SC K KH C BN

Copyright 2008 by LTD

I. L thuyt chung v sc k ctn Sc k :

Chromatogram

Time

Detector

Column

Injector

Sample Injection

ABAB

B

AB

A B

Peak A Peak B

1. Cc thng s c bnn Thi gian lu:

Retention Time (tr)n Th tch lu: Retetion

Volume (Vr)n B rng peak:

Baseline Width (W)n Thi gian lu cht:

Void Time (tm)n Th tch cht: Void

Volumn (Vm)

a. phn gii Resolution(R)

n V d: vi R = 0.5 th phn din tch b che ph l 0.13%

n Cc k thut nng cao phn gii:a) phn gii ban ub) Nng cao phn gii da vo hiu nng ctc) Nng cao phn gii da vo chn lc ct

b. H s dung lng Capacity factor (k)

k o c kh nng lu gi ca pha tnh i vi cht ha tan.

tr = tr - tm: thi gian lu hiu chnh

c. chn lc ct Column Selectivity

n H s chn lc Selectivity factor (a): l t s ca cc hs dung lng ca hai cht ha tan

d. Hiu nng ct Column Efficiency

n a l thuyt Theoretical plate

N: s a l thuyt

L: chiu di ct

H: chiu cao a l thuyt

2. Ti u ha phn gii

a. S dng k ti u ha phn gii

b. S dng chn lc ti u ha phn gii

c. S dng hiu nng ct ti u ha phn gii

Cc h s thc nghim tc ng n s bnh rng mi sc k. a hng-Mutiple Paths Khuych tn dc-Longitudinal Diffusion S truyn khi-Mass Transfer

n Phng trnh Van Deemter

III. Sc k kh

n S khi ca mt my sc k kh in hnh

1. Pha ng

n Vic la chn loi kh mang ph thuc vo u d thit b.

n Tc pha ng: Ct nhi: 25-150 mL/min Ct mao qun: 1-25 mL/min

2. Ct sc kn Ct nhi: 2-6 m chiu di x 2-4 mm ng knh trong x 37-44 mm

hoc 250-354 mm

a. Sc k lng kh Gas-Liquid Chromatography (GLC)

n Ct mao qun WCOT Wall-coated open

tubular columns SCOT Support-coated open

tubular columns PLOT Porous-layer open

tubular columns

WCOT SCOT PLOT

b. La chn pha tnh cho GLC

3. B phn tim mu

OnOn--column column InjectorInjector

SplitSplit//Splitless Splitless InjectorInjector

PTV InjectorPTV Injector Packed and Packed and widewide--boreborecolumnscolumns

Cc k thut tim thng dngSplit Splitless Direct vaporizing forwide bore capillary column

Direct vaporizing forpacked column

Car

rie r

gas

Split

Sept

um P

urge

column

4. u dn Flame Ionization Detector (FID): s dng cho cc hp cht hu

c

n Thermal Conductivity Detector (TCD): s dng cho cc kh v c v cc hp cht hu c nng cao

n Electron Capture Detector (ECD): s dng cho cc hp cht i lc in t

n Flame Photometric Detector (FPD): s dng cho cc hp cht hu c sulphur v phosphorus

n Flame Themionic Detector (FTD): s dng cho cc hp cht hu c nitrogen v phosphorus

5. ng dng nh lngn Mi trng:

Phn tch cc cht nhim hu c trong khng kh, nc, nc thi.

Phn tch cc hp cht hu c d bay hi.

n Y t: phn tch thuc

n Cc sn phm tiu dng: phn tch mi, gia v, hng liu.

n Cng nghip du m: phn tch cc sn phm du m, bao gm xng du, nht.

6. ng dng nh tnhn Khi s dng GC vi u d MS hoc FT-IR, thng tin ph c dng

nh danh cc cht.n Khi s dng cc u d thng thng, phi s dng cc phng php

khc: Thm vo mu mt lng cht phn tch kh nghi v theo di s tng ca

chiu cao peak. So snh thi gian lu ca mu chun v mu th trong cng mt iu kin

phn tch.

7. nh gin Thang hot ng:

FID v TCD c th dng cho mt lng ln mu phn tch. Cc u d khc nh ECD, MS yu cu mt lng nh cht phn tch

(~ pg)n ng: khong 1-5%.n chnh xc: khong 1-5%.n nhy: TCD

Thank you!