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8/3/2019 Nghien Cuu Mot So Tac Dung Duoc Ly Cua Dich Chiet Cay Hoan Ngoc
1/29
Nghin cumts tc dngdc l cadchchitcy hon ngc
ti sinh vin NCKHChuyn ngnh: Ha sinh
Thchin: Sinh vin Phng ThHngLngHngdn: PGS.TS. LngThHng Vn
8/3/2019 Nghien Cuu Mot So Tac Dung Duoc Ly Cua Dich Chiet Cay Hoan Ngoc
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Ti liu chia s ti: wWw.SinhHoc.edu.vn t vn
Gc t do (GTD) l mt trong nhng nguyn nhngy ra s lo ha v rt nhiu cc loi bnh nh:ung th, vim khp, tim mch, i tho ng,nhimc
Cc GTD c thcloibnhcc cht ho hccbitcgi chung l cc chtchng oxy ho(COXH), chng c nhiu trong cc loi rau, c,qu v mt s cy thuc.
Cy HN vn c dn gian s dng nh mt cythuc c kh nng cha rt nhiu bnh khcnhau. Nhng mt s tc dng thc ca chng nhchng oxy ha, c ch mt s vi khun cncha c chng minh mt cch y .
8/3/2019 Nghien Cuu Mot So Tac Dung Duoc Ly Cua Dich Chiet Cay Hoan Ngoc
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Ti liu chia s ti: wWw.SinhHoc.edu.vnMc tiu nghin cu
1. Xc nh hm lng mt s cht c tc dngchng oxy ha trong cy Hon ngc
2. Xc nh kh nngkhng mt s vi khunc hi ca dch chit t l v r cy Honngc .
8/3/2019 Nghien Cuu Mot So Tac Dung Duoc Ly Cua Dich Chiet Cay Hoan Ngoc
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Ti liu chia s ti: wWw.SinhHoc.edu.vn Tng quan ti liu
1. Giithiuchungvcy HonNgc2. Gc tdo v nguyn nhn hnh thnh
gctdo3. Mtshnggiiquytgctdo4. icngvccchtchngoxy ho5. cimcamtschtchngoxy hothngcdng trongdcphm.
8/3/2019 Nghien Cuu Mot So Tac Dung Duoc Ly Cua Dich Chiet Cay Hoan Ngoc
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Ti liu chia s ti: wWw.SinhHoc.edu.vn I TNG NGHIN CU
itngnghin cuMt s b phn ca cyHN c nghin cu gmc:
+ L cy HN
+ R cy HN
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Ti liu chia s ti: wWw.SinhHoc.edu.vnPHNG PHP, VT LIU NGHIN CU
1. Phng php nghin cu:S dngphng php thcnghim, phn tch, m t, so
snh cc mu c lp v sovi i chng.
2. Vt liu nghin cu
S dng 2 chng vi khun lEscherichia. coli ( E.coli )v Bacillus. subtilis (B.Subtilis) th hot tnhkhng sinh ca l v r cy
hon ngc.
8/3/2019 Nghien Cuu Mot So Tac Dung Duoc Ly Cua Dich Chiet Cay Hoan Ngoc
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Ti liu chia s ti: wWw.SinhHoc.edu.vn3. Ho cht:
Gm cc ho cht chnh nh:NaCl, Pepton, petrol ete, bubfer, ethanol, kali hidroxid, Natri
ascobat, methanol, - tocopherol, ascobic acid chun gc...
4. Thitbnghin cu:
+ My cc ph+ My sc k lnghiu cao nng (HPLC)+ Tm nui vi khun
+ Bung nui cy vi khun+ Cn phn tch+ Ni kh trng t+ V mt s thit b my mc khc
8/3/2019 Nghien Cuu Mot So Tac Dung Duoc Ly Cua Dich Chiet Cay Hoan Ngoc
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Ti liu chia s ti: wWw.SinhHoc.edu.vn
H thng my ccph H thng my sc klng hiu cao nng
8/3/2019 Nghien Cuu Mot So Tac Dung Duoc Ly Cua Dich Chiet Cay Hoan Ngoc
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Ti liu chia s ti: wWw.SinhHoc.edu.vnK thut tin hnh
K thut thu dch chit t l, r cy HN.K thut xc nh hot tnh khng khun
ca dch chit t cy HN.K thut xc nh hm lng vitamin E
bng my sc k lng hiu cao nng.K thut xc nh hm lng vitamin C
bng my cc ph.
