MAGITTIEAV KRKIQVLQQQ ADDAEERAER LQREVEGERR AREQAEAEVA SLNRRIQLVE EELDRAQERL ATALQKLEEA

EKAADESERG MKVIENRALK DEEKMELQEI QLKEAKHIAE EADRKYEEVA RKLVIIEGDL ERTEERAELA ESRCREMDEQ

IRLMDQNLKC LSAAEEKYSQ KEDKYEEEIK ILTDKLKEAE TRAEFAERSV AKLEKTIDDL EVYRRKHQEL QAMQMELQSP

EYKLSKLRTS TIMTDYNPNY CFAGKTSSIS DLKEVPRKNI TLIRGLGHGA FGEVYEGQVS GMPNDPSPLQ VAVKTLPEVC

SEQDELDFLM EALIISKFNH QNIVRCIGVS LQSLPRFILL ELMAGGDLKS FLRETRPRPS QPSSLAMLDL LHVARDIACG

CQYLEENHFI HRDIAARNCL LTCPGPGRVA KIGDFGMARD IYRASYYRKG GCAMLPVKWM PPEAFMEGIF TSKTDTWSFG

VLLWEIFSLG YMPYPSKSNQ EVLEFVTSGG RMDPPKNCPG PVYRIMTQCW QHQPEDRPNF AIILERIEYC TQDPDVINTA

LPIEYGPLVE EEEKVPVRPK DPEGVPPLLV SQQAKREEER SPAAPPPLPT TSSGKAAKKP TAAEVSVRVP RGPAVEGGHV

NMAFSQSNPP SELHKVHGSR NKPTSLWNPT YGSWFTEKPT KKNNPIAKKE PHDRGNLGLE GSCTVPPNVA TGRLPGASLL

LEPSSLTANM KEVPLFRLRH FPCGNVNYGY QQQGLPLEAA TAPGAGHYED TILKSKNSMN QPGP

1

81

161

241

321

401

481

721

561

641

fusion site

trypsin digest yields 34.4% coverage including fusion peptide LEKTIDDLE-VYR

IQLVE EELDRIQVLQQQ ADDAEER

IRLMDQNLKC LSAAEEK

CREMDEQ

EYKLSKLR

ILTDKLKEAE TRAEFAER

LVIIEGDL ER

AREQAEAEVA SLNR

PTSLWNPT YGSWFTEKPT K

PRPS QPSSLAMLDL LHVAR

IGDFGMARD IYRASYYRKG GCAMLPVK

HQEL QAMQMELQSP

TSSIS DLKEVPRK

KNCPG PVYR

DPEGVPPLLV SQQAK

LEPSSLTANM KEVPLFRLR

LEKTIDDL EVYR

ALK DEEKMELQEI QLKEAK

LPGASLL

lysine-C digest yields 33.9% coverage including fusion peptide LEKTIDDLE-VYRRK

MAGITTIEAV KRK

DEEKMELQEI QLK

HIAE EADRKYEEVA RK

TRAEFAERSV AK

C LSAAEEKYSQ K

G GCAMLPVKWM PPEAFMEGIF TSK

SNQ EVLEFVTSGG RMDPPK

REEER SPAAPPPLPT TSSGK

VHGSR NKPTSLWNPT YGSWFTEKPT

SKNSMN QPGP

YEEEIK ILTDKLEKTIDDL EVYRRK

LSKLRTS TIMTDYNPNY CFAGK

VPVRPK DPEGVPPLLV SQQAK

KNNPIAK

EKAADESERG MKVIENR

LEEA

glutamic-C digest yields 40.8% coverage including fusion peptide RSVAKLEKTIDDLE-VYRRKHQE

DRPNF AIILERIEYC TQDPDVINTA

AV KRKIQVLQQQ ADDAEERAE GERR ARE

QDELDFLM E

RSV AKLEKTIDDL EVYRRKHQE

VPRKNI TLIRGLGHGA FGE

GIF TSKTDTWSFG

LMAGGDLKS FLRE

LPIEYGPLVE EEE

VLLWEIFSLG YMPYPSKSNQ E

VSVRVP RGPAVE

TAPGAGHYE

VA SLNRRIQLVE EE

SERG MKVIENRALK DEE AKHIAE EADRKYEE MDEQRTEERAE

DKYEEEIK ILTDKLKE

GVPPLLV SQQAKREEE

IRLMDQNLKC LSAAEE

proteinase K digest yields 24.5% coverage including fusion peptide VAKLEKTIDDLE-VYRRKHQEL

V AKLEKTIDDL EVYRRKHQEL

LPIEYGPLVE EEELVPV

VLLWEIFSLG YMPYP

YLEENHFI HRDIAARNCL

TIEAV KRKIQVLQQELDRAQERL ATALQKLEEA

PVYRIMTQCW QHQPED

APPPLPT TSSG

SNPP SELHKVHGSR NKPTSL

PGAGHTED TILKSKNS

DRGNLGLE GSC

EKAADESE

FG

VINTA

VPLFRLRH FPCGNVNYGY QQQGLPLE