,

As easy as pie

: 5- 3-

Replicare (.) - Escherichia coli 30,000 / 3,000 /

10-9

160 80 , 14 , , 5*1012 25*106

E. coli

?

9- DnaA DnaB 13- SSB

-

- , 10*103 rpm

?

:~1000 bp E.coli~100 bp

Proofreading

? :)

DNA-polymerase I

1959 ( .)

polA ! ?

E.coli 5 -

I

I , Fig 5-3 Chemistry of DNA Replication

Spontaneous reversal

II

(3)

II. II . II :(1) , (2) ,(3) .

H

HHL

L

L

HL

L

H

LH

new H2 : H L . - 3 OH

L

L

174(+)

(RF):

(+)(+)(+) (+)-

6,5*107 (..) 2,6*103 ../ 8,5 , 3-4 . 7000

5- , N-

29

-dCMP-OH 5-

-

/: 3- 5- (leading/lagging) ( )

---

Das Schluss