8/3/2019 Nghien Cuu Mot So Tac Dung Duoc Ly Cua Dich Chiet Cay Hoan Ngoc
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Ti liu chia s ti: wWw.SinhHoc.edu.vnKt qu nghin cu1. Hm lng mt s cht COXH trong cy HN
Hm lng vitamin C trong cc mu dch chitm /k kh
Biu hm lng vitamin C trong cc mu dch chit
42.0854
0.88884.6478
0.071705
10152025
30354045
L ti L kh R ti R kh Tn mu
Hml
ngVT
MC
(mg/kgkh)
Hm lng
8/3/2019 Nghien Cuu Mot So Tac Dung Duoc Ly Cua Dich Chiet Cay Hoan Ngoc
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Ti liu chia s ti: wWw.SinhHoc.edu.vnBi u hm l- ng VTM E trong c c mu dch chit
53.0729
0 0
13.3977
0
10
20
30
40
5060
L t- i Rt- i Rkh L khM u
Hml-ngVTME(mg/
kg
kh
Hm l- ng
Hm lng vitamin E trong cc mu dch chit
(mg/kg kh)
8/3/2019 Nghien Cuu Mot So Tac Dung Duoc Ly Cua Dich Chiet Cay Hoan Ngoc
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Ti liu chia s ti: wWw.SinhHoc.edu.vn2. Kt qu th tc dng khng khun
nh chp mt mu thnghim th hot tnh khngkhun sau 30 gi nui cy.
Mu i chng (gia)Cc mu th dch chit
cy HN cc nng khc
nhau (xung quanh)
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Ti liu chia s ti: wWw.SinhHoc.edu.vn2.1.So snh kh nng khng khun ca ccmu dch chit cy HN gia hai loi vi khun
Kh nng khng khun ca dch chit l ti honngc (chit bng nc ct) trn hai loi vi khun
0.7330.333
0.867
0.433
1.333
0.533
00.20.40.60.8
11.21.4d(cm)
0,125 0,25 0,5Nng (g/ml)
Vi khun B. subtilis.Vi khun E. coli
Biu so snh kh nng khng khun
cadch chit l ti cy HN chit bng ncct
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Ti liu chia s ti: wWw.SinhHoc.edu.vn
Kh nng khng khun ca dch chit l ti HN(chit bng NaCl 0.9%) trn hai loi vi khun
Biu so snh kh nng khng khun ca dch chitl ti cy HN chit bng NaCl 0.9 %
d (cm)
0.4330.533 0.5
0.6
0.90.833
00.10.20.30.4
0.50.60.70.80.9
0,125 0,25 0,5Nng (g/ml)
Vi khun B. subtilisVi khun E. coli
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Ti liu chia s ti: wWw.SinhHoc.edu.vn
Kh nng khng khun cadch chit rti(chit bngnc ct) trn hai loi vi khun
Biu so snh kh nng khng khun ca dch chitr ti cy HN chit bng nc ct
0.5330.567
0.7330.7
0.967
0.767
00.10.20.30.40.50.60.70.80.9
1d(cm)
0,125 0,25 0,5
Nng (g/ml)
Vi khun B. subtilis.Vi khun E. coli. i
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Ti liu chia s ti: wWw.SinhHoc.edu.vn
Kh nng khng khun ca dch chit rti
(chit bng NaCl 0.9%) trn hai loi vi khun
Biu so snh kh nng khng khun ca dch chitr ti cy HN chit bng NaCl 0.9%
0.7
0.533
1
0.733
1.033
0.677
0
0.2
0.4
0.6
0.8
1
1.2d(cm)
0,125 0,25 0,5
Nng (g/ml)
Vi khun B. subtilis.
