ЭНЗИМАТИЧЕСКИЕ МЕТОДЫ КОНВЕРСИИ БИОМАССЫ: СОВРЕМЕННОЕ СОСТОЯНИЕ

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EG-G. . () CBH

(C5 C6)

10 FPU / 1 + 20 bGU / , [S] = 100 /, pH 5, 50C, 24 h / / / / / / / ()

, -, () - : Trichoderma sp. Penicillium sp. Aspergillus sp.

: (CBH1, CBH2, Eg1, Eg2, Eg3, .)- (, -1,4/1,3-) (-, -, -, , -, -, .) (-, ) (-, , .) (-, -)

:Penicillium 537Penicillium PCA Aspergillus sp.

(I) - , - ,

,

. , 10 FPU / 1 , [S] = 5%, 5, 50 PenicilliumTrichoderma

-

Penicillium Trichoderma

, Trichoderma

Penicillium Trichoderma Penicillium , Trichoderma

: - ()

( , ) (, ) ( ) () , ( , ) (1-, 3- ), , ,

(II) () -

-

() (II)

- Penicillium 537 (CBH1 ) -1,4- Penicillium 537(CBH1 )

-GEg

() - (II) -1,4- Penicillium 537 (CBH1 ) -1,4- Penicillium 537(CBH1 ) XylMan

Penicillium 537 (II). 537-Cell. 537-Xyl. 537-bGl .537-Cell .537-Xyl .537-bGL .537-Cell .537-Xyl .537-bGl Cell + Xyl + bGl

Penicillium 537 (II)

. 537-Cell

. 537-Xyl

. 537-bGl 537-Cell + 537-Xyl + 537-bGl

Cell + Xyl + bGl

(III) ()

() (III)

- Penicillium 537(Hist )

5 / []- 5%, 5.0, 50C

1 , 24 , -G

4

6.14987925.3047619048

7.246087.1037037037

7.8809287.55

2

: 50, 50 / S, 2 , 1000rpm, V=1,5

-

1mgE/1gSRA610Crs, g/l24mgE/1gSRA610Crs, g/l

3-255.1.2P.verr 221-1512.0100.84930.98B1-221-1512.0800.61566.15

pHist1--Glu12.0200.64851.97pHist1 cl.22.0800.56877.25


-

1mgE/1gSRA490Cglc, g/l24mgE/1gSRA490Cglc, g/l

3-255.1.2P.verr 221-1512.050.47690.95B1-221-1512.0200.66845.30



: 50, 50 / S, 2 , 1000rpm, V=1,5 . - F10 3-194.1.2 (30% 1 S)

-

1mgE/1gSRA610Crs, g/l24 mgE/1gSRA610Crs, g/l

3-255.1.2P.verr 221-1512.0200.78251.84B1-221-1512.01600.35648.57



F100.6100.47970.65F100.6800.22923.37

-

1mgE/1gSRA490Cglc, g/l24 mgE/1gSRA490Cglc, g/l

3-255.1.2P.verr 221-1512.050.85961.71B1-221-1512.0400.43026.83



F10F100.6100.15640.63F100.6400.21183.39

2

[], /

[], /

5

1, 50 / S, 50, 2 / 1 S, 0,7 F10 1 S, 1

10

1, 50 / S, 50, 2 / 1 S, 0,7 F10 1 S, 24

: 50, 50 / S, 5 , 1000rpm, V=1,5

-


3-255.1.2B1-221-1515.0400.42462.395.01600.500910.65

pHist1 cl.215.0400.52613.4515.01600.494213.28

pHist4 cl.325.0400.5423.5125.01600.510213.54

pHist5 cl.335.0200.88132.4535.01600.390311.58

pHist6 cl.145.0400.37582.8445.01600.425212.15

-


3-255.1.2B1-221-1515.0100.44631.775.0400.57779.17

pHist1 cl.215.0100.65443.1215.0400.659312.21

pHist4 cl.325.0100.70813.3725.0400.696112.89

pHist5 cl.335.0100.38191.8235.0400.543510.06

pHist6 cl.145.0100.42912.0445.0400.57810.70

: 50, 50 / S, 5 , 1000rpm, V=1,5 . - F10 3-194.1.2 (30% 1 S)

-


3-255.1.2B1-221-1515.0400.64953.205.02000.550714.21

pHist1 cl.215.0400.58923.7115.02000.468516.07

pHist4 cl.325.0400.64953.9525.02000.461315.92

pHist5 cl.335.0400.66154.0035.02000.5116.92

pHist6 cl.145.0500.55264.4545.02000.588418.52

F101.5100.47970.65F101.5800.22923.37

-


3-255.1.2B1-221-1515.0100.73292.915.0800.375511.92

pHist1 cl.215.0200.37723.5915.0800.420515.57

pHist4 cl.325.0200.35883.4225.0800.425115.74

pHist5 cl.335.0200.37623.5835.0800.450116.67

pHist6 cl.145.0200.41713.9745.0800.489618.13

F10F101.5100.15640.63F101.5400.21183.39

00

00

00

00

00

1, 50/ S, 50, 5 / 1 S, 1

F10

00

00

00

00

00

00

1, 50 / S, 50, 5 / 1 S, 1,7 F10 1 S, 1

00

00

00

00

00

1, 50/ S, 50, 5 / 1 S, 24

00

00

00

00

00

00

1, 50 / S, 50, 5 / 1 S, 1,7 F10 1 S, 24

: 50, 50 / S, 10 , 1000rpm, V=1,5

-


3-255.1.2B1-221-151110.0400.68113.3110.02000.658616.15

pHist1 cl.2210.0500.58454.61110.02000.660920.00

pHist4 cl.3310.0500.60514.71210.02000.576318.27

pHist5 cl.310.0400.56643.61310.02000.479716.30

pHist6 cl.1410.0400.68514.10410.02000.566918.08

-


3-255.1.2B1-221-151110.0200.35762.8410.0800.451814.34

pHist1 cl.2210.0200.45754.36110.0800.486818.03

pHist4 cl.3310.0200.48054.58210.0800.476217.64

pHist5 cl.310.0200.29852.84310.0800.422815.66

pHist6 cl.1410.0200.32473.09410.0800.471217.45

: 50, 50 / S, 10 , 1000rpm, V=1,5 . - F10 3-194.1.2 (30% 1 S)

