Metallothionein induction and heavy metal accumulation in white shrimp Litopenaeus vannamei exposed to cadmium and zi

ncJui-Pin Wua, Hon-Cheng Chenb,*

Comparative Biochemistry and Physiology, Part C 140 (2005) 383 – 394南美白對蝦暴露於鎘及鋅下之金屬硫蛋白誘發與

重金屬蓄積研究

Speaker:鐘偉銓

Introduction

Organism

Pollutiion

Water

Materials and methods

Control 0.2 mg Cd/l 0.3 mg Zn/l

After 3 days

Hepatopancreas

Sephadex G-75

Centrifuge

0 , 0.1 , 0.2 mg Cd/l

Hepatopancreas(h),gills(g),muscles(m)

Polarized Zeeman atomic absorption spectrophotometer

0, 0.05 , 0.2 mg Zn/l

Results &Conclusion

s

Normal

Cd

Zn

h

g

m

Cd Zn

Discussion

The third protein peak is a Zn peak.(Wong and Rainbow,1986)

This indirect method with a direct one revealed that they yielded similar results and trends.(Pederson et al., 1997)

Use MT for monitoring and evaluation the heavy metal pollution was reasonably considered.(Bainy, 2000)

Thanks