174 2017 年3 月12 日受理 * 豊田理研スカラー北海道大学大学院工学研究院応用化学部門 3 次元アミロイド構造体を利用した免疫測定チップの開発 Amyloid fibrils have attracted attention as one of the new nanobiomaterials. The amyloid fibrils show great features for biomaterial-based applications such as self-assembly, high thermal stability, and high Young’s modulus. However, the growth control of the amyloid fibrils is not well understood in detail. In this study, we demonstrated the methodology for growth control of the amyloid fibrils using a microfluidic device. The 3D amyloid-based structures were applied to a support for immobilization of enzyme. The enzyme immobilized 3D amyloid-based structures enabled the effective reaction compared with the free enzymes. １．はじめに flı°†’^Qﾏs^ì¦DÅ¼yｵ"ｩｰs})4m‹«ｯｭf¯±Æ„ﾊÖカÉya« ザ(ジ _flı°†’R }½`ya“^ÉÔｩｰ¦{}Sxp«flı°†³‰zbcp3›'hﾋlp_flı°†³‰}§vx^flı °†gSxnuｴí’^NｳÈ¼ƒNｳ½Ògio^5gòy’ep3}8m‹xb«_µ=y^flı°†’ゴ bdŽ2^œ±”gザÜu2ジê§\^ｵ"ｩｰs})4p«zbce.›|n^ﾑ$y’<u¦†¶C;z nx¾ms}Utm‹xb« ザ)#*ジ _nfn^flı°†)4ﾗ}’½?¦cgüj^flı°†Öカﾗ}ƒ4ﾟﾗ} ›､™}Ú,p«lz’ヴnb_¡u^flı°†’R}½`ya«2ﾊf¤è“6bgヴnj^ﾈｿÀ}¯±Æ„ﾊg »¬àp«¦{ﾇギ⁄a«_ æ†„°àŸÙGŒ’^ãÉ-ｧÝ!7ｻ}§vx^‚-¦{÷F¹}#:r]R Xﾍ›Ý!nuàŸå0 ｾｱya«_( HŒ}¥êƒÙケ^_¦{ÙG}/ﾀ¦M`¦Nｳ›カ‾àp«lzgyh«_¡u^+E æ†„°Œ®³¸yﾚｦÐo_Tザ4;8A0ジ¦{½óµå0･zQﾏnx^…±Œ¬}žp«ñvQザS&V Qヅ Qｽガ‾ジgýhj¦«_su£^4;8A0 §c¦½óµÐo_･¦{}ebxÞgs¦å0géｳz¦« ザ+ジ _sl y^By｠y’flı°†LﾕÉ›ﾌùznuÐo_æ†„°Œヰq›tsznxy｠›ｺvuザð(ジ_s u£}^æ†„°Xﾍ}§«flı°†LﾕÉÇﾟÚ,T›{｡nu_m¤}^flı°†LﾕÉ›ﾌùznuå0･ ähKﾃu£}^æ†„°Xﾍ}L｢nuflı°†LﾕÉ}ﾚｦ›ñànx^å0Þg¦{ﾄÌ›ｺvu_ ２．実験方法 µｶs¦Ł·¸©”-Ł‒}§ vxæ†„°Xﾍﾝôz¦«÷F ›Êｿnu_O}^Êｿnuﾝô÷ F}º©æ³›Ui^dzà*}ﾝ ôf¤ケônu_æ†„°XﾍÆ¯ ³±gﾎÕm‹xb«º©æ³÷F z‚-÷F›½éﾓs}9xnx^ æ†„°Œ›Êｿnu_ flı°†ｯｭÊｿ}’^py}flı°†ｯｭ›)4p«lzgÙfvxb«ø¬¯±Æ„ﾊ›jbu_flı°† 図 1.æ†„°Xﾍ}§«flı°†LﾕÉÇﾟÚ,zNｳà ３次元アミロイド構造体を利用した免疫測定チップの開発 * 真栄城　正　寿 * Development of Immunoassay Chip Using 3D Amyloid-Based Structures *Masatoshi MAEKI *

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Page 1: 3次元アミロイド構造体を利用した免疫測定チップ … › wp-content › themes › test_web › ...174 2017年3月12日受理 * 豊田理研スカラー北海道

174

2017年3月12日受理* 豊田理研スカラー北海道大学大学院工学研究院応用化学部門

3次元アミロイド構造体を利用した免疫測定チップの開発

3 次元アミロイド構造体を利用した

免疫測定チップの開発

Development of Immunoassay Chip Using 3D Amyloid-Based Structures

Masatoshi Maeki

Amyloid fibrils have attracted attention as one of the new nanobiomaterials. The amyloid fibrils show

great features for biomaterial-based applications such as self-assembly, high thermal stability, and high Young’s modulus. However, the growth control of the amyloid fibrils is not well understood in detail. In this study, we demonstrated the methodology for growth control of the amyloid fibrils using a microfluidic device. The 3D amyloid-based structures were applied to a support for immobilization of enzyme. The enzyme immobilized 3D amyloid-based structures enabled the effective reaction compared with the free enzymes.

１．はじめに

S V

２．実験方法

図 1.

３次元アミロイド構造体を利用した免疫測定チップの開発

*真栄城　正　寿*

Development of Immunoassay Chip Using 3D Amyloid-Based Structures

*Masatoshi MaEKi*