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8/13/2019 NG DNG VI SNG TRONG CHIT XUT POLYPHENOL T CY CH (1)
1/24
PHNG PHP CHIT XUT
C H TR VI SNGNG DNG VI SNG TRONG CHIT
XUT POLYPHENOL T CY CH
!C "#CH C$% "& T'I
Cy ch Camellia sinenssis O.Ktze ( Thea chinensis Seem.) c s dng
t rt lu trng dn g!"n c#ng nh trng y h$c %h&ng 'ng. *n y y h$c h!+n
,! -u"n tm nh!u /n t0nh cht ch1ng 2y h3" c4" c5c h% cht %ly%henl c3
ch6" trng cy ch7 nh!u ngh!8n c6u c th9c h!+n : t;m g!
%h*n %ly%henl trng cy ch c th9c h!+n @Gng nh!u %h&ng %h5% nh ch!/t
2ut hH! lu7 ch!/t 2ut hI tr s!8u m7 ch!/t 2ut ngm J!+tD h&ng %h5% ch!/t
2ut c3 hI tr E! s3ng (L!crM"EeN"s!ssted e2tr"ct!n) c 6ng dng ngFy
cFng rPng r! trng ngh!8n c6u h3" th9c EQt c#ng nh trng lRnh E9c ch!/t 2ut
h% cht trng nghFnh c ET! c5c u !:m E thU! g!"n ch!/t 2ut7 t!/t J!+m
dung m! EF n?ng lng7 t0nh ch$n l$c7 0t
8/13/2019 NG DNG VI SNG TRONG CHIT XUT POLYPHENOL T CY CH (1)
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( PH)N T*NG +U%N
(( ,HI NI-! V& VI SNG.(/
((( VI SNGV0 1234(m!crNnde7 m!crM"Ee) lF s3ng c9c ng[n h"y c\n g$! lF s3ng s!8u
t*n7 s3ng ]A^ (]ltr" A!gh ^re-uence M"Ee).
_rng %hY !+n t (electrm"gnet!c s%ectrum)7 E! s3ng nGm V Jh
8/13/2019 NG DNG VI SNG TRONG CHIT XUT POLYPHENOL T CY CH (1)
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_rng lRnh E9c BSL (Bndustry7 Sc!ence7 Led!c!ne) tr8n th/ g!T! ngU! t" -u!
Tc s dng c5c l,! E! s3ng c3 t*n s1 b7i`7``7ibiLAz. _rng c5c t*n
s1 tr8n7 ch c3 t*n s1 i` LAz lF c s dng rPng r!.
A*u h/t c5c l\ E! s3ng g!" dng u s dng t*n s1 i`LAz7 t*n s1 nFy c\nc dong trng cng ngh+ nng s
8/13/2019 NG DNG VI SNG TRONG CHIT XUT POLYPHENOL T CY CH (1)
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((5 C S6 C$% CHIT VI SNG
S9 un n3ng @Gng E! s3ng lF mPt t!/n tr;nh lFm t?ng nh!+t P c4" EQt cht mPt
c5ch c @!+t. _!/n tr;nh nFy Jhng %h thuPc EF s9 dvn nh!+t c4" @;nh ch6" EF
EQt cht. S9 t?ng nh!+t cc @P t6c thU! c4" EQt cht lF d s9 -u"y lwng c9c(d!%le rt"t!n) EF s9 dvn truyn !n (!n!c cnduct!n). '3 lF i c& ch/ c& sV c4"
s9 chuy:n n?ng lng t E! s3ng s"ng EQt cht.
Zh!+t s!nh r" d s9 783 9:;3 lF J/t -u< c4" s9 t?ng trV Jh5ng c4" m!
trUng ch1ng l,! s9 d>ch chuy:n c4" c5c !n trng trUng !+n t.
C\n c& ch/ ?;@< AB34 lF
-u5 tr;nh Y! hTng c4" mPt %hn t
%hn c9c the ch!u c4" !+n trUng.
