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第九章 固有免疫的组成 细胞及固有免疫应答

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第九章 固有免疫的组成 细胞及固有免疫应答. 免疫分为 非特异性或天然免疫 ( Non-specific or innate immunity  ) 特异性或获得性免疫 ( Specific or adaptive immunity ). The differences between the two types of immunity. 天然免疫. 获得性免疫. 抗原非依赖性( antigen-independent ). 抗原依赖性( antigen-dependent ). 立即( immediate )达到最大反应. - PowerPoint PPT Presentation

Text of 第九章 固有免疫的组成 细胞及固有免疫应答

  • Non-specific or innate immunity Specific or adaptive immunity antigen-independent immediate antigen-dependent lag time patternMacromoleculesThe differences between the two types of immunity

  • NKNKTB1T1 (neutrophils)2 (macrophages)

    TB

  • NK

  • monocyte (macrophage, M)

  • (

  • A

  • .pattern recognition receptor,PRR)pathogen associated molecular pattern, PAMP):

  • scavenger receptorSRToll

  • CLCLCLCLCLCLCLCLF2 NH2 CRDCOOH mannose receptorMR180 kDa8

  • C SSCOOH COOHHOOCNH2NH2 2HNS scavenger receptorSR(scavenger receptor)

  • TollLBP ()sCD14Soluble CD14LPS

  • 2.complement receptor, CRFcR

    IgGC3b

  • CD16 FcR IIICD11b/CD18 CD25CD28 CD32 Fc R II CD35 CR1 CD64 Fc R ICD71B7-2IL-2

  • Adherence of bacteria via receptors

  • A --- H2O2O2-NO B --- C ---

  • pattern

  • NADPH H+e- + O2O2-H+H2O2b558

    II (NADPH) b558

  • O2 + NADPH O2- + NADP + H+

    2O2 + 2H2 H2O2 + O2

    H2O2 + Cl + H+ HOCl + H2O

    HOCl + R-NH RNCl + H2O

    H2O2 + OCl- 1 O2 + H2O + Cl-

    H2O2 + O2- O2 + OH- + .OH NADPH

  • NO

    NO

    L- + 1/2O2 + NO

    NO + O2 - ONOO-

  • Oxygen-independent mechanisms of intracellular killingCationic ( )

  • Macrophage Phagocytosis

  • ADCC

  • Th1

  • --- (IL-1IL-6IL-8IL-12TNF-)-IL-1/12TNF--TGF-

  • resting state

    primed state-- IFN-

    activated state-- C3b+LPS+IFN-

  • CR1CR3 CR4 FclgG FcFcR// IgA Fc LFA-1IL-8R

  • A. B. IgG-Ag FcR IgM CR

  • Dentritic Cells, DC) nave) TDCT CD1aCD11cCD83 MLRT

  • DC

    PBMC1% DCDC DCLC DC DC DC

  • DC DC

  • TIR T

  • DC a. b.FcR c. d. e.B7 f. DEC205

  • DC a.MHC -III b.DC-CK T c. d.

    T

  • T BIgB

    :

  • NK(natural killer cell)(large granular lymphocyte, LGL TB-- TCRIg TCR-mIg-CD56+CD16+ --- NKCTLTh IFN510 12 NK

  • Human NK cell

  • NNK

  • NK 1. 2.T --- A.NK --- CD3+NK --- CD3- B.NKT a. --- CD16 CD16+CD4-CD8- T CD16+CD4-CD8- NK b. --- NKCD56 CD3-CD4-CD8-CD56+ NK CD56- T 3. CD34+CD7+NKCD2+CD16+CD56+

  • NKHLA 1.KIR IgKIR2DKIR3D ITIMKIR2DLKIR3DLKIR2DSKIR3DSITAMDAP12NK

  • 2.KLRCD94/NKG2ACD94/NKG2C3.KIRKLR

  • NKCR MHC-I+NKNKMHC-I-KIRABMHC-IKAR NK(KIR)MHC I MHC I NK

  • NK 1. 1 A./ B.Fas/FasL C.TNF-TNFR-

  • 2ADCC FcRNK+ IgG( )

  • 2. IFN-TNF-GM-CSFIL-3M-CSF A.TBAPC B.

  • Antibody Dependent Cell-Medicated CytotoxicityADCCADCCIgGVFcNKFcRIgG

  • NKCR NKCR IgNKCR KIR H2N NH2 -S-S- HOOC COOHITIMITIM COOHNH2ITIM: immunoreceptor tyrosine-based inhibitory motifs

  • NK CD2CD16FcRIIICD69ADCC (antibody-dependent cell-mediated cytotoxicity)Target cell

  • NK cells and their activation

  • Electron micrograph of an NK cell

    High power view of an NK cell granule containing perforin, granzymes, and other mediators

  • Phase contrast micrograph of NK cell attacking a target cell projection.

    An "NK" cell (N) attached to a "target" cell "T".. The NK cell will kill the now helpless target cell quickly, by the injection of deadly perforin

  • NKT NK1.1TCR-CD3TCD4CD8TCD4TTCRTCRTCR CD1MHC

  • 1---FasL 2---NK1.1+T + DC + M a.DC+MIL-12NK1.1+TIFN-+IL-12Th0Th1 b.NK1.1+TIL-4Th0Th2 IgEIgG1

  • . 1.FcR 2.IL-5CCchemokines, MCP-3

  • A.,major basic protein B. C.IL-3IL-5 1. 2.

  • (mast cell)(basophils) ---0.2% --- , 1.FcRIgEFc 2.C3aR C5aR

  • 1.IgE-Ag FcR 2.C3aC5a

  • A. histamineC4leukotriene C4 D2prostaglandine D2 B. TNF IL-8PAF

  • A. B.IgE C.

  • 1.2.3.-

  • NKT NKTB1

  • 1.2.3.

  • 0-4h >96h APC4-96h NK

  • 0-4hM

  • 4-96hIL-8IL-6IL-1MCP-1(-1)LTB4(B4)PAF()PGE(E)-IFNTNF,IL-12

  • 96hAPC antigen-presenting cell

  • 1.pattern recognition receptor PRRpathogen associated patternPAMP2.Toll

  • inflammation (acute inflammation)(chronic inflammation) -1 -

  • PEICAM

  • 5- C3a C5aPGD2 LTC4LTD4TXEPAFN- PAFC5aLTB4PAF IL-8

  • NKADCC

    IgEFcIgE

  • (ADCC)NKIgGFc(CDl6FcrR)IgGFcNKFcrRNKIgGNKADCC

  • 1. To1124(TLR2TLR4)G-G+TLR2G+TLR4G+

  • 2. ---