput MiSeqAnalysis .
manifest Local Run Manager v3 ( : 1000000111492) .
MiSeq Sample Sheet Quick Reference Guide( : 15028392) .
MiSeq Software Suite , .
X MiSeq Control Software(MCS) — MCS . MCS . .
X MCS , , , . MCS Local Run Manager .
X Real-Time Analysis(RTA) — RTA (quality score) . RTA RTA .
X Local Run Manager — Local Run Manager , , , . Local Run Manager . Windows . 7 Local Run Manager .
Off-instrument ( ) Sequencing Analysis Viewer(SAV) . 7 Sequencing Analysis Viewer .
MiSeq
. .
Control Software . .
. . Acknowledge Close .
. .

OK . .
, .
.
.
3
X Reagent Chiller (°C)
X (°C)
X BaseSpace® (BaseSpace 6 BaseSpace )
2 MiSeq Local Run Manager , BaseSpace . , (contig, ) .
BaseSpace BaseSpace® Illumina .
BaseSpace . BaseSpace . 11 .
MiSeq
. .
MiSeq BaseSpace .
BaseSpace , .
BaseSpace RTA MiSeq . , .
BaseSpace 2 . BaseSpace Local Run Manager .
basespace.illumina.com BaseSpace . MyIllumina . BaseSpace BaseSpace User Guide( : 15044182) Illumina BaseSpace Support .
Local Run Manager Local Run Manager , , . MCS , . Local Run Manager 2 .
Local Run Manager (positive sample) .
Local Run Manager , . MiSeq . Local Run Manager . Local Run Manager v3 ( : 1000000111492) .
MiSeq .
Local Run Manager 2 MiSeq . , 2 .
2 .
Sequencing Analysis Viewer Illumina Sequencing Analysis Viewer(SAV) . SAV 1 MiSeq .
SAV (metrics) . SAV MiSeq . SAV . (Output folder) .
(template) SAV RTA , , .
MiSeq
. .
SAV Illumina ( ) . 1~8 . MiSeq All Lane 1 . Sequencing Analysis Viewer User Guide( : 15020619) .
550 GB.
. .
. 35 Manage Files . Restart Check .
MiSeq Reagent Kit MiSeq Reagent Kit 1 . MiSeq Reagent Kit . MiSeq Reagent Kit .
, PR2 (RFID) .
. .
Illumina MiSeq Reagent Kits Product .
MiSeq .
. .
.
4 MiSeq
A
B
C
MiSeq
. .

Standard Flow Cell
PGS Flow Cell
Micro Flow Cell
Nano Flow Cell
MiSeq Reagent Cartridge .
. Load Samples 17 .

. , , , . , , . . , , support.illumina.com/sds.html (Safety Data Sheet, SDS) .
MiSeq
. .
1

17 Reserved Load Sample( )
18 Reserved Read 1 [ ]
19 Reserved Index Read [ ]
20 Reserved Read 2 [ ]

MiSeq Reagent Cartridge MiSeq Custom Primers Guide( : 15041638) .
MiSeq
. .
2 MiSeq ........................................................................................................................................................11
.................................................................................................................................................................13
MiSeq 1 |() .

, 36 . 60 .
6
2 OS . . OS MiSeq Control Software(MCS) .
3 Local Run Manager User Management Local Run Manager Next .
1 Main Menu System Settings .
2 Run .
3 Post Run Wash Maintenance Wash . . . Post- Run Wash . 30 . 90 .
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. .
4 [ ] Start run after pre-run check. Do not prompt for confirmation. .
5 BaseSpace .
6 BaseSpace . Illumina Proactive Support .
7 Illumina Proactive Send Instrument Performance Data to Illumina to aid technical support . MCS . Illumina . Illumina , . Illumina Proactive Technical Note( : 1000000052503) .
Illumina Proactive
X Illumina . X . X . Send Instrument Performance
Data to Illumina to aid technical support .
BaseSpace 1 Main Menu Software Update .
2 Automatically check for new software updates on BaseSpace .
RTA , 2 , MiSeq MiSeq .
2 Email Notifications .
3 .
X Local SMTP email server address — SMTP . SMTP .
X Sender address — . .
X Recipient addresses — . . Test .
X Notify via email when — .
.
2 Folders .
MiSeq
. .
X Output Folder — . . 44 .
X Sample Sheet Folder — . .