_h0 d t,! t*n s1 i`LAz7 !+n
trUng x c4" E! s3ng Y! ch!u
7.b`m# l*ns.
T! t5c Png c4" !+n trUng7
c5c %hn t lwng c9c c3 Jhuynh
hTng s[% 2/% the ch!u !+n
trUng. 3 trng !+n trUng 2"y
ch!u t*n s1 rt c" (LAz) s gy r"
s9 25 trPn m" s5t ET! EQn t1c rt lTn
g!=" c5c %hn t7 3 ch0nh lF nguHn
g1c s9 n3ng l8n c4" EQt cht.
Zh=ng h% cht cFng %hn c9c cFng m"u n3ng dT! s9 ch!/u 2, c4" E! s3ng.! s3ng J0ch h,t nh=ng %hn t %hn c9c7 c @!+t lF nTc. ZTc @> un n3ng
@V! s9 h% thu E! s3ng EF @1c h&!7 t, r" 5% sut c" t,! n&! t5c dng7 lFm ch
nTc d! chuy:n t tm EQt cht r" @ mt c4" n3. Zguy8n t[c nFy c 6ng dng
trng s9 sy ch!/u 2, @Gng E! s3ng.
T! mPt c& cu c3 P @t 1! 26ng c"7 %hn t nTc c3 P %hn c9c m,nh7 d
3 nTc lF mPt cht rt l tVng7 rt d un n3ng @Gng E! s3ng. ZgF! r"7 c5c
_r"ng
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nh3m >nh ch6c %hn c9c nhq NOA7 NCOOA7 NZAi... trng c5c h% cht h=u c&
c#ng lF nh=ng nh3m ch>u s9 t5c Png m,nh c4" trUng !+n t.
((F U NHC "I! C$% VI SNG
; 0JK @ M0 1234
Khng c3 -u5n t0nh nh!+t.
A!+u sut ch!/t c" h&n s ET! mPt s1 %h&ng %h5% ch!/t thng thUng.
S d s dng7 "n tFn EF @ t, E! s3ng lF
Jhng nhf : c3 4 cng sut.
Zh!+t P s! c4" c5c dung m! ,t c rt nh"nh7 c3 th: gy nY
((Q NG DNG C$% VI SNG
T! nh=ng t0nh n?ng Et trP! mF n3 c3 c7 E! s3ng c 6ng dng rPng r!
EF t!n cQy. 'c @!+t7 trng c5c %h
8/13/2019 NG DNG VI SNG TRONG CHIT XUT POLYPHENOL T CY CH (1)
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!
cht m"ng r[n.
_rng thng t!n l!8n l,cq Xluetth EF c5c chupn Bxxx `i.bbg7
`i.bb@ EF `i.bb".
_rng cng ngh!+%q
Sy Jh th9c %hpm.
S5t trong th9c %hpm.
Sy gI.
(( L VI SNG
LPt l\ E! s3ng b hc i`LAz c3 a @P %hQn ch0nhq L"gnetrn7 1ng dvn
s3ng7 l\ng l\.
_r"ng k
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!@439:>3nguHn %h5t s3ng !+n t7 @P %hQn chuy:n h3" n?ng lng
!+n thFnh n?ng lng E! s3ng. '3 lF b 1ng c rWt chn Jhng7 t,
thFnh @V! i h;nh tr Hng tm ("nd EF c"td).
34 783 1234lF @P %hQn dvn E! s3ng t m"gnetrn ! EF l\ng l\.
L34 AlF n&! ch6" mvu EQt ch>u s9 ch!/u 2, E! s3ng. 5ch l\ lFm
@Gng J!m l,! n8n c3 Jh< n?ng %h
c5ch l"n truyn EF cPng hVng c4" s3ng.
'!+n trUng c %hn %h1! u trng 1ng dvn s3ng EF c< ng"y trng mvu EQt7
ch h!+u sut c" h&n Jh! s dng l\ " c5ch.
