BIOCHEMISTRYBIOCHEMISTRY

万晴姣School of biological technology

and engineering

教 材教 材 ::《 biochemistry 》（第二版，影印版） Reginald H. Garrett & Charles M.Grisham 高等教育出版社

主要参考书目主要参考书目 ::1 ．《生物化学》（第三版） 王镜岩主编 高等教育出版社2. 《生物化学》（上、下册） 沈彤，王镜岩主编 高等教育出版社3 ．《 Instant Notes in Biochemistry 》（第二版，先锋版） B.D.Hames & N.M.Hopper 科学出版社4 ．《普通生物化学》（第三版） 郑集，陈钧辉编著 高等教育出版社

生物化学是生命的化学，是研究微生物、动物、植物和人体的化学以及生命过程中化学变化的一门科学。

I. The Early Years

• Antoin Lavoisier (1743-1794)• Joseph Louis Gay-Lussac (1778-1850)• Friederich Wohler 1828 H2N-CO-NH2 • Hoppe Seyler 1877 (Zeitschrift fur Physiologesche Chemie )• Carl A. Neuberg 1903 biochemistry• W. Kuhne enzyme• Emil Fischer 1894 enzyme specificity

II. Rapid Progress in Biochemistry

• 1926 James B. Summer urease• 1937 Hans A. Krebs TCA cycle• 1951 Linus Pauling & Robert Corey a-helix• 1953 Frederich Sanger insulin• 1953 James D. Watson & Francis H.C. Crick DNA double-helix

Part I . Molecular Components of Cell

Chapter 4 Amino Acids

4.1 Amino Acids: Building Blocks of Proteins

4.1.1 Common Amino Acids

• Nonpolar Amino Acids

• Polar,Uncharged Amino Acids

• Acidic Amino Acids

• Basic Amino Acids

Ala Val Leu Ile Pro Met Phe Try

Gly Ser Thr Cys Asn Gln Tyr

Asp Glu

Lys Arg His

4.1.2 Uncommon Amino Acids

4.1.3 Amino Acids Not Found in Proteins

4.2 Acid-Base Chemistry of Amino Acids

(pI)

(pI)

(pI)

4.3 Reactions of Amino Acids

4.3.1 Carboxyl and Amino Group Reactions

4.3.2 The Ninhydrin Reaction

4.3.3 Specific Reaction of Amino Acid Side Chains

4.4 Optical Activity and Stereochemistry of Amino Acids

4.5 Spectroscopic Properties of Amino Acids

• Ultraviolet Spectra 280nm

• Nuclear Magnetic Resonance (NMR) Spectra

4.6 Separation and Analysis of Amino Acid Mixtures

Chromatographic Methods

• Ion Exchange Chromatographic

• Gas Chromatographic (GC)

• High-performance Liquid Chromatographic (HPLC)

Operation of a cation exchange column ,separating a mixture of Asp,Ser,and Lys.

The separation of amino acid on a cation exchange column.

HPLC