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UNIT 6: PHOTOSYNTHESIS
(PROCESS OF FOOD
PRODUCTION BY PLANTS)
WHAT IS PHOTOSYNTHESIS? The process that occurs in green plants, whereby solar energy is converted into chemical energy and stored as organic molecules by making use of carbon dioxide, sunlight, and water. Water and Oxygen are formed as byproducts
Photosynthesis can be summarized in the following equation:
6 CO2 + 12 H2O + Light energy
C6H12O6 + 6 O2 + 6 H2O (glucose)
WHO OR WHAT CAN PHOTOSYNTHESIZE?
Photoautotrophs can photosynthesize.
Include: Green plants, algae, cyanobacteria and green protists.Plants
Green protists
Cyanobacteria
Algae
WHY DO PLANTS PHOTOSYNTHESIZE?
To provide nutrients and oxygen for heterotrophs.
Heterotrophs are dependent on autotrophs, because they cannot produce there own food.
WHAT PART OF THE PLANT IS RESPONSIBLE FOR PHOTOSYNTHESIS?
Photosynthesis occurs in the chloroplasts of plant cells.
The chloroplasts are mainly concentrated in the mesophyll cells of leaves.
Chloroplast contain chlorophyll – green pigment that absorbs sunlight.
Chlorophyll fill the space in the thylakoid membrane.
CHLOROPLAST MESOPHYLL CELL (PALLISADE AND
SPONGY)
1 µm
Thylakoidspace
Chloroplast
GranumIntermembranespace
Innermembrane
Outermembrane
Stroma
Thylakoid
RAW MATERIALS OF PHOTOSYNTHESIS
• The raw materials of photosynthesis are:
water, carbon dioxide and sunlight.
HOW RAW MATERIALS REACH THE CHLOROPLASTS
Water is absorbed through the root hair into the xylem of the roots, into the xylem of the stem, through the xylem of the leaves into the mesophyll cells and finally into the chloroplasts.
Carbon dioxide diffuses from the atmosphere through the stomata, into the intercellular airspaces in the leaves, and finally into the chloroplasts of the mesophyll cells.
The chlorophyll and other pigments in the thylakoid membrane absorb the solar energy to drive photosynthesis
PHOTOSYNTHESIS CONSIST OF 2 STAGES:
LIGHT REACTION PHASE (Dependent on light)
DARK PHASE/ CALVIN CYCLE (Light independent)
LIGHT REACTION PHASE Takes place in the thylakoids of the chloroplasts.
Chlorophyll absorbs solar energy from the sun.
When a chlorophyll pigment absorbs light energy, it excites the electrons, which goes from ground state to an excited state, which is unstable, but can be used as potential energy.
When unused excited e- fall back to the ground state, and heat are given off.
The electrons are excited in the photosystems fount in the thylakoid membrane
This potential energy is then used firstly to split water – into hydrogen & oxygen.
2H2O 2H2 + O2
Oxygen is released as a byproduct – diffuse through stomata into atmosphere.
The hydrogen reduces NADP+ to NADPH Some energy is then used to
photophosphorylate ADP to generate ATP. ADP + P
ATP
[Light
Fig. 10-5-2
H2O
Chloroplast
LightReactions
NADP+
P
ADP
i+
ATP
NADPH
O2
CALVIN CYCLE Carbon dioxide diffuses through the
stomata of the leave and finally into the stroma of the chloroplast.
The carbon dioxide is accepted by a 5C molecule called ribulose biphosphate (RuBP) which then forms an unstable 6C compound.
6C compound dissociates into 2 x 3C compounds called phosphoglycerate (PGA)
PGA is then reduced to phosphoglyceraldehyde (PGAL/ G3P) by accepting a phosphate from ATP and a hydrogen electron from NADPH.
Thus changing ATP back to ADP and NADPH to NADP.
PGAL are now used for the following reactions: Some PGAL are used to make RuBP again, so that the cycle can start over again.
Some PGAL are used to form hexose sugars like glucose and fructose. Which combine to form disaccharides and polysaccharides.
* The carbohydrates can then be converted to other biological compounds like proteins or fats by adding mineral salts like nitrates and phoshates.
CO2 +
RuBP(5C)
6C compound
2x PGA (3C)
PGALATP = ADP + P
NADPH = NADP + H
.
Fig. 10-21
LightReactions:
Photosystem II Electron transport chain
Photosystem I Electron transport chain
CO2
NADP+
ADPP i+
RuBP 3-Phosphoglycerate
CalvinCycle
G3PATP
NADPHStarch(storage)
Sucrose (export)
Chloroplast
Light
H2O
O2
THE NATURE OF SUNLIGHT
Light is a form of energy = ELECTROMAGNETIC ENERGY/ ELECTROMAGNETIC RADIATION
The electromagnetic energy travel in waves. Distance between crests of electromagnetic
waves = WAVELENGTH Wavelength range from ≤ 1nm (gamma rays)
– ≥ 1 km (radio waves) The entire range of radiation wavelengths =
ELECTROMAGNETIC SPECTRUM
ELECTROMAGNETIC SPECTRUM
UV
Fig. 10-6
Visible light
InfraredMicro-waves
RadiowavesX-raysGamma
rays
103 m1 m
(109 nm)106 nm103 nm1 nm10–3 nm10–5 nm
380 450 500 550 600 650 700 750 nm
Longer wavelength
Lower energyHigher energy
Shorter wavelength
The most important part for life is the visible light (380nm – 750nm)
We can see this light as various colours. Light consist of particles = PHOTONS Photons have energy- The shorter the wave
length the greater the energy of the photon.
Therefore violet light has more energy than red light.
Photosynthesis are driven by visible light of the sun.
MAIN PIGMENTS USED DURING PHOTOSYNTHESIS:
Chlorophyll a – Absorb violet, blue and red light. Reflects and transmits green light (that is why plant leaves appear green)
Chlorophyll b – Absorb violet, blue and red light. Reflects and transmits green light (that is why plant leaves appear green).
Carotenoids – Play an accessory role in photosynthesis. They are shades of yellow and orange and able to absorb light in the violet-blue-green range. These pigments become noticeable in the fall when chlorophyll breaks down.
HOW A PHOTOSYSTEM HARVESTS LIGHT
The thylakoid membrane of a chroloplast contains several photosystems.
A photosystem consist of a protein complex called a reaction-centre complex surrounded by several light harvesting complexes.
Study the diagram to understand the process of light harvesting.
THYLAKOID SPACE(INTERIOR OF THYLAKOID)
STROMA
e–
Pigmentmolecules
Photon
Transferof energy
Special pair ofchlorophyll amolecules
Th
yla
koid
me
mb
ran
e
Photosystem
Primaryelectronacceptor
Reaction-centercomplex
Light-harvestingcomplexes
The Importance of Photosynthesis: A Review
Energy entering chloroplasts as sunlight gets stored as chemical energy in organic compounds
Sugar made in the chloroplasts supplies chemical energy and carbon skeletons to synthesize the organic molecules of cells.
Plants store excess sugar as starch in structures such as roots, tubers, seeds, and fruits
In addition to food production, photosynthesis produces the O2 in our atmosphere