GCM 2.0 type strain sequencing project workshop · Сэдэв: Монгол орны бичил биетний генетик нөөцийг ex-situ хамгаалах, ашиглах

GCM 2.0 type strain sequencing project workshop

Enkh-Amgalan J.

Laboratory of Microbiology

Institute of General and Experimental Biology, MAS

Landlocked

High altitude (~1580m)

The temperature fluctuates

throughout the year: - 40℃ to 38℃

Mongolia has unique geography, environment and climate

condition which are resulted in unique diversity of plants and

animals containing many species endemic to Mongolia. Thus

we expect unique diversity and novel taxa of microorganisms

in Mongolia as well as microbes of economically importance.

Institute of General and Experimental Biology, MAS

The Institute of Biology established in

1965 was merged with the Institute

of Botany in 2015 and was named

thereafter as the Institute of General

and Experimental Biology (IGEB).

Mission: Study of the biological

resources and biodiversity of

Mongolian ecosystems,

development of scientific

justification for conservation and

sustainable use of bio-resources and

application of research results into

practice to achieve the

sustainability.

Publishes “Proceedings of the

Institute of General and

Experimental Biology”

Structure 1. Laboratory of Genetics

2. Laboratory of Molecular biology

3. Laboratory of Microbiology 4. Laboratory of Microbial synthesis

5. Laboratory of Plant biotechnology

6. Laboratory of Flora and Plant

systematics

7. Laboratory of Vegetation ecology

and Plant resource

8. Laboratory of Anatomy and Eco-

Physiology

9. Laboratory of Forest Phytocenology

10. Laboratory of Plant introduction

11. Laboratory of Mammalian ecology

12. Laboratory of Ornithology

13. Laboratory of Entomology

14. Laboratory of Hydrobiology and

Ichthyology

15. Research center for Fish breeding

16. Botanical garden

Laboratory of Microbiology, IGEB-MAS Laboratory of Microbiology, IGEB-MAS

IGEB-MAS – GCM 2.0, Beijing, Nov 21-22, 2018

Our laboratory was established in 1985 as the Laboratory of microbial

physiology and biochemistry.

Laboratory of Microbiology, IGEB-MAS

Mission:

Research and generation of

new knowledge on

Mongolian microbial

diversity and their genetic

resources, ex-situ

conservation of microbial

diversity.

Vision:

Wide application of

microorganisms in different

branches of national

economy and

environmental protection for

benefits of the present and

future generations.


Current Status of Culture Collection



Culture collection

In 2018 within the domestic research

project we isolated and enriched

the collection with 285 strains of

lactic acid bacteria, fungi & yeast.

~ 9000 microbial cultures

~ 8000 isolated ~ 5600 identified

within the MAS-NITE joint research project (2006-2017)

Actinomycetes

Bacteria

26%

Filamentous

fungi

23%

Yeast

6%

Archaea

1%

Chromista

1%

Identified strains by using molecular markers

Actinomycetes Bacteria Filamentous fungi

Yeast Archaea Chromista

Strains Genera

■Actinomycetes 2443 66

■Bacteria 1437 81

■Filamentous fungi 1275 178

■Yeast 321 22

■Archaea 55 5

■Chromista 73 6

5604 358



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Mongolia-Japan joint research project (2006-2017) titled

“Taxonomic and Ecological Studies of Microorganisms in

Mongolia and the Utilization” implemented successfully. The Final

Meeting of the Joint Research Project was held on Aug 08, 2016

in Ulaanbaatar.

MAS of Mongolia and NITE of Japan signed MOU and PA on Joint

Research Program on “Transfer, Utilization and Benefit-Sharing of

Microbial Resources” (2017-2022) on March 31, 2017.

The Final Meeting of the Joint Research Project, Aug 08, 2016 in Ulaanbaatar


Type strains



Strain number Other collection numbers Genus_Name Species_Epithet

MN07-A0368 NBRC 105298 Actinoplanes toevensis

MN07-A0371 NBRC 105297 Actinoplanes tereljensis

MN07-A0370 NBRC 105296 Luteipulveratus mongoliensis

MN08-A0270 NBRC 105885 Pseudonocardia mongoliensis

MN08-A0297 NBRC 105886 Pseudonocardia khuvsgulensis

MN08-A0203 NBRC 105883 Actinophytocola burenkhanensis

MN08-A0264 NBRC 105887 Cryptosporangium mongoliense

MN08-A0118 NBRC 105882 Herbidospora mongoliensis

Many new species candidates

Proposal of two novel species of the genus

Phyllobacterium


One researcher of the

laboratory participated in

“The 8th international

training course on

microbial taxonomy” held

for 6 weeks in Korean

Collection for Type

Cultures (KCTC), Korea.