.

Stock
1.0 N NaOH PhiX Control DNA
, 70% 70%
VWR( : 95041-714*)

(powder-free)
(low-lint) VWR( : 21905-026*)
10 x 15 cm VWR( : 52846-001*)
PhiX Control DNA
MiSeq tubes Illumina
( : MS-102-9999)
VeriSeq PGS ( )
5% NaOCl Sigma-Aldrich( : 239305*) VeriSeq PGS ( )
Tween 20 Sigma-Aldrich( : P7949)
()

*
(deionized water, DIW) . . .
X (DIW)
. .
3 ....................................................................................................................................................................14
........................................................................................................................................................14
MiSeq .
.
X Local Run Manager — Local Run Manager .
X Sample Sheet — . (validation) Local Run Manager .
X Manual — 10 . 2 .
.
.
X BaseSpace Sequence Hub.
X Sequencing .
X Sequencing Analysis Viewer(SAV) . , Illumina .
X Local Run Manager .
.
. (paired-end) 2 x 301 MCS v2.3 Index Read .
MiSeq .
Illumina Specifications for the MiSeq System .
: 15027617 v06 KOR : 20000262 14
. .
(read) 1 . (phasing) (prephasing) .
, 300 301 (, 2 x 301) 602. 2 x 300 .
DNA (bridge amplification).

LED 4 (dideoxynucleotide, ddNTP) . 1 . . , .

Local Run Manager Sample Sheet 2 Local Run Manager . 2 . 17 Local Run Manager .
.

2~8°C . 1 .
1 -25~-15°C .
2 DIW . .
7
3 .
X MiSeq v3 — 60~90 X MiSeq v2 — 60
4 . .
MiSeq
. .
1 10 .
2 1, 2 4 , .
3 .

MiSeq .
4 6 2~8°C . .

PhiX Control( ) . MiSeq System Denature and Dilute Libraries Guide( : 15039740) . VeriSeq PGS VeriSeq PGS Library Preparation Guide( : 15052877) .
. Illumina Sample Prepration Method . Sample Prepararation Guide .

MiSeq Custom Primers Guide( : 15041638) .
.
1 (low-lint) Load Samples .
2 1 ml .
3 600 μl Load Samples . .
MiSeq
. .
4 MCS .
MCS 1 Main Menu Reboot .
2 [ ] Folders MiSeqOutput, Recipe, Sample Sheet Manifest . 12 .
3 Home Sequence . Home Sequence Sequence Mode Selection(Local Run Manager, Sample Sheet, Manual), BaseSpace Option, Load Flow Cell, Load Reagents, Review, Pre-Run Check .
Local Run Manager 1 Home Sequence .
2 Sequence Mode Selection Local Run Manager .
3 [ ] BaseSpace Options Use BaseSpace™ . 18 BaseSpace .
4 Next .
5 .
6 [ ] Preview Samples .
7 Next 20 .

. 19 .
Sample Sheet 1 Home Sequence .
2 Sequence Mode Selection Sample Sheet .
3 [ ] BaseSpace Options Use BaseSpace™ . 18 BaseSpace .
MiSeq
. .
4 Next .
5 . Local Run Manager .
6 [ ] Local Run Manager 2 Disable Local Run Manager Secondary Analysis .
7 .
8 Next 20 .

. 19 .
BaseSpace BaseSpace 2 ( ).
Local Run Manager Mode Sample Sheet Mode BaseSpace 2 .
1 [ ] BaseSpace Options Use BaseSpace™ . raw data MiSeq .
2 .
X Send all run data to BaseSpace Sequence Hub for remote monitoring and data analysis. X Only send run information files to BaseSpace Sequence Hub so runs can be monitored
remotely.
4 Next .
2 Sequence Mode Selection Manual .
3 [ ] Use run monitoring only MyIllumina .