_r"ng j
8/13/2019 NG DNG VI SNG TRONG CHIT XUT POLYPHENOL T CY CH (1)
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Hnh minh ha cc loi l microwave
_r"ng
8/13/2019 NG DNG VI SNG TRONG CHIT XUT POLYPHENOL T CY CH (1)
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(5 T*NG +U%N V& CY CH V' NH! POLYPHENOL
(5( PHN LOZI TH[C V\T ] "^C "I! SINH THI V' PHN
_.5/
_8n Jh" h$c Camellia sinensis O.Ktze ( Thea chinensis Seem.). C\n c3 t8n
g$! Jh5c lF cy _rF.
_huPc h$ Chq Theaceae.
XP %hQn thUng dong lF @W% EF l5 ch nn (^l!um _he"e)7 s" Jh lFm
thu1c7 thUng g$! lF ch h&ng h"y ch _Fu. C\n g$! lF ch d!+%.
M t cy:
Ch lF mPt cy Jhfe7 m$c h"ng EF
Jhng c[t 2{n c3 th: c" tT! b`m h"y h&n
n="7 Ung J0nh thn c3 th: tT! m6c mPt
ngU! m Jhng 2u:. '! Jh! m$c thFnh rng
gI tr8n nW! 5 c". Zhng trng Jh! trHng t"
thUng ngU! t" c[t 2{n : t!+n E!+c h5! n8n
ch c" nht lF im. Zh!u cFnh m ng"y t
g1c. 5 m$c s le7 Jhng rng. A" t tr[ng7
m$c V J l5 mo! rt th&m7 nh!u nh>. |u< lF
mPt n"ng thUng c3 @" ng?n7 nhng ch c\n
mPt h,t d c5c h,t Jh5c @> te !. |u< Jh"!@Gng l1! c[t ng"ng7 h,t Jhng %h! nh#7 l5 m*m lTn7 c3 ch6" d*u.
_r"ng
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c im phn b:
Ch lF mPt cy c3 nguHn g1c t _rung |u1c. Zhn dn _rung |u1c @!/t
dong ch t i`` n?m _CZ7 s"u tT! ZhQt X
8/13/2019 NG DNG VI SNG TRONG CHIT XUT POLYPHENOL T CY CH (1)
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x%!g"llc"tech!n a g"ll"te (xC) x%!g"llc"tech!n (xC)
x%!c"tech!n a g"ll"te (xC) x%!c"tech!n (xC)lJ"l!dq c"e!n lF "lJ"l!d ch0nh (i7 7 ). _rng l5 th6 nht c3 a7a7
l5 th6 h"! c3 7i`7 l5 th6 @" c3 a7`7 l5 th6 t c3 i7b`7 cu1ng ch c3 `7ak.
ZgF! r" c\n c3 mPt lng rt nhf the%hyll!n (`7`i `7`)7 the@rm!n `7`7
"den!n EF 2"nth!n. _hFnh %h*n c"e!n Jhng @> @!/n Y! trng -u5 tr;nh ch/ @!/n.
C"e!n
rte!n EF "c!d "m!nq %rte!n thUng J/t h% ET! t"n!n. _rng ch ngU! t"
t;m thy bj "c!d "m!n. C5c "c!d "m!n nFy J/t h% ET! Ung EF t"n!n t, r"
"ldehyde c3 mo! th&m c4" ch en EF lFm ch ch 2"nh c3 d E> t1t.
_r"ng bb
8/13/2019 NG DNG VI SNG TRONG CHIT XUT POLYPHENOL T CY CH (1)
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luc!d EF %ect!n.
^l"En!dq c"merl7 -ue2!tr!n7 m!r2et!nD
_!nh d*u.
!t"m!n 7 Xb7 Xi7 EF nh!u nht lF E!t"m!n C.
ZgF! r" c\n c3 menq "m!l"se7 glucs!d"se7 %rte"se7 %er2!d"se EF %lyN
%henl2!d"se EF c5c mu1! E c&.
