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Quy trnh v cc giai on ln men ru:
1.ng ha
Hnh 1Qu trnh ng ha
-Ngun enzyme:
+Thc vt:
Malt c -amylase, -amylase, limit dextrinase,
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r-enzyme
Cc enzyme ny hot ng cng lc v rt nhanh
ng ha khng hon ton nn cn phi ln men+Vi sinh vt:
Bacillus licheniformis
Bacillus subtili
Aspergillus oryzae
Cc vi sinh vt ny tit ra enzyme - amylase
- Cc phng php ng ha:Bngacid
Menthucbc
Maltase
Amylo Myco-malt
Tcnhn
ngha
HCl(810 %)
H2 SO4(25%)
Nmmc
tnhientrongthucbc
-Malt-
ccloithocnymm
Amylomyces
rouxii,MucorhayRhizopuscytrctip
Enzymeamylase
ca- Asp.Oryzae- Asp. Niger- Asp.Awamori
2. Qu trnh ln men:
Phng trnh tng qut ca qu trnh ln men:C6H12 O6 = 2C2H5 OH + 2 CO2
+ 2ATP
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- S ln men glucose thnh ethanol v CO2 nhsaccharomyces cerevisiae
din ra theo com ng EmbdenMeyerhof. S chuyn ha pyruvate thnh ethanol
gm hai
bc. Trong bc mt, pyruvate c loi CO2 thnhacetaldenhyde nh
pyruvate decarboxylase vi s tham giaca tiamnpirophosphate. Trong bc
sau, acetaldehyde b khthnh etanol nh alcohol dedihydrogenase cha
NADH.
Hnh 2 s ln men ethanol nh nm men
Bng s chuyn ha H ny, dihydro tch ra do triosephosphate
dehydrogenase xc tc sc tiu th, nh vy trng thioxi ha kh sc cn
bng.
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3. Chng ct, tinh ch:- Mc ch:+Thu c ru c cn cao hn+Loi b mt s tp
cht
- 2 giai on:
+ Chng ct th: tch cn cng cc cht dbay hi rakhi dch ln men+ Tinh
ch: loi tp cht ra khi cn th thu ccn tinh khit
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Hnh 4 s chng ct ru
Phn loi tp cht (da vo trng lng phn t,khnng bay hi): Tp cht u (ru
u, ru kngh)
-Aldehyde, acetic, ethyl acetate, methyl acetate, ru metanol- si
thp hn ru etanol-c ly ra giai on u ca qu trnh tinh ch Tp cht cui
(du fusel hay ru tp):- Ru cao phn t: isoamylic, isobutylic- nhit si
cao hn etanol , kh bay hi, t hoa tan trongnc Tp cht trung gian:
- Ethyl isobutyrate, ethyl isovalerianate.- Tuy thuc, nng ru va
tinh cht vt ly ca tp cht
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Tch nc:
c im: hn hp ru nc l hn hp ng ph (c imsi chung)
Phng th nghim Trong sn xut lnCht ha nc nh CaCO3,KCL, CuSO4,
-Chng luyn p sut thp(P = 0,0525 atm)- Ru nc khng c imsi
chung
Hiu sut thu hi cn thp (60-65% so vi ru th ban u
Thu c cn tuyt i