Manual Mode BaseSpace Sequence Hub Analysis and Storage .
4 Next .
5 Read Type, Read Length Custom Primers . Index Read 10 .
6 Next 20 .

. 19 .
MiSeq
. .
1 (powder-free) .
2 .
9
3 . . .
10
4 . .
11
5 . , , , .

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6 .
7 . .
1 . .
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2 . . .
3 .
14
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5 RFID 41 RFID .
6 .
7 Next .

PR2 1 2~8°C PR2 . .
2 .
3 .
4 PR2 .
16 PR2
5 .
6 . PR2 .
MiSeq
. .
17
7 PR2 RFID 41 RFID .
8 Next .

Reagent Chiller .
2 Illumina Reagent Chiller . . . Back Home Home .
18
3 Reagent Chiller .
4 RFID 41 RFID .
MiSeq
. .
5 . Back Exit Home .
6 .
7 Next .
.

Run Settings Start run after pre-run check . 11 .
1 Experiment Name, Module Name, Read Type, Read Length Custom Primers . .
2 [ ] Local Run Manager Mode Manual Mode Edit Read Type, Read Length Custom Primers Save .

. Sample Sheet Mode .
3 Change Folder .
4 Save and Continue .
5 Next .
Change Folder . Review .
, .
. 41 .
Start Run .
MiSeq
. .

MiSeq . .
Start Run . 40 .

MiSeq , .
Sequencing . Sequencing .
.
X Sequencing Analysis Viewer(SAV) . . 7 Sequencing Analysis Viewer . BaseSpace BaseSpace SAV .
X Local Run Manager . Local Run Manager (: Total Clusters, % Clusters PF, Read 1 and Read 2%>= Q30, Last Scored Cycle) . 7 Local Run Manager .
1 Sequencing , .
X Run Progress — . X Intensity — 90 .
Intensity .
X . X .
A 2, .
B 4, .
X Q-Score All Cycles — Q30 . Q-score . Q-score Cycle 25 .
Q-Score
Q40 1/10,000
Q30 1/1,000
. .
Q-Score
Q20 1/100
Q10 1/10
X Cluster Density(K/mm²) — . X Clusters Passing Filter(%) — Illumina Chastity Filter . Cycle 25 .

(chastity) . 25 0.6 .
X Estimated Yield(Mb) — . Cycle 25 .
2 Next . Sequencing .

Next Sequencing . Next Sequencing .
3 Next .
X Y . Real-Time Analysis(RTA) .
. 4 , .
(metrics) Sequencing . .
.

MiSeq Reagent Kits, v2
MiSeq Reagent Kits, v2
MiSeq Illumina Specifications for the MiSeq System (www.illumina.com/systems/ miseq/performance_specifications.ilmn) .
MiSeq
. .
RTA RTA (quality core) (*.bcl) . RTA 46 RTA .
(post-run wash) . . . 20 .
. . Perform Wash .

. .
.
X .
X .
X .
MCS v2.5 , (sodium hypochlorite, NaOCl) (template line wash) . 30 . 27 .

VeriSeq PGS . 27 .

X NaOCl( )
X MiSeq Tube( : MS-102-9999, )

1 Tween 20 .
a 45 ml 5 ml 100% Tween 20 . 10% Tween 20 .
b 475 ml 25 ml 10% Tween 20 . 0.5% Tween 20 .
c 5 .
2 .
a 6 ml .
b 500 ml 350 ml .
MiSeq
. .
3 Start Wash . Reagent Chiller . Post-Run Wash Perform optional template line wash . . 27 .
4 Reagent Chiller Reagent Chiller .
6 PR2 .
7 PR2 .

PR2 . PR2 .
8 . .

9 .
10 .
11 Next .
, , .

, . .
1 Tween 20 .
c 5 .
2 NaOCl .
a 864 μl 36 μl 5% NaOCl . 1:25 NaOCl .
MiSeq
. .

