Upload
oral
View
90
Download
9
Embed Size (px)
DESCRIPTION
猪基因组信息资源的 整合与利用. 亓小红 天津农学院. 猪是人类最早驯化的家畜之一,作为世界的一个重要畜种,在地球上的分布很广。 世界范围内的猪肉消费量占红肉消费量的 43%(Rothscild and Ruvinsky,1998) 。 在我国肉类消费中,猪肉所占比例 67%(FAO, 2001) 。 研究人类疾病理想的实验动物模型和人类器官移植的供体。. 育种任务. 胴体品质好 肉质好 生长快 饲料消耗低 繁殖率高 抗病. 群体遗传学和数量遗传学理论的指导。 - PowerPoint PPT Presentation
Citation preview
43%(Rothscild and Ruvinsky,1998)67%(FAO, 2001)
BLUP
(Linkage Map (Physical Map QTLPigQTLdb(Comparative Map)ESTSNP
Linkage MapsArchibald ,1995; Rohrer,1996AFLPSNP 5000
Loci / Markers Publications / AuthorsClones Libraries Database Statistics
SW2073Primer Information Forward primer:ATTTGCATAATAGCCTTATCCCTAC Reverse primer:CAATTTTATATGCACAGATTTATTTAAAGPCR product size:133-148 (bp), Sus scrofaGenBank Accession:AF253788
Physical Maps4000EST
Drawings of genes and markers mapped on pig chromosome List of genes mapped on pig map List of genetic markers mapped on pig map
Comparative mapsThe Comparative Map: Pigs and Humans INRA(Inland Northwest Research Alliance ) The Comparative Map: Pigs and Mouse - Japan The Mammalian Comparative Map - Jackson Lab The HomoloGene Database - NCBI
EST98988cDNA,.DNA.
SNP(NEW)AlleleChromosomeBase PositionFunctionGene NameProtein Heterozygosity
QTL-- PigQTLdbQTLQTLQTL10QTL862351129QTL
QTLQTLQTL
QTL on Pig Chromosome 1
Legend: Red QTL lines represent for significant and light blue QTL lines for suggestive levels. Green dots shows the position of QTL peaks. QTL\
Number of QTL by chromosomesClick on each link to go to each chromosome for QTL on it.
Chromsome QTLs FoundX471150211335041865366141715386992510211119122413441448153116917181815
Number of QTL by trait typesClick on each trait name to find QTL for it
Trait TypesQTLs FoundAnatomy367Behavioral22Chemical11Coat_color2Conductivity22Conformation8Digestive Organ9Disease Resistance6Endocrine4Enzyme Activity1Fat Composition40Fatness302Feed Conversion5Feed Intake11Flavor12Growth184Immune Capacity8Litter Size20Meat_color41Odor5PH38Reproductive Organ28Reproductive Traits9Stiffening3Texture40
Number of QTL by trait classesClick on each trait class assiciated QTL
Trait ClassQTLs FoundExterior32Health14Meat Quality882Production209Reproduction61
QTLQTLLD
QTLQTN MAS
1the porcine pituitary-specific transcription factor PIT-1) NAminopeptidase NAMPEPN 4melanocortin 4 receptor MC4R)(Kim et al. 2000)
HALRNRNpH15DNARNRNAMPAMP-activated protein kinasesPRKA) adipocyte fatty acid-binding protein (A-FABP) IGF2
ESR1.15 0.42retinoic acid receptor gammaRARG) 1Amelatonin receptor 1A MTNRIA) follicle stimulating hormone beta FSHB) prolactin receptor PRLR) 4retinol binding protein 4RBP4) phosphoglycerate kinase 2 PGK2)
KIT ( ) 1melanocortin 4 receptor MC1R),
NRAMP 12-(FUT1FUT2)F18K88acR133E. coli F4ab/acSLA
2n=38310-9bpDNAcDNA)ESTEST
NorthernDDRT-PCRPCR
MAS (GAS)QTL MSEBV MSEBVI=b1MS+b2EBV
Plastow (2003)15
,,MAS,
,
QTL
MAS
!
!