(5F CU TRaC ] T#NH CHT Lb H% C$% NH!
POLYPHENOL "cNH HdNG CHIT XUT TRONG CY
CH./
A% cht %ly%henl trng cy ch thuPc nh3m t"nn!n Jhng th4y %hn c
h"y c\n g$! lF nh3m t"nn!n ngng t7 t"nn!n %yrc"tech!n.
C5c h% cht nFy c3 t0nh cht chung c4" t"nn!n.
(5F( Lb T#NH
A% cht rt %hn c9c7 d t"n trng dung m! lF J!m lng7 cHn lng. _"n
trng cHn7 "cetn7 etyl "cet"te. Kh3 t"n trng nTc7 Jh3 J/t t!nh. Khng t"n trng
dung m! J{m %hn c9c nh n he2"n7 ether d*u hf"7 @enzen7 d!etyl ether.
T! c5c dung d>ch nTc c4" %rte!n nh "l@um!n7 gel"t!nq t, %h6c t4" @n
(c3 t0nh thuPc d").
_r"ng bi
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hY B EF ]q c"tech!n c3 x ba` b` ( Eong i`nm)7 e%!c"tech!n c3 x
k j` ( Eong ijinm).
y s?n se n!8m m,c7 J0ch 6ng d, dFy.
(5F5 H% T#NH
hch nTc @rm.
Khng @> th4y %hn @V! t"nn"se7 "c!d hc J!m n3ng mF ngng t t, thFnh
%hl@"%hen (f t"nn!n).
hn ng E! thuc thSt!"sny (^rml ACl Qm c t l+ iqb ) t, cht
trong h% mFu f g,ch Jhng t"n trng nTc.
_r"ng ba
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5 PH)N TH[C NGHI-! e CHIT XUT POLYPHENOL
_fNG PHNG PHP VI SNG.g/
5( NGUYhN LI-U V' PHNG PHP
5(( NGUYhN LI-U
Ch 2"nh (@W% EF i7a l5 nn) V Eong X
8/13/2019 NG DNG VI SNG TRONG CHIT XUT POLYPHENOL T CY CH (1)
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b``g ch ( c @t h,t enzyme) &c c[t EF 2"y nhf7 s"u 3 trPn ET!
dung m! ET! t l+ th0ch h%. S"u 3 ch!/u E! s3ng (mPt %hWt ch!/u 2,7 h"! %hWt
ngng) : g!= ch nh!+t P Jhng t?ng l8n h&n ``C. S"u 3 : nguP! V nh!+t P
%h\ng7 l$c7 EF @nh lng ly%henl tFn %h*n. ly%henl %hch mFu EFng c4" %ly%hs%h"tetungsten"te EF mlyd"te) trng m!
trUng J!m y/u t, thFnh mFu 2"nh Qm. |uy tr;nh c th9c h!+n nh s"uq
`.ml c4" mvu (s dng nTc ct lFm mvu tr[ng) c ch EF @;nh >nh
m6c b`ml7 h\" t"n ET! 7ml nTc ct. S"u 37 th8m `.iml thu1c th ^l!n7 `.ml
dung d>ch Z"iCOa@ h\". [c u7 th8m nTc EF /n E,ch EF : y8n trng
E\ng b g!U. S"u 37 dung d>ch c V @Tc s3ng jinm @Gng m5y -u"ng
%hY enM"y k` ]N!s.
ng %ly%henl tFn %h*n c t0nh d9" EF P h% %h c4" mvu h%
cht EF Ung hH! -uy tuy/n t0nh s dng "c!d g"ll!c lFm cht chupn. K/t -unh lng c4" %ly%henl tFn %h*n c -uy E "c!d "ll!c.
5(Q "cNH LNG C%TECHINS
C"tech!n c >nh lng @Gng %h&ng %h5% AC c s dng trng c5c
tF! l!+u cng @1 trTc y c th!/t J/ l,! ch %ho h%. |u5 tr;nh %hn t0ch c
th9c h!+n ET! m5y AC bb`` g!l"nt7 CPt %h" nh trng
su1t -u5 tr;nh %hn t0ch lF bml%hWt.