NaOCl . NaOCl . . 5% NaOCl 1 ml 0.01% NaOCl . NaOCl .
3 .
a 6 ml .
b 500 ml 350 ml .
4 17 0.01% NaOCl MiSeq Tube . . 17 Tween 20 .
19 17 MiSeq Tube

NaOCl MiSeq Tube 17 . MiSeq .
5 Start Wash . Reagent Chiller .
6 Post-Run Wash Perform optional template line wash . VeriSeq PGS Perform optional template line wash . MCS . Perform optional template line wash Run Review .
9 PR2 .
10 PR2 .

PR2 . PR2 .
MiSeq
. .
11 . .

12 .
13 .
14 Next .
, , .

, . .
MiSeq
. .
.....................................................................................................................................31
........................................................................................................................................33
Manage Files ....................................................................................................................................................35
............................................................................................................................................36
......................................................................................................................................................36
.

VeriSeq PGS VeriSeq PGS . 30 VeriSeq PGS .
2

Idle Mode(7 ) , idle 30

VeriSeq PGS VeriSeq PGS .
4

Perform Wash Idle Mode (3 )
Idle Mode(7 ) , idle 30

. .
5 Idle Mode (7 )

30 .
90 . 3 .
. 11 .

X
2 Home Perform Wash .
3 Perform Wash Perform Maintenance Wash . Reagent Chiller .
1 1 Tween 20 .
c 5 .
2 .
a 6 ml . b 500 ml 350 ml .
3 .
a Reagent Chiller Reagent Chiller .
b Reagent Chiller . Reagent Chiller .
c PR2 , PR2 .

PR2 . PR2 .
MiSeq
. .
a . .
b . c .
4 Next . 1 .
2 . .
1 Tween 20 .
c 5 .
2 1 .
3 .
a 6 ml . b 500 ml 350 ml .
4 .
a Reagent Chiller . Reagent Chiller .
b .
c .
5 Next . 2 .
3 1 Tween 20 .
c 5 .
2 2 .
3 .
a 6 ml . b 500 ml 350 ml .
4 .
MiSeq
. .
b .
c .
5 Next . 3 .
, , .

, . .
7 idle . idle 30 .
2 . , 2 . 60 .
Standby Mode Home . Standby Mode .

X
3 Wash Options Perform Standby Wash . Reagent Chiller .
1 1 Tween 20 .
c 5 .
2 .
a 6 ml . b 500 ml 350 ml .
MiSeq
. .
3 .
a Reagent Chiller Reagent Chiller .
b Reagent Chiller . Reagent Chiller .
c PR2 , PR2 .

PR2 . PR2 .
a . .
b .
c .
4 Next . 1 .
2 . .
1 Tween 20 .
c 5 .
2 1 .
3 .
a 6 ml . b 500 ml 350 ml .
4 .
b .
c .
5 Next . 2 .
, , .

, . .
MiSeq
. .
Manage Files Home Manage Files , , .
1 Manage Files Browse .
2 .
X . X Delete . Runs,
Sample Sheets, Manifests, Genomes Recipes .
3 Delete .
Delete Bundle Logs .
Move .
1 Manage Files Runs Browse .
2 .
3 Move .
4 Browse Network .
5 OK .
Upload Sample Sheets Genomes .
MiSeq USB .
1 Manage Files Sample Sheets Genomes Browse .
2 Upload .
3 Browse Network .
4 OK . Directory .
MiSeq
. .
1 Manage Files Sample Sheets .
X . X Delete .
2 Rename .
3 .
4 Next .
5 Back .
Home . BaseSpace . 12 BaseSpace .
.
Home Update Available . . MiSeq .
1 Home Update Available .
2 . . .
Manual Update MiSeq Control Software Analysis Software .
1 Main Menu Software Update .
2 Browse .
3 Save and Update .
4 . . .
, Windows .
1 . 31 .
2 . .
3 .
MiSeq
. .
4 Home Manage Instrument .
5 Shut Down . .
6 OFF .