-


3-255.1.2B1-221-15110.0800.47025.1010.02000.869819.95

pHist1 cl.2110.0800.3345.33110.02000.786122.55

pHist4 cl.3210.0800.34365.41210.02000.7280121.37

pHist5 cl.3310.0800.44666.25310.02000.908925.06

pHist6 cl.1410.0800.36655.60410.02000.897724.83

F103.0100.47970.65F103.0800.22923.37

-


3-255.1.2B1-221-1518.3200.56334.4710.0800.587818.66

pHist1 cl.217.5200.54545.19110.0800.59622.07

pHist4 cl.325.0200.50324.79210.0800.550220.38

pHist5 cl.33200.55015.24310.0800.654224.23

pHist6 cl.147.9200.54185.16410.0800.641723.77

F10F103100.15640.63F103400.21183.39

00

00

00

00

00

1, 50/ S, 50, 10 / 1 S, 1

00

00

00

00

00

00

1, 50 / S, 50, 10 / 1 S, 3,4 F10 1 S, 1

00

00

00

00

00

1, 50/ S, 50, 10 / 1 S, 24

00

00

00

00

00

00

1, 50 / S, 50, 10 / 1 S, 3,4 F10 1 S, 24

C, mg/g C, mg/g 280pNPh-b-Glu, U/mgCMC, U/mgAvicel, U/mgCellobiose, U/mg

pHist1 cl.27037422.4221.090.624.06

pHist4 cl.35296874.4315.470.797.58

pHist5 cl.36256930.9712.990.590.73

pHist6 cl.15896531.214.810.740.79

, # 87.6

1: 2 1

-3 2448,

RA610[RS], g/lRA610[RS], g/lRA610[RS], g/l3

3-255.1.2B1-221-151400.34952.121000.5797.361200.56828.7124

pHist1 cl.2400.59113.711200.49719.991600.440412.4048

pHist4 cl.3400.63483.891200.521810.301600.454412.63

pHist5 cl.3400.27612.431200.3598.301600.358411.06

pHist6 cl.1400.39592.921200.42529.111600.414311.97

-10.37170.0510.37170.0510.33050.05

-

RA490[Glc], g/lRA490[Glc], g/lRA490[Glc], g/l

3-255.1.2B1-221-151200.20671.58400.33415.12400.40466.20

pHist1 cl.2200.25262.17400.43957.55400.50518.67

pHist4 cl.3200.3042.61400.44267.60400.49138.43

pHist5 cl.3200.14251.22400.29695.10400.38556.62

pHist6 cl.1200.18081.55400.32345.55400.42327.27

-20.0360.0320.0340.0310.09260.04

1: 5 1

-3 24 48

RA610[RS], g/lRA610[RS], g/lRA610[RS], g/l

3-255.1.2B1-221-151800.35574.281600.733814.002000.585114.83

pHist1 cl.2800.51486.811600.50813.502000.445415.60

pHist4 cl.3800.57197.271600.563514.412000.422415.13

pHist5 cl.3800.31155.151600.43112.242000.393914.55

pHist6 cl.1800.46166.371600.516313.642000.45415.78

-


3-255.1.2B1-221-151400.17412.67800.28058.60800.3049.32

pHist1 cl.2400.31975.49800.314210.79800.330811.36

pHist4 cl.3400.34385.90800.327611.25800.319810.98

pHist5 cl.3400.17453.00800.28729.86800.295510.15

pHist6 cl.1400.19573.36800.311710.70800.325311.17

1: 10 1

-3 24 48


3-255.1.2B1-221-1511600.22896.743200.236713.703200.29715.43

pHist1 cl.21600.313810.332000.548317.702000.578618.32

pHist4 cl.31600.326110.532000.552117.782000.56818.10

pHist5 cl.3800.59447.462000.466116.022000.563118.00

pHist6 cl.1800.71928.472000.551817.772000.586318.47

-


3-255.1.2B1-221-151800.16014.911600.169810.411600.184711.32

pHist1 cl.2400.46167.92800.400713.76800.396713.62

pHist4 cl.3400.47838.21800.388713.35800.389213.36

pHist5 cl.3400.28864.95800.364312.51800.393113.50

pHist6 cl.1400.32245.53800.38713.29800.39313.49

00000

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B1-221-151

pHist1 cl.2

pHist4 cl.3

pHist5 cl.3

pHist6 cl.1

,

[], /

, 2 1

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00000

00000

B1-221-151

pHist1 cl.2

pHist4 cl.3

pHist5 cl.3

pHist6 cl.1

,

[], /

, 2 1

00

00

00

00

00

00

, 2 , 3

00

00

00

00

00

00

, 2 , 24

00000

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B1-221-151

pHist1 cl.2

pHist4 cl.3

pHist5 cl.3

pHist6 cl.1

,

[], /

, 5 1

00000

00000

00000

B1-221-151

pHist1 cl.2

pHist4 cl.3

pHist5 cl.3

pHist6 cl.1

,

[], /

, 5 1

00

00

00

00

00

, 5 , 3

00

00

00

00

00

, 5 , 24

00000

00000

00000

B1-221-151

pHist1 cl.2

pHist4 cl.3

pHist5 cl.3

pHist6 cl.1

,

[], /

, 10 1

00000

00000

00000

B1-221-151

pHist1 cl.2

pHist4 cl.3

pHist5 cl.3

pHist6 cl.1

,

[], /

, 10 1

00

00

00

00

00

, 10 , 3

00

00

00

00

00

, 10 , 24

00

00

00

00

00

00

, 2 , 48

00

00

00

00

00

, 5 , 48

00

00

00

00

00

, 10 , 48

, # 87.6

1: 2 1

-3 2448,


3-255.1.2B1-221-151400.62083.091180.63149.241180.69879.9524

pHist1 cl.2800.25124.661600.413810.541600.473711.4148

pHist4 cl.3800.21224.341600.30729.011600.457711.18

pHist5 cl.3800.20524.281600.379110.051600.425910.72

pHist6 cl.1800.30625.101600.462711.251600.463111.25

-


3-255.1.2B1-221-151200.28912.22400.43746.73400.50017.66

pHist1 cl.2200.34892.99400.47538.16400.52198.96

pHist4 cl.3200.38123.27400.50998.75400.55679.56

pHist5 cl.3200.3693.17400.46237.94400.53779.23

pHist6 cl.1200.41913.60400.52449.00400.596210.24

1: 5 1

-3 24 48


3-255.1.2B1-221-151800.70246.771600.816115.182000.69916.87

pHist1 cl.21000.44687.811600.566914.462000.444715.59

pHist4 cl.31000.41067.441600.579214.662000.43915.47

pHist5 cl.31000.41547.491600.629715.492000.484216.39

