1 2 3

1. 2. 3. 4.Ig 5.VC 6. 7.

1. antibody, Ab 2. immunoglobulin, Ig

BCRB) membrane immunoglobulin, mIg secreted immunoglobulin, sIg

Y 5: 2: V HVR3 CDR : FR4 C

VLCLVH CH1CH2CH3CH4 IgG IgM IgA IgE IgD

1. VLVHFV2. CLCH3. IgGCH2IgMCH3C1q4. CH3/CH4BNKFc5. IgGCH2+CH3 IgG

Immunoglobulin Fragments: Structure/Function Relationships

1. Papain Fabfragment of antigen- binding Fab Fcfragment crystalizable FcIg

2. Pepsin F( ab )2 2 pFc

3. Ig

1. joining chainJIgMIgA 2. secretory piece, SPSIgA

isotype IgC

1.H IgG (gamma) IgA (alpha) IgM (mu) IgD (delta) IgE (epsilon) (1) (2)IgGIgG1, IgG2, IgG3, IgG4 IgAIgA1, IgA2

2.(C) (1) (kappa) (lambda)(2)() OZ(+) (1) 190() OZ(-) (2) 190() Kern(+)(3) 154() Kern(-)(4) 154()

allotype CHCL (genetic markers ) GmGm130 AmA2m1A2m2 KmKm1Km2Km3

idiotype BVTBV

PH

Ag 1.2.

AbIgMIgG+ Ag C1q IgG4IgA IgE Fc (1) (2) opsonizationIgG + () FcR (3) ADCCIgG + () Fc RNK

Fc() IgGFcR FcR IgG(IgG1IgG3)FcR IgGFcRIgG1IgG3 NK IgGNK ADCC

() IgEFcFcRIg, IgE FcR(CD23), C B

() IgAFc FcR(CD89)IgADCC() IgMIgDBB

FcRFcRFcR

IgDNAIgVIg C(Immunoglobulin superfamily, IGSF)

1. TB 2. MHC 3. Ig 4. 5. 6. CD

IgG 1. (1) (2) (3)(75%Ig) (4)(2123) (5)35(8.017mg/ml) (6)SPA

2.(1)(2)(3)(4)ADCC3.(1)(2) (3)()(4) (5) IgG (6)

IgM 1.(900kd) 2.Ig 3. Ig 4.Ig510% 5.5 IgM 6. 7.IgM IgM 8.BIgMmIgM 9.

IgA 1.

2.6 3.Ig515% 4. 5.IgA IgA1

IgA2,

Origin of Secretory Component of sIgA

IgD 1. 2. B, 3. 1B 2B 4.Ig1% 5.3

IgE 1. 2.(Ig0.002%) 3. 4. 5. 6. IgE

1

2

1VDJ2V-D-JV-J3VDJN4LH5

class switch Igisotype switchBIgM, IgG

B V-D-JC IgM CD40/CD40L C Ig

TD-Ag

B

polyclonal antibody 1. BBpolycolonal antibody, PcAb 2. 1 2

monoclonal antibody, McAb 1. B 2. 3. B 4. HAT(H)(A) (T)

PCR -chimeric antibody reshaped humanized antibody bispecific antibody

1.2.3. 4.5.

CDR and FR

VHVL

IgG

FcADCC

Study GuideCan you describe the major regions of an immunoglobulin molecule?What distinguishes the immunoglobulin classes from one another?What is shared between classes?What is the significance of the variable regions?What is the significance of the Fab, the Fc, disulfide linkages?450-55050-70KD521425KDIgN1/41/2 AA VL VH3HVR1~3) Ig complementarity- determining region, CD1~3) 4framework region, FR1~4). AA C3/41/2 AACCHCLCLIgCHCH13CH14Ig CFcADCCI3025CH1/CH2JIgIgIgAJIgAIgASIgAIgAIgA