ON 60 .
MiSeq
. .
A ....................................................................................................................................................................38
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RFID .....................................................................................................................................41
Local Run Manager .............................................................................................................43
...........................................................................................................................................43

Illumina . .
Illumina > MiSeq Support > FAQs MyIllumina Bulletins .
Illumina . 51 .
Illumina . Manage Files Bundle Logs . 38 .
Bundle Logs Illumina . Manage Files Bundle Logs (bundle) . zip .
Bundle Logs (bundle type) . Illumina Bundle Logs .
1 Manage Files Bundle Logs .
2 Browse MiSeqOutput .
3 Run QC Files, Thumbnail Images, Run Logs, Maintenance Logs .
4 Run .
5 Bundle Logs . Bundle Files .
Bundle Logs MiSeq Output and Analysis Folders Quick Reference Card( : 15034791) .
6 Next .
8 Save .
. .
Bundle Logs .
9 Illumina .
20 Bundle Logs
System Check Live Help Illumina . .
(volume test) Illumina . (volume) . 42 .

Tip/Tilt Full Optics Illumina .
1 Main Menu System Check .
2 .
X . X Select All .
3 Next . .
4 [ ] Show Details .
5 [ ] *.csv USB Export Results .
6 Done .
MiSeq , Sequencing .
MiSeq
. .
. , . .
Pause .
Sequencing Pause . Resume . Resume .
21 Sequencing
Sequencing Stop . .
. Real-Time Analysis .
22
. . .
MiSeq
. .
. .
2 Raise Sippers .
3 .
( ) . .

Free Disk Space . Manage Files .
Network Connection Active . Manage Instrutment Reboot . Manage Instrument Shut Down . 60 .
Primary Analysis Ready 1 . 1 1 , . 1 Terminate Analysis. 2 .
RFID RFID Illumina . 7.
1 RFID . RFID Enter Code .
2 my.illumina.com Sign In .
3 MyIllumina . Sign In .
4 Account . My Tools MiSeq Self-Service .
5 MiSeq Self-Service MiSeq serial number .
6 Type of Override Code RFID Override .
7 Get Code .
8 MCS Enter Code .
9 Next .
10 , PR2 .
MiSeq
. .

. A(Standard), G(Micro) D(Nano) . : A0E61
PR2 PR2 . : MS0011881-PR2
. : MS0010744-300
11 . Enter Reagent Kit Barcode .

.
12 Enter .
. .
(volume) . . .
1 .
2 Main Menu System Check .
3 Conduct Volume Test Next .
4 6 ml .
5 500 ml 350 ml .
6 .
a Reagent Chiller Reagent Chiller . Reagent Chiller .
b . .
c .
7 Next . . . 31 .
8 .
.
MiSeq
. .
1 .
2 .

17.25 ml
46 ml
51.75 ml
Reagent Chiller Reagent Chiller 2~11°C. Reagent Chiller . 6 .
Reagent Chiller Illumina .
Reagent Chiller 4 .
Local Run Manager Local Run Manager v3 ( : 1000000111492) . .
MCS . MiSeq .
IP DNS IP DNS .
X Obtain an IP address automatically Use the following IP address . Use the following IP address IP address, Subnet mask Default gateway .
X Obtain DNS address automatically Use the following DNS server address . Use the following DNS server addresses Preferred DNS server address Alternate DNS server address .
2 Save and Continue .
MiSeq
. .
B ................................................................................................................................................................44
MiSeqOutput ..................................................................................................................................44
RTA .................................................................................................................................................46
MiSeq .
X D:\Illumina\MiSeqTemp — , MCS RTA . MiSeqTemp . 7 .
X D:\Illumina\MiSeqOutput — RTA MiSeqTemp MiSeqOutput . RTA 1 MiSeqOutput MiSeqAnalysis . MiSeqOutput . 46 RTA .
X Run Options Output Folder . 12 .
X D:\Illumina\MiSeqAnalysis — RTA Local Run Manager MiSeqAnalysis 2 . MiSeqAnalysis MiSeqOutput . 44 MiSeqOutput .
BaseSpace MiSeqAnalysis .
, .
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1 .
MiSeqOutput RTA MiSeqOutput 2 . 2 MiSeqOutput MiSeqAnalysis . , MiSeqOutput Images Thumbnail_Images . 2 .

