C a p t u r e m ™ A n t i b o d y P u r i f i c a t i o n

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• High-capacity Protein A membranes

• 신속한 항체 스크리닝 (antibody screening)에 최적

• 효율적인 IP & Co-IP (Immunoprecipitation) 실험 가능

• 배지 교체 (media exchange) 불필요

☐ Save Time

☐ High Quality

☐ High Purity

☐ No Contamination

실온에서 5분이면 항체 정제 완료

항체의 응집/실활을 방지하여 고품질의 항체 정제

오염 물질이 거의 없는 고순도 항체 정제

정제 과정 중의 오염 또는 carryover 방지

대~박! 항체를 DNA처럼 Miniprep Kit로 정제?

Capturem™ Protein A Miniprep Columns

Capturem™ Protein A Miniprep (Code 635717)

Capturem™ Protein ARapid Antibody Purification

The Next Generation of Antibody Purification

실온에서 5분이면 항체 정제 완료!

그림 1. Capturem™ membrane technology for Protein A purification

Modified membrane pores

그림 2. Animal sera antibody binding test

다양한 동물 유래의 혈청 (animal sera)으로부터 Capturem™

Protein A Miniprep Spin Column을 이용하여 고순도의 항체를

정제할 수 있었다.

Marke

rSh

eep

Goat

Rat

Mou

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man

Rabb

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rse

* Sample: animal sera, ascites fluid, cell culture media 등

* Compatible with all standard buffers

* 정제수율: 300 ㎍ / column (binding capacity: ≥ 75 mg/㎖)

* 정제시간: 5분 (~15분)

샘플가능

Animal sera

Sheep

Goat

Rat

Mouse

Human

Rabbit

Horse

Purified antibody (㎍)

256

184

170

140

129

102

215

C a p t u r e m ™ P r o t e i n & A n t i b o d y P u r i f i c a t i o n

www.takara.co.kr

고 객 (제품문의) 02-2081-2510

지원센터 support@takara.co.kr

042-828-6525 대 전 지 사

(대전•충청지역 제품주문)

☐ Membrane Column

☐ No Cold Room

☐ Rapid Prep

☐ Simple Process

새로운 형태의 단백질 정제 기술

실온에서 정제 OK

5 ~ 15분이면 고순도의 단백질 정제 가능

Equilibration, Binding, Wash, Elute 끝!

Capturem™ 은 독창적인 nylon-based membrane을 이용한 Clontech만의 획기적인 단백질 정제 기술입니다.

단백질과 항체를 DNA처럼 간단하게 Prep Kit로 정제하세요!

자세한 정보는 다카라코리아로 문의하세요

TALON® Resin - Cobalt (Co2+) IMAC Resin

- 고(高)순도의 특이성 높은 His-Tagged 단백질 정제

- Gravity (Batch) / Superflow (FPLC) / Magnetic

Thiophilic-Superflow Resin (Code 635616 외)- Thiophilic affinity chromatography (TAC) 방식의 항체 정제용 레진

- Protein A로 정제가 어려운 항체도 광범위하게 정제 가능

- IgG, IgM, IgA, Fab & Fc fragments, C3 & C4 complement factors

His60 Ni Resin

- Nickel (Ni2+) IMAC Resin

- 보다 높은 고(高)수율의 His-Tagged 단백질 정제

- Gravity / Superflow (FPLC) / Magnetic

Capturem™ Technology

Resins for Protein Purification

Capturem™ 제품리스트

제품명Code 용량 Note

Capturem™ Protein A Miniprep

Capturem™ Protein A 96

Capturem™ His-Tagged Purification Miniprep Kit

Capturem™ His-Tagged Purification Maxiprep Kit

Capturem™ His-Tagged Purification 96

Capturem™ His-Tagged Purification Maxiprep Columns

Antibody Purification - column 형태의 항체 정제

His-Tagged Protein Purification – column 형태의 His-Tagged 단백질 정제

635717

635716

12 Purifications

96 Purifications

12개의 disposable spin columns 포함

96well plate의 high-throughput system

635710

635713

635714

635719

20 Purifications

6 Purifications

96 Purifications

50 Columns

100 ㎍ (농도 0.3 ~ 1 mg/㎖) 단백질 정제

2.5 mg (농도 4.5 mg/㎖) 단백질 정제

96well plate의 high-throughput system

Maxiprep column만 별도 구매

• Capturem™으로 양호한 단백질 발현 및 정제를 확인했다면?

• 보다 많은 양의 large scale 단백질 정제를 원한다면?

at room temperture

minutes!Only 5

Clontechonly

A Takara Bio Company

www.clontech.com

Clontech Laboratories, Inc.

P r o t e i n e x P r e s s i o n & P u r i f i c a t i o n

IgY IgM

IgG IgE

Broader selectivity

Thiophilic Antibody Purification Resins

An efficient, versatile, and economical alternative to Protein A purification

Clontech’s Thiophilic-Uniflow and -Superflow Resins provide highly stable

purified antibodies with the following advantages over conventional Protein A

antibody purification methods:

• Purification at neutral pH—avoid antibody aggregates

• High capacity (20–25 mg Ab/ml resin)—get high recoveries

• Broader selectivity—purify IgY, IgM, IgE, and scAb

• Fewer purification steps—save time

• Reusable resin—save money

• Higher stability of the purified product

Protein A vs. Thiophilic Resin

Historically, Protein A has been the preferred method of immunoglobulin purification. However, there are certain types of antibodies, such as the single-chain antibodies IgE, IgY, and IgM, that cannot be purified using Protein A. Thiophilic adsorption chromatography is ideal for these types of applications, as well as immunoglobulin purification in general.

In addition to providing broader selectivity and higher stability, thiophilic adsorp-tion chromatography offers a faster protocol than Protein A under neutral buffer conditions (Figure 1). Purification with Thiophilic Resin allows elution in any buffer with pH 7.0. In contrast, Protein A requires elution in a buffer with pH lower than 7.0. Protein A also requires a dialysis step, which can be omitted with Thiophilic Resin.

Figure 1. Thiophilic Resin purifies antibodies at neutral pH and in less time than Protein A.

11.11 IN (633352)

United States/Canada: +1.800.662.2566 • Asia Pacific: +1.650.919.7300 • Europe: +33.(0)1.3904.6880 • Japan: +81.(0)77.543.724For Research Use Only. Not for use in diagnostic or therapeutic procedures. Not for resale. Clontech and the Clontech logo are trademarks of Clontech Laboratories, Inc. All other marks are the property of their respective owners. Certain trademarks may not be registered in all jurisdictions. ©2011 Clontech Laboratories, Inc.

Elute in low pHbuffer (pH ≤ 3.0)

2 daysProtein A

Filter/Centrifuge

Load Wash Performdialysis

Concentrateprotein

Elute in neutralbuffer (pH ≤ 7.0)

Thiophillic Resin

Filter/Centrifuge

Addsalt

Load Wash