Vi khun E. coli.
l h h d
2 2 S h kh kh kh
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Ti liu chia s ti: wWw.SinhHoc.edu.vn
Hot tnh khng vi khun B. subtilis ca dch
chit l v r ti cy HN chit bng nc ct
2.2. So snh kh nng khng khun ca cc mudch chit cy HN th trn B. subtilis
00.2
0.4
0.6
0.8
1d (cm)
Biu so s nh ho t tnh kh ng vi khun B.subtilis
ca dch chit l v rt- i cy HN chit bng n- c ctHLTHRT
0.125 0.25 0.5Nng (g/ml)
0.7330.533
0.867
0.733
1.333 0.967
Ti li hi i W Si hH d
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Ti liu chia s ti: wWw.SinhHoc.edu.vn
Hot tnh khng vi khun B. subtilis ca dchchit l v r ti cy HN chit bng NaCl
0.9%
0
0.2
0.4
0.6
0.8
1d (cm)
Biu so s nh ho t tnh kh ng vi khun B.subtilisca dch chit l v rt- i cy HN chit bng NaCl
0.9% NLT
NRT
0.125 0.25 0.5Nng (g/ml)
0.433
0.7
0.5
10.9
1.033
Ti li hi t i W Si hH d
2 3 S h kh kh kh
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Ti liu chia s ti: wWw.SinhHoc.edu.vn
Hot tnh khng vi khun E. coli ca dch chit l
v r ti cy HN chit bng nc ct
2.3. So snh kh nng khng khun ca cc mudch chit cy HN th trn E. coli
0
0.2
0.4
0.6
0.8
1d (cm)
Biu so s nh ho t tnh kh ng vi khun E.coli ca
dch chit l v rt- i cy HN chit bng n- c ct
HLTHRT
0.125 0.25 0.5 Nn /ml
0.333
0.5670.433
0.7 0.533
0.767
Ti li hi t i W Si hH d
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Ti liu chia s ti: wWw.SinhHoc.edu.vn
Hot tnh khng vi khun E. coli ca dchchit l v r ti cy HN chit bng NaCl
0.9%
0
0.2
0.4
0.6
0.8
1d (cm)
Biu so s nh ho t tnh kh ng vi khun E.coli cadch chit l v rt- i cy HN chit bng NaCl 0.9%
NLTNRT
0.125 0.25 0.5 Nng (g/ml)
0.533 0.533 0.60.677
0.8330.733
Ti li hi t i W Si hH d
3 4 S h h t t h kh kh d h
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Ti liu chia s ti: wWw.SinhHoc.edu.vn
Hot tnh khng vi khun B.subtilis ca dch
chit l ti cy HN gia hai dung mi khc nhau
3.4. So snh hot tnh khng khun ca cc mu dchchit khi chit cc dung mi khc nhau
00.2
0.4
0.60.8
1
1.2
1.4
d (cm)
Biu so s nh ho t tnh kh ng vi khun B.subtilis ca dchchit l t- i cy HN gia hai dung mi kh c nhau
n- c ct
NaCl 0.9%
0.733
0.433
0.867
0.5
1.333
0.9
Nng (g/ml)0.125 0.25 0.5
Ti li hi t i W Si hH d
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Ti liu chia s ti: wWw.SinhHoc.edu.vn
Hot tnh khng vi khun B.subtilis ca dchchit r ti cy HN gia hai dung mi khc
nhau
0
0.2
0.4
0.6
0.8
1
1.2d (cm)
Biu so s nh hot tnh kh ng vi khun B.subtilis cadch chit rt- i cy HN gia hai dung mi kh c nhau
n- c ct
NaCl 0.9%
0.533
0.7 0.733
1 0.967
1.033
Nng (g/ml)0.125 0.25 0.5
Ti li hi t i W Si hH d
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Ti liu chia s ti: wWw.SinhHoc.edu.vn
Hot tnh khng vi khun E. coli ca dchchit l ti cy HN gia hai dung mi khc
nhau
0
0.2
0.40.6
0.8
1
1.2d (cm)