A proposal of two novel species of the genus Phyllobacterium is

under preparation. The two strains were deposited in KCTC.

Phyllobacterium sp. 30N-2 / KCTC 62248

Phyllobacterium sp. 30N-3 / KCTC 62249


8 June

2018

12

-800C



Preservation of microbial strains

Research Activities



Ecology and diversity of microorganisms in Mongolian

natural environments (soils, hot springs, saline lakes,

permafrost) and dairy products;

Genetic resources of microorganisms (antimicrobials,

enzymes and biosynthetic genes);

Plant-microbe interactions:

- endophytic microorganisms of Mongolian plants and

their bio-active compounds; - nitrogen-fixing bacteria;

- rhizosphere actinomycetes and bacteria;

Isolation, ex-situ conservation and taxonomic

identification of microorganisms;

Research topics



Taxonomic diversity of Mongolian

microorganisms Group Number of genera

known in 1973 when the

Department of

Microbiology was established in MAS

Number of genera known in 2016

Actinomycetes 1 92

Other Bacteria 2 81

Archaea 0 5

Fungi microscopic

27 200

Chromista 0 6


Diversity of prokaryotic

microorganisms Kingdom Class Order Family Genera

Bacteria 1. Actinobacteria 9 24 65

2. Thermoleophilia 1 1 1

3. Alphaproteobacteria 5 13 25

4. Betaproteobacteria 2 3 13

5. Gammaproteobacteria 6 7 17

6. Bacilli 2 10 24

7. Flavobacteriia 1 1 2

Archaea Halobacteria 2 2 5


Diversity of eukaryotic microorganisms

Kingdom Class Order Family Genus

Fungi 1. Dothideomycetes 6 23 60

2. Eurotiomycetes 4 6 13

3. Leotiomycetes 2 8 15

4. Pezizomycetes 1 3 4

5. Saccharomycetes 2 8 19

6. Sordariomycetes 9 24 58

7. Agaricomycetes 6 9 11

8. Microbotryomycetes 1 1 1

9. Tremellomycetes 2 2 2

10. Ustilaginomycetes 2 2 2

11. Chytridiomycetes 2 2 2

Incertae sedis 3 7 13

Chromista Labyrinthulomycetes 1 1 2

Oomycetes 3 3 4


- ground, including rock or (cryotic) soil, at or below 0°C

for 2 or more years. Permafrost underlies about 63% of Mongolia.

Site Depth Filamentous fungi Yeast

0℃ 4℃ 15℃ 0℃

5m 2 1 3 7

7m 3 3 2 6

9m - - - 2

7m 1 2 5 -

10m - - - -


Psychrophilic yeast

AF444709_Cystofilobasidium ferigula_CBS6954

AF363647_C.victoriae_CBS8685

35_O7

KC442255_C.carnescens_YM25350

25_U5

100

88

AF137606_C.albidosimilis_ATCC

27_O7

KC442268_C.albidus_YM25370

30_U5

33_U5

66

AF137603_C.adeliensis_CBS8351

37_O7

29_O7

26_O7

24_U5

100

100

100

82

100

100

0.02

26S rRNA gene D1/D2

(Cryptococcus)

They were closely related to representatives of Cryptococcus

and Cystofilobasidium, isolated from Antarctic permafrost soils.

KF891469_Cryptococcus_adeliensis_A1

AF444709_C.ferigula_CBS6954

KP346924_C.capitatum_CBS13730

AF189832_C.bisporidii_CBS6346

AB259926_C.infirmominiatum_RY-29

23_U5

36_U5

28_O9

31_O7

32_U5

34_O7

AF189848_C.macerans_CBS2206

56

99

98

96

66

67

0.02

New species?

26S rRNA gene D1/D2

(Cystofilobasidium)

Human resource development



Workshop on Identification of Mitosporic Fungi by K. Ado, 10-11 August 2013

Workshop on “Introduction of Molecular Phylogenetic Analysis of Microorganisms” by S.

Sekimoto and on “Identification of Mitosporic Fungi” by K. Ando, 21-23 August, 2014

Workshop on Identification of Mitosporic Fungi by K. Ando and Diagnosis of Plant Diseases caused by Fungi by K. Watanabe, 8-9 August 2015

Workshop on Identification of Mitosporic Fungi by K. Ando, 6-7 August 2016

Human resource development in microbial identification

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IGEB-MAS - ACM14, Taipei, Dec 04-06, 2017


Workshop on Identification of Mitosporic Fungi by K. Ando, 8-9 November 2018



More than 80 scientists from 20

research organizations in 12 countries.



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IGEB-MAS - ACM14, Taipei, Dec 04-06, 2017

Documents

GCM 2.0 type strain sequencing project workshop · Сэдэв: Монгол орны бичил биетний генетик нөөцийг ex-situ хамгаалах, ашиглах