CHNG IIILN MEN CC SN PHM T SINH KHI T BO VI SINH VT SN XUT PROTEIN N BO

MC TIUNm c u v nhc im ca protein n bo t vi sinh vtNm c cc vi sinh vt tham gia vo qu trnh sn xut protein n bo.Hiu r Quy trnh chung sn xut protein n bo.

NI DUNG1.u v nhc im ca protein t vi sinh vt.2. Cc yu cu c bn ca vic sn xut protein n bo.3. Vi sinh vt dng trong sn xut protein n bo.4. Quy trnh sn xut protein n bo t vi sinh vt.

SN XUT PROTEIN N BO1. u v nhc im ca sn xut protein t vi sinh vt1.1. u im:+ t tn din tch+ Tc sinh trng cao: gp 100 1000 ln so vi i gia sc + Khng ph thuc vo kh hu+ Thnh phn v gi tr dinh dng ca sinh khi c th iu chnh c bng cch thay i thnh phn mi trng, iu kin nui cy hoc to ging mi+S dng nguyn liu sn c v r tin.

1.2. Nhc im+Trong sinh khi ca vi sinh vt cha nhiu axit nucleic (10-20%) khng c li cho sc khe ca con ngi. + Protein vi sinh vt c hng v cha cao + Cn phi nm bt c k thut, thit b nui cy vi sinh vt+ Kh p dng sn xut quy m nh

2. Cc yu cu c bn ca vic sn xut protein n bo2.1. S dng nguyn liu r tin2.2. Tc sinh trng cao2.3. Hm lng protein cao2.4. Cht lng protein cao2.5. Kh nng tiu ha ca protein2.6. S an ton v c t2.7 + Vi sinh vt phi d tch, d x l. + Tnh khng mn cm vi s tp nhim.

* Cc gii php k thut lm gim lng acid nucleic Gim mnh tc sinh trng. Chit rt ARN bng NaOH 10% nng. Thy phn ARN bng kim v tch protein ha tan trong bng kt ta Phn hy ARN bi enzym nucleaza a vo hoc ca bn thn t bo.

3.Vi sinh vt dng trong sn xut protein n bo3.1. Nm men:Nm men giu protein (40 60%)Giu vitamin (nht l vitamin nhm B) S dng rng ri nht l Candida, Torulopsis, Saccharomyces

3.2. Nm si: Hm lng protein thp (30%)Rt d tch sinh khi v to hng v c bit.trong thc phm ngi ta s dng cc loi Morchella v cc hn hp ging

3.3 Vi khun Vi khun dng sn xut protein n bo thng c nui cy trn cacbuahydro + Thng s dng cc ging Pseudomonas, Flavobacterium, Mycobacteriuum, Nocardia.+ i vi nguyn liu l metan ngi ta thng s dng Methylomonas methania, Methylococens capsulatus

3.4. Vi khun lam v vi to Hm lng protein chim khong 40 - 60%Hm lng aminoaxit ca hai loi ny kh cn iCha nhiu vitamin: A, B, K, C, B12Trong sinh khi ca vi khun lam cn c cha khng sinh nn bo qun tt.

4. Quy trnh sn xut protein n bo t vi sinh vtNguyn liuchun b mi trng dinh dng

X l mi trng

Vi sinh vt thun khit Ln men tch sinh khi x l Hon thin Sn phm

* Nguyn liu: + R ng+ Nc thi ca nh my sa+ Tinh bt, dch g thy phn, dextrin, cacbuahydro* X l:+ Lm sch+ Nghin nh+ Thy phn*Ngun dinh dng b sung:+ N, P, k,+ Cc nguyn t vi lng v kch thich sinh trng (cao ng, cao nm men.+ Cc cht iu chnh pH: NH4+, NaOH.

*Kh trng mi trngThit b tit trng lin tc YHC 1-Thng cha; 2-Bm; 3- B un nng; 4- B gi; 5- B ly mu; 6- Thit b trao i nhit- thu hi; 7- Thit b trao i nhit- thit b lm mt; 8- Thit b ln men.

* Cng tc chun b ging+ Cy ging trong phng th nghim

S nui cy ging.1.Thng gy men 150 l cp 1 cha 100 l dch; 2.Thng ng hathm v x l dich ng; 3 v 4. Hai thng gy men cp II c dungtch bng dung tch thng ng ha thm v u bng 10% so vi thng ln men.+ Nhn ging trong sn xut

* Cc phng php ln men+ Ln men gin on:Thng ln men gin on.1.rut g lm lnh cn 0,4-0,5m2/m3 thng; 2.ng dn dch ng v men ging; 3.ng tho gim chn; 4.ng thot CO2; 5.Ca quan st v v sinh; 6.u ng ni h thng v sinh 7 vi pha trong thng; 8. Van ly mu; 9. u ng ni h thng sc kh hoc CO2 v hi thanh trng.

+ Ln men lin tcS ln men lin tc

+ Ln men ci tin (bn lin tc)S ln men bn lin tc

* Tch sinh khi+ Lc+ Lng+ Ly tm*X l sinh khi+ Ra+ Chit rt protein (Loi bt ARN sy)

CU HIBi 1: trong thc t sn xut protein n bo ngi ta thng s dng loi vi sinh vt no? Phn tch ly v d?Bi 2: Bn hy phn tch yu cu v chng vi sinh vt sn xut protein n bo?

TI LIU THAM KHOTrn Th Thanh. Cng ngh vi sinh. Ti bn ln th ba. Nh xut bn Gio dc.Nguyn Xun Thnh, Nguyn B Hin, Hong Hi, V Th Hoan. Gio trnh vi sinh vt hc cng nghip. Nh xut bn Gio dc.Nguyn Ln Dng, Nguyn nh Quyn, Phm Vn Ty. Vi sinh vt hc. Nh xut bn Gio dc.Lng c Phm. Nm men cng nghip. Nh xut bn Khoa hc v K thut.Cc webside