_r"ng b
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|u5 tr;nh s[c J r" g!
8/13/2019 NG DNG VI SNG TRONG CHIT XUT POLYPHENOL T CY CH (1)
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Biu 1: nh h!n" c#a nn" $ e%hanol %ron" &u %rnh chi'% (u)% cc
*ol+*henol. T, l- n"u+n li-u/ 0un" mi: 1:2 3"/ml45 %h %6ch 0un" mi 178ml5 %h9i
"ian chi'u ( 2 *h%5 n"un cn" su)% ;88 th9c ngh!+m trng th!/t J/ th0 ngh!+m nFy. '!u nFy c3 th: g!
c lF d trng th!/t J/ th0 ngh!+mnguy8n l!+u s dng lF ch c sy Jh
(P pm Nj)7 c\n trng th!/t J/ th0 ngh!+m nFy s dng nguy8n l!+u t&! (P pm
jN`).
_r"ng bj
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Biu =: nh h!n" c#a %, l- n"u+n li-u/0un" mi %ron" &u %rnh chi'%
(u)% *ol+*henol. >un" mi e%hanol 28?5 %h %6ch s@ 0An" 178 ml5 %h9i "ian chi'u
(: 2 *h%5 cn" su)% n"un ;88
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Biu : nh h!n" c#a %h9i "ian chi'u ( ln &u %rnh chi'% (u)%
*ol+*henol. >un" mi e%hanol 28?5 %h %6ch 0un" mi 178ml5 %, l- n"u+n
li-u/0un" mi 1:2 "/m".
55Q HI-U SUT CHIT VdI THwI GI%N LIhN TC
Biu : Hi-u su)% chi'% %ron" %h9i "ian lin %Ac. >un" mi: e%hanol
28?5 %h %6ch 0un" mi 178ml5 %, l- n"u+n li-u/0un" mi 1:2 "/ml5 %h9i "ian
chi'u ( 2 *h%5 cn" su)% m+ ;88ch ch!/t Jhng Y! mFu
Jh! th8m EF! g!$t dung d>ch thu1c th ^eClaaACl `.bZ (mFu 2"nh r8u s 2uth!+n n/u c3 s9 h!+n d!+n c4" %ly%henl trng d>ch ch!/t) : ch!/t J!+t tFn @P
_r"ng b
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%ly%henl trng nguy8n l!+u. Biu ch thy h!+u 2ut ch!/t trng l*n ch!/t
*u t!8n Jhnh t0nh EF >nh lng. _rng s[c J H AC7 @8n
c,nh %e"J c n3! V tr8n7 mPt EF! %e"J %h c#ng c %h5t h!+n7 !u 3
ch thy -u5 tr;nh ch!/t 2ut c#ng ch!/t r" mPt EF! thFnh %h*n c"tech!n Jh5c t
trng cy ch.
DEc FG HIC c#a 0Jch chi'% (u)% %K cL+ chM "hi nhNn ! OPc sQn" =8nm.
A,t cht C xC xC xC C"e!n
_r"ng i`
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_hU! g!"n lu t k.aab j.`j` .kb j.ik k.ka
AFm lng ch!/t c ( t0nh
the nguy8n l!+u Jh)`.a `.a b.i bi. a.a
55g SO SNH ,T +Up CHIT XUT _fNG PHNG PHP
CHIT C H TR VI SNG VdI CC PHNG PHP CHIT
XUT ,HC
Zh=ng -uy tr;nh ch!/t 2ut Jh5c nh q %h&ng %h5% ch!/t @Gng c5ch un
hH! lu7 ch!/t 2ut c3 hI tr s!8u m7 EF ngm J!+t V nh!+t P %h\ng c t!/n
hFnh : s s5nh J/t -u< ET! %h&ng %h5% ch!/t 2ut c3 hI tr E! s3ng. K/t -u