Manual Practicas Lab Bq

7/24/2019 Manual Practicas Lab Bq

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Presentacin del manual ................................................................................................. 2

Seguridad en el laboratorio de Bioqumica ................................................................... 4

Reactivos en el laboratorio de Bioqumica .................................................................... 5

Equipos en el laboratorio de Bioqumica ....................................................................... 8

Modalidades de conduccin del proceso de enseanza-aprendizaje .................. 13

Prctica 1. Purificacin de lactato deshidrogenasa (LDH) ........................................ 14

Prctica 2. Medicin de actividad enzimtica de la LDH .......................................... 23

Prctica 3. Cuantificacin de protenas por el mtodo de Bradford ........................ 29

Prctica 4. Electroforesis en geles de poliacrilamida desnaturalizantes ................. 35

Prctica 5. Cromatografa de intercambio inico ...................................................... 45

Prctica 6. Cromatografa de afinidad ........................................................................ 50

Prctica 7. Cintica enzimtica .................................................................................... 55

Bibliografa ....................................................................................................................... 59

Anexo A. Rbricas y lista de cotejo .............................................................................. 61

Anexo B. Tabla de sulfato de amonio ......................................................................... 64

Anexo C. Propuestas de organizacin de las prcticas en el trimestre .................. 65


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