Output Analysis .
X SampleSheet.csv — . SampleSheet.csv . Data\Intensities and Data\Intensities\BaseCalls .
X runParameters.xml — RFID .
X RunInfo.xml — .
: 15027617 v06 KOR : 20000262 44
. .

Output Analysis .
X <Run folder name>\Config — .
X <Run folder name>\Data — Intensities, BaseCalls Alignment . Local Run Manager Alignment .
X <Run folder name>\Data\RTA Logs — RTA .
X <Run folder name>\Data\Intensities\BaseCalls — (*.bcl) , (matrix) (phasing) . 2 Local Run Manager FASTQ . Local Run Manager v3 ( : 1000000111492) .
X <Run folder name>\Recipe — .
X <Run folder name>\Logs — .
X <Run folder name>\InterOp — , , 1 Sequencing Analysis Viewer(SAV) .
Output Analysis . .
Local Run Manager 2 Alignment . Local Run Manager v3 ( : 1000000111492) .
MiSeq
. .
RTA 1 Real-Time Analysis(RTA) . Local Run Manager 2 .

RTAComplete.txt . 2 .
SampleSheet.csv 2 .
RunInfo.xml ( ) .
*.bcl Data\
Intensities\BaseCalls\
L001\CX.X
*.bcl 1 , 1 RTA .
*.stats Data\
Intensities\BaseCalls\
L001\CX.X
/ RTA *.stats .
*.filter Data\
*.txt Data\RTALogs 1 .
*.cif Data\
Intensities\L001\CX.X
*.cif 1 , 1 RTA . 47 .
*.locs Data\
. *.locs 1 .
*.jpg Thumbnail_Images\
L001\CX.X
, . Analysis . 47 .
. MiSeq .
MiSeq Reagent Kit
Standard Flow Cell MiSeq Reagent Kits, v3 19 38
PGS Flow Cell MiSeq Reagent Kit v3-PGS 19 38
Standard Flow Cell MiSeq Reagent Kits, v2 14 28
Micro Flow Cell MiSeq Reagent Micro Kits, v2 4 8
Nano Flow Cell MiSeq Reagent Nano Kits, v2 2 2
1 Output . 47 .
MiSeq
. .
1 Output , 4 . Nano Flow Cell . Output Data\ Intensities\BaseCalls\L001 .
MiSeq Reagent Kit
Standard Flow Cell
PGS Flow Cell
~
2119
~
2114
~
2104
~
. .

6 51 1, 51 1 3 3, 4 9 8, 46 3~4
idle 33 36 36 51 6

24 17 14 40 40
1 46 Temp, Output, Analysis 44 35 44 44 5
35 15

31
Bundle Logs 38 RFID 41 38 42

35 46 5
6 13 13

8 14 36
33 43
8
PR2 21 22
3 9 10 15 16
15 4 13

. .
6
Idle 33 36 31 33 42 26 26 11
42
42 31, 33
12

47 26 7, 25

35 35 35 4 26 12 23
8 8 19 9 46 47
3~4 4

B
Bundle Logs 35
I
Illumina Proactive 12 InterOp 45 IP 43
L
M
MiSeq Self-Service 41
R
Reagent Chiller 6 Real-Time Analysis 1, 5 46 44 25
RFID PR2 21 41 22 1
RTAComplete.txt 46
. .
RunInfo.xml 44, 46 Run Options 11~12 runParameters.xml 44
S
Sequencing Analysis Viewer 7, 24 Sequencing 24 Software Suite 5 Software Update 12 Support 1
V
VeriSeq PGS 30 8
MiSeq
. .

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RFID
Local Run Manager

Documents

MiSeq 시스템 가이드 - Illumina, Inc. · 2021. 1. 7. · MiSeq Reporter를 다시 추가하고 Local Run Manager 관련 내용 삭제. 문서의 제목을 'MiSeq 시퀀싱 시스템