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: 5- 3-
Replicare (.) - Escherichia coli 30,000 / 3,000 /
10-9
160 80 , 14 , , 5*1012 25*106
E. coli
?
9- DnaA DnaB 13- SSB
-
- , 10*103 rpm
?
:~1000 bp E.coli~100 bp
Proofreading
? :)
DNA-polymerase I
1959 ( .)
polA ! ?
E.coli 5 -
I
I , Fig 5-3 Chemistry of DNA Replication
Spontaneous reversal
II
(3)
II. II . II :(1) , (2) ,(3) .
H
HHL
L
L
HL
L
H
LH
new H2 : H L . - 3 OH
L
L
174(+)
(RF):
(+)(+)(+) (+)-
6,5*107 (..) 2,6*103 ../ 8,5 , 3-4 . 7000
5- , N-
29
-dCMP-OH 5-
-
/: 3- 5- (leading/lagging) ( )
---
Das Schluss
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