Biu so s nh ho t tnh kh ng vi khun E.coli ca dchchit l t- i cy HN gia hai dung mi kh c nhau
n- c ct
NaCl 0.9%
0.3330.533 0.433
0.6
0.533
0.833
Nng (g/ml)0.125 0.25 0.5
Ti liu chia s ti: wWw SinhHoc edu vn
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Ti liu chia s ti: wWw.SinhHoc.edu.vn
Hot tnh khng vi khun E. coli ca dchchit r ti cy HN gia hai dung mi khc
nhau
0
0.2
0.40.6
0.8
1
1.2d (cm)
Biu so s nh ho t tnh kh ng vi khun E.coli ca dchchit rt- i cy HN gia hai dung mi kh c nhau
n- c ct
NaCl 0.9%
0.5760.533
0.7 0.6770.767 0.733
Nn /ml0.125 0.25 0.5
Ti liu chia s ti: wWw SinhHoc edu vn
8/3/2019 Nghien Cuu Mot So Tac Dung Duoc Ly Cua Dich Chiet Cay Hoan Ngoc
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Ti liu chia s ti: wWw.SinhHoc.edu.vnNhn xt chungCc kt qunghin cu ca chng ti cho thy:
+ Cc b phn ca cy HN c tc dng d-c l r rng bi n c kh nng c ch cc vikhun gy bnh v n c cha mt s chtCOXH.
+ Hm lng cc cht COXH trong l kh
cao nhng trong r th rt t.+ Cng t nghin cu ca chng ti chothy cy HN ch c tc dng dc l tt khi
cn ti. Khi sy kh th t c dng ca n
Ti liu chia s ti: wWw SinhHoc edu vn
Kt l
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Ti liu chia s ti: wWw.SinhHoc.edu.vnKt lun1. V hm lng cc cht COXH trong cy HN:
- Tt c cc mu l v r u cha vitamin C.
Trong , mu l c hm lng vitamin C cao hn rtnhiu so vi mu r; mu ti c hm lng cao hnnhiu so vi mu kh (P < 0.001).- Vitamin E ch c trong mu l (c ti v kh) vihm lng kh cao trong hm lng vitamin E l ti
cao hn rt nhiu so vi l kh, cn trong r (c tiv kh) khng tm thy vitamin E (P < 0.001).
2. V kh nng khng khun ca dch chitcy HN- Tt c cc mu ti (l v r) ca cy HN chitbng nc ct v NaCl 0,9% u c kh nng khnghai chng vi khun th nghim (B. subtilis vE.coli), cn i vi mu kh (l v r) u khng ckhnng khng hai chng vi khun th nghim ni
trn.
Ti liu chia s ti: wWw SinhHoc edu vn
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Ti liu chia s ti: wWw.SinhHoc.edu.vn- Hot tnh khng B. subtilis ca cc mu dch chitcy HN ti c s khc nhau:+ Mu l ti chit bng nc ct c hot tnh cao hn so
vi mu l ti chit bng NaCl 0.9% v cao hn mu rti chit bng nc ct (p0.05).- Cc mu l ti chit bng nc ct v r ti chit bngNaCl 0.9% c hot tnh khng B. subtiliscao hn so viE. coli(P < 0.05).- Cc mu l ti chit bng NaCl 0.9% v r ti chitbng nc ct c hot tnh khng hai loi vi khun trn lnh nhau (P > 0.05).
Ti liu chia s ti: wWw SinhHoc edu vn
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Ti liu chia s ti: wWw.SinhHoc.edu.vnKin ngh
Nn tip tc th hot tnh khng khun cacy HN vi cc chng vi khun khc.
Tip tc xc nh hm lng mt s thnh
phn ho sinh khc c kh nng COXH vkhng sinh trong cy HN nh selenium,carotenoit, flavonoit, alkaloit...
Th nghim tc dng COXH v khng khunca cy HN trn ng vt thc nghim
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