เนื้อเยื่อพืชPLANT TISSUES

Dr. Boonsatien Boonsoong

Department of ZoologyFaculty of Science

Kasetsart University

Objectives:

ศึกษาเปรียบเทียบลกัษณะที่แตกตางกันของพืชใบเลี้ยงเดี่ยวและพืชใบเลี้ยงคู

ศึกษาตําแหนงและการจัดเรียงตัวของเนื้อเยื่อและเซลลแตละชนิดในพืช

ศึกษาเนื้อเยื่อชนิดตางๆ ของพืชPLANT TISSUES

ฝกการเตรียมสไลดสดโดยวิธี free hand section

Plant growth:

primary tissue

• epidermis• cortex• primary xylem• primary phloem

vascular cambium

• secondary xylem• secondary phloem

secondary growthprimary growth

apical meristem lateral meristem

differentiate

cork cambium

periderm(cork, phelloderm)

differentiate differentiate

Stem growth:

Plant growth: Plant tissues: cell division

• Meristematic tissue

• Permanent tissue

• Apical meristem

• Lateral meristem: vascular cambium, cork cambium

• Intercalary meristem

• Simple permanent tissue: epidermis, cork, parenchyma, collenchyma, sclerenchyma

• Complex permanent tissue: xylem, phloem

Meristematic tissue:

Apical meristem

shoot apex root apex

Meristematic tissue:

Apical meristem

• Vascular cambium - สราง secondary xylem และ secondary phloem - Dicot

Meristematic tissue:

Lateral meristem

Meristematic tissue:

Lateral meristem (cont.)

• Cork cambium - สราง cork และ phelloderm

Meristematic tissue:

Intercalary meristem

Permanent tissue:

Cork

Parenchyma

Collenchyma

Sclerenchyma

Simple

Epidermis

dermal tissue, single layer of cells

• secretes a waxy layer called the cuticle that inhibits water loss

Simple permanent tissue:

Epidermis

guard cell

Simple permanent tissue:

Epidermis

non-living, water-resistant• protective tissue that displaces the stem epidermis

Simple permanent tissue:

Cork• healing and tissue regeneration• photosyntesis

Simple permanent tissue:

Parenchyma

• food storage

ChlorenchymaParenchyma

intercellularair space

Simple permanent tissue:

Parenchyma

palisade cell

spongy cell

Transverse section of leaf of common barberry (Berberis vulgaris)

• unevenly thick primary cell wall• support in young stems, roots, and petrioles

Simple permanent tissue:

Collenchyma

Transverse section of stem of angelica

Simple permanent tissue:

Collenchyma cells

Celery (Apium)

C.S.

L.S.

• dead at maturity• support and protection

Simple permanent tissue:

Sclerenchyma

• secondary cell wall

X-section L-section

Simple permanent tissue:

Sclerenchyma

Fiber Sclereid

Simple permanent tissue:

Sclereid

Pear (Pyrus)

Permanent tissue:

Complex

Cork

Parenchyma

Collenchyma

Sclerenchyma

Xylem

Phloem

Simple

Epidermis

Complex permanent tissue:

Vessel member

Tracheid

Xylem fiber

XYLEMXYLEM• Conduct waterand dissolvedminerals

• Support

Xylem parenchyma

Xylem:

Vessel Tracheid

• dead at maturity• end wall - perforation

• end wall - imperforation

• angiosperm• gymnosperm

Complex permanent tissue:

Phloem fiber

Sieve tubemember

Companioncell

• conduct food and other organic substances

Phloemparenchyma

PHLOEMPHLOEM

Phloem:

Sieve tube Companion cell

• sieve plate

Monocot & Dicot:

Monocot & Dicot:

Monocot stem, c.s. Dicot stem, c.s.

Stem monocot:

X - section

Stem monocot:

Vascular tissue

X - section

Stem dicot:

X - section

Epidermis

Cortex

Vascular bundle

Pith

Stem dicot: Stem dicot:

Epidermis

Cortex

Phloem

Pith

Xylem

Stem dicot:

Vascular tissue

X - section

Lab. session:

Meristem tissue

• L-S), Allium sp. root

• X-S, Coleussp. stem

Permanent tissue

• Epidermis

• Periderm

• Collenchyma

• Sclerenchyma

• Parenchyma

• Fiber

• Chlorenchyma

Monocot & Dicot

• Compare

• X-S, corn stem

• X-S, Coleus sp. stem

• Vascular bundle

Permanent Slide

Free hand sectioning:

Wet mount

• X-S, หญาละออง

• X-S, วานฝกดาบ

• X-S, ฝรั่ง

• กะลามะพราว• Safranin dye

• Aniline sulfate

Lab. results:

Fiber ของวานฝกดาบ

Safranin stained Aniline sulfate stained

Lab. results:

X-S หญาละออง

Vascular tissue, safranin stained

Lab. results:

Sclerenchyma ของฝรั่ง

Sclereid, safranin stained