Supplementary Data

New designed special cells for Raman mapping of the disintegration process of

pharmaceutical tablets

Tereza Čapková-Helešicováa,b,c, Tomáš Pekárek*b, Marek Schöngutb Pavel Matějkac

aDepartment of Analytical Chemistry, University of Chemistry and Technology Prague,

Technická 5, 166 28 Prague 6, Czech RepublicbZentiva, k.s., Praha, U Kabelovny 130, 102 37 Prague 10, Czech RepubliccDepartment of Physical Chemistry, University of Chemistry and Technology Prague,

Technická 5, 166 28 Prague 6, Czech Republic

* Corresponding author: Tomáš Pekárek, Tel.: +420 267 243 660,

E-mail: Tomas.Pekarek@zentiva.com

Table S1

Experimental parameters for on-line Raman mapping disintegration

The SC-MS method (with media suction) was tested to reveal if there was any spectral

interference during the measurement in medium. It was found the only difference is in higher

level of noise in the spectra measured in the medium. This was confirmed by the

measurement of pure lactose solution, where no bands assigned to this excipient were

observed (Fig. S1). Thus, arrangement with suction might be suitable for cases when spectral

interference of dissolved material takes place and this contribution cannot be suppressed by

mathematical spectral treatment.

Measurement mode StreamLine

Exposure time (s) 2.0

Step size (µm) 5.3

Objective magnification 40x

Laser power reduction (%) 100

Spectral range (cm-1) 1800 ‒ 730

Sampling area 200x200

Map acquisition time (min) 15

Fig. S1 Raman spectra of different pure D-lactose monohydrate solutions measured with 40x objective at three levels; A) the objective above the level of the solution, B) the objective at the level of the solution, C) the objective immersed in the solution

In our case, we also observed larger area during the disintegration process with 20x

objective in the off-line arrangement several times. This arrangement allowed faster mapping

of the larger area of the disintegrated tablets (Fig. S2), but with lower lateral resolution. The

area percentages of ibuprofen and lactose components are provided for 20x and 40x

objectives.

Fig. S2 Off-line Raman mapping of IBU–LAC tablets (φ% IBU 0.2, φ% LAC 0.8) disintegration using SC in distilled water, A) the objective 40x, the map size 200x200, B) the objective 20x, the map size 360x330, C) photos of tablet disintegration residues; red color represents IBU and green color is for LAC