pHist6 cl.11000.52878.651600.705516.722000.51717.06

-


3-255.1.2B1-221-151400.30884.75800.31019.54800.351610.78

pHist1 cl.2400.37916.51800.344711.84800.374512.86

pHist4 cl.3400.35396.08800.333511.45800.345211.85

pHist5 cl.3400.35586.11800.367412.61800.36712.60

pHist6 cl.1400.41687.16800.380213.05800.379113.02

1: 10 1

-3 24 48


3-255.1.2B1-221-1511600.58111.803200.321716.143200.348216.91

pHist1 cl.21600.364411.162000.597518.702000.620919.18

pHist4 cl.31600.349310.912000.510516.932000.52617.24

pHist5 cl.31600.371911.282000.591318.582000.641419.60

pHist6 cl.11600.401711.772000.642219.612000.642819.63

-


3-255.1.2B1-221-151800.23027.08800.366711.28800.384611.79

pHist1 cl.2400.50468.66800.401913.80800.420514.44

pHist4 cl.3400.49068.42800.361512.41800.374513.50

pHist5 cl.3400.50668.70800.410114.08800.441115.90

pHist6 cl.1400.57189.82800.428314.71800.428415.44

-3 24 48


F10 3-194.1.20,6 / 1 S100.10420.31100.68080.83200.50441.34

F10 3-194.1.21,7 / 1 S100.41750.59200.76341.80400.57582.93

F10 3-194.1.23 / 1 S200.33021.02400.64173.17800.44284.91

-


F10 3-194.1.20,6 / 1 S40.15050.2350.37930.73100.27141.04

F10 3-194.1.21,7 / 1 S50.2190.4250.80731.55100.55852.14

F10 3-194.1.23 / 1 S100.17540.67100.69942.69200.46093.53

00000

00000

00000

B1-221-151

pHist1 cl.2

pHist4 cl.3

pHist5 cl.3

pHist6 cl.1

,

[], /

, 2 1

00000

00000

00000

B1-221-151

pHist1 cl.2

pHist4 cl.3

pHist5 cl.3

pHist6 cl.1

,

[], /

, 2 1

00

00

00

00

00

, 2 , 3

00

00

00

00

00

, 2 , 24

00000

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B1-221-151

pHist1 cl.2

pHist4 cl.3

pHist5 cl.3

pHist6 cl.1

,

[], /

, 5 1

00000

00000

00000

B1-221-151

pHist1 cl.2

pHist4 cl.3

pHist5 cl.3

pHist6 cl.1

,

[], /

, 5 1

00

00

00

00

00

, 5 , 3

00

00

00

00

00

, 5 , 24

00000

00000

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B1-221-151

pHist1 cl.2

pHist4 cl.3

pHist5 cl.3

pHist6 cl.1

,

[], /

, 10 1

00000

00000

00000

B1-221-151

pHist1 cl.2

pHist4 cl.3

pHist5 cl.3

pHist6 cl.1

,

[], /

, 10 1

00

00

00

00

00

, 10 , 3

00

00

00

00

00

, 10 , 24

00

00

00

00

00

, 2 , 48

00

00

00

00

00

, 5 , 48

00

00

00

00

00

, 10 , 48

000

000

000

0,6 / 1 S

1,7 / 1 S

3 /1 S

,

[], /

000

000

000

0,6 / 1 S

1,7 / 1 S

3 /1 S

,

[], /

00

00

00

[], /

, 3

00

00

00

[], /

, 24

00

00

00

[], /

, 48

7

10.64991369.1698412698

13.2763212.2092592593

13.5374412.8907407407

2

: 50, 50 / S, 2 , 1000rpm, V=1,5

-


3-255.1.2P.verr 221-1512.0100.84930.98B1-221-1512.0800.61566.15



-


3-255.1.2P.verr 221-1512.050.47690.95B1-221-1512.0200.66845.30



: 50, 50 / S, 2 , 1000rpm, V=1,5 . - F10 3-194.1.2 (30% 1 S)

-


3-255.1.2P.verr 221-1512.0200.78251.84B1-221-1512.01600.35648.57



F100.6100.47970.65F100.6800.22923.37

-


3-255.1.2P.verr 221-1512.050.85961.71B1-221-1512.0400.43026.83



F10F100.6100.15640.63F100.6400.21183.39

2

[], /

[], /

5

1, 50 / S, 50, 2 / 1 S, 0,7 F10 1 S, 1

10

1, 50 / S, 50, 2 / 1 S, 0,7 F10 1 S, 24

: 50, 50 / S, 5 , 1000rpm, V=1,5

-


3-255.1.2P.verr 221-1515.0400.42462.395.01600.500910.65

pHist1--Glu15.0400.52613.4515.01600.494213.28

pHist4--Glu25.0400.5423.5125.01600.510213.54

-


3-255.1.2P.verr 221-1515.0100.44631.775.0400.57779.17

pHist1--Glu15.0100.65443.1215.0400.659312.21

pHist4--Glu25.0100.70813.3725.0400.696112.89

: 50, 50 / S, 5 , 1000rpm, V=1,5 . - F10 3-194.1.2 (30% 1 S)

-


3-255.1.2P.verr 221-1515.0400.64953.205.02000.550714.21

pHist1--Glu15.0400.58923.7115.02000.468516.07

pHist4--Glu25.0400.64953.9525.02000.461315.92

F101.5100.47970.65F101.5800.22923.37

-


3-255.1.2P.verr 221-1515.0100.73292.915.0800.375511.92

pHist1--Glu15.0200.37723.5915.0800.420515.57

pHist4--Glu25.0200.35883.4225.0800.425115.74

F10F101.5100.15640.63F101.5400.21183.39

P.verr 221-151P.verr 221-151

pHist1--GlupHist1--Glu


F10

P.verr 221-151P.verr 221-151



F10F10

1, 50 / S, 50, 5 / 1 S, 1,7 F10 1 S, 1

P.verr 221-151P.verr 221-151



P.verr 221-151P.verr 221-151



F10F10

1, 50 / S, 50, 5 / 1 S, 1,7 F10 1 S, 24

: 50, 50 / S, 10 , 1000rpm, V=1,5

-


3-255.1.2B1-221-151110.0400.68113.3110.02000.658616.15

pHist1 cl.2210.0500.58454.61110.02000.660920.00

pHist4 cl.3310.0500.60514.71210.02000.576318.27

pHist5 cl.310.0400.56643.61310.02000.479716.30

pHist6 cl.1410.0400.68514.10410.02000.566918.08

-


3-255.1.2B1-221-151110.0200.35762.8410.0800.451814.34

pHist1 cl.2210.0200.45754.36110.0800.486818.03

pHist4 cl.3310.0200.48054.58210.0800.476217.64

pHist5 cl.310.0200.29852.84310.0800.422815.66

pHist6 cl.1410.0200.32473.09410.0800.471217.45

: 50, 50 / S, 10 , 1000rpm, V=1,5 . - F10 3-194.1.2 (30% 1 S)

-


3-255.1.2B1-221-15110.0800.47025.1010.02000.869819.95

pHist1 cl.2110.0800.3345.33110.02000.786122.55

pHist4 cl.3210.0800.34365.41210.02000.7280121.37

pHist5 cl.3310.0800.44666.25310.02000.908925.06

pHist6 cl.1410.0800.36655.60410.02000.897724.83

F103.0100.47970.65F103.0800.22923.37

-


3-255.1.2B1-221-1518.3200.56334.4710.0800.587818.66

pHist1 cl.217.5200.54545.19110.0800.59622.07

pHist4 cl.325.0200.50324.79210.0800.550220.38

pHist5 cl.33200.55015.24310.0800.654224.23

pHist6 cl.147.9200.54185.16410.0800.641723.77

F10F103100.15640.63F103400.21183.39

00

00

00

00

00

1, 50/ S, 50, 10 / 1 S, 1

00

00

00

00

00

00

1, 50 / S, 50, 10 / 1 S, 3,4 F10 1 S, 1

00

00

00

00

00

1, 50/ S, 50, 10 / 1 S, 24

00

00

00

00

00

00

1, 50 / S, 50, 10 / 1 S, 3,4 F10 1 S, 24

C, mg/g C, mg/g 280pNPh-b-Glu, U/mgCMC, U/mgAvicel, U/mgCellobiose, U/mg

pHist1 cl.27037422.4221.090.624.06

pHist4 cl.35296874.4315.470.797.58

pHist5 cl.36256930.9712.990.590.73

pHist6 cl.15896531.214.810.740.79

, # 87.6

1: 2 1

-3 2448,


3-255.1.2B1-221-151400.34952.121000.5797.361200.56828.7124

pHist1 cl.2400.59113.711200.49719.991600.440412.4048

pHist4 cl.3400.63483.891200.521810.301600.454412.63

pHist5 cl.3400.27612.431200.3598.301600.358411.06

pHist6 cl.1400.39592.921200.42529.111600.414311.97

-10.37170.0510.37170.0510.33050.05

-


3-255.1.2B1-221-151200.20671.58400.33415.12400.40466.20

pHist1 cl.2200.25262.17400.43957.55400.50518.67

pHist4 cl.3200.3042.61400.44267.60400.49138.43

pHist5 cl.3200.14251.22400.29695.10400.38556.62

pHist6 cl.1200.18081.55400.32345.55400.42327.27

-20.0360.0320.0340.0310.09260.04

1: 5 1

-3 24 48


3-255.1.2B1-221-151800.35574.281600.733814.002000.585114.83

pHist1 cl.2800.51486.811600.50813.502000.445415.60

pHist4 cl.3800.57197.271600.563514.412000.422415.13

pHist5 cl.3800.31155.151600.43112.242000.393914.55

pHist6 cl.1800.46166.371600.516313.642000.45415.78

-


3-255.1.2B1-221-151400.17412.67800.28058.60800.3049.32

pHist1 cl.2400.31975.49800.314210.79800.330811.36

pHist4 cl.3400.34385.90800.327611.25800.319810.98

pHist5 cl.3400.17453.00800.28729.86800.295510.15

pHist6 cl.1400.19573.36800.311710.70800.325311.17

1: 10 1

-3 24 48


3-255.1.2B1-221-1511600.22896.743200.236713.703200.29715.43

pHist1 cl.21600.313810.332000.548317.702000.578618.32

pHist4 cl.31600.326110.532000.552117.782000.56818.10

pHist5 cl.3800.59447.462000.466116.022000.563118.00

pHist6 cl.1800.71928.472000.551817.772000.586318.47

-


3-255.1.2B1-221-151800.16014.911600.169810.411600.184711.32

pHist1 cl.2400.46167.92800.400713.76800.396713.62

pHist4 cl.3400.47838.21800.388713.35800.389213.36

pHist5 cl.3400.28864.95800.364312.51800.393113.50

pHist6 cl.1400.32245.53800.38713.29800.39313.49

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00

00

00

00

00

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00

00

00

00

00

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pHist1 cl.2

pHist4 cl.3

pHist5 cl.3

pHist6 cl.1

,

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, 10 1

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B1-221-151

pHist1 cl.2

pHist4 cl.3

pHist5 cl.3

pHist6 cl.1

,

[], /

, 10 1

00

00

00

00

00

, 10 , 3

00

00

00

00

00

, 10 , 24

00

00

00

00

00

00

, 2 , 48

00

00

00

00

00

, 5 , 48

00

00

00

00

00

, 10 , 48

, # 87.6

1: 2 1

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3-255.1.2B1-221-151400.62083.091180.63149.241180.69879.9524

pHist1 cl.2800.25124.661600.413810.541600.473711.4148

pHist4 cl.3800.21224.341600.30729.011600.457711.18

pHist5 cl.3800.20524.281600.379110.051600.425910.72

pHist6 cl.1800.30625.101600.462711.251600.463111.25

-


3-255.1.2B1-221-151200.28912.22400.43746.73400.50017.66

pHist1 cl.2200.34892.99400.47538.16400.52198.96

pHist4 cl.3200.38123.27400.50998.75400.55679.56

pHist5 cl.3200.3693.17400.46237.94400.53779.23

pHist6 cl.1200.41913.60400.52449.00400.596210.24

1: 5 1

-3 24 48


3-255.1.2B1-221-151800.70246.771600.816115.182000.69916.87

pHist1 cl.21000.44687.811600.566914.462000.444715.59

pHist4 cl.31000.41067.441600.579214.662000.43915.47

pHist5 cl.31000.41547.491600.629715.492000.484216.39

pHist6 cl.11000.52878.651600.705516.722000.51717.06

-


3-255.1.2B1-221-151400.30884.75800.31019.54800.351610.78

pHist1 cl.2400.37916.51800.344711.84800.374512.86

pHist4 cl.3400.35396.08800.333511.45800.345211.85

pHist5 cl.3400.35586.11800.367412.61800.36712.60

pHist6 cl.1400.41687.16800.380213.05800.379113.02

1: 10 1

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3-255.1.2B1-221-1511600.58111.803200.321716.143200.348216.91

pHist1 cl.21600.364411.162000.597518.702000.620919.18

pHist4 cl.31600.349310.912000.510516.932000.52617.24

pHist5 cl.31600.371911.282000.591318.582000.641419.60

pHist6 cl.11600.401711.772000.642219.612000.642819.63

-


3-255.1.2B1-221-151800.23027.08800.366711.28800.384611.79

pHist1 cl.2400.50468.66800.401913.80800.420514.44

pHist4 cl.3400.49068.42800.361512.41800.374513.50

pHist5 cl.3400.50668.70800.410114.08800.441115.90

pHist6 cl.1400.57189.82800.428314.71800.428415.44

-3 24 48


F10 3-194.1.20,6 / 1 S100.10420.31100.68080.83200.50441.34

F10 3-194.1.21,7 / 1 S100.41750.59200.76341.80400.57582.93

F10 3-194.1.23 / 1 S200.33021.02400.64173.17800.44284.91

-


F10 3-194.1.20,6 / 1 S40.15050.2350.37930.73100.27141.04

F10 3-194.1.21,7 / 1 S50.2190.4250.80731.55100.55852.14

F10 3-194.1.23 / 1 S100.17540.67100.69942.69200.46093.53

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00

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, 5 1

00

00

00

00

00

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00

00

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pHist5 cl.3

pHist6 cl.1

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00

00

00

00

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00

00

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00

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00

00

00

00

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00

00

00

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000

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0,6 / 1 S

1,7 / 1 S

3 /1 S

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0,6 / 1 S

1,7 / 1 S

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00

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(IV) - ()

(IV) , pH5, 50, 72 0.2 /, 0.1 /Penicillium eTrichoderma :Spezyme CP,Multifect XL,CP + XL

Chart2

3.652173913

2.8695652174

1.6956521739

1.8260869565

2.1739130435

2.2608695652

2.17

1.64

0.98

0.3826086957

11.0909090909

4.4545454545

3.0909090909

1.5454545455

1.4090909091

1.08

1.1363636364

0.6363636364

0.2727272727

7.2727272727

1.1818181818

7.33

0.2434782609

Glucose (g/L)

Exp 1

Substate: Pretreated corn cob

Moisture of substrate: 65 %.

Yield ofYield of

1 h6 h244872016244872glucoseglucosePeakCglu, g/l

C. lucknowenseC. lucknowense

2C1 CBH Ia 650.50.260.650.50.37C1 CBH Ia 650.200.351.062.02.875.214.958542.8

3C1 CBH Ib 600.450.210.450.380.3C1 CBH Ib 600.170.250.691.52.264.111.762733.0

4C1 CBH IIb 700.570.310.640.410.28C1 CBH IIb 700.230.441.041.62.093.810.855752.6

5C1 EG II 510.640.360.750.490.34C1 EG II 510.260.531.242.02.614.713.678404.0

6C1 EG V 250.510.280.440.270.36C1 EG V 250.200.380.681.01.392.57.264283.1

7C1 EG VI 470.470.250.500.330.43C1 EG VI 470.180.330.791.31.703.18.855292.6

8C1 Xyl II 240.630.160.270.160.2C1 Xyl II 240.130.160.360.50.701.33.6

9C1 b-Glu 1060.820.230.300.190.23C1 b-Glu 1060.170.290.430.70.831.54.361373.0

T. reesei0.07T. reesei

10A1 CBH I 660.560.361.020.650.46A1 CBH I 660.220.531.732.73.656.619.0108655.7

11A1 CBH I 570.510.240.530.450.37A1 CBH I 570.200.310.841.82.875.214.963823.1

12A1 CBH II 600.60.330.590.350.43A1 CBH II 600.240.470.941.31.703.18.8

13A1 CBH II 500.670.350.610.40.25A1 CBH II 500.270.510.981.61.833.39.5

14A1 EG I hm0.540.330.710.440.29A1 EG I hm0.210.471.161.72.174.011.3

15A1 EG I lm0.720.410.730.430.3A1 EG I lm0.300.621.191.72.264.111.7

16A1 EG II hm0.70.340.600.350.22A1 EG II hm0.290.490.961.31.572.88.1

17A1 EG II lm0.590.30.450.240.16A1 EG II lm0.240.420.680.91.041.95.4

18A1 Xyl II 210.940.250.420.250.28A1 Xyl II 210.200.330.640.91.041.95.4

19substrate0.570.140.150.090.11substrate0.110.130.150.20.240.41.311090.0

20b-glu A. j.0.770.170.230.120.15b-glu A. j.0.160.180.290.30.380.72.0

14410

0.5

Yield ofYield of

16 h244872016244872sugarssugars

C. lucknowenseC. lucknowense% of dry S

2C1 CBH Ia 650.420.130.660.80.58C1 CBH Ia 650.270.301.472.13.25.88.899184.4045

3C1 CBH Ib 600.380.120.310.460.5C1 CBH Ib 600.250.290.901.42.95.27.9

4C1 CBH IIb 700.970.210.440.780.94C1 CBH IIb 700.490.371.112.12.34.26.4

5C1 EG II 511.60.420.980.470.7C1 EG II 510.750.541.992.93.76.710.1

6C1 EG V 2520.720.781.120.60C1 EG V 250.910.781.672.83.35.99.0

7C1 EG VI 471.110.20.440.61C1 EG VI 470.550.361.111.72.44.56.7

8C1 Xyl II 243.923.143.140.440.6C1 Xyl II 241.702.765.526.98.214.822.5

9C1 b-Glu 1060.660.250.280.400.50C1 b-Glu 1060.370.400.851.31.42.63.9

T. reeseiT. reesei

10A1 CBH I 660.870.31.220.710.91A1 CBH I 660.450.442.383.94.58.212.5

11A1 CBH I 570.430.110.470.280.93A1 CBH I 570.270.291.162.12.34.26.4

12A1 CBH II 600.60.10.273.061.3A1 CBH II 600.340.280.831.32.13.75.7

13A1 CBH II 500.880.110.360.620.95A1 CBH II 500.460.290.981.82.34.36.5

14A1 EG I hm1.050.331.183.020.7A1 EG I hm0.530.472.323.33.76.710.1

15A1 EG I lm1.2310.82.480.78A1 EG I lm0.601.011.702.84.07.311.0

16A1 EG II hm1.040.60.71.570.94A1 EG II hm0.520.691.531.82.34.26.4

17A1 EG II lm0.140.110.06511.2A1 EG II lm0.160.290.500.50.61.01.6

18A1 Xyl II 213.763.13.281.40.67A1 Xyl II 211.632.735.756.77.112.919.62.2805555556

19substrate0.120.10.0360.430.27substrate0.150.280.310.30.30.50.7

20b-glu A. j.0.140.110.030.780.86b-glu A. j.0.160.290.440.40.61.01.6

48162020

C. lucknowense

CBH Ia 651.40.91.41.11.1

CBH Ib 601.51.21.31.01.3

CBH Iib 702.20.81.11.31.1

EG II 512.91.01.61.51.4

EG V 254.62.12.52.82.3

EG VI 473.01.11.41.41.4

Xyl II 2413.316.915.213.311.7

b-Glu 1062.21.42.02.01.7

T. reesei

CBH I hm2.00.81.41.51.2

CBH I lm1.40.91.41.20.8

CBH II hm1.40.60.91.01.2

CBH II lm1.70.61.01.11.3

EG I hm2.51.02.01.91.7

EG I lm2.01.61.41.61.8

EG II hm1.81.41.61.41.5

EG II lm0.70.70.70.60.5

Xyl II8.38.39.07.36.8

substrate1.32.22.11.31.1

b-glu A. j.1.01.61.51.21.5

Exp 1

0000000000000000000

0000000000000000000

0000000000000000000

0000000000000000000

0000000000000000000

0000000000000000000

C1 CBH Ia 65

C1 CBH Ib 60

C1 CBH IIb 70

C1 EG II 51

C1 EG V 25

C1 EG VI 47

C1 Xyl II 24

C1 b-Glu 106

A1 CBH I 66

A1 CBH I 57

A1 CBH II 60

A1 CBH II 50

A1 EG I hm

A1 EG I lm

A1 EG II hm

A1 EG II lm

A1 Xyl II 21

substrate

b-glu A. j.

t, h

Cglu, g/l

Hydrolysis of pretreated corn cob.S - 50 g/l (dry), enzyme dosage - 0,1 g/l, cellulases with cellobiase 0,2 u/ml, 1 mM NaN3, pH 5, 50 0.

Exp 2

0000000000000000000

0000000000000000000

0000000000000000000

0000000000000000000

0000000000000000000

0000000000000000000

C1 CBH Ia 65

C1 CBH Ib 60

C1 CBH IIb 70

C1 EG II 51

C1 EG V 25

C1 EG VI 47

C1 Xyl II 24

C1 b-Glu 106

A1 CBH I 66

A1 CBH I 57

A1 CBH II 60

A1 CBH II 50

A1 EG I hm

A1 EG I lm

A1 EG II hm

A1 EG II lm

A1 Xyl II 21

substrate

b-glu A. j.

t, h

CRS, g/l


Total Bars

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

6 h

Cglu, g/l

Hydrolysis of pretreated corn cob with individual enzymes.Initial time - 6 hours

Total kinetics

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

72

Cglu, g/l


Xyl II

Xyl II

b-Glu C1

b-glu A. j.

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

6 h

CRS, g/l


b-glu A. j.

b-Glu C1

Xyl II

Xyl II

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

6 h

CRS, g/l


Xyl II

Xyl II

b-Glu C1

b-glu A. j.

Substate: Pretreated corn cob

Moisture of substrate: 65 %.

Yield ofYield of

1 h6 h244872016244872glucoseglucose

P. verruculosumP. verruculosum

1B1 CBH Ia 660.521.122.31.121.29B1 CBH Ia 660.411.915.079.411.0920.265.0

2B1 CBH I 550.340.490.820.420.56B1 CBH I 550.250.761.703.34.458.126.1

3B1 CBH IIb 600.330.460.820.320.41B1 CBH IIb 600.240.711.702.43.095.618.1

4B1 EG I 700.290.270.460.330.41B1 EG I 700.200.360.891.31.552.89.1

5B1 EG I 520.30.270.390.290.38B1 EG I 520.210.360.731.11.412.68.3

6B1 EG II 390.240.210.290.180.36B1 EG II 390.150.250.500.60.661.23.9

7B1 b-Glu 1060.460.851.650.770.91B1 b-Glu 1060.351.423.596.37.6413.944.8

P. canescensP. canescens

8a-L-AF 700.170.150.220.120.22a-L-AF 700.090.150.340.30.340.62.0

9a-L-AF 600.120.110.170.0950.18a-L-AF 600.050.070.230.20.250.51.5

10Xyl 10 A0.20.190.260.1450.27Xyl 10 A0.120.220.430.50.450.82.7

11a-Gal A0.170.140.220.120.23a-Gal A0.090.130.340.30.360.72.1

12C1 Ara 300.130.110.170.0950.17C1 Ara 300.050.070.230.20.230.41.3

13Pec0.150.120.200.120.22Pec0.070.090.300.30.340.62.0

14C1 EG III0.440.390.640.440.54C1 EG III0.340.581.301.72.143.912.5

15C1 Xyl II 240.180.170.270.180.34C1 Xyl II 240.100.180.450.60.611.13.6

16A1 Xyl II 210.270.250.380.250.27A1 Xyl II 210.180.330.700.90.911.75.3

17Spezyme 0,1 g/l0.490.781.600.730.87Spezyme 0,1 g/l0.381.293.486.07.2713.242.7

18Multifect 0,1 g/l0.280.310.470.170.33Multifect 0,1 g/l0.190.440.911.11.182.16.9

19S 0,07 g/l + M 0,025 g/l0.390.581.300.640.79S 0,07 g/l + M 0,025 g/l0.290.932.805.26.5511.938.4

24520

Yield ofYield of

1 h6 h244872016244872sugarssugars

P. verruculosumP. verruculosum% of dry S

1B1 CBH Ia 662.463.426.82.723.1B1 CBH Ia 661.093.007.3112.313.6424.837.6

2B1 CBH I 552.0623.181.131.32B1 CBH I 550.931.803.495.66.1511.216.9

3B1 CBH IIb 600.70.911.671.051.42B1 CBH IIb 600.350.881.912.63.286.09.0

4B1 EG I 700.650.591.170.751.04B1 EG I 700.330.611.382.02.484.56.8

5B1 EG I 520.720.50.910.700.85B1 EG I 520.360.541.111.92.083.85.7

6B1 EG II 390.30.10.150.660.34B1 EG II 390.190.200.310.50.510.91.4

7B1 b-Glu 1062.422.965.281.502.06B1 b-Glu 1061.082.615.718.99.2616.825.5

P. canescensP. canescens

8a-L-AF 700.310.270.190.570.3a-L-AF 700.190.350.350.40.460.81.3

9a-L-AF 600.10.090.100.450.22a-L-AF 600.100.190.250.30.380.71.0

10Xyl 10 A0.290.470.651.151.28Xyl 10 A0.180.510.831.41.492.74.1

11a-Gal A0.130.110.120.440.17a-Gal A0.110.210.270.30.330.60.9

12C1 Ara 302.11.482.960.982.3C1 Ara 300.941.363.265.05.149.314.2

13Pec0.120.070.110.330.43Pec0.110.180.260.30.300.50.8

14C1 EG III1.261.111.440.851.03C1 EG III0.591.051.662.22.464.56.8

15C1 Xyl II 243.844.084.901.341.59C1 Xyl II 241.683.555.316.57.2813.220.1

16A1 Xyl II 213.643.023.861.081.34A1 Xyl II 211.592.664.215.46.2311.317.2

17Spezyme 0,1 g/l3.63.887.042.562.82Spezyme 0,1 g/l1.573.397.5611.612.4622.734.3

18Multifect 0,1 g/l3.83.645.961.792.3Multifect 0,1 g/l1.663.186.428.410.2718.728.3

19S 0,07 g/l + M 0,025 g/l3.763.966.962.583.06S 0,07 g/l + M 0,025 g/l1.643.457.4711.713.4724.537.1

481020

P. verruculosum

CBH Ia 662.71.61.41.31.2

CBH I 553.82.42.11.71.4

CBH IIb 601.51.21.11.11.1

EG I 701.71.71.61.61.6

EG I 521.71.51.51.71.5

EG II 391.20.80.60.70.8

b-Glu 1063.01.81.61.41.2

P. canescens

a-L-AF 702.12.41.01.21.4

a-L-AF 602.22.71.11.41.5

Xyl 10 A1.52.41.93.13.3

a-Gal A1.31.70.81.00.9

Ara 30 C117.318.814.420.822.6

Pec1.51.90.90.80.9

EG III C11.81.81.31.31.2

Xyl II C116.819.511.710.711.9

Xyl II A18.88.16.05.96.9

Spezyme 0,1 g/l4.12.62.21.91.7

Multifect 0,1 g/l8.77.37.17.48.7

S 0,07 g/l + M 0,025 g/l5.63.72.72.22.1

0000000000000000000

0000000000000000000

0000000000000000000

0000000000000000000

0000000000000000000

0000000000000000000

B1 CBH Ia 66

B1 CBH I 55

B1 CBH IIb 60

B1 EG I 70

B1 EG I 52

B1 EG II 39

B1 b-Glu 106

a-L-AF 70

a-L-AF 60

Xyl 10 A

a-Gal A

C1 Ara 30

Pec

C1 EG III

C1 Xyl II 24

A1 Xyl II 21

Spezyme 0,1 g/l

Multifect 0,1 g/l

S 0,07 g/l + M 0,025 g/l

t, h

Cglu, g/l


0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

6 h

Cglu, g/l


0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

6 h

CRS, g/l


0000000000000000000

0000000000000000000

0000000000000000000

0000000000000000000

0000000000000000000

0000000000000000000

B1 CBH Ia 66

B1 CBH I 55

B1 CBH IIb 60

B1 EG I 70

B1 EG I 52

B1 EG II 39

B1 b-Glu 106

a-L-AF 70

a-L-AF 60

Xyl 10 A

a-Gal A

C1 Ara 30

Pec

C1 EG III

C1 Xyl II 24

A1 Xyl II 21

Spezyme 0,1 g/l

Multifect 0,1 g/l

S 0,07 g/l + M 0,025 g/l

t, h

CRS, g/l


0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

6 h

Cglu, g/l


0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

6 h

CRS, g/l


Cglu, g/lCglu, g/l

6 h72 h

Tr CBH I 660.533.65

Tr CBH I 570.312.87

Tr CBH II 600.471.70

Tr CBH II 500.511.83

Tr EG I hm0.472.17

Tr EG I lm0.622.26

Tr EG II hm0.672.17

Tr EG II lm0.571.64

Tr Xyl II 210.330.98

A.j. BGL0.180.38

Pv CBH I 66 (A280)1.9111.09

Pv CBH I 550.764.45

Pv CBH II 600.713.09

Pv EG I 700.361.55

Pv EG I 520.361.41

Pv EG II 390.371.08

Pv EG III 250.671.14

Pv BGL 1160.470.64

Pv Xyl 230.220.27

Spezyme 0,1 g/l1.297.27

Multifect 0,1 g/l0.441.18

S 0,07 g/l + M 0,025 g/l1.157.33

Background substrate0.130.24

RS - Glu (g/l)RS - Glu (%)

6 h72 h72 h72 h

C1 CBH Ia 650.533.343.34100

C1 CBH Ib 600.362.632.63100

C1 CBH IIb 700.602.752.75100

C1 EG II 510.543.673.67100

C1 EG III 281.052.462.46100

C1 EG V 250.783.273.27100

C1 EG VI 470.362.452.45100

C1 Xyl II 243.167.727.72100

C1 AraGal 302.065.685.68100

C1 BGL 1060.401.431.43100

A1 CBH I 660.444.530.8819

A1 CBH I 570.292.31-0.56-24

A1 CBH II 600.282.050.3617

A1 CBH II 500.292.350.5222

A1 EG I hm0.473.671.5041

A1 EG I lm1.014.001.7443

A1 EG II hm0.662.850.6824

A1 EG II lm0.331.52-0.12-8

A1 Xyl II 212.706.675.6985

A.j. BGL0.290.580.1934

B1 CBH I 66 (A280)3.0013.642.5519

B1 CBH I 551.806.151.6928

B1 CBH II 600.883.280.196

B1 EG I 700.612.480.9438

B1 EG I 520.542.080.6832

B1 EG II 390.421.270.1915

B1 EG III 250.631.00-0.14-14

B1 BGL 1160.440.63-0.00-1

B1 Xyl 232.863.933.6693

P.c. AF 700.350.460.46100

P.c. AF 600.190.380.38100

P.c. a-Gal0.210.330.33100

P.c. Pec0.180.300.30100

Spezyme 0,1 g/l3.3912.465.1942

Multifect 0,1 g/l3.1810.279.0988

S 0,07 g/l + M 0,025 g/l4.5014.086.7548

Background substrate0.280.260.015

Glucose (g/L)

Reducing sugars (g/L)

Hydrolysis of pretreated corn cobs for 72 hours.S - 50 g/l (dry), enzyme dosage - 0,1 g/l, cellulases with cellobiase 0,2 U/ml, 1 mM NaN3, pH 5, 50 0.

00000000000

0.19545454550.17272727270.22727272730.25909090910.20.18181818180.33636363640.12727272730.10.17045454550.0545454545

0.34545454550.25454545450.43636363640.52727272730.38181818180.32727272730.58181818180.16363636360.18181818180.29090909090.0727272727

1.05612648220.69249011861.03913043481.23517786560.67549407110.79011857711.29545454550.36403162060.45454545450.42687747040.2272727273

21.47826086961.60869565221.956521739111.26086956521.73913043480.52173913040.60869565220.6521739130.2391304348

2.86956521742.26086956522.08695652172.60869565221.39130434781.69565217392.13636363640.69565217390.61363636360.82608695650.2272727273

C1 CBH Ia 65

C1 CBH Ib 60

C1 CBH IIb 70

C1 EG II 51

C1 EG V 25

C1 EG VI 47

C1 EG III

C1 Xyl II 24

C1 Xyl II 24

C1 b-Glu 106

C1 Ara 30

t, h

Cglu, g/l


0000000

0.40909090910.24545454550.23636363640.20.20909090910.15454545450.3545454545

1.90909090910.76363636360.70909090910.36363636360.36363636360.25454545451.4181818182

5.06818181821.70454545451.70454545450.88636363640.72727272730.53.5909090909

9.39130434783.30434782612.43478260871.26086956521.08695652170.60869565226.347826087

11.09090909094.45454545453.09090909091.54545454551.40909090910.65909090917.6363636364

B1 CBH Ia 66

B1 CBH I 55

B1 CBH IIb 60

B1 EG I 70

B1 EG I 52

B1 EG II 39

B1 b-Glu 106

t, h

Cglu, g/l


00000

0.11363636360.15909090910.38181818180.19090909090.2909090909

0.12727272730.18181818181.29090909090.43636363640.9272727273

0.14545454550.29288537553.47727272730.90909090912.7954545455

0.21739130430.34782608761.13043478265.2173913043

0.24347826090.38260869577.27272727271.18181818186.5454545455

substrate

b-glu A. j.

Spezyme 0,1 g/l

Multifect 0,1 g/l

S 0,07 g/l + M 0,025 g/l

t, h

Cglu, g/l


0000000000

0.22272727270.20.24090909090.27272727270.21363636360.29545454550.28636363640.23636363640.19772727270.1818181818

0.52727272730.30909090910.47272727270.50909090910.47272727270.61818181820.49090909090.41818181820.32727272730.3272727273

1.73162055340.84387351780.94071146250.98181818181.16324110671.19090909090.95810276680.68260869570.63833992090.7045454545

2.6521739131.78260869571.3478260871.56521739131.73913043481.69565217391.3478260870.86956521740.91304347830.9130434783

3.6521739132.86956521741.69565217391.82608695652.17391304352.26086956521.56521739131.04347826091.04347826090.9090909091

A1 CBH I 66

A1 CBH I 57

A1 CBH II 60

A1 CBH II 50

A1 EG I hm

A1 EG I lm

A1 EG II hm

A1 EG II lm

A1 Xyl II 21

A1 Xyl II 21

t, h

Cglu, g/l


00000

0.09090909090.04545454550.11818181820.09090909090.0727272727

0.14545454550.07272727270.21818181820.12727272730.0909090909

0.34090909090.22727272730.43181818180.34090909090.2954545455

0.3478260870.23913043480.45652173910.3478260870.347826087

0.34090909090.250.45454545450.36363636360.3409090909

a-L-AF 70

a-L-AF 60

Xyl 10 A

a-Gal A

Pec

t, h

Cglu, g/l


00000000000

0.26938775510.25306122450.4938775510.75102040820.91428571430.55102040820.58947368421.69795918371.67578947370.36734693880.9431578947

0.30204081630.2938775510.36734693880.53877551020.78367346940.35918367351.05263157892.75918367353.55368421050.41.3642105263

1.46938775510.89795918371.11020408161.99183673471.66530612241.11020408161.66315789475.51836734695.30526315790.84897959183.2631578947

2.12244897961.42857142862.08163265312.89795918372.77551020411.71428571432.21052631586.93877551026.48421052631.3061224494.9684210526

3.18367346942.85714285712.32653061223.67346938783.26530612242.44897959182.46315789478.16326530617.28421052631.42857142865.1368421053

C1 CBH Ia 65

C1 CBH Ib 60

C1 CBH IIb 70

C1 EG II 51

C1 EG V 25

C1 EG VI 47

C1 EG III

C1 Xyl II 24

C1 Xyl II 24

C1 b-Glu 106

C1 Ara 30

t, h

CRS, g/l


0000000

1.09473684210.92631578950.35368421050.33263157890.36210526320.18526315791.0778947368

2.99789473681.80210526320.88421052630.61473684210.53894736840.20210526322.6105263158

7.30526315793.49473684211.90526315791.37894736841.10526315790.30526315795.7052631579

12.29473684215.62.631578947421.89473684210.45263157898.9473684211

13.64210526326.14736842113.28421052632.48421052632.08421052630.50526315799.2631578947

B1 CBH Ia 66

B1 CBH I 55

B1 CBH IIb 60

B1 EG I 70

B1 EG I 52

B1 EG II 39

B1 b-Glu 106

t, h

CRS, g/l


0000000000

0.45306122450.27346938780.34285714290.45714285710.52653061220.60.52244897960.15510204081.63265306121.5915789474

0.44081632650.28571428570.27755102040.28571428570.46530612241.0122448980.68571428570.28571428572.72653061222.6610526316

2.38367346941.15918367350.83265306120.97959183672.31836734691.69795918371.53469387760.49795918375.74693877554.2105263158

3.87755102042.12244897961.34693877551.75510204083.32653061222.77551020411.84693877550.5061224496.6938775515.3894736842

4.53061224492.3061224492.05102040822.34693877553.673469387842.32653061220.57142857147.10204081636.2315789474

A1 CBH I 66

A1 CBH I 57

A1 CBH II 60

A1 CBH II 50

A1 EG I hm

A1 EG I lm

A1 EG II hm

A1 EG II lm

A1 Xyl II 21

A1 Xyl II 21

t, h

CRS, g/l


00000

0.18947368420.10105263160.18105263160.11368421050.1094736842

0.34526315790.19368421050.51368421050.21052631580.1768421053

0.34736842110.25263157890.83157894740.27368421050.2631578947

0.40526315790.34210526321.42105263160.33684210530.2789473684

0.46315789470.37894736841.49473684210.32631578950.3

a-L-AF 70

a-L-AF 60

Xyl 10 A

a-Gal A

Pec

t, h

CRS, g/l


00000

0.14693877550.15510204081.57473684211.65894736841.6421052632

0.27755102040.28571428573.38526315793.18315789473.4526315789

0.310.44081632657.55789473686.42105263167.4736842105

0.27346938780.416326530611.62105263168.378947368411.7052631579

0.25578231290.576870748312.463157894710.273684210513.4736842105

substrate

b-glu A. j.

Spezyme 0,1 g/l

Multifect 0,1 g/l

S 0,07 g/l + M 0,025 g/l

t, h

CRS, g/l


2 h6 h2448729602624487296


1CBH Ia 65 kDaCBH Ia 65 kDa

2CBH Ia 65 kDa0.50.260.650.50.370.43CBH Ia 65 kDa0.100.000.000.02.870.0

3CBH Ib 60 kDa0.450.210.450.380.30.38CBH Ib 60 kDa0.090.000.000.02.260.0

4CBH IIb 70 kDa0.570.310.640.410.280.33CBH IIb 70 kDa0.110.000.000.02.090.0

5EG II 51 kDa0.640.360.750.490.340.4EG II 51 kDa0.130.000.000.02.610.0

6EG V 25 kDa0.510.280.440.270.360.25EG V 25 kDa0.100.000.000.01.390.0

7EG VI 47 kDa0.470.250.500.330.430.29EG VI 47 kDa0.090.000.000.01.700.0

8Xyl II 24 kDa0.630.160.270.160.20.42Xyl II 24 kDa0.000.000.000.00.700.8

9b-Glu 106 kDa0.820.230.300.190.230.17b-Glu 106 kDa0.000.000.000.00.830.0

T. reesei0.07T. reesei

10CBH I hm0.560.361.020.650.460.59CBH I hm0.110.000.000.03.650.0

11CBH I lm0.510.240.530.450.370.41CBH I lm0.100.000.000.02.870.0

12CBH II hm0.60.330.590.350.430.28CBH II hm0.120.000.000.01.700.0

13CBH II lm0.670.350.610.40.250.29CBH II lm0.140.000.000.01.830.0

14EG I hm0.540.330.710.440.290.35EG I hm0.110.000.000.02.170.0

15EG I lm0.720.410.730.430.30.35EG I lm0.150.000.000.02.260.0

16EG II hm0.70.340.600.350.220.26EG II hm0.140.000.000.01.570.0

17EG II lm0.590.30.450.240.160.2EG II lm0.120.000.000.01.040.0

18Xyl II0.940.250.420.250.280.2Xyl II0.000.000.000.01.040.0

19substrate0.570.140.150.090.110.15substrate0.000.000.000.00.240.2

20b-glu A. j.0.770.170.230.120.150.23b-glu A. j.0.000.000.000.00.380.4

1441020

0.5

26 h2448729602624487296


1CBH Ia 65 kDaCBH Ia 65 kDa

2CBH Ia 65 kDa0.420.130.660.80.580.34CBH Ia 65 kDa0.000.000.000.00.00.0

3CBH Ib 60 kDa0.380.120.310.460.50.28CBH Ib 60 kDa0.000.000.000.00.00.0

4CBH IIb 70 kDa0.970.210.440.780.940.18CBH IIb 70 kDa0.000.000.000.00.00.0

5EG II 51 kDa1.60.420.980.470.70.44EG II 51 kDa0.000.000.000.00.00.0

6EG V 25 kDa20.720.781.120.600.34EG V 25 kDa0.000.000.000.00.00.0

7EG VI 47 kDa1.110.20.440.610.25EG VI 47 kDa0.000.000.000.00.00.0

8Xyl II 24 kDa3.923.143.140.440.61.5Xyl II 24 kDa0.000.000.000.00.00.0

9b-Glu 106 kDa0.660.250.280.400.500.07b-Glu 106 kDa0.000.000.000.00.00.0

T. reeseiT. reesei

10CBH I hm0.870.31.220.710.910.71CBH I hm0.000.000.000.00.00.0

11CBH I lm0.430.110.470.280.930.2CBH I lm0.000.000.000.00.00.0

12CBH II hm0.60.10.273.061.30.16CBH II hm0.000.000.000.02.10.0

13CBH II lm0.880.110.360.620.950.18CBH II lm0.000.000.000.00.00.0

14EG I hm1.050.331.183.020.70.45EG I hm0.000.000.000.00.00.0

15EG I lm1.2310.82.480.780.56EG I lm0.000.000.000.00.00.0

16EG II hm1.040.60.71.570.940.2EG II hm0.000.000.000.00.00.0

17EG II lm0.140.110.06511.20.2EG II lm0.000.000.000.00.00.7

18Xyl II3.763.13.281.40.671.11Xyl II0.000.000.000.00.00.0

19substrate0.120.10.0360.430.270.09substrate0.000.000.310.00.30.3

20b-glu A. j.0.140.110.030.780.860.2b-glu A. j.0.000.000.000.00.60.5

- () (IV) - Penicillium 537 (CBH1 ) -GCBH-s

(.)The Chemical Journal, .2008, .39

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ЭНЗИМАТИЧЕСКИЕ МЕТОДЫ КОНВЕРСИИ БИОМАССЫ: СОВРЕМЕННОЕ СОСТОЯНИЕ