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1396 "#$% &’ ()%* +$ ,- (Rosmarinus officinalis) ).$ /* (0, 12 ) 3#$ (Hypophthalmichthys molitrix) 4 ,4 4 164 (%$ 4)7 )2, &8, 1 * &$9: )0- 2 (* -, 3 1< 4 : 95 : 95 &= !"#$ %&$ ’ ( )*+ ,$ - ./* .1 $ )23 45 6 7- 8&9 : ; 4 9< 6 =&1 $ ( & 8 : / : -$ 6 >?& @< . AB& $ .(6&( >$ - D) - : ;$ )23$ / >F91 $ -: ) 5 / 1 ( (+ ) /:8& 5 / 0 4 J 61 K*$ ( 1 $ ( /:8& L:< ( (A%:< A% 69< ( / >$ . 9< Salmonella typhimurium : ( 9: 6-$ - M:$ N log CFU/g 5 O:? :J /:*P- . 9< -: Q - % 9< .;< - $1 - % < . 9 - 9< ( Q:9<! :1 S. typhimurium M$ ) R9S$ - 0 . 3 . 6 ( 9 V9& . 6 (( 6<5 >$ 1 6*)$ WX$ %J ) 05 / 0 < P ( :D 6Y$ -: Q - - Z . B$ >F91 L$ [\ /:8& ( $ 6&( >$ ( Q - 9 ?$ 8 9 . ] 6W:< 25 - . 6$ -: M ( $ B$ & /:8 M*\ . A& N9: 6-$ : 6([:$ $ M ^:5 >% -: 7 :&2* 4 .$ =&1 ./:8& .69< >?& @< 6-$ . 1 - 91 >‘Y& . 5( .a: >(% M . .6$a1 . 2 - 91 M . .6$a1 >‘Y& . 5( .a: >(% . 3 - Z*< a: . c\ >‘ >‘Y& . 5( .M‘WS& ( M M‘Y-(d .6$a1 . .M 4 - Z*< ( A\ .6 f*+ . .6$a1 >‘Y& . 5( M . * :gh8$ >*8& [email protected]

Rosmarinus officinalis ... file1396 !(1)5 : ˘ˇ ˆ ˙ ˝ ˛ ˘ˇ ˆ ˛ [88] 1,&., 6˝ -˝ ˘ˇˆ ˙˝ y< ( ˛˚˜ )1

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Page 1: Rosmarinus officinalis ... file1396 !(1)5 : ˘ˇ ˆ ˙ ˝ ˛ ˘ˇ ˆ ˛ [88] 1,&., 6˝ -˝ ˘ˇˆ ˙˝ y< ( ˛˚˜ )1

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!�"#$�F�� �*< )Yin and Cheng,

2003.( � ��91�� l?#� /�� 7��-�.

�(���- �� �< �&�� >�F91� �:& 6��:�:�

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aromaticum( ���$�� ( )Rosmarinus

officinalis( >�����< .M���[�- ( ��[��n)

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,8:&�%��(�[:$ ,<���6���� �8� LW8$ ��-.

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al., 2008; Shahbazi, 2016.( >�(�$� >�F91�

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Page 3: Rosmarinus officinalis ... file1396 !(1)5 : ˘ˇ ˆ ˙ ˝ ˛ ˘ˇ ˆ ˛ [88] 1,&., 6˝ -˝ ˘ˇˆ ˙˝ y< ( ˛˚˜ )1

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( �� ���4���:� �(� �� �� ��@1� 6-�$ ��-

)Ghomi et al., 2011.(

Page 4: Rosmarinus officinalis ... file1396 !(1)5 : ˘ˇ ˆ ˙ ˝ ˛ ˘ˇ ˆ ˛ [88] 1,&., 6˝ -˝ ˘ˇˆ ˙˝ y< ( ˛˚˜ )1

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Page 5: Rosmarinus officinalis ... file1396 !(1)5 : ˘ˇ ˆ ˙ ˝ ˛ ˘ˇ ˆ ˛ [88] 1,&., 6˝ -˝ ˘ˇˆ ˙˝ y< ( ˛˚˜ )1

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Arashisar S., Hisar O., Kaya M.

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Aquatic Physiology and Biotechnology

Vol. 5, No. 1, Spring 2017

Antibacterial potential of rosemary (Rosmarinus officinalis) essence and nisin on silver carps

(Hypophthalmichthys molitrix) fillet at temperature of 4ºC

Mahshid Shamloofar1* , Zahra Ghiasvand2, Maziar Kamali 3, Fatemeh Bai4

Received: September 2016 Accepted: January 2017

Abstract Enhancing the survival of the aquatic products is one of the most important

concerns during processing industry. So the aim of present study was evaluating the antibacterial effects of rosemary essence and nisin on silver carp’s fillet. The treatments had composed of rosemary (1.5%), nisin (0.5g/Kg), both nisin and rosemary and control group without any additive. In order to evaluate the antibacterial efficiency of rosemary and nisin, the Salmonella typhimurium (as an index pathogenic bacteria, 5 log CFU/g) was inoculated on silver carp’s fillet and the growth of the bacteria was measured every three days (0, 3, 6, 9). The results revealed that the significant positive effect has been observed in all treatments in comparison with the control group (P<0.05). The independent usage of nisin and rosemary had better effects than the combined treatment. According to the results, we can use nisin and rosemary separately as a selected treatment for inhibiting the growth of bacteria on silver carp’s fillets during the preservation.

Key words: Antibacterial, Nisin, Salmonella typhimurium, Silver Carp. 1- Assistant Professor in Department of Fisheries, Azadshahr Branch, Islamic Azad University, Azadshahr, Iran. 2- Assistant Professor in Department of Fisheries, Azadshahr Branch, Islamic Azad University, Azadshahr, Iran. 3- M.Sc. in Fisheries, Member of Young Researchers and Elite Club, Azadshahr Branch, Islamic Azad University, Azadshahr, Iran. 4- M.Sc. in Food Industry and Science, Azadshahr Branch, Islamic Azad University, Azadshahr, Iran. *Corresponding